Restriction map for
pFN21AB7200
(Linear) (Six-base) MAPSORT of: KIBB7200.seq Check: 7096 from: 1 to: 1293
KIBB7200.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 345 1256 1293
Size: 345 911 37
Fragments arranged by size:
911 345 37
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1104 1293
Size: 1104 189
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 519 1293
Size: 519 774
AlwNI CAG_nnn'CTG
Cuts at: 0 723 735 1293
Size: 723 12 558
Fragments arranged by size:
723 558 12
ApaI G_GGCC'C
Cuts at: 0 114 121 162 262 1293
Size: 114 7 41 100 1031
Fragments arranged by size:
1031 114 100 41 7
ApaLI G'TGCA_C
Cuts at: 0 358 524 1293
Size: 358 166 769
Fragments arranged by size:
769 358 166
AvaI C'yCGr_G
Cuts at: 0 113 893 1293
Size: 113 780 400
Fragments arranged by size:
780 400 113
BanI G'GyrC_C
Cuts at: 0 237 929 1293
Size: 237 692 364
Fragments arranged by size:
692 364 237
BanII G_rGCy'C
Cuts at: 0 114 121 162 262 376 568 835 1293
Size: 114 7 41 100 114 192 267 458
Fragments arranged by size:
458 267 192 114 114 100 41 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 411 612 1293
Size: 411 201 681
Fragments arranged by size:
681 411 201
BclI T'GATC_A
Cuts at: 0 383 1293
Size: 383 910
BglI GCCn_nnn'nGGC
Cuts at: 0 769 778 1293
Size: 769 9 515
Fragments arranged by size:
769 515 9
BglII A'GATC_T
Cuts at: 0 144 1085 1293
Size: 144 941 208
Fragments arranged by size:
941 208 144
Bme1580I G_kGCm'C
Cuts at: 0 114 121 162 252 262 362 528 560
Size: 114 7 41 90 10 100 166 32
Cuts at: 560 690 1293
Size: 130 603
Fragments arranged by size:
603 166 130 114 100 90 41 32
10 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 422 515 1293
Size: 422 93 778
Fragments arranged by size:
778 422 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 271 388 685 745 763 1043 1072
Size: 96 175 117 297 60 18 280 29
Cuts at: 1072 1252 1293
Size: 180 41
Fragments arranged by size:
297 280 180 175 117 96 60 41
29 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 628 1293
Size: 412 216 665
Fragments arranged by size:
665 412 216
BsaWI w'CCGG_w
Cuts at: 0 923 1293
Size: 923 370
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 266 296 680 710 817 847 1293
Size: 266 30 384 30 107 30 446
Fragments arranged by size:
446 384 266 107 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 159 274 688 1293
Size: 159 115 414 605
Fragments arranged by size:
605 414 159 115
BseYI C'CCAG_C
Cuts at: 0 70 105 255 375 781 876 1040 1261
Size: 70 35 150 120 406 95 164 221
Cuts at: 1261 1293
Size: 32
Fragments arranged by size:
406 221 164 150 120 95 70 35
32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 489 1156 1293
Size: 68 421 667 137
Fragments arranged by size:
667 421 137 68
BsiHKAI G_wGCw'C
Cuts at: 0 362 528 568 1293
Size: 362 166 40 725
Fragments arranged by size:
725 362 166 40
Bsp1286I G_dGCh'C
Cuts at: 0 114 121 162 252 262 362 376 528
Size: 114 7 41 90 10 100 14 152
Cuts at: 528 560 568 690 835 1293
Size: 32 8 122 145 458
Fragments arranged by size:
458 152 145 122 114 100 90 41
32 14 10 8 7
BspEI T'CCGG_A
Cuts at: 0 923 1293
Size: 923 370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 345 1256 1293
Size: 110 235 911 37
Fragments arranged by size:
911 235 110 37
BsrFI r'CCGG_y
Cuts at: 0 492 1293
Size: 492 801
BstAPI GCAn_nnn'nTGC
Cuts at: 0 738 1293
Size: 738 555
BstBI TT'CG_AA
Cuts at: 0 623 1293
Size: 623 670
BstXI CCAn_nnnn'nTGG
Cuts at: 0 781 1293
Size: 781 512
BstYI r'GATC_y
Cuts at: 0 144 1085 1293
Size: 144 941 208
Fragments arranged by size:
941 208 144
BtgI C'CryG_G
Cuts at: 0 945 1220 1293
Size: 945 275 73
Fragments arranged by size:
945 275 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 182 1293
Size: 182 1111
BtsI GCAGTG_nn'
Cuts at: 0 217 496 574 648 1293
Size: 217 279 78 74 645
Fragments arranged by size:
645 279 217 78 74
EaeI y'GGCC_r
Cuts at: 0 464 1293
Size: 464 829
EarI CTCTTCn'nnn_
Cuts at: 0 177 620 1293
Size: 177 443 673
Fragments arranged by size:
673 443 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 673 1144 1293
Size: 673 471 149
Fragments arranged by size:
673 471 149
EcoICRI GAG'CTC
Cuts at: 0 566 1293
Size: 566 727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 271 388 685 745 763 1043 1072
Size: 96 175 117 297 60 18 280 29
Cuts at: 1072 1104 1252 1293
Size: 32 148 41
Fragments arranged by size:
297 280 175 148 117 96 60 41
32 29 18
EcoO109I rG'GnC_Cy
Cuts at: 0 110 117 158 259 865 900 1293
Size: 110 7 41 101 606 35 393
Fragments arranged by size:
606 393 110 101 41 35 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 817 849 1293
Size: 817 32 444
Fragments arranged by size:
817 444 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 1293
Size: 848 445
MscI TGG'CCA
Cuts at: 0 466 1293
Size: 466 827
MslI CAynn'nnrTG
Cuts at: 0 1105 1293
Size: 1105 188
MspA1I CmG'CkG
Cuts at: 0 74 204 210 735 1040 1071 1293
Size: 74 130 6 525 305 31 222
Fragments arranged by size:
525 305 222 130 74 31 6
NaeI GCC'GGC
Cuts at: 0 494 1293
Size: 494 799
NcoI C'CATG_G
Cuts at: 0 945 1293
Size: 945 348
NgoMIV G'CCGG_C
Cuts at: 0 492 1293
Size: 492 801
NspI r_CATG'y
Cuts at: 0 1008 1110 1293
Size: 1008 102 183
Fragments arranged by size:
1008 183 102
PasI CC'CwG_GG
Cuts at: 0 121 1293
Size: 121 1172
PflMI CCAn_nnn'nTGG
Cuts at: 0 669 981 1293
Size: 669 312 312
Fragments arranged by size:
669 312 312
PfoI T'CCnGG_A
Cuts at: 0 287 1049 1293
Size: 287 762 244
Fragments arranged by size:
762 287 244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1219 1251 1293
Size: 1219 32 42
Fragments arranged by size:
1219 42 32
PspOMI G'GGCC_C
Cuts at: 0 110 117 158 258 1293
Size: 110 7 41 100 1035
Fragments arranged by size:
1035 110 100 41 7
PstI C_TGCA'G
Cuts at: 0 209 356 1293
Size: 209 147 937
Fragments arranged by size:
937 209 147
PvuII CAG'CTG
Cuts at: 0 74 204 210 735 1293
Size: 74 130 6 525 558
Fragments arranged by size:
558 525 130 74 6
SacI G_AGCT'C
Cuts at: 0 568 1293
Size: 568 725
SapI GCTCTTCn'nnn_
Cuts at: 0 620 1293
Size: 620 673
SfcI C'TryA_G
Cuts at: 0 205 352 1293
Size: 205 147 941
Fragments arranged by size:
941 205 147
SmaI CCC'GGG
Cuts at: 0 115 895 1293
Size: 115 780 398
Fragments arranged by size:
780 398 115
SmlI C'TyrA_G
Cuts at: 0 427 643 1293
Size: 427 216 650
Fragments arranged by size:
650 427 216
StuI AGG'CCT
Cuts at: 0 15 1293
Size: 15 1278
StyI C'CwwG_G
Cuts at: 0 38 45 800 861 945 1293
Size: 38 7 755 61 84 348
Fragments arranged by size:
755 348 84 61 38 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 102 414 468 543 1293
Size: 102 312 54 75 750
Fragments arranged by size:
750 312 102 75 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 680 712 1293
Size: 680 32 581
Fragments arranged by size:
680 581 32
XmaI C'CCGG_G
Cuts at: 0 113 893 1293
Size: 113 780 400
Fragments arranged by size:
780 400 113
XmnI GAAnn'nnTTC
Cuts at: 0 619 1293
Size: 619 674
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BbsI BclI BglI BglII Bme1580I BmrI BpmI
BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspMI BsrFI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PflMI PfoI PpiI PspOMI PstI PvuII
SacI SapI SfcI SmaI SmlI StuI StyI TaqII
TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BlpI
BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrGI
BssHII BssSI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EagI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TatI Tth111I XbaI XcmI XhoI
ZraI