Restriction map for pFN21AB7197
 (Circular) (Six-base) MAPSORT of: pFN21AB7197.seq  Check: 8149  from: 1  to: 5821



pFN21AB7197  5821 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:38  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3631     278 
   Size:         53      83     186     936    2095    2468

  Fragments arranged by size: 

                2468    2095     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3164    1937 
   Size:       1227    4594
 
Acc65I G'GTAC_C

Cuts at:   3142    3142 
   Size:       5821
 
AclI AA'CG_TT

Cuts at:   1563    3948    4321    1563 
   Size:       2385     373    3063

  Fragments arranged by size: 

                3063    2385     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3022    3884    5268    5536    1632 
   Size:       1390     862    1384     268    1917

  Fragments arranged by size: 

                1917    1390    1384     862     268
 
AfeI AGC'GCT

Cuts at:   2092    2984    2092 
   Size:        892    4929
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5624
 
AflIII A'CryG_T

Cuts at:   1203    4720    5697    1203 
   Size:       3517     977    1327

  Fragments arranged by size: 

                3517    1327     977
 
AgeI A'CCGG_T

Cuts at:   4652    4652 
   Size:       5821
 
AhdI GACnn_n'nnGTC

Cuts at:   4550    4550 
   Size:       5821
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5821
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2390    2611    1184 
   Size:       1206     221    4394

  Fragments arranged by size: 

                4394    1206     221
 
AlwNI CAG_nnn'CTG

Cuts at:   3564    5136    3564 
   Size:       1572    4249
 
ApaLI G'TGCA_C

Cuts at:   3878    5034    3878 
   Size:       1156    4665
 
ApoI r'AATT_y

Cuts at:   1493    3130    3356    3508    5617    1493 
   Size:       1637     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1637     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4375     160 
   Size:        872    3343    1606

  Fragments arranged by size: 

                3343    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5821
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2577    3146    1870 
   Size:         73     634     569    4545

  Fragments arranged by size: 

                4545     634     569      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2500    2533    2500 
   Size:         33    5788
 
BamHI G'GATC_C

Cuts at:   3151    3518    3151 
   Size:        367    5454
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2027    2096    2674    2807    2827 
   Size:        325     217     627     240      69     578     133      20

Cuts at:   2827    3142    4597     618 
   Size:        315    1455    1842

  Fragments arranged by size: 

                1842    1455     627     578     325     315     240     217
                 133      69      20
 
BanII G_rGCy'C

Cuts at:   1391    2750    3140    1391 
   Size:       1359     390    4072

  Fragments arranged by size: 

                4072    1359     390
 
BbeI G_GCGC'C

Cuts at:   2031    2100    2811    2831    2031 
   Size:         69     711      20    5021

  Fragments arranged by size: 

                5021     711      69      20
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2252     928 
   Size:        971     353    4497

  Fragments arranged by size: 

                4497     971     353
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4012    4046    1903 
   Size:         34    2075      34    3678

  Fragments arranged by size: 

                3678    2075      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3713    4923    3713 
   Size:       1210    4611
 
BclI T'GATC_A

Cuts at:   1548    2505    1548 
   Size:        957    4864
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4432     136 
   Size:        107     122      71     631     605    2760    1525

  Fragments arranged by size: 

                2760    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2646    5816    2646 
   Size:       3170    2651
 
BlpI GC'TnA_GC

Cuts at:   3238    3238 
   Size:       5821
 
Bme1580I G_kGCm'C

Cuts at:   1790    2550    2679    3882    5038    1790 
   Size:        760     129    1203    1156    2573

  Fragments arranged by size: 

                2573    1203    1156     760     129
 
BmgBI CAC'GTC

Cuts at:   1318    2343    1318 
   Size:       1025    4796
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2638    3552    4510 
   Size:        416      53      83     399      24    1221     914     958

Cuts at:   4510     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1221     958     914     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5821
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2588    4481    1239 
   Size:       1349    1893    2579

  Fragments arranged by size: 

                2579    1893    1349
 
Bpu10I CC'TnA_GC

Cuts at:   2281    2281 
   Size:       5821
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3946    4811    5109    3946 
   Size:        865     298    4658

  Fragments arranged by size: 

                4658     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4484     882 
   Size:        617    2985    2219

  Fragments arranged by size: 

                2985    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3612     493 
   Size:        711    2408    2702

  Fragments arranged by size: 

                2702    2408     711
 
BsaBI GATnn'nnATC

Cuts at:   2510    5815    2510 
   Size:       3305    2516
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2028    2067    2097    2808 
   Size:         53      83     186     936     495      39      30     711

Cuts at:   2808    2828    3628    4010    4645     275 
   Size:         20     800     382     635    1451

  Fragments arranged by size: 

                1451     936     800     711     635     495     382     186
                  83      53      39      30      20
 
BsaWI w'CCGG_w

Cuts at:   2229    4254    4652    4926    5073    5484    2229 
   Size:       2025     398     274     147     411    2566

  Fragments arranged by size: 

                2566    2025     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5596

  Fragments arranged by size: 

                5596     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5821
 
BseYI C'CCAG_C

Cuts at:   2087    2197    2612    2618    2920    3029    5024    5520    2087 
   Size:        110     415       6     302     109    1995     496    2388

  Fragments arranged by size: 

                2388    1995     496     415     302     110     109       6
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2888    3067    1163 
   Size:        573    1152     179    3917

  Fragments arranged by size: 

                3917    1152     573     179
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2178    2875    3019    3271    4032    5060 
   Size:        275     186     177     697     144     252     761    1028

Cuts at:   5060    1540 
   Size:       2301

  Fragments arranged by size: 

                2301    1028     761     697     275     252     186     177
                 144
 
BsiHKAI G_wGCw'C

Cuts at:   2379    2955    3882    3967    5038    2379 
   Size:        576     927      85    1071    3162

  Fragments arranged by size: 

                3162    1071     927     576      85
 
BsmI GAATG_Cn'

Cuts at:   3332    3425    3332 
   Size:         93    5728
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2333    2333 
   Size:       5821
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2379    2550    2679    2750    2955    3140    3882 
   Size:        399     589     171     129      71     205     185     742

Cuts at:   3882    3967    5038    1391 
   Size:         85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     589     399     205     185     171
                 129      85      71
 
BspEI T'CCGG_A

Cuts at:   5484    5484 
   Size:       5821
 
BspHI T'CATG_A

Cuts at:   3710    5440    3710 
   Size:       1730    4091
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3176     844 
   Size:       2332    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2047    3708    1394 
   Size:        429     224    1661    3507

  Fragments arranged by size: 

                3507    1661     429     224
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4316    4490      65 
   Size:       1183    3068     174    1396

  Fragments arranged by size: 

                3068    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2011    2024    2874    4465    4652    1700 
   Size:        110     201      13     850    1591     187    2869

  Fragments arranged by size: 

                2869    1591     850     201     187     110      13
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5821
 
BssHII G'CGCG_C

Cuts at:   1925    2121    1925 
   Size:        196    5625
 
BssSI C'ACGA_G

Cuts at:   3881    4893    3881 
   Size:       1012    4809
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3012     794 
   Size:       2218    3603
 
BstBI TT'CG_AA

Cuts at:   4625    4625 
   Size:       5821
 
BstEII G'GTnAC_C

Cuts at:   1184    2638    1184 
   Size:       1454    4367
 
BstYI r'GATC_y

Cuts at:   1972    2646    3151    3518    3903    3920    5372    5458    5470 
   Size:        674     505     367     385      17    1452      86      12

Cuts at:   5470    5816    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     674     505     385     367     346      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2533    2533 
   Size:       5821
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2187    3057     513 
   Size:        552     939     183     870    3277

  Fragments arranged by size: 

                3277     939     870     552     183
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2431    2899     487 
   Size:        683      56    1205     468    3409

  Fragments arranged by size: 

                3409    1205     683     468      56
 
BtsI GCAGTG_nn'

Cuts at:   2595    2734    3337    4131    4151    2595 
   Size:        139     603     794      20    4265

  Fragments arranged by size: 

                4265     794     603     139      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5821
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5786
 
DraI TTT'AAA

Cuts at:   3125    3125 
   Size:       5821
 
DraIII CAC_nnn'GTG

Cuts at:   2697    2697 
   Size:       5821
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4828     809 
   Size:        507    3512    1802

  Fragments arranged by size: 

                3512    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2175    2250    2295 
   Size:         54    1425      50     275      18     345      75      45

Cuts at:   2295    3016    3268    4157    5786       8 
   Size:        721     252     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     721     345     275     252      75
                  54      50      45      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2175    3016    3268    1537 
   Size:        275     363     841     252    4090

  Fragments arranged by size: 

                4090     841     363     275     252
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2054    2666    3751    1353 
   Size:        701     612    1085    3423

  Fragments arranged by size: 

                3423    1085     701     612
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2011    2981    4408    4782    4928     387 
   Size:       1064     138     422     970    1427     374     146    1280

  Fragments arranged by size: 

                1427    1280    1064     970     422     374     146     138
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2588    3022    3884    4481    5268    5536    1239 
   Size:        393     956     434     862     597     787     268    1524

  Fragments arranged by size: 

                1524     956     862     787     597     434     393     268
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2470    2524    2470 
   Size:         54    5767
 
EcoRI G'AATT_C

Cuts at:   3130    3508    3130 
   Size:        378    5443
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3004    3004 
   Size:       5821
 
FspI TGC'GCA

Cuts at:   2726    4327    2726 
   Size:       1601    4220
 
HaeII r_GCGC'y

Cuts at:   1678    2031    2082    2094    2100    2133    2811    2831    2986 
   Size:        353      51      12       6      33     678      20     155

Cuts at:   2986    3114    4968    1678 
   Size:        128    1854    2531

  Fragments arranged by size: 

                2531    1854     678     353     155     128      51      33
                  20      12       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4462    4494    4536    4568    1373 
   Size:         32     135      32    2890      32      42      32    2626

  Fragments arranged by size: 

                2890    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2437    3165    3411     669 
   Size:        988     281     499     728     246    3079

  Fragments arranged by size: 

                3079     988     728     499     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5821
 
HpaI GTT'AAC

Cuts at:   3411    3411 
   Size:       5821
 
KasI G'GCGC_C

Cuts at:   2027    2096    2807    2827    2027 
   Size:         69     711      20    5021

  Fragments arranged by size: 

                5021     711      69      20
 
KpnI G_GTAC'C

Cuts at:   3146    3146 
   Size:       5821
 
MfeI C'AATT_G

Cuts at:   3420    3420 
   Size:       5821
 
MluI A'CGCG_T

Cuts at:   5697    5697 
   Size:       5821
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4935    5119    1305 
   Size:       3630     184    2007

  Fragments arranged by size: 

                3630    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2297      10 
   Size:         54    1425     343     465    3534

  Fragments arranged by size: 

                3534    1425     465     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2240    2426    2558    2564    2693    2786    3779 
   Size:        648    1074     186     132       6     129      93     993

Cuts at:   3779    4138    4297     518 
   Size:        359     159    2042

  Fragments arranged by size: 

                2042    1074     993     648     359     186     159     132
                 129      93       6
 
MspA1I CmG'CkG

Cuts at:   1606    2622    2887    3237    3914    5062    5307    1606 
   Size:       1016     265     350     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1016     677     350     265     245
 
NaeI GCC'GGC

Cuts at:   2026    2026 
   Size:       5821
 
NarI GG'CG_CC

Cuts at:   2028    2097    2808    2828    2028 
   Size:         69     711      20    5021

  Fragments arranged by size: 

                5021     711      69      20
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4330

  Fragments arranged by size: 

                4330     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5821
 
NgoMIV G'CCGG_C

Cuts at:   2024    2024 
   Size:       5821
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5821
 
NotI GC'GGCC_GC

Cuts at:   3016    3268    3016 
   Size:        252    5569
 
NspI r_CATG'y

Cuts at:   1132    1229    2602    3179    4724    1132 
   Size:         97    1373     577    1545    2229

  Fragments arranged by size: 

                2229    1545    1373     577      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5821
 
PciI A'CATG_T

Cuts at:   4720    4720 
   Size:       5821
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5821
 
PmeI GTTT'AAAC

Cuts at:   3125    3125 
   Size:       5821
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3890    3922    5403    5435    3890 
   Size:         32    1481      32    4276

  Fragments arranged by size: 

                4276    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2524    2524 
   Size:       5821
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5821
 
PsiI TTA'TAA

Cuts at:   3391    3391 
   Size:       5821
 
PstI C_TGCA'G

Cuts at:    830    2040    3173     830 
   Size:       1210    1133    3478

  Fragments arranged by size: 

                3478    1210    1133
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5821
 
PvuII CAG'CTG

Cuts at:   2622    2622 
   Size:       5821
 
SalI G'TCGA_C

Cuts at:   1936    3163    1936 
   Size:       1227    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2054    2054 
   Size:       5821
 
SbfI CC_TGCA'GG

Cuts at:   3173    3173 
   Size:       5821
 
ScaI AGT'ACT

Cuts at:   4069    5781    4069 
   Size:       1712    4109
 
SfcI C'TryA_G

Cuts at:    826    1045    2036    3169    4304    4985    5176     826 
   Size:        219     991    1133    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1133     991     681     219     191
 
SfoI GGC'GCC

Cuts at:   2029    2098    2809    2829    2029 
   Size:         69     711      20    5021

  Fragments arranged by size: 

                5021     711      69      20
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5821
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    2024    1700 
   Size:        324    5497
 
SmaI CCC'GGG

Cuts at:   1872    2579    3148    1872 
   Size:        707     569    4545

  Fragments arranged by size: 

                4545     707     569
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3925    4826    5088     820 
   Size:        197     926    1982     901     262    1553

  Fragments arranged by size: 

                1982    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3612     493 
   Size:       3119    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5821
 
SphI G_CATG'C

Cuts at:   1132    2602    3179    1132 
   Size:       1470     577    3774

  Fragments arranged by size: 

                3774    1470     577
 
SspI AAT'ATT

Cuts at:      5      52    3745       5 
   Size:         47    3693    2081

  Fragments arranged by size: 

                3693    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5821
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2493    2751    3260     513 
   Size:        552     939     489     258     509    3074

  Fragments arranged by size: 

                3074     939     552     509     489     258
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3848    3865    4018    4203    4648     325 
   Size:       1255    2268      17     153     185     445    1498

  Fragments arranged by size: 

                2268    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4067    5779      96 
   Size:        274      80      33      51    1443    2090    1712     138

  Fragments arranged by size: 

                2090    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5781    5813    5781 
   Size:         32    5789
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5821
 
XbaI T'CTAG_A

Cuts at:   3157    3157 
   Size:       5821
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2332    2557    2332 
   Size:        225    5596
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5821
 
XmaI C'CCGG_G

Cuts at:   1870    2577    3146    1870 
   Size:        707     569    4545

  Fragments arranged by size: 

                4545     707     569
 
XmnI GAAnn'nnTTC

Cuts at:   3950    3950 
   Size:       5821
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3629     276 
   Size:         53      83     186     936    2095    2468

  Fragments arranged by size: 

                2468    2095     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI
     PvuI    PvuII     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII    BbvCI    BfrBI     BplI
    BsiWI    BstXI  BstZ17I  EcoICRI    EcoNI    EcoRV     FseI    FspAI
     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI     PsrI
    RsrII     SacI    SacII    SanDI    SexAI     SfiI     SrfI     SwaI
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