Restriction map for
pFN21AB7198
(Linear) (Six-base) MAPSORT of: KIBB7198.seq Check: 9914 from: 1 to: 1389
KIBB7198.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 521 1389
Size: 521 868
Acc65I G'GTAC_C
Cuts at: 0 260 1389
Size: 260 1129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 489 622 780 1024 1389
Size: 338 151 133 158 244 365
Fragments arranged by size:
365 338 244 158 151 133
AfeI AGC'GCT
Cuts at: 0 142 1255 1389
Size: 142 1113 134
Fragments arranged by size:
1113 142 134
AflIII A'CryG_T
Cuts at: 0 296 1389
Size: 296 1093
AgeI A'CCGG_T
Cuts at: 0 302 1389
Size: 302 1087
AlwNI CAG_nnn'CTG
Cuts at: 0 423 1185 1389
Size: 423 762 204
Fragments arranged by size:
762 423 204
ApoI r'AATT_y
Cuts at: 0 1234 1389
Size: 1234 155
AvaI C'yCGr_G
Cuts at: 0 43 1124 1389
Size: 43 1081 265
Fragments arranged by size:
1081 265 43
BamHI G'GATC_C
Cuts at: 0 156 1389
Size: 156 1233
BanI G'GyrC_C
Cuts at: 0 7 79 260 1389
Size: 7 72 181 1129
Fragments arranged by size:
1129 181 72 7
BanII G_rGCy'C
Cuts at: 0 650 1163 1389
Size: 650 513 226
Fragments arranged by size:
650 513 226
BbeI G_GCGC'C
Cuts at: 0 11 1389
Size: 11 1378
BbsI GAAGACnn'nnnn_
Cuts at: 0 573 1389
Size: 573 816
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 116 150 1389
Size: 116 34 1239
Fragments arranged by size:
1239 116 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1005 1389
Size: 1005 384
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 249 1389
Size: 249 1140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 378 1389
Size: 330 48 1011
Fragments arranged by size:
1011 330 48
Bpu10I CC'TnA_GC
Cuts at: 0 1327 1389
Size: 1327 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1285 1291 1389
Size: 1285 6 98
Fragments arranged by size:
1285 98 6
BsaAI yAC'GTr
Cuts at: 0 297 807 1389
Size: 297 510 582
Fragments arranged by size:
582 510 297
BsaHI Gr'CG_yC
Cuts at: 0 8 129 1389
Size: 8 121 1260
Fragments arranged by size:
1260 121 8
BsaWI w'CCGG_w
Cuts at: 0 188 302 1389
Size: 188 114 1087
Fragments arranged by size:
1087 188 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 445 475 730 760 1389
Size: 445 30 255 30 629
Fragments arranged by size:
629 445 255 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 427 487 640 1389
Size: 394 33 60 153 749
Fragments arranged by size:
749 394 153 60 33
BseYI C'CCAG_C
Cuts at: 0 626 818 1077 1247 1389
Size: 626 192 259 170 142
Fragments arranged by size:
626 259 192 170 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 294 499 504 1389
Size: 127 167 205 5 885
Fragments arranged by size:
885 205 167 127 5
BsiHKAI G_wGCw'C
Cuts at: 0 1121 1163 1271 1389
Size: 1121 42 108 118
Fragments arranged by size:
1121 118 108 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 94 1389
Size: 94 1295
Bsp1286I G_dGCh'C
Cuts at: 0 650 1121 1163 1271 1389
Size: 650 471 42 108 118
Fragments arranged by size:
650 471 118 108 42
BspHI T'CATG_A
Cuts at: 0 1086 1389
Size: 1086 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 272 521 1389
Size: 272 249 868
Fragments arranged by size:
868 272 249
BsrDI GCAATG_nn'
Cuts at: 0 793 1389
Size: 793 596
BsrFI r'CCGG_y
Cuts at: 0 4 61 302 1389
Size: 4 57 241 1087
Fragments arranged by size:
1087 241 57 4
BsrGI T'GTAC_A
Cuts at: 0 752 1358 1389
Size: 752 606 31
Fragments arranged by size:
752 606 31
BstXI CCAn_nnnn'nTGG
Cuts at: 0 643 1389
Size: 643 746
BstYI r'GATC_y
Cuts at: 0 156 791 1389
Size: 156 635 598
Fragments arranged by size:
635 598 156
BtgI C'CryG_G
Cuts at: 0 1340 1389
Size: 1340 49
BtsI GCAGTG_nn'
Cuts at: 0 285 516 757 1338 1389
Size: 285 231 241 581 51
Fragments arranged by size:
581 285 241 231 51
EaeI y'GGCC_r
Cuts at: 0 2 214 713 1193 1389
Size: 2 212 499 480 196
Fragments arranged by size:
499 480 212 196 2
EarI CTCTTCn'nnn_
Cuts at: 0 1237 1389
Size: 1237 152
EcoICRI GAG'CTC
Cuts at: 0 1161 1389
Size: 1161 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 338 378 489 622 780 1024 1389
Size: 330 8 40 111 133 158 244 365
Fragments arranged by size:
365 330 244 158 133 111 40 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 151 871 1389
Size: 151 720 518
Fragments arranged by size:
720 518 151
EcoO109I rG'GnC_Cy
Cuts at: 0 1130 1389
Size: 1130 259
FseI GG_CCGG'CC
Cuts at: 0 65 1389
Size: 65 1324
HaeII r_GCGC'y
Cuts at: 0 11 144 1257 1389
Size: 11 133 1113 132
Fragments arranged by size:
1113 133 132 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 141 173 1389
Size: 141 32 1216
Fragments arranged by size:
1216 141 32
HincII GTy'rAC
Cuts at: 0 1215 1389
Size: 1215 174
KasI G'GCGC_C
Cuts at: 0 7 1389
Size: 7 1382
KpnI G_GTAC'C
Cuts at: 0 264 1389
Size: 264 1125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 926 1389
Size: 350 576 463
Fragments arranged by size:
576 463 350
MscI TGG'CCA
Cuts at: 0 216 1195 1389
Size: 216 979 194
Fragments arranged by size:
979 216 194
MslI CAynn'nnrTG
Cuts at: 0 952 1389
Size: 952 437
MspA1I CmG'CkG
Cuts at: 0 21 251 316 1230 1389
Size: 21 230 65 914 159
Fragments arranged by size:
914 230 159 65 21
NaeI GCC'GGC
Cuts at: 0 6 63 1389
Size: 6 57 1326
Fragments arranged by size:
1326 57 6
NarI GG'CG_CC
Cuts at: 0 8 1389
Size: 8 1381
NgoMIV G'CCGG_C
Cuts at: 0 4 61 1389
Size: 4 57 1328
Fragments arranged by size:
1328 57 4
NspI r_CATG'y
Cuts at: 0 1359 1389
Size: 1359 30
PasI CC'CwG_GG
Cuts at: 0 75 269 848 1389
Size: 75 194 579 541
Fragments arranged by size:
579 541 194 75
PmlI CAC'GTG
Cuts at: 0 297 1389
Size: 297 1092
PpuMI rG'GwC_Cy
Cuts at: 0 1130 1389
Size: 1130 259
PstI C_TGCA'G
Cuts at: 0 335 1387 1389
Size: 335 1052 2
Fragments arranged by size:
1052 335 2
PvuII CAG'CTG
Cuts at: 0 251 316 1230 1389
Size: 251 65 914 159
Fragments arranged by size:
914 251 159 65
SacI G_AGCT'C
Cuts at: 0 1163 1389
Size: 1163 226
SanDI GG'GwC_CC
Cuts at: 0 1130 1389
Size: 1130 259
SapI GCTCTTCn'nnn_
Cuts at: 0 1237 1389
Size: 1237 152
ScaI AGT'ACT
Cuts at: 0 703 1389
Size: 703 686
SfcI C'TryA_G
Cuts at: 0 331 1383 1389
Size: 331 1052 6
Fragments arranged by size:
1052 331 6
SfoI GGC'GCC
Cuts at: 0 9 1389
Size: 9 1380
SmaI CCC'GGG
Cuts at: 0 1126 1389
Size: 1126 263
SmlI C'TyrA_G
Cuts at: 0 1264 1306 1389
Size: 1264 42 83
Fragments arranged by size:
1264 83 42
StuI AGG'CCT
Cuts at: 0 323 1378 1389
Size: 323 1055 11
Fragments arranged by size:
1055 323 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 762 1389
Size: 762 627
TatI w'GTAC_w
Cuts at: 0 701 752 1358 1389
Size: 701 51 606 31
Fragments arranged by size:
701 606 51 31
XmaI C'CCGG_G
Cuts at: 0 1124 1389
Size: 1124 265
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AgeI AlwNI ApoI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrGI BstXI BstYI BtgI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PmlI PpuMI PstI PvuII
SacI SanDI SapI ScaI SfcI SfoI SmaI SmlI
StuI TaqII TatI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AhdI AleI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BsaI BsaBI BsiEI BsiWI BsmI BspEI BsrBI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI
EcoRV FalI FspI FspAI HindIII HpaI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SbfI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StyI
SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI