Restriction map for
pFN21AB7187
(Linear) (Six-base) MAPSORT of: KIBB7187.seq Check: 3823 from: 1 to: 1617
KIBB7187.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1181 1617
Size: 1181 436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1585 1617
Size: 1585 32
AlwNI CAG_nnn'CTG
Cuts at: 0 202 493 1617
Size: 202 291 1124
Fragments arranged by size:
1124 291 202
ApaI G_GGCC'C
Cuts at: 0 25 932 1617
Size: 25 907 685
Fragments arranged by size:
907 685 25
ApoI r'AATT_y
Cuts at: 0 1384 1617
Size: 1384 233
AvaI C'yCGr_G
Cuts at: 0 24 400 931 1617
Size: 24 376 531 686
Fragments arranged by size:
686 531 376 24
BanI G'GyrC_C
Cuts at: 0 228 289 589 1617
Size: 228 61 300 1028
Fragments arranged by size:
1028 300 228 61
BanII G_rGCy'C
Cuts at: 0 25 487 514 602 932 1282 1406 1447
Size: 25 462 27 88 330 350 124 41
Cuts at: 1447 1617
Size: 170
Fragments arranged by size:
462 350 330 170 124 88 41 27
25
BbeI G_GCGC'C
Cuts at: 0 232 1617
Size: 232 1385
BbsI GAAGACnn'nnnn_
Cuts at: 0 1591 1617
Size: 1591 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 990 1024 1617
Size: 990 34 593
Fragments arranged by size:
990 593 34
BglI GCCn_nnn'nGGC
Cuts at: 0 85 94 1082 1617
Size: 85 9 988 535
Fragments arranged by size:
988 535 85 9
BglII A'GATC_T
Cuts at: 0 669 1617
Size: 669 948
BlpI GC'TnA_GC
Cuts at: 0 481 1617
Size: 481 1136
Bme1580I G_kGCm'C
Cuts at: 0 25 592 908 932 1617
Size: 25 567 316 24 685
Fragments arranged by size:
685 567 316 25 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 920 1617
Size: 920 697
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 616 762 1038 1617
Size: 364 252 146 276 579
Fragments arranged by size:
579 364 276 252 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 1333 1480 1576 1617
Size: 542 791 147 96 41
Fragments arranged by size:
791 542 147 96 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 724 869 1617
Size: 724 145 748
Fragments arranged by size:
748 724 145
BsaHI Gr'CG_yC
Cuts at: 0 217 229 1617
Size: 217 12 1388
Fragments arranged by size:
1388 217 12
BsaWI w'CCGG_w
Cuts at: 0 1388 1617
Size: 1388 229
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1036 1066 1617
Size: 1036 30 551
Fragments arranged by size:
1036 551 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 341 847 850 859 1528 1531 1617
Size: 341 506 3 9 669 3 86
Fragments arranged by size:
669 506 341 86 9 3 3
BseYI C'CCAG_C
Cuts at: 0 202 486 1617
Size: 202 284 1131
Fragments arranged by size:
1131 284 202
BsiHKAI G_wGCw'C
Cuts at: 0 1406 1473 1617
Size: 1406 67 144
Fragments arranged by size:
1406 144 67
Bsp1286I G_dGCh'C
Cuts at: 0 25 487 514 592 602 908 932 1282
Size: 25 462 27 78 10 306 24 350
Cuts at: 1282 1406 1447 1473 1617
Size: 124 41 26 144
Fragments arranged by size:
462 350 306 144 124 78 41 27
26 25 24 10
BspEI T'CCGG_A
Cuts at: 0 1388 1617
Size: 1388 229
BspHI T'CATG_A
Cuts at: 0 1310 1617
Size: 1310 307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1181 1352 1617
Size: 1181 171 265
Fragments arranged by size:
1181 265 171
BsrDI GCAATG_nn'
Cuts at: 0 1433 1617
Size: 1433 184
BsrFI r'CCGG_y
Cuts at: 0 93 187 286 606 1109 1617
Size: 93 94 99 320 503 508
Fragments arranged by size:
508 503 320 99 94 93
BssSI C'ACGA_G
Cuts at: 0 391 1617
Size: 391 1226
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1073 1617
Size: 1073 544
BstBI TT'CG_AA
Cuts at: 0 638 1617
Size: 638 979
BstEII G'GTnAC_C
Cuts at: 0 282 689 1570 1617
Size: 282 407 881 47
Fragments arranged by size:
881 407 282 47
BstYI r'GATC_y
Cuts at: 0 669 1617
Size: 669 948
Bsu36I CC'TnA_GG
Cuts at: 0 431 1015 1617
Size: 431 584 602
Fragments arranged by size:
602 584 431
BtgI C'CryG_G
Cuts at: 0 1274 1617
Size: 1274 343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 368 993 1617
Size: 368 625 624
Fragments arranged by size:
625 624 368
BtsI GCAGTG_nn'
Cuts at: 0 43 1086 1617
Size: 43 1043 531
Fragments arranged by size:
1043 531 43
DraIII CAC_nnn'GTG
Cuts at: 0 1073 1617
Size: 1073 544
EaeI y'GGCC_r
Cuts at: 0 245 1152 1271 1617
Size: 245 907 119 346
Fragments arranged by size:
907 346 245 119
EarI CTCTTCn'nnn_
Cuts at: 0 122 251 824 836 1132 1312 1617
Size: 122 129 573 12 296 180 305
Fragments arranged by size:
573 305 296 180 129 122 12
EcoICRI GAG'CTC
Cuts at: 0 1404 1617
Size: 1404 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 616 762 1038 1585 1617
Size: 364 252 146 276 547 32
Fragments arranged by size:
547 364 276 252 146 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1188 1510 1617
Size: 1188 322 107
Fragments arranged by size:
1188 322 107
EcoO109I rG'GnC_Cy
Cuts at: 0 21 363 396 928 1019 1181 1617
Size: 21 342 33 532 91 162 436
Fragments arranged by size:
532 436 342 162 91 33 21
EcoRV GAT'ATC
Cuts at: 0 618 1617
Size: 618 999
HaeII r_GCGC'y
Cuts at: 0 232 1617
Size: 232 1385
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 1036 1068 1405 1437 1504 1536
Size: 202 32 802 32 337 32 67 32
Cuts at: 1536 1617
Size: 81
Fragments arranged by size:
802 337 202 81 67 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 228 1617
Size: 228 1389
MslI CAynn'nnrTG
Cuts at: 0 1454 1617
Size: 1454 163
MspA1I CmG'CkG
Cuts at: 0 199 350 449 1617
Size: 199 151 99 1168
Fragments arranged by size:
1168 199 151 99
NaeI GCC'GGC
Cuts at: 0 95 189 1617
Size: 95 94 1428
Fragments arranged by size:
1428 95 94
NarI GG'CG_CC
Cuts at: 0 229 1617
Size: 229 1388
NgoMIV G'CCGG_C
Cuts at: 0 93 187 1617
Size: 93 94 1430
Fragments arranged by size:
1430 94 93
NspI r_CATG'y
Cuts at: 0 964 1617
Size: 964 653
PasI CC'CwG_GG
Cuts at: 0 685 1617
Size: 685 932
PflMI CCAn_nnn'nTGG
Cuts at: 0 202 956 1617
Size: 202 754 661
Fragments arranged by size:
754 661 202
PfoI T'CCnGG_A
Cuts at: 0 739 1484 1617
Size: 739 745 133
Fragments arranged by size:
745 739 133
PpuMI rG'GwC_Cy
Cuts at: 0 396 1617
Size: 396 1221
PspOMI G'GGCC_C
Cuts at: 0 21 928 1617
Size: 21 907 689
Fragments arranged by size:
907 689 21
PstI C_TGCA'G
Cuts at: 0 269 1192 1617
Size: 269 923 425
Fragments arranged by size:
923 425 269
SacI G_AGCT'C
Cuts at: 0 1406 1617
Size: 1406 211
SapI GCTCTTCn'nnn_
Cuts at: 0 122 1312 1617
Size: 122 1190 305
Fragments arranged by size:
1190 305 122
SexAI A'CCwGG_T
Cuts at: 0 1163 1617
Size: 1163 454
SfcI C'TryA_G
Cuts at: 0 265 437 1188 1617
Size: 265 172 751 429
Fragments arranged by size:
751 429 265 172
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 85 1617
Size: 85 1532
SfoI GGC'GCC
Cuts at: 0 230 1617
Size: 230 1387
SmaI CCC'GGG
Cuts at: 0 26 933 1617
Size: 26 907 684
Fragments arranged by size:
907 684 26
SmlI C'TyrA_G
Cuts at: 0 557 1348 1495 1555 1617
Size: 557 791 147 60 62
Fragments arranged by size:
791 557 147 62 60
SphI G_CATG'C
Cuts at: 0 964 1617
Size: 964 653
SrfI GCCC'GGGC
Cuts at: 0 26 1617
Size: 26 1591
StuI AGG'CCT
Cuts at: 0 320 1617
Size: 320 1297
StyI C'CwwG_G
Cuts at: 0 503 515 1617
Size: 503 12 1102
Fragments arranged by size:
1102 503 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 981 1466 1617
Size: 981 485 151
Fragments arranged by size:
981 485 151
XmaI C'CCGG_G
Cuts at: 0 24 931 1617
Size: 24 907 686
Fragments arranged by size:
907 686 24
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApoI AvaI BanI BanII
BbeI BbsI BcgI BglI BglII BlpI Bme1580I BmrI
BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
HaeII Hin4I KasI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PflMI PfoI PpuMI PspOMI PstI SacI
SapI SexAI SfcI SfiI SfoI SmaI SmlI SphI
SrfI StuI StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BmgBI
BmtI BplI Bpu10I BsaAI BsaBI BsgI BsiEI BsiWI
BsmI BsmBI BsrBI BsrGI BssHII BstXI BstZ17I ClaI
CspCI DraI DrdI EagI EciI EcoRI FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI
ScaI SgfI SgrAI SnaBI SpeI SspI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI