(Circular) (Six-base) MAPSORT of: pFN21AB7192.seq Check: 8442 from: 1 to: 5656 pFN21AB7192 5656 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2375 2999 1937 Size: 438 624 4594 Fragments arranged by size: 4594 624 438 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2118 3719 5103 5371 1632 Size: 486 1601 1384 268 1917 Fragments arranged by size: 1917 1601 1384 486 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 4555 5532 1203 Size: 3352 977 1327 Fragments arranged by size: 3352 1327 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5656 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5656 AlwNI CAG_nnn'CTG Cuts at: 3399 4971 3399 Size: 1572 4084 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2065 2965 3191 3343 5452 1493 Size: 572 900 226 152 2109 1697 Fragments arranged by size: 2109 1697 900 572 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2981 1870 Size: 73 1038 4545 Fragments arranged by size: 4545 1038 73 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2537 2672 2804 2977 4432 Size: 325 217 627 750 135 132 173 1455 Cuts at: 4432 618 Size: 1842 Fragments arranged by size: 1842 1455 750 627 325 217 173 135 132 BanII G_rGCy'C Cuts at: 1391 2975 1391 Size: 1584 4072 BbeI G_GCGC'C Cuts at: 2808 2808 Size: 5656 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 2304 2646 2687 928 Size: 971 116 289 342 41 3897 Fragments arranged by size: 3897 971 342 289 116 41 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 1548 Size: 5656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4267 136 Size: 107 122 71 631 605 2595 1525 Fragments arranged by size: 2595 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5651 5651 Size: 5656 BlpI GC'TnA_GC Cuts at: 2027 3073 2027 Size: 1046 4610 Bme1580I G_kGCm'C Cuts at: 1790 2675 3717 4873 1790 Size: 885 1042 1156 2573 Fragments arranged by size: 2573 1156 1042 885 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2348 3387 4345 Size: 416 53 83 399 24 931 1039 958 Cuts at: 4345 442 Size: 1753 Fragments arranged by size: 1753 1039 958 931 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2646 4316 1239 Size: 1407 1670 2579 Fragments arranged by size: 2579 1670 1407 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3781 4646 4944 3781 Size: 865 298 4493 Fragments arranged by size: 4493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2645 4319 882 Size: 617 1146 1674 2219 Fragments arranged by size: 2219 1674 1146 617 BsaAI yAC'GTr Cuts at: 493 1204 3447 493 Size: 711 2243 2702 Fragments arranged by size: 2702 2243 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2355 2805 3463 3845 Size: 53 83 186 936 822 450 658 382 Cuts at: 3845 4480 275 Size: 635 1451 Fragments arranged by size: 1451 936 822 658 635 450 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2654 4089 4487 4761 4908 5319 2654 Size: 1435 398 274 147 411 2991 Fragments arranged by size: 2991 1435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2677 2707 1178 Size: 30 165 30 1274 30 4127 Fragments arranged by size: 4127 1274 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2249 2252 2264 2480 2601 1186 Size: 1063 3 12 216 121 4241 Fragments arranged by size: 4241 1063 216 121 12 3 BseYI C'CCAG_C Cuts at: 2411 4859 5355 2411 Size: 2448 496 2712 Fragments arranged by size: 2712 2448 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5083 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3106 3867 4895 1540 Size: 275 186 1105 761 1028 2301 Fragments arranged by size: 2301 1105 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3717 3802 4873 3717 Size: 85 1071 4500 Fragments arranged by size: 4500 1071 85 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2675 2975 3717 3802 4873 1391 Size: 399 885 300 742 85 1071 2174 Fragments arranged by size: 2174 1071 885 742 399 300 85 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3011 844 Size: 2167 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3543 1394 Size: 429 1720 3507 Fragments arranged by size: 3507 1720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4151 4325 65 Size: 1183 2903 174 1396 Fragments arranged by size: 2903 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4300 4487 1700 Size: 110 2490 187 2869 Fragments arranged by size: 2869 2490 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 2859 3716 4728 2859 Size: 857 1012 3787 Fragments arranged by size: 3787 1012 857 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5656 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5656 BstYI r'GATC_y Cuts at: 1972 2986 3353 3738 3755 5207 5293 5305 5651 Size: 1014 367 385 17 1452 86 12 346 Cuts at: 5651 1972 Size: 1977 Fragments arranged by size: 1977 1452 1014 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2376 2376 Size: 5656 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4917 Fragments arranged by size: 4917 683 56 BtsI GCAGTG_nn' Cuts at: 3172 3966 3986 3172 Size: 794 20 4842 Fragments arranged by size: 4842 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2755 2790 529 Size: 35 2191 35 3395 Fragments arranged by size: 3395 2191 35 35 DraI TTT'AAA Cuts at: 2169 2905 2960 2169 Size: 736 55 4865 Fragments arranged by size: 4865 736 55 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4663 809 Size: 507 3347 1802 Fragments arranged by size: 3347 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3103 3992 5621 Size: 54 1425 50 275 18 1273 889 1629 Cuts at: 5621 8 Size: 43 Fragments arranged by size: 1629 1425 1273 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3103 1537 Size: 275 1291 4090 Fragments arranged by size: 4090 1291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2071 2226 2241 2457 2466 3586 1353 Size: 718 155 15 216 9 1120 3423 Fragments arranged by size: 3423 1120 718 216 155 15 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4243 4617 4763 387 Size: 1064 138 2654 374 146 1280 Fragments arranged by size: 2654 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2118 2646 3719 4316 5103 5371 1239 Size: 393 486 528 1073 597 787 268 1524 Fragments arranged by size: 1524 1073 787 597 528 486 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2367 2367 Size: 5656 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 4162 4162 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 2808 4803 1678 Size: 1130 1995 2531 Fragments arranged by size: 2531 1995 1130 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2285 2317 2817 2849 4297 Size: 32 135 32 713 32 500 32 1448 Cuts at: 4297 4329 4371 4403 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1448 713 500 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KasI G'GCGC_C Cuts at: 2804 2804 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3834 Fragments arranged by size: 3834 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3614 3973 4132 518 Size: 648 2448 359 159 2042 Fragments arranged by size: 2448 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2583 3072 3749 4897 5142 1606 Size: 977 489 677 1148 245 2120 Fragments arranged by size: 2120 1148 977 677 489 245 NarI GG'CG_CC Cuts at: 2805 2805 Size: 5656 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5104 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 2150 3014 4559 1132 Size: 97 921 864 1545 2229 Fragments arranged by size: 2229 1545 921 864 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5656 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 2702 2756 1108 Size: 1594 54 4008 Fragments arranged by size: 4008 1594 54 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3725 3757 5238 5270 3725 Size: 32 1481 32 4111 Fragments arranged by size: 4111 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PstI C_TGCA'G Cuts at: 830 3008 830 Size: 2178 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SexAI A'CCwGG_T Cuts at: 2952 2952 Size: 5656 SfcI C'TryA_G Cuts at: 826 1045 2734 3004 4139 4820 5011 826 Size: 219 1689 270 1135 681 191 1471 Fragments arranged by size: 1689 1471 1135 681 270 219 191 SfoI GGC'GCC Cuts at: 2806 2806 Size: 5656 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3760 4661 4923 820 Size: 197 926 1817 901 262 1553 Fragments arranged by size: 1817 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 2150 3014 1132 Size: 1018 864 3774 Fragments arranged by size: 3774 1018 864 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5656 StyI C'CwwG_G Cuts at: 513 1065 3095 513 Size: 552 2030 3074 Fragments arranged by size: 3074 2030 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3683 3700 3853 4038 4483 325 Size: 1255 2103 17 153 185 445 1498 Fragments arranged by size: 2103 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 2892 3902 Size: 274 80 33 51 1443 141 774 1010 Cuts at: 3902 5614 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1010 774 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5616 5648 5616 Size: 32 5624 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 2743 3785 2743 Size: 1042 4614 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI