Restriction map for
pFN21AB7195
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstF5I BstPI BstUI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DdeI DraI DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FokI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MfeI MluI
MroI MseI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflIII 202 ACATGT A^C[GA][CT]GT
AlwI 159 GGATC GGATC(4/5)
AlwNI 357 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 352 GGGCCC GGGCC^C
AspHI 251 GTGCTC G[AT]GC[AT]^C
AvaII 174 GGACC G^G[AT]CC
BalI 287 TGGCCA TGG^CCA
BanII 352 GGGCCC G[GA]GC[CT]^C
BbsI 3 GAAGAC GAAGAC(2/6)
BbvI 50 GCTGC GCAGC(8/12)
BcnI 244 CCCGG CC^[GC]GG
BpmI 10 CTCCAG CTGGAG(16/14)
BpuAI 3 GAAGAC GAAGAC(2/6)
BsiHKAI 251 GTGCTC G[AT]GC[AT]^C
BspLU11I 202 ACATGT A^CATGT
BsrDI 81 CATTGC GCAATG(2/0)
BstXI 155 CCAGGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 159 GGATCT [GA]^GATC[CT]
Cac8I 139 GCTGGC GC[ACGT]^[ACGT]GC
DpnII 160 GATC ^GATC
EaeI 287 TGGCCA [CT]^GGCC[GA]
Fnu4HI 50 GCTGC GC^[ACGT]GC
HapII 245 CCGG C^CGG
HinfI 265 GATTC G^A[ACGT]TC
HpaII 245 CCGG C^CGG
ItaI 50 GCTGC GC^[ACGT]GC
MboI 160 GATC ^GATC
MflI 159 GGATCT [GA]^GATC[CT]
MluNI 287 TGGCCA TGG^CCA
MnlI 344 GAGG CCTC(7/6)
MscI 287 TGGCCA TGG^CCA
MspI 245 CCGG C^CGG
NciI 244 CCCGG CC^[GC]GG
NdeII 160 GATC ^GATC
NspI 202 ACATGT [GA]CATG^[CT]
PpuMI 173 AGGACCC [GA]G^G[AT]CC[CT]
Sau3AI 160 GATC ^GATC
SfaNI 119 GCATC GCATC(5/9)
StuI 345 AGGCCT AGG^CCT
TfiI 265 GATTC G^A[AT]TC
TseI 50 GCTGC G^C[AT]GC
XcmI 294 CCAGTGTCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 159 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccI 223 GTATAC GT^[AC][GT]AC
273 GTATAC
AfaI 206 GTAC GT^AC
335 GTAC
BsaJI 155 CCAGGG C^C[ACGT][ACGT]GG
348 CCTGGG
Bst1107I 223 GTATAC GTA^TAC
273 GTATAC
BstZ17I 223 GTATAC GTA^TAC
273 GTATAC
DraII 173 AGGACCC [GA]G^G[ACGT]CC[CT]
351 GGGGCCC
DraIII 129 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
291 CACCCAGTG
EcoO109I 173 AGGACCC [GA]G^G[ACGT]CC[CT]
351 GGGGCCC
MaeIII 299 GTCAC ^GT[ACGT]AC
314 GTGAC
MwoI 50 GCTGCAATAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
253 GCTCACCATGC
NlaIII 203 CATG CATG^
259 CATG
RsaI 206 GTAC GT^AC
335 GTAC
Tsp45I 299 GTCAC ^GT[GC]AC
314 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BmyI Bsp1286I BsrI BssKI
BstNI Cfr13I EcoRII HaeIII HphI
MboII MvaI NlaIV Sau96I ScrFI
TspRI