Restriction map for
pFN21AB7197
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaI BseAI BseRI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssSI Bst1107I
BstBI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI Eam1105I EcoNI
EcoRI EcoRV EcoT22I FseI HindIII
HinfI HpaI KpnI KspI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AocI 527 CCTCAGG CC^T[ACGT]AGG
AvaI 572 CCCGGG C^[CT]CG[GA]G
AviII 719 TGCGCA TGC^GCA
BalI 290 TGGCCA TGG^CCA
BanII 741 GGGCTC G[GA]GC[CT]^C
BbsI 240 GAAGAC GAAGAC(2/6)
BclI 500 TGATCA T^GATCA
BfaI 71 CTAG C^TAG
BglII 641 AGATCT A^GATCT
BpmI 598 CTCCAG CTGGAG(16/14)
BpuAI 240 GAAGAC GAAGAC(2/6)
BsaBI 501 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 224 TCCGGT [AT]^CCGG[AT]
BsmAI 334 GAGAC GTCTC(1/5)
BsmBI 334 GAGACG CGTCTC(1/5)
BsoBI 572 CCCGGG C^[CT]CG[GA]G
BsrBI 40 CCGCTC CCGCTC(-3/-3)
BssHII 116 GCGCGC G^CGCGC
BstAPI 1001 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 633 GGTGACC G^GT[ACGT]ACC
BstPI 633 GGTGACC G^GT[ACGT]ACC
BstYI 641 AGATCT [GA]^GATC[CT]
Bsu36I 527 CCTCAGG CC^T[ACGT]AGG
DraIII 687 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 527 CCTCAGG CC^T[ACGT]AGG
EcoO65I 633 GGTGACC G^GT[ACGT]ACC
FbaI 500 TGATCA T^GATCA
FspI 719 TGCGCA TGC^GCA
HincII 430 GTTGAC GT[CT]^[GA]AC
HindII 430 GTTGAC GT[CT]^[GA]AC
MaeI 71 CTAG C^TAG
MamI 501 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 641 AGATCT [GA]^GATC[CT]
MluNI 290 TGGCCA TGG^CCA
MscI 290 TGGCCA TGG^CCA
NaeI 19 GCCGGC GCC^GGC
NgoMIV 19 GCCGGC G^CCGGC
NotI 1010 GCGGCCGC GC^GGCCGC
NspI 593 GCATGC [GA]CATG^[CT]
PpuMI 518 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 31 CTGCAG CTGCA^G
PvuII 615 CAGCTG CAG^CTG
SapI 42 GCTCTTC GCTCTTC(1/4)
SfcI 31 CTGCAG C^T[GA][CT]AG
SgrAI 18 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 572 CCCGGG CCC^GGG
SphI 593 GCATGC GCATG^C
XhoII 641 AGATCT [GA]^GATC[CT]
XmaCI 572 CCCGGG C^CCGGG
XmaI 572 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Aor51HI 85 AGCGCT AGC^GCT
977 AGCGCT
AspHI 370 GTGCTC G[AT]GC[AT]^C
946 GTGCTC
BsgI 898 CTGCAC GTGCAG(16/14)
1077 CTGCAC
BsiHKAI 370 GTGCTC G[AT]GC[AT]^C
946 GTGCTC
BsrI 623 ACTGG ACTGG(1/-1)
600 CCAGT
BstF5I 675 CATCC GGATG(2/0)
828 CATCC
DpnII 501 GATC ^GATC
642 GATC
DraII 464 AGGCCCT [GA]G^G[ACGT]CC[CT]
518 GGGTCCT
DsaI 182 CCGTGG C^C[GA][CT]GG
1052 CCACGG
EagI 170 CGGCCG C^GGCCG
1011 CGGCCG
EarI 43 CTCTTC CTCTTC(1/4)
655 CTCTTC
EclXI 170 CGGCCG C^GGCCG
1011 CGGCCG
Eco47III 85 AGCGCT AGC^GCT
977 AGCGCT
Eco52I 170 CGGCCG C^GGCCG
1011 CGGCCG
EcoO109I 464 AGGCCCT [GA]G^G[ACGT]CC[CT]
518 GGGTCCT
EcoT14I 488 CCAAGG C^C[AT][AT]GG
746 CCAAGG
FokI 675 CATCC GGATG(9/13)
828 CATCC
Ksp632I 43 CTCTTC CTCTTC(1/4)
655 CTCTTC
MaeII 200 ACGT A^CGT
337 ACGT
MboI 501 GATC ^GATC
642 GATC
MspA1I 615 CAGCTG C[AC]G^C[GT]G
880 CCGCTG
NdeII 501 GATC ^GATC
642 GATC
Sau3AI 501 GATC ^GATC
642 GATC
StyI 488 CCAAGG C^C[AT][AT]GG
746 CCAAGG
TspRI 584 CAGTG CA[GC]TG[ACGT][ACGT]^
723 CAGTG
XcmI 320 CCAAGTACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
545 CCATCATGGTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BanI BbeI BbvI BcnI
BmyI BsaHI BsaJI BsiEI BsiYI
BslI BsmFI Bsp1286I BsrFI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI EaeI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI KasI MaeIII MboII MnlI
MslI MspI MvaI MvnI MwoI
NarI NciI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI SfoI TaqI
TseI Tsp45I TthHB8I