(Circular) (Six-base) MAPSORT of: pFN21AB7197.seq Check: 8149 from: 1 to: 5821 pFN21AB7197 5821 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3164 1937 Size: 1227 4594 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3022 3884 5268 5536 1632 Size: 1390 862 1384 268 1917 Fragments arranged by size: 1917 1390 1384 862 268 AfeI AGC'GCT Cuts at: 2092 2984 2092 Size: 892 4929 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 4720 5697 1203 Size: 3517 977 1327 Fragments arranged by size: 3517 1327 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2390 2611 1184 Size: 1206 221 4394 Fragments arranged by size: 4394 1206 221 AlwNI CAG_nnn'CTG Cuts at: 3564 5136 3564 Size: 1572 4249 ApaLI G'TGCA_C Cuts at: 3878 5034 3878 Size: 1156 4665 ApoI r'AATT_y Cuts at: 1493 3130 3356 3508 5617 1493 Size: 1637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1637 226 152 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 2577 3146 1870 Size: 73 634 569 4545 Fragments arranged by size: 4545 634 569 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2500 2533 2500 Size: 33 5788 BamHI G'GATC_C Cuts at: 3151 3518 3151 Size: 367 5454 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2027 2096 2674 2807 2827 Size: 325 217 627 240 69 578 133 20 Cuts at: 2827 3142 4597 618 Size: 315 1455 1842 Fragments arranged by size: 1842 1455 627 578 325 315 240 217 133 69 20 BanII G_rGCy'C Cuts at: 1391 2750 3140 1391 Size: 1359 390 4072 Fragments arranged by size: 4072 1359 390 BbeI G_GCGC'C Cuts at: 2031 2100 2811 2831 2031 Size: 69 711 20 5021 Fragments arranged by size: 5021 711 69 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2252 928 Size: 971 353 4497 Fragments arranged by size: 4497 971 353 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4012 4046 1903 Size: 34 2075 34 3678 Fragments arranged by size: 3678 2075 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4923 3713 Size: 1210 4611 BclI T'GATC_A Cuts at: 1548 2505 1548 Size: 957 4864 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4432 136 Size: 107 122 71 631 605 2760 1525 Fragments arranged by size: 2760 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2646 5816 2646 Size: 3170 2651 BlpI GC'TnA_GC Cuts at: 3238 3238 Size: 5821 Bme1580I G_kGCm'C Cuts at: 1790 2550 2679 3882 5038 1790 Size: 760 129 1203 1156 2573 Fragments arranged by size: 2573 1203 1156 760 129 BmgBI CAC'GTC Cuts at: 1318 2343 1318 Size: 1025 4796 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2638 3552 4510 Size: 416 53 83 399 24 1221 914 958 Cuts at: 4510 442 Size: 1753 Fragments arranged by size: 1753 1221 958 914 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2588 4481 1239 Size: 1349 1893 2579 Fragments arranged by size: 2579 1893 1349 Bpu10I CC'TnA_GC Cuts at: 2281 2281 Size: 5821 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3946 4811 5109 3946 Size: 865 298 4658 Fragments arranged by size: 4658 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4484 882 Size: 617 2985 2219 Fragments arranged by size: 2985 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3612 493 Size: 711 2408 2702 Fragments arranged by size: 2702 2408 711 BsaBI GATnn'nnATC Cuts at: 2510 5815 2510 Size: 3305 2516 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2028 2067 2097 2808 Size: 53 83 186 936 495 39 30 711 Cuts at: 2808 2828 3628 4010 4645 275 Size: 20 800 382 635 1451 Fragments arranged by size: 1451 936 800 711 635 495 382 186 83 53 39 30 20 BsaWI w'CCGG_w Cuts at: 2229 4254 4652 4926 5073 5484 2229 Size: 2025 398 274 147 411 2566 Fragments arranged by size: 2566 2025 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5596 Fragments arranged by size: 5596 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5821 BseYI C'CCAG_C Cuts at: 2087 2197 2612 2618 2920 3029 5024 5520 2087 Size: 110 415 6 302 109 1995 496 2388 Fragments arranged by size: 2388 1995 496 415 302 110 109 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2888 3067 1163 Size: 573 1152 179 3917 Fragments arranged by size: 3917 1152 573 179 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2178 2875 3019 3271 4032 5060 Size: 275 186 177 697 144 252 761 1028 Cuts at: 5060 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 697 275 252 186 177 144 BsiHKAI G_wGCw'C Cuts at: 2379 2955 3882 3967 5038 2379 Size: 576 927 85 1071 3162 Fragments arranged by size: 3162 1071 927 576 85 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 BsmBI CGTCTCn'nnnn_ Cuts at: 2333 2333 Size: 5821 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2379 2550 2679 2750 2955 3140 3882 Size: 399 589 171 129 71 205 185 742 Cuts at: 3882 3967 5038 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 589 399 205 185 171 129 85 71 BspEI T'CCGG_A Cuts at: 5484 5484 Size: 5821 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3176 844 Size: 2332 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2047 3708 1394 Size: 429 224 1661 3507 Fragments arranged by size: 3507 1661 429 224 BsrDI GCAATG_nn' Cuts at: 65 1248 4316 4490 65 Size: 1183 3068 174 1396 Fragments arranged by size: 3068 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2011 2024 2874 4465 4652 1700 Size: 110 201 13 850 1591 187 2869 Fragments arranged by size: 2869 1591 850 201 187 110 13 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 2121 1925 Size: 196 5625 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 3012 794 Size: 2218 3603 BstBI TT'CG_AA Cuts at: 4625 4625 Size: 5821 BstEII G'GTnAC_C Cuts at: 1184 2638 1184 Size: 1454 4367 BstYI r'GATC_y Cuts at: 1972 2646 3151 3518 3903 3920 5372 5458 5470 Size: 674 505 367 385 17 1452 86 12 Cuts at: 5470 5816 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 674 505 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2533 2533 Size: 5821 BtgI C'CryG_G Cuts at: 513 1065 2004 2187 3057 513 Size: 552 939 183 870 3277 Fragments arranged by size: 3277 939 870 552 183 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2431 2899 487 Size: 683 56 1205 468 3409 Fragments arranged by size: 3409 1205 683 468 56 BtsI GCAGTG_nn' Cuts at: 2595 2734 3337 4131 4151 2595 Size: 139 603 794 20 4265 Fragments arranged by size: 4265 794 603 139 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DraIII CAC_nnn'GTG Cuts at: 2697 2697 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4828 809 Size: 507 3512 1802 Fragments arranged by size: 3512 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2250 2295 Size: 54 1425 50 275 18 345 75 45 Cuts at: 2295 3016 3268 4157 5786 8 Size: 721 252 889 1629 43 Fragments arranged by size: 1629 1425 889 721 345 275 252 75 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2175 3016 3268 1537 Size: 275 363 841 252 4090 Fragments arranged by size: 4090 841 363 275 252 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2666 3751 1353 Size: 701 612 1085 3423 Fragments arranged by size: 3423 1085 701 612 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2011 2981 4408 4782 4928 387 Size: 1064 138 422 970 1427 374 146 1280 Fragments arranged by size: 1427 1280 1064 970 422 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2588 3022 3884 4481 5268 5536 1239 Size: 393 956 434 862 597 787 268 1524 Fragments arranged by size: 1524 956 862 787 597 434 393 268 EcoO109I rG'GnC_Cy Cuts at: 2470 2524 2470 Size: 54 5767 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3004 3004 Size: 5821 FspI TGC'GCA Cuts at: 2726 4327 2726 Size: 1601 4220 HaeII r_GCGC'y Cuts at: 1678 2031 2082 2094 2100 2133 2811 2831 2986 Size: 353 51 12 6 33 678 20 155 Cuts at: 2986 3114 4968 1678 Size: 128 1854 2531 Fragments arranged by size: 2531 1854 678 353 155 128 51 33 20 12 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4462 4494 4536 4568 1373 Size: 32 135 32 2890 32 42 32 2626 Fragments arranged by size: 2890 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2437 3165 3411 669 Size: 988 281 499 728 246 3079 Fragments arranged by size: 3079 988 728 499 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5821 HpaI GTT'AAC Cuts at: 3411 3411 Size: 5821 KasI G'GCGC_C Cuts at: 2027 2096 2807 2827 2027 Size: 69 711 20 5021 Fragments arranged by size: 5021 711 69 20 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4935 5119 1305 Size: 3630 184 2007 Fragments arranged by size: 3630 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 10 Size: 54 1425 343 465 3534 Fragments arranged by size: 3534 1425 465 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2240 2426 2558 2564 2693 2786 3779 Size: 648 1074 186 132 6 129 93 993 Cuts at: 3779 4138 4297 518 Size: 359 159 2042 Fragments arranged by size: 2042 1074 993 648 359 186 159 132 129 93 6 MspA1I CmG'CkG Cuts at: 1606 2622 2887 3237 3914 5062 5307 1606 Size: 1016 265 350 677 1148 245 2120 Fragments arranged by size: 2120 1148 1016 677 350 265 245 NaeI GCC'GGC Cuts at: 2026 2026 Size: 5821 NarI GG'CG_CC Cuts at: 2028 2097 2808 2828 2028 Size: 69 711 20 5021 Fragments arranged by size: 5021 711 69 20 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4330 Fragments arranged by size: 4330 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NgoMIV G'CCGG_C Cuts at: 2024 2024 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3016 3268 3016 Size: 252 5569 NspI r_CATG'y Cuts at: 1132 1229 2602 3179 4724 1132 Size: 97 1373 577 1545 2229 Fragments arranged by size: 2229 1545 1373 577 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 4720 4720 Size: 5821 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5821 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2524 2524 Size: 5821 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PstI C_TGCA'G Cuts at: 830 2040 3173 830 Size: 1210 1133 3478 Fragments arranged by size: 3478 1210 1133 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 PvuII CAG'CTG Cuts at: 2622 2622 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SapI GCTCTTCn'nnn_ Cuts at: 2054 2054 Size: 5821 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 4069 5781 4069 Size: 1712 4109 SfcI C'TryA_G Cuts at: 826 1045 2036 3169 4304 4985 5176 826 Size: 219 991 1133 1135 681 191 1471 Fragments arranged by size: 1471 1135 1133 991 681 219 191 SfoI GGC'GCC Cuts at: 2029 2098 2809 2829 2029 Size: 69 711 20 5021 Fragments arranged by size: 5021 711 69 20 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 2024 1700 Size: 324 5497 SmaI CCC'GGG Cuts at: 1872 2579 3148 1872 Size: 707 569 4545 Fragments arranged by size: 4545 707 569 SmlI C'TyrA_G Cuts at: 820 1017 1943 3925 4826 5088 820 Size: 197 926 1982 901 262 1553 Fragments arranged by size: 1982 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3612 493 Size: 3119 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 2602 3179 1132 Size: 1470 577 3774 Fragments arranged by size: 3774 1470 577 SspI AAT'ATT Cuts at: 5 52 3745 5 Size: 47 3693 2081 Fragments arranged by size: 3693 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5821 StyI C'CwwG_G Cuts at: 513 1065 2004 2493 2751 3260 513 Size: 552 939 489 258 509 3074 Fragments arranged by size: 3074 939 552 509 489 258 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3848 3865 4018 4203 4648 325 Size: 1255 2268 17 153 185 445 1498 Fragments arranged by size: 2268 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4067 5779 96 Size: 274 80 33 51 1443 2090 1712 138 Fragments arranged by size: 2090 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5781 5813 5781 Size: 32 5789 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5821 XbaI T'CTAG_A Cuts at: 3157 3157 Size: 5821 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2332 2557 2332 Size: 225 5596 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 2577 3146 1870 Size: 707 569 4545 Fragments arranged by size: 4545 707 569 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI