(Circular) (Six-base) MAPSORT of: pFN21AB7200.seq Check: 5160 from: 1 to: 5998 pFN21AB7200 5998 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2350 3261 2350 Size: 911 5087 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3341 1937 Size: 1404 4594 Acc65I G'GTAC_C Cuts at: 3319 3319 Size: 5998 AclI AA'CG_TT Cuts at: 1563 4125 4498 1563 Size: 2562 373 3063 Fragments arranged by size: 3063 2562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3109 4061 5445 5713 1632 Size: 1477 952 1384 268 1917 Fragments arranged by size: 1917 1477 1384 952 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 4897 5874 1203 Size: 3694 977 1327 Fragments arranged by size: 3694 1327 977 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 4727 4727 Size: 5998 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5998 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2524 1184 Size: 1340 4658 AlwNI CAG_nnn'CTG Cuts at: 2728 2740 3741 5313 2728 Size: 12 1001 1572 3413 Fragments arranged by size: 3413 1572 1001 12 ApaI G_GGCC'C Cuts at: 2119 2126 2167 2267 2119 Size: 7 41 100 5850 Fragments arranged by size: 5850 100 41 7 ApaLI G'TGCA_C Cuts at: 2363 2529 4055 5211 2363 Size: 166 1526 1156 3150 Fragments arranged by size: 3150 1526 1156 166 ApoI r'AATT_y Cuts at: 1493 3307 3533 3685 5794 1493 Size: 1814 226 152 2109 1697 Fragments arranged by size: 2109 1814 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 2118 2898 3323 1870 Size: 73 175 780 425 4545 Fragments arranged by size: 4545 780 425 175 73 BamHI G'GATC_C Cuts at: 3328 3695 3328 Size: 367 5631 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2242 2934 3319 4774 618 Size: 325 217 627 455 692 385 1455 1842 Fragments arranged by size: 1842 1455 692 627 455 385 325 217 BanII G_rGCy'C Cuts at: 1391 2119 2126 2167 2267 2381 2573 2840 3317 Size: 728 7 41 100 114 192 267 477 Cuts at: 3317 1391 Size: 4072 Fragments arranged by size: 4072 728 477 267 192 114 100 41 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2416 2617 928 Size: 971 517 201 4309 Fragments arranged by size: 4309 971 517 201 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4189 4223 1903 Size: 34 50 34 2168 34 3678 Fragments arranged by size: 3678 2168 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3890 5100 3890 Size: 1210 4788 BclI T'GATC_A Cuts at: 1548 2388 1548 Size: 840 5158 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2774 2783 4609 Size: 107 122 71 631 605 1102 9 1826 Cuts at: 4609 136 Size: 1525 Fragments arranged by size: 1826 1525 1102 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 2149 3090 5993 2149 Size: 941 2903 2154 Fragments arranged by size: 2903 2154 941 BlpI GC'TnA_GC Cuts at: 3415 3415 Size: 5998 Bme1580I G_kGCm'C Cuts at: 1790 2119 2126 2167 2257 2267 2367 2533 2565 Size: 329 7 41 90 10 100 166 32 Cuts at: 2565 2695 4059 5215 1790 Size: 130 1364 1156 2573 Fragments arranged by size: 2573 1364 1156 329 166 130 100 90 41 32 10 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5998 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2427 2520 3729 Size: 416 53 83 399 24 1010 93 1209 Cuts at: 3729 4687 442 Size: 958 1753 Fragments arranged by size: 1753 1209 1010 958 416 399 93 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2101 2276 2393 2690 2750 2768 3048 3077 Size: 862 175 117 297 60 18 280 29 Cuts at: 3077 3257 4658 1239 Size: 180 1401 2579 Fragments arranged by size: 2579 1401 862 297 280 180 175 117 60 29 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2417 2633 4123 4988 5286 2417 Size: 216 1490 865 298 3129 Fragments arranged by size: 3129 1490 865 298 216 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4661 882 Size: 617 3162 2219 Fragments arranged by size: 3162 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3789 493 Size: 711 2585 2702 Fragments arranged by size: 2702 2585 711 BsaBI GATnn'nnATC Cuts at: 5992 5992 Size: 5998 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3805 4187 4822 275 Size: 53 83 186 936 2272 382 635 1451 Fragments arranged by size: 2272 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2928 4431 4829 5103 5250 5661 2928 Size: 1503 398 274 147 411 3265 Fragments arranged by size: 3265 1503 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2271 2301 2685 2715 2822 Size: 30 165 30 868 30 384 30 107 Cuts at: 2822 2852 1178 Size: 30 4324 Fragments arranged by size: 4324 868 384 165 107 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2164 2279 2693 1186 Size: 978 115 414 4491 Fragments arranged by size: 4491 978 414 115 BseYI C'CCAG_C Cuts at: 2075 2110 2260 2380 2786 2881 3045 3266 5201 Size: 35 150 120 406 95 164 221 1935 Cuts at: 5201 5697 2075 Size: 496 2376 Fragments arranged by size: 2376 1935 496 406 221 164 150 120 95 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2073 2494 3161 1163 Size: 573 337 421 667 4000 Fragments arranged by size: 4000 667 573 421 337 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3448 4209 5237 1540 Size: 275 186 1447 761 1028 2301 Fragments arranged by size: 2301 1447 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2367 2533 2573 4059 4144 5215 2367 Size: 166 40 1486 85 1071 3150 Fragments arranged by size: 3150 1486 1071 166 85 40 BsmI GAATG_Cn' Cuts at: 3509 3602 3509 Size: 93 5905 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2119 2126 2167 2257 2267 2367 2381 Size: 399 329 7 41 90 10 100 14 Cuts at: 2381 2533 2565 2573 2695 2840 3317 4059 4144 Size: 152 32 8 122 145 477 742 85 Cuts at: 4144 5215 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 477 399 329 152 145 122 100 90 85 41 32 14 10 8 7 BspEI T'CCGG_A Cuts at: 2928 5661 2928 Size: 2733 3265 BspHI T'CATG_A Cuts at: 3887 5617 3887 Size: 1730 4268 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2115 2350 3261 3353 844 Size: 1271 235 911 92 3489 Fragments arranged by size: 3489 1271 911 235 92 BsrBI CCG'CTC Cuts at: 1394 1823 3885 1394 Size: 429 2062 3507 Fragments arranged by size: 3507 2062 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4493 4667 65 Size: 1183 3245 174 1396 Fragments arranged by size: 3245 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2497 4642 4829 1700 Size: 110 687 2145 187 2869 Fragments arranged by size: 2869 2145 687 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5998 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5998 BssSI C'ACGA_G Cuts at: 4058 5070 4058 Size: 1012 4986 BstAPI GCAn_nnn'nTGC Cuts at: 794 2743 794 Size: 1949 4049 BstBI TT'CG_AA Cuts at: 2628 4802 2628 Size: 2174 3824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5998 BstXI CCAn_nnnn'nTGG Cuts at: 2786 2786 Size: 5998 BstYI r'GATC_y Cuts at: 1972 2149 3090 3328 3695 4080 4097 5549 5635 Size: 177 941 238 367 385 17 1452 86 Cuts at: 5635 5647 5993 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 941 385 367 346 238 177 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2950 3225 513 Size: 552 1885 275 3286 Fragments arranged by size: 3286 1885 552 275 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2187 487 Size: 683 56 961 4298 Fragments arranged by size: 4298 961 683 56 BtsI GCAGTG_nn' Cuts at: 2222 2501 2579 2653 3514 4308 4328 2222 Size: 279 78 74 861 794 20 3892 Fragments arranged by size: 3892 861 794 279 78 74 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5963 DraI TTT'AAA Cuts at: 3302 3302 Size: 5998 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5005 809 Size: 507 3689 1802 Fragments arranged by size: 3689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2469 3445 4334 Size: 54 1425 50 275 18 639 976 889 Cuts at: 4334 5963 8 Size: 1629 43 Fragments arranged by size: 1629 1425 976 889 639 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3445 1537 Size: 275 1633 4090 Fragments arranged by size: 4090 1633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2182 2625 3928 1353 Size: 829 443 1303 3423 Fragments arranged by size: 3423 1303 829 443 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2678 3149 4585 4959 5105 387 Size: 1064 138 1089 471 1436 374 146 1280 Fragments arranged by size: 1436 1280 1089 1064 471 374 146 138 EcoICRI GAG'CTC Cuts at: 2571 2571 Size: 5998 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2101 2276 2393 2690 2750 2768 3048 Size: 393 469 175 117 297 60 18 280 Cuts at: 3048 3077 3109 3257 4061 4658 5445 5713 1239 Size: 29 32 148 804 597 787 268 1524 Fragments arranged by size: 1524 804 787 597 469 393 297 280 268 175 148 117 60 32 29 18 EcoO109I rG'GnC_Cy Cuts at: 2115 2122 2163 2264 2870 2905 2115 Size: 7 41 101 606 35 5208 Fragments arranged by size: 5208 606 101 41 35 7 EcoRI G'AATT_C Cuts at: 3307 3685 3307 Size: 378 5620 FspI TGC'GCA Cuts at: 4504 4504 Size: 5998 HaeII r_GCGC'y Cuts at: 1678 5145 1678 Size: 3467 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2822 2854 4639 4671 4713 Size: 32 135 32 1250 32 1785 32 42 Cuts at: 4713 4745 1373 Size: 32 2626 Fragments arranged by size: 2626 1785 1250 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3342 3588 669 Size: 988 281 1404 246 3079 Fragments arranged by size: 3079 1404 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5998 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KpnI G_GTAC'C Cuts at: 3323 3323 Size: 5998 MfeI C'AATT_G Cuts at: 3597 3597 Size: 5998 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2853 3303 5112 5296 1305 Size: 1548 450 1809 184 2007 Fragments arranged by size: 2007 1809 1548 450 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2471 10 Size: 54 1425 343 639 3537 Fragments arranged by size: 3537 1425 639 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3110 3956 4315 4474 518 Size: 648 1944 846 359 159 2042 Fragments arranged by size: 2042 1944 846 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2079 2209 2215 2740 3045 3076 3414 4091 Size: 473 130 6 525 305 31 338 677 Cuts at: 4091 5239 5484 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 525 473 338 305 245 130 31 6 NaeI GCC'GGC Cuts at: 2499 2499 Size: 5998 NcoI C'CATG_G Cuts at: 513 1065 2950 513 Size: 552 1885 3561 Fragments arranged by size: 3561 1885 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5998 NgoMIV G'CCGG_C Cuts at: 2497 2497 Size: 5998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 3013 3115 3356 4901 1132 Size: 97 1784 102 241 1545 2229 Fragments arranged by size: 2229 1784 1545 241 102 97 PasI CC'CwG_GG Cuts at: 1348 2126 1348 Size: 778 5220 PciI A'CATG_T Cuts at: 4897 4897 Size: 5998 PflMI CCAn_nnn'nTGG Cuts at: 1108 2674 2986 1108 Size: 1566 312 4120 Fragments arranged by size: 4120 1566 312 PfoI T'CCnGG_A Cuts at: 2292 3054 2292 Size: 762 5236 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3224 3256 4067 4099 5580 5612 3224 Size: 32 811 32 1481 32 3610 Fragments arranged by size: 3610 1481 811 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5998 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PspOMI G'GGCC_C Cuts at: 2115 2122 2163 2263 2115 Size: 7 41 100 5850 Fragments arranged by size: 5850 100 41 7 PstI C_TGCA'G Cuts at: 830 2214 2361 3350 830 Size: 1384 147 989 3478 Fragments arranged by size: 3478 1384 989 147 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 PvuII CAG'CTG Cuts at: 2079 2209 2215 2740 2079 Size: 130 6 525 5337 Fragments arranged by size: 5337 525 130 6 SacI G_AGCT'C Cuts at: 2573 2573 Size: 5998 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SapI GCTCTTCn'nnn_ Cuts at: 2625 2625 Size: 5998 SbfI CC_TGCA'GG Cuts at: 3350 3350 Size: 5998 ScaI AGT'ACT Cuts at: 4246 5958 4246 Size: 1712 4286 SfcI C'TryA_G Cuts at: 826 1045 2210 2357 3346 4481 5162 5353 826 Size: 219 1165 147 989 1135 681 191 1471 Fragments arranged by size: 1471 1165 1135 989 681 219 191 147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5998 SmaI CCC'GGG Cuts at: 1872 2120 2900 3325 1872 Size: 248 780 425 4545 Fragments arranged by size: 4545 780 425 248 SmlI C'TyrA_G Cuts at: 820 1017 1943 2432 2648 4102 5003 5265 820 Size: 197 926 489 216 1454 901 262 1553 Fragments arranged by size: 1553 1454 926 901 489 262 216 197 SnaBI TAC'GTA Cuts at: 493 3789 493 Size: 3296 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 3922 5 Size: 47 3870 2081 Fragments arranged by size: 3870 2081 47 StuI AGG'CCT Cuts at: 1517 2020 1517 Size: 503 5495 StyI C'CwwG_G Cuts at: 513 1065 2043 2050 2805 2866 2950 3437 513 Size: 552 978 7 755 61 84 487 3074 Fragments arranged by size: 3074 978 755 552 487 84 61 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2107 2419 2473 2548 4025 4042 4195 Size: 1255 527 312 54 75 1477 17 153 Cuts at: 4195 4380 4825 325 Size: 185 445 1498 Fragments arranged by size: 1498 1477 1255 527 445 312 185 153 75 54 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4244 5956 96 Size: 274 80 33 51 1443 2267 1712 138 Fragments arranged by size: 2267 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2685 2717 5958 5990 2685 Size: 32 3241 32 2693 Fragments arranged by size: 3241 2693 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5998 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5998 XmaI C'CCGG_G Cuts at: 1870 2118 2898 3323 1870 Size: 248 780 425 4545 Fragments arranged by size: 4545 780 425 248 XmnI GAAnn'nnTTC Cuts at: 2624 4127 2624 Size: 1503 4495 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI