(Circular) (Six-base) MAPSORT of: pFN21AB7198.seq Check: 3914 from: 1 to: 6094 pFN21AB7198 6094 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2526 2526 Size: 6094 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3904 278 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3437 1937 Size: 1500 4594 Acc65I G'GTAC_C Cuts at: 2265 3415 2265 Size: 1150 4944 AclI AA'CG_TT Cuts at: 1563 4221 4594 1563 Size: 2658 373 3063 Fragments arranged by size: 3063 2658 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2343 2494 2627 2785 3029 4157 5541 5809 Size: 711 151 133 158 244 1128 1384 268 Cuts at: 5809 1632 Size: 1917 Fragments arranged by size: 1917 1384 1128 711 268 244 158 151 133 AfeI AGC'GCT Cuts at: 2147 3260 2147 Size: 1113 4981 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5897 AflIII A'CryG_T Cuts at: 1203 2301 4993 5970 1203 Size: 1098 2692 977 1327 Fragments arranged by size: 2692 1327 1098 977 AgeI A'CCGG_T Cuts at: 2307 4925 2307 Size: 2618 3476 AhdI GACnn_n'nnGTC Cuts at: 4823 4823 Size: 6094 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6094 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6094 AlwNI CAG_nnn'CTG Cuts at: 2428 3190 3837 5409 2428 Size: 762 647 1572 3113 Fragments arranged by size: 3113 1572 762 647 ApaLI G'TGCA_C Cuts at: 4151 5307 4151 Size: 1156 4938 ApoI r'AATT_y Cuts at: 1493 3239 3403 3629 3781 5890 1493 Size: 1746 164 226 152 2109 1697 Fragments arranged by size: 2109 1746 1697 226 164 152 AseI AT'TA_AT Cuts at: 160 1032 4648 160 Size: 872 3616 1606 Fragments arranged by size: 3616 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 AvaI C'yCGr_G Cuts at: 1870 1943 2048 3129 3419 1870 Size: 73 105 1081 290 4545 Fragments arranged by size: 4545 1081 290 105 73 BamHI G'GATC_C Cuts at: 2161 3424 3791 2161 Size: 1263 367 4464 Fragments arranged by size: 4464 1263 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 2084 2265 3415 4870 Size: 325 217 627 225 72 181 1150 1455 Cuts at: 4870 618 Size: 1842 Fragments arranged by size: 1842 1455 1150 627 325 225 217 181 72 BanII G_rGCy'C Cuts at: 1391 2655 3168 3413 1391 Size: 1264 513 245 4072 Fragments arranged by size: 4072 1264 513 245 BbeI G_GCGC'C Cuts at: 2016 2016 Size: 6094 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2578 928 Size: 971 679 4444 Fragments arranged by size: 4444 971 679 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2121 2155 4285 4319 1903 Size: 34 184 34 2130 34 3678 Fragments arranged by size: 3678 2130 184 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3010 3986 5196 3010 Size: 976 1210 3908 Fragments arranged by size: 3908 1210 976 BclI T'GATC_A Cuts at: 1548 1548 Size: 6094 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4705 136 Size: 107 122 71 631 605 3033 1525 Fragments arranged by size: 3033 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6089 6089 Size: 6094 BlpI GC'TnA_GC Cuts at: 3511 3511 Size: 6094 Bme1580I G_kGCm'C Cuts at: 1790 4155 5311 1790 Size: 2365 1156 2573 Fragments arranged by size: 2573 2365 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6094 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3825 4783 442 Size: 416 53 83 399 24 2408 958 1753 Fragments arranged by size: 2408 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6094 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2254 2254 Size: 6094 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2335 2383 4754 1239 Size: 1096 48 2371 2579 Fragments arranged by size: 2579 2371 1096 48 Bpu10I CC'TnA_GC Cuts at: 3332 3332 Size: 6094 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3290 3296 4219 5084 5382 3290 Size: 6 923 865 298 4002 Fragments arranged by size: 4002 923 865 298 6 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4757 882 Size: 617 3258 2219 Fragments arranged by size: 3258 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2302 2812 3885 493 Size: 711 1098 510 1073 2702 Fragments arranged by size: 2702 1098 1073 711 510 BsaBI GATnn'nnATC Cuts at: 6088 6088 Size: 6094 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2013 2134 3901 4283 Size: 53 83 186 936 480 121 1767 382 Cuts at: 4283 4918 275 Size: 635 1451 Fragments arranged by size: 1767 1451 936 635 480 382 186 121 83 53 BsaWI w'CCGG_w Cuts at: 2193 2307 4527 4925 5199 5346 5757 2193 Size: 114 2220 398 274 147 411 2530 Fragments arranged by size: 2530 2220 411 398 274 147 114 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2450 2480 2735 2765 1178 Size: 30 165 30 1047 30 255 30 4507 Fragments arranged by size: 4507 1047 255 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2399 2432 2492 2645 1186 Size: 1213 33 60 153 4635 Fragments arranged by size: 4635 1213 153 60 33 BseYI C'CCAG_C Cuts at: 2631 2823 3082 3252 5297 5793 2631 Size: 192 259 170 2045 496 2932 Fragments arranged by size: 2932 2045 496 259 192 170 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2132 2299 2504 2509 1163 Size: 573 396 167 205 5 4748 Fragments arranged by size: 4748 573 396 205 167 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3544 4305 5333 1540 Size: 275 186 1543 761 1028 2301 Fragments arranged by size: 2301 1543 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3126 3168 3276 4155 4240 5311 3126 Size: 42 108 879 85 1071 3909 Fragments arranged by size: 3909 1071 879 108 85 42 BsmI GAATG_Cn' Cuts at: 3605 3698 3605 Size: 93 6001 BsmBI CGTCTCn'nnnn_ Cuts at: 2099 2099 Size: 6094 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2655 3126 3168 3276 3413 4155 4240 Size: 399 865 471 42 108 137 742 85 Cuts at: 4240 5311 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 865 742 471 399 137 108 85 42 BspEI T'CCGG_A Cuts at: 5757 5757 Size: 6094 BspHI T'CATG_A Cuts at: 3091 3983 5713 3091 Size: 892 1730 3472 Fragments arranged by size: 3472 1730 892 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2277 2526 3449 844 Size: 1433 249 923 3489 Fragments arranged by size: 3489 1433 923 249 BsrBI CCG'CTC Cuts at: 1394 1823 3981 1394 Size: 429 2158 3507 Fragments arranged by size: 3507 2158 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2798 4589 4763 65 Size: 1183 1550 1791 174 1396 Fragments arranged by size: 1791 1550 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2066 2307 4738 4925 1700 Size: 110 199 57 241 2431 187 2869 Fragments arranged by size: 2869 2431 241 199 187 110 57 BsrGI T'GTAC_A Cuts at: 96 2757 3363 96 Size: 2661 606 2827 Fragments arranged by size: 2827 2661 606 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6094 BssSI C'ACGA_G Cuts at: 4154 5166 4154 Size: 1012 5082 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6094 BstBI TT'CG_AA Cuts at: 4898 4898 Size: 6094 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6094 BstXI CCAn_nnnn'nTGG Cuts at: 2648 2648 Size: 6094 BstYI r'GATC_y Cuts at: 1972 2161 2796 3424 3791 4176 4193 5645 5731 Size: 189 635 628 367 385 17 1452 86 Cuts at: 5731 5743 6089 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 635 628 385 367 346 189 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3345 513 Size: 552 939 1341 3262 Fragments arranged by size: 3262 1341 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5355 Fragments arranged by size: 5355 683 56 BtsI GCAGTG_nn' Cuts at: 2290 2521 2762 3343 3610 4404 4424 2290 Size: 231 241 581 267 794 20 3960 Fragments arranged by size: 3960 794 581 267 241 231 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6094 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6059 DraI TTT'AAA Cuts at: 3398 3398 Size: 6094 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5101 809 Size: 507 3785 1802 Fragments arranged by size: 3785 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2219 2718 Size: 54 1425 50 275 18 177 212 499 Cuts at: 2718 3198 3541 4430 6059 8 Size: 480 343 889 1629 43 Fragments arranged by size: 1629 1425 889 499 480 343 275 212 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3541 1537 Size: 275 1729 4090 Fragments arranged by size: 4090 1729 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3242 4024 1353 Size: 1889 782 3423 Fragments arranged by size: 3423 1889 782 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4681 5055 5201 387 Size: 1064 138 3092 374 146 1280 Fragments arranged by size: 3092 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3166 3166 Size: 6094 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2335 2343 2383 2494 2627 2785 3029 Size: 393 703 8 40 111 133 158 244 Cuts at: 3029 4157 4754 5541 5809 1239 Size: 1128 597 787 268 1524 Fragments arranged by size: 1524 1128 787 703 597 393 268 244 158 133 111 40 8 EcoNI CCTnn'n_nnAGG Cuts at: 2156 2876 2156 Size: 720 5374 EcoO109I rG'GnC_Cy Cuts at: 3135 3135 Size: 6094 EcoRI G'AATT_C Cuts at: 3403 3781 3403 Size: 378 5716 FseI GG_CCGG'CC Cuts at: 2070 2070 Size: 6094 FspI TGC'GCA Cuts at: 4600 4600 Size: 6094 HaeII r_GCGC'y Cuts at: 1678 2016 2149 3262 5241 1678 Size: 338 133 1113 1979 2531 Fragments arranged by size: 2531 1979 1113 338 133 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2146 2178 4735 4767 4809 Size: 32 135 32 574 32 2557 32 42 Cuts at: 4809 4841 1373 Size: 32 2626 Fragments arranged by size: 2626 2557 574 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3220 3438 3684 669 Size: 988 281 1282 218 246 3079 Fragments arranged by size: 3079 1282 988 281 246 218 HindIII A'AGCT_T Cuts at: 748 748 Size: 6094 HpaI GTT'AAC Cuts at: 3684 3684 Size: 6094 KasI G'GCGC_C Cuts at: 2012 2012 Size: 6094 KpnI G_GTAC'C Cuts at: 2269 3419 2269 Size: 1150 4944 MfeI C'AATT_G Cuts at: 3693 3693 Size: 6094 MluI A'CGCG_T Cuts at: 5970 5970 Size: 6094 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2355 2931 5208 5392 1305 Size: 1050 576 2277 184 2007 Fragments arranged by size: 2277 2007 1050 576 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2221 3200 10 Size: 54 1425 343 389 979 2904 Fragments arranged by size: 2904 1425 979 389 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2957 4052 4411 4570 518 Size: 648 1791 1095 359 159 2042 Fragments arranged by size: 2042 1791 1095 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2256 2321 3235 3510 4187 5335 5580 Size: 420 230 65 914 275 677 1148 245 Cuts at: 5580 1606 Size: 2120 Fragments arranged by size: 2120 1148 914 677 420 275 245 230 65 NaeI GCC'GGC Cuts at: 2011 2068 2011 Size: 57 6037 NarI GG'CG_CC Cuts at: 2013 2013 Size: 6094 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4603 Fragments arranged by size: 4603 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6094 NgoMIV G'CCGG_C Cuts at: 2009 2066 2009 Size: 57 6037 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6094 NotI GC'GGCC_GC Cuts at: 3541 3541 Size: 6094 NspI r_CATG'y Cuts at: 1132 1229 3364 3452 4997 1132 Size: 97 2135 88 1545 2229 Fragments arranged by size: 2229 2135 1545 97 88 PasI CC'CwG_GG Cuts at: 1348 2080 2274 2853 1348 Size: 732 194 579 4589 Fragments arranged by size: 4589 732 579 194 PciI A'CATG_T Cuts at: 4993 4993 Size: 6094 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6094 PmeI GTTT'AAAC Cuts at: 3398 3398 Size: 6094 PmlI CAC'GTG Cuts at: 2302 2302 Size: 6094 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4163 4195 5676 5708 4163 Size: 32 1481 32 4549 Fragments arranged by size: 4549 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3135 3135 Size: 6094 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6094 PsiI TTA'TAA Cuts at: 3664 3664 Size: 6094 PstI C_TGCA'G Cuts at: 830 2340 3392 3446 830 Size: 1510 1052 54 3478 Fragments arranged by size: 3478 1510 1052 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6094 PvuII CAG'CTG Cuts at: 2256 2321 3235 2256 Size: 65 914 5115 Fragments arranged by size: 5115 914 65 SacI G_AGCT'C Cuts at: 3168 3168 Size: 6094 SalI G'TCGA_C Cuts at: 1936 3436 1936 Size: 1500 4594 SanDI GG'GwC_CC Cuts at: 3135 3135 Size: 6094 SapI GCTCTTCn'nnn_ Cuts at: 3242 3242 Size: 6094 SbfI CC_TGCA'GG Cuts at: 3446 3446 Size: 6094 ScaI AGT'ACT Cuts at: 2708 4342 6054 2708 Size: 1634 1712 2748 Fragments arranged by size: 2748 1712 1634 SfcI C'TryA_G Cuts at: 826 1045 2336 3388 3442 4577 5258 5449 826 Size: 219 1291 1052 54 1135 681 191 1471 Fragments arranged by size: 1471 1291 1135 1052 681 219 191 54 SfoI GGC'GCC Cuts at: 2014 2014 Size: 6094 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6094 SmaI CCC'GGG Cuts at: 1872 3131 3421 1872 Size: 1259 290 4545 Fragments arranged by size: 4545 1259 290 SmlI C'TyrA_G Cuts at: 820 1017 1943 3269 3311 4198 5099 5361 820 Size: 197 926 1326 42 887 901 262 1553 Fragments arranged by size: 1553 1326 926 901 887 262 197 42 SnaBI TAC'GTA Cuts at: 493 3885 493 Size: 3392 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6094 SphI G_CATG'C Cuts at: 1132 3452 1132 Size: 2320 3774 SspI AAT'ATT Cuts at: 5 52 4018 5 Size: 47 3966 2081 Fragments arranged by size: 3966 2081 47 StuI AGG'CCT Cuts at: 1517 2328 3383 1517 Size: 811 1055 4228 Fragments arranged by size: 4228 1055 811 StyI C'CwwG_G Cuts at: 513 1065 2004 3533 513 Size: 552 939 1529 3074 Fragments arranged by size: 3074 1529 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2767 4121 4138 4291 4476 4921 325 Size: 1255 1187 1354 17 153 185 445 1498 Fragments arranged by size: 1498 1354 1255 1187 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2706 2757 3363 Size: 274 80 33 51 1443 729 51 606 Cuts at: 3363 4340 6052 96 Size: 977 1712 138 Fragments arranged by size: 1712 1443 977 729 606 274 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6054 6086 6054 Size: 32 6062 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6094 XbaI T'CTAG_A Cuts at: 3430 3430 Size: 6094 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6094 XmaI C'CCGG_G Cuts at: 1870 3129 3419 1870 Size: 1259 290 4545 Fragments arranged by size: 4545 1259 290 XmnI GAAnn'nnTTC Cuts at: 4223 4223 Size: 6094 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3902 276 Size: 53 83 186 936 2368 2468 Fragments arranged by size: 2468 2368 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI