(Circular) (Six-base) MAPSORT of: pFN21AB7187.seq Check: 7486 from: 1 to: 6322 pFN21AB7187 6322 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3186 3186 Size: 6322 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4132 278 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3665 1937 Size: 1728 4594 Acc65I G'GTAC_C Cuts at: 3643 3643 Size: 6322 AclI AA'CG_TT Cuts at: 1563 4449 4822 1563 Size: 2886 373 3063 Fragments arranged by size: 3063 2886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3590 4385 5769 6037 1632 Size: 1958 795 1384 268 1917 Fragments arranged by size: 1958 1917 1384 795 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6125 AflIII A'CryG_T Cuts at: 1203 5221 6198 1203 Size: 4018 977 1327 Fragments arranged by size: 4018 1327 977 AgeI A'CCGG_T Cuts at: 5153 5153 Size: 6322 AhdI GACnn_n'nnGTC Cuts at: 5051 5051 Size: 6322 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6322 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6322 AlwNI CAG_nnn'CTG Cuts at: 2207 2498 4065 5637 2207 Size: 291 1567 1572 2892 Fragments arranged by size: 2892 1572 1567 291 ApaI G_GGCC'C Cuts at: 2030 2937 2030 Size: 907 5415 ApaLI G'TGCA_C Cuts at: 4379 5535 4379 Size: 1156 5166 ApoI r'AATT_y Cuts at: 1493 3389 3631 3857 4009 6118 1493 Size: 1896 242 226 152 2109 1697 Fragments arranged by size: 2109 1896 1697 242 226 152 AseI AT'TA_AT Cuts at: 160 1032 4876 160 Size: 872 3844 1606 Fragments arranged by size: 3844 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 AvaI C'yCGr_G Cuts at: 1870 1943 2029 2405 2936 3647 1870 Size: 73 86 376 531 711 4545 Fragments arranged by size: 4545 711 531 376 86 73 BamHI G'GATC_C Cuts at: 3652 4019 3652 Size: 367 5955 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2233 2294 2594 3643 5098 Size: 325 217 627 446 61 300 1049 1455 Cuts at: 5098 618 Size: 1842 Fragments arranged by size: 1842 1455 1049 627 446 325 300 217 61 BanII G_rGCy'C Cuts at: 1391 2030 2492 2519 2607 2937 3287 3411 3452 Size: 639 462 27 88 330 350 124 41 Cuts at: 3452 3641 1391 Size: 189 4072 Fragments arranged by size: 4072 639 462 350 330 189 124 88 41 27 BbeI G_GCGC'C Cuts at: 2237 2237 Size: 6322 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3596 928 Size: 971 1697 3654 Fragments arranged by size: 3654 1697 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2995 3029 4513 4547 1903 Size: 34 1058 34 1484 34 3678 Fragments arranged by size: 3678 1484 1058 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4214 5424 4214 Size: 1210 5112 BclI T'GATC_A Cuts at: 1548 1548 Size: 6322 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2090 2099 3087 Size: 107 122 71 631 605 418 9 988 Cuts at: 3087 4933 136 Size: 1846 1525 Fragments arranged by size: 1846 1525 988 631 605 418 122 107 71 9 BglII A'GATC_T Cuts at: 2674 6317 2674 Size: 3643 2679 BlpI GC'TnA_GC Cuts at: 2486 3739 2486 Size: 1253 5069 Bme1580I G_kGCm'C Cuts at: 1790 2030 2597 2913 2937 4383 5539 1790 Size: 240 567 316 24 1446 1156 2573 Fragments arranged by size: 2573 1446 1156 567 316 240 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6322 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2925 4053 5011 Size: 416 53 83 399 24 1508 1128 958 Cuts at: 5011 442 Size: 1753 Fragments arranged by size: 1753 1508 1128 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2369 2621 2767 3043 4982 1239 Size: 1130 252 146 276 1939 2579 Fragments arranged by size: 2579 1939 1130 276 252 146 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2547 3338 3485 3581 4447 5312 5610 2547 Size: 791 147 96 866 865 298 3259 Fragments arranged by size: 3259 866 865 791 298 147 96 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2729 2874 4985 882 Size: 617 1230 145 2111 2219 Fragments arranged by size: 2219 2111 1230 617 145 BsaAI yAC'GTr Cuts at: 493 1204 4113 493 Size: 711 2909 2702 Fragments arranged by size: 2909 2702 711 BsaBI GATnn'nnATC Cuts at: 6316 6316 Size: 6322 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2222 2234 4129 4511 Size: 53 83 186 936 689 12 1895 382 Cuts at: 4511 5146 275 Size: 635 1451 Fragments arranged by size: 1895 1451 936 689 635 382 186 83 53 12 BsaWI w'CCGG_w Cuts at: 3393 4755 5153 5427 5574 5985 3393 Size: 1362 398 274 147 411 3730 Fragments arranged by size: 3730 1362 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3041 3071 1178 Size: 30 165 30 1638 30 4429 Fragments arranged by size: 4429 1638 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2346 2852 2855 2864 3533 3536 1186 Size: 1160 506 3 9 669 3 3972 Fragments arranged by size: 3972 1160 669 506 9 3 3 BseYI C'CCAG_C Cuts at: 2207 2491 5525 6021 2207 Size: 284 3034 496 2508 Fragments arranged by size: 3034 2508 496 284 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5749 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3772 4533 5561 1540 Size: 275 186 1771 761 1028 2301 Fragments arranged by size: 2301 1771 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3411 3478 4383 4468 5539 3411 Size: 67 905 85 1071 4194 Fragments arranged by size: 4194 1071 905 85 67 BsmI GAATG_Cn' Cuts at: 3833 3926 3833 Size: 93 6229 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2492 2519 2597 2607 2913 2937 Size: 399 240 462 27 78 10 306 24 Cuts at: 2937 3287 3411 3452 3478 3641 4383 4468 5539 Size: 350 124 41 26 163 742 85 1071 Cuts at: 5539 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 462 399 350 306 240 163 124 85 78 41 27 26 24 10 BspEI T'CCGG_A Cuts at: 3393 5985 3393 Size: 2592 3730 BspHI T'CATG_A Cuts at: 3315 4211 5941 3315 Size: 896 1730 3696 Fragments arranged by size: 3696 1730 896 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3186 3357 3677 844 Size: 2342 171 320 3489 Fragments arranged by size: 3489 2342 320 171 BsrBI CCG'CTC Cuts at: 1394 1823 4209 1394 Size: 429 2386 3507 Fragments arranged by size: 3507 2386 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3438 4817 4991 65 Size: 1183 2190 1379 174 1396 Fragments arranged by size: 2190 1396 1379 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2098 2192 2291 2611 3114 4966 5153 Size: 110 288 94 99 320 503 1852 187 Cuts at: 5153 1700 Size: 2869 Fragments arranged by size: 2869 1852 503 320 288 187 110 99 94 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6322 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6322 BssSI C'ACGA_G Cuts at: 2396 4382 5394 2396 Size: 1986 1012 3324 Fragments arranged by size: 3324 1986 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3078 794 Size: 2284 4038 BstBI TT'CG_AA Cuts at: 2643 5126 2643 Size: 2483 3839 BstEII G'GTnAC_C Cuts at: 1184 2287 2694 3575 1184 Size: 1103 407 881 3931 Fragments arranged by size: 3931 1103 881 407 BstYI r'GATC_y Cuts at: 1972 2674 3652 4019 4404 4421 5873 5959 5971 Size: 702 978 367 385 17 1452 86 12 Cuts at: 5971 6317 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 978 702 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2436 3020 2436 Size: 584 5738 BtgI C'CryG_G Cuts at: 513 1065 2004 3279 513 Size: 552 939 1275 3556 Fragments arranged by size: 3556 1275 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2373 2998 487 Size: 683 56 1147 625 3811 Fragments arranged by size: 3811 1147 683 625 56 BtsI GCAGTG_nn' Cuts at: 2048 3091 3838 4632 4652 2048 Size: 1043 747 794 20 3718 Fragments arranged by size: 3718 1043 794 747 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6322 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6287 DraI TTT'AAA Cuts at: 3626 3626 Size: 6322 DraIII CAC_nnn'GTG Cuts at: 3078 3078 Size: 6322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5329 809 Size: 507 4013 1802 Fragments arranged by size: 4013 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 3157 3276 Size: 54 1425 50 275 18 420 907 119 Cuts at: 3276 3769 4658 6287 8 Size: 493 889 1629 43 Fragments arranged by size: 1629 1425 907 889 493 420 275 119 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3769 1537 Size: 275 1957 4090 Fragments arranged by size: 4090 1957 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 2256 2829 2841 3137 3317 4252 1353 Size: 774 129 573 12 296 180 935 3423 Fragments arranged by size: 3423 935 774 573 296 180 129 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4909 5283 5429 387 Size: 1064 138 3320 374 146 1280 Fragments arranged by size: 3320 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3409 3409 Size: 6322 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2369 2621 2767 3043 3590 4385 4982 Size: 393 737 252 146 276 547 795 597 Cuts at: 4982 5769 6037 1239 Size: 787 268 1524 Fragments arranged by size: 1524 795 787 737 597 547 393 276 268 252 146 EcoNI CCTnn'n_nnAGG Cuts at: 3193 3515 3193 Size: 322 6000 EcoO109I rG'GnC_Cy Cuts at: 2026 2368 2401 2933 3024 3186 2026 Size: 342 33 532 91 162 5162 Fragments arranged by size: 5162 532 342 162 91 33 EcoRI G'AATT_C Cuts at: 3631 4009 3631 Size: 378 5944 EcoRV GAT'ATC Cuts at: 2623 2623 Size: 6322 FspI TGC'GCA Cuts at: 4828 4828 Size: 6322 HaeII r_GCGC'y Cuts at: 1678 2237 5469 1678 Size: 559 3232 2531 Fragments arranged by size: 3232 2531 559 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2207 2239 3041 3073 3410 Size: 32 135 32 635 32 802 32 337 Cuts at: 3410 3442 3509 3541 4963 4995 5037 5069 1373 Size: 32 67 32 1422 32 42 32 2626 Fragments arranged by size: 2626 1422 802 635 337 135 67 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3666 3912 669 Size: 988 281 1728 246 3079 Fragments arranged by size: 3079 1728 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6322 HpaI GTT'AAC Cuts at: 3912 3912 Size: 6322 KasI G'GCGC_C Cuts at: 2233 2233 Size: 6322 KpnI G_GTAC'C Cuts at: 3647 3647 Size: 6322 MfeI C'AATT_G Cuts at: 3921 3921 Size: 6322 MluI A'CGCG_T Cuts at: 6198 6198 Size: 6322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5436 5620 1305 Size: 4131 184 2007 Fragments arranged by size: 4131 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4500 Fragments arranged by size: 4500 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3459 4280 4639 4798 518 Size: 648 2293 821 359 159 2042 Fragments arranged by size: 2293 2042 821 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2204 2355 2454 3738 4415 5563 5808 1606 Size: 598 151 99 1284 677 1148 245 2120 Fragments arranged by size: 2120 1284 1148 677 598 245 151 99 NaeI GCC'GGC Cuts at: 2100 2194 2100 Size: 94 6228 NarI GG'CG_CC Cuts at: 2234 2234 Size: 6322 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4831 Fragments arranged by size: 4831 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6322 NgoMIV G'CCGG_C Cuts at: 2098 2192 2098 Size: 94 6228 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6322 NotI GC'GGCC_GC Cuts at: 3769 3769 Size: 6322 NspI r_CATG'y Cuts at: 1132 1229 2969 3680 5225 1132 Size: 97 1740 711 1545 2229 Fragments arranged by size: 2229 1740 1545 711 97 PasI CC'CwG_GG Cuts at: 1348 2690 1348 Size: 1342 4980 PciI A'CATG_T Cuts at: 5221 5221 Size: 6322 PflMI CCAn_nnn'nTGG Cuts at: 1108 2207 2961 1108 Size: 1099 754 4469 Fragments arranged by size: 4469 1099 754 PfoI T'CCnGG_A Cuts at: 2744 3489 2744 Size: 745 5577 PmeI GTTT'AAAC Cuts at: 3626 3626 Size: 6322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4391 4423 5904 5936 4391 Size: 32 1481 32 4777 Fragments arranged by size: 4777 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2401 2401 Size: 6322 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6322 PsiI TTA'TAA Cuts at: 3892 3892 Size: 6322 PspOMI G'GGCC_C Cuts at: 2026 2933 2026 Size: 907 5415 PstI C_TGCA'G Cuts at: 830 2274 3197 3674 830 Size: 1444 923 477 3478 Fragments arranged by size: 3478 1444 923 477 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6322 SacI G_AGCT'C Cuts at: 3411 3411 Size: 6322 SalI G'TCGA_C Cuts at: 1936 3664 1936 Size: 1728 4594 SapI GCTCTTCn'nnn_ Cuts at: 2127 3317 2127 Size: 1190 5132 SbfI CC_TGCA'GG Cuts at: 3674 3674 Size: 6322 ScaI AGT'ACT Cuts at: 4570 6282 4570 Size: 1712 4610 SexAI A'CCwGG_T Cuts at: 3168 3168 Size: 6322 SfcI C'TryA_G Cuts at: 826 1045 2270 2442 3193 3670 4805 5486 5677 Size: 219 1225 172 751 477 1135 681 191 Cuts at: 5677 826 Size: 1471 Fragments arranged by size: 1471 1225 1135 751 681 477 219 191 172 SfiI GGCCn_nnn'nGGCC Cuts at: 2090 2090 Size: 6322 SfoI GGC'GCC Cuts at: 2235 2235 Size: 6322 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6322 SmaI CCC'GGG Cuts at: 1872 2031 2938 3649 1872 Size: 159 907 711 4545 Fragments arranged by size: 4545 907 711 159 SmlI C'TyrA_G Cuts at: 820 1017 1943 2562 3353 3500 3560 4426 5327 Size: 197 926 619 791 147 60 866 901 Cuts at: 5327 5589 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 866 791 619 262 197 147 60 SnaBI TAC'GTA Cuts at: 493 4113 493 Size: 3620 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6322 SphI G_CATG'C Cuts at: 1132 2969 3680 1132 Size: 1837 711 3774 Fragments arranged by size: 3774 1837 711 SrfI GCCC'GGGC Cuts at: 2031 2031 Size: 6322 SspI AAT'ATT Cuts at: 5 52 4246 5 Size: 47 4194 2081 Fragments arranged by size: 4194 2081 47 StuI AGG'CCT Cuts at: 1517 2325 1517 Size: 808 5514 StyI C'CwwG_G Cuts at: 513 1065 2004 2508 2520 3761 513 Size: 552 939 504 12 1241 3074 Fragments arranged by size: 3074 1241 939 552 504 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2986 3471 4349 4366 4519 4704 5149 Size: 1255 1406 485 878 17 153 185 445 Cuts at: 5149 325 Size: 1498 Fragments arranged by size: 1498 1406 1255 878 485 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4568 6280 96 Size: 274 80 33 51 1443 2591 1712 138 Fragments arranged by size: 2591 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6282 6314 6282 Size: 32 6290 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6322 XbaI T'CTAG_A Cuts at: 3658 3658 Size: 6322 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6322 XmaI C'CCGG_G Cuts at: 1870 2029 2936 3647 1870 Size: 159 907 711 4545 Fragments arranged by size: 4545 907 711 159 XmnI GAAnn'nnTTC Cuts at: 4451 4451 Size: 6322 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4130 276 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SwaI XcmI