Restriction map for pFN21AB7158
 (Circular) (Six-base) MAPSORT of: pFN21AB7158.seq  Check: 1228  from: 1  to: 5794



pFN21AB7158  5794 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2420    2420 
   Size:       5794
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2556    3604     278 
   Size:         53      83     186     936    1020    1048    2468

  Fragments arranged by size: 

                2468    1048    1020     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2096    3137    1937 
   Size:        159    1041    4594

  Fragments arranged by size: 

                4594    1041     159
 
Acc65I G'GTAC_C

Cuts at:   2430    3115    2430 
   Size:        685    5109
 
AclI AA'CG_TT

Cuts at:   1563    3921    4294    1563 
   Size:       2358     373    3063

  Fragments arranged by size: 

                3063    2358     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2741    2789    3857    5241    5509    1632 
   Size:       1109      48    1068    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1109    1068     268      48
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5597
 
AflIII A'CryG_T

Cuts at:   1203    2385    4693    5670    1203 
   Size:       1182    2308     977    1327

  Fragments arranged by size: 

                2308    1327    1182     977
 
AgeI A'CCGG_T

Cuts at:   2973    4625    2973 
   Size:       1652    4142
 
AhdI GACnn_n'nnGTC

Cuts at:   4523    4523 
   Size:       5794
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5794
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2154    1184 
   Size:        970    4824
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3046    3078    3046 
   Size:         32    5762
 
AlwNI CAG_nnn'CTG

Cuts at:   2783    3537    5109    2783 
   Size:        754    1572    3468

  Fragments arranged by size: 

                3468    1572     754
 
ApaLI G'TGCA_C

Cuts at:   3851    5007    3851 
   Size:       1156    4638
 
ApoI r'AATT_y

Cuts at:   1493    3103    3329    3481    5590    1493 
   Size:       1610     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1610     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2865    4348     160 
   Size:        872    1833    1483    1606

  Fragments arranged by size: 

                1833    1606    1483     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5794
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3119    1870 
   Size:         73    1176    4545

  Fragments arranged by size: 

                4545    1176      73
 
BamHI G'GATC_C

Cuts at:   3124    3491    3124 
   Size:        367    5427
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2430    3115    4570     618 
   Size:        325     217     627     643     685    1455    1842

  Fragments arranged by size: 

                1842    1455     685     643     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3113    1391 
   Size:       1722    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2048    2729     928 
   Size:        971     149     681    3993

  Fragments arranged by size: 

                3993     971     681     149
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3985    4019    1903 
   Size:         34    2048      34    3678

  Fragments arranged by size: 

                3678    2048      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3686    4896    3686 
   Size:       1210    4584
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5794
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4405     136 
   Size:        107     122      71     631     605    2733    1525

  Fragments arranged by size: 

                2733    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5789    5789 
   Size:       5794
 
BlpI GC'TnA_GC

Cuts at:   3211    3211 
   Size:       5794
 
Bme1580I G_kGCm'C

Cuts at:   1790    3855    5011    1790 
   Size:       2065    1156    2573

  Fragments arranged by size: 

                2573    2065    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5794
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3525    4483     442 
   Size:        416      53      83     399      24    2108     958    1753

  Fragments arranged by size: 

                2108    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5794
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4454    1239 
   Size:       3215    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3919    4784    5082    3919 
   Size:        865     298    4631

  Fragments arranged by size: 

                4631     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3074    4457     882 
   Size:        617    1575    1383    2219

  Fragments arranged by size: 

                2219    1575    1383     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3585     493 
   Size:        711    2381    2702

  Fragments arranged by size: 

                2702    2381     711
 
BsaBI GATnn'nnATC

Cuts at:   5788    5788 
   Size:       5794
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2553    3601    3983    4618 
   Size:         53      83     186     936    1020    1048     382     635

Cuts at:   4618     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1048    1020     936     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2973    4227    4625    4899    5046    5457    2973 
   Size:       1254     398     274     147     411    3310

  Fragments arranged by size: 

                3310    1254     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2973    3003    1178 
   Size:         30     165      30    1570      30    3969

  Fragments arranged by size: 

                3969    1570     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5794
 
BseYI C'CCAG_C

Cuts at:   2630    4997    5493    2630 
   Size:       2367     496    2931

  Fragments arranged by size: 

                2931    2367     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5221
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3244    4005    5033    1540 
   Size:        275     186    1243     761    1028    2301

  Fragments arranged by size: 

                2301    1243    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3855    3940    5011    3855 
   Size:         85    1071    4638

  Fragments arranged by size: 

                4638    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2587    3305    3398    2587 
   Size:        718      93    4983

  Fragments arranged by size: 

                4983     718      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2544    2544 
   Size:       5794
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3113    3855    3940    5011    1391 
   Size:        399    1323     742      85    1071    2174

  Fragments arranged by size: 

                2174    1323    1071     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5457    5457 
   Size:       5794
 
BspHI T'CATG_A

Cuts at:   3683    5413    3683 
   Size:       1730    4064
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2420    3149     844 
   Size:       1576     729    3489

  Fragments arranged by size: 

                3489    1576     729
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3681    1394 
   Size:        429    1858    3507

  Fragments arranged by size: 

                3507    1858     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2528    4289    4463      65 
   Size:       1183    1280    1761     174    1396

  Fragments arranged by size: 

                1761    1396    1280    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2973    4438    4625    1700 
   Size:        110    1163    1465     187    2869

  Fragments arranged by size: 

                2869    1465    1163     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5794
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5794
 
BssSI C'ACGA_G

Cuts at:   3854    4866    3854 
   Size:       1012    4782
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5794
 
BstBI TT'CG_AA

Cuts at:   4598    4598 
   Size:       5794
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5794
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2920    3018    2920 
   Size:         98    5696
 
BstYI r'GATC_y

Cuts at:   1972    3124    3491    3876    3893    5345    5431    5443    5789 
   Size:       1152     367     385      17    1452      86      12     346

Cuts at:   5789    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1152     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5242
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5055

  Fragments arranged by size: 

                5055     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3001    3310    4104    4124    3001 
   Size:        309     794      20    4671

  Fragments arranged by size: 

                4671     794     309      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5794
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5759
 
DraI TTT'AAA

Cuts at:   3098    3098 
   Size:       5794
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3088    4801     809 
   Size:        507    1772    1713    1802

  Fragments arranged by size: 

                1802    1772    1713     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3241    4130    5759 
   Size:         54    1425      50     275      18    1411     889    1629

Cuts at:   5759       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1411     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3241    1537 
   Size:        275    1429    4090

  Fragments arranged by size: 

                4090    1429     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2298    3724    1353 
   Size:        945    1426    3423

  Fragments arranged by size: 

                3423    1426     945
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4381    4755    4901     387 
   Size:       1064     138    2792     374     146    1280

  Fragments arranged by size: 

                2792    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2741    2789    3857    4454    5241    5509    1239 
   Size:        393    1109      48    1068     597     787     268    1524

  Fragments arranged by size: 

                1524    1109    1068     787     597     393     268      48
            
 
EcoO109I rG'GnC_Cy

Cuts at:   2225    2225 
   Size:       5794
 
EcoRI G'AATT_C

Cuts at:   3103    3481    3103 
   Size:        378    5416
 
FspI TGC'GCA

Cuts at:   4300    4300 
   Size:       5794
 
HaeII r_GCGC'y

Cuts at:   1678    4941    1678 
   Size:       3263    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2169    2201    3029    3061    4435 
   Size:         32     135      32     597      32     828      32    1374

Cuts at:   4435    4467    4509    4541    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1374     828     597     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3138    3384     669 
   Size:        988     281    1200     246    3079

  Fragments arranged by size: 

                3079    1200     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5794
 
HpaI GTT'AAC

Cuts at:   3384    3384 
   Size:       5794
 
KpnI G_GTAC'C

Cuts at:   2434    3119    2434 
   Size:        685    5109
 
MfeI C'AATT_G

Cuts at:   3393    3393 
   Size:       5794
 
MluI A'CGCG_T

Cuts at:   5670    5670 
   Size:       5794
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2947    3004    4908    5092    1305 
   Size:       1642      57    1904     184    2007

  Fragments arranged by size: 

                2007    1904    1642     184      57
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3972

  Fragments arranged by size: 

                3972    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2136    2414    3752    4111    4270     518 
   Size:        648     970     278    1338     359     159    2042

  Fragments arranged by size: 

                2042    1338     970     648     359     278     159
 
MspA1I CmG'CkG

Cuts at:   1606    2052    2630    2780    2937    3210    3887    5035    5280 
   Size:        446     578     150     157     273     677    1148     245

Cuts at:   5280    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     677     578     446     273     245     157
                 150
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5242
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5794
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5794
 
NotI GC'GGCC_GC

Cuts at:   3241    3241 
   Size:       5794
 
NspI r_CATG'y

Cuts at:   1132    1229    2389    2694    3152    4697    1132 
   Size:         97    1160     305     458    1545    2229

  Fragments arranged by size: 

                2229    1545    1160     458     305      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5794
 
PciI A'CATG_T

Cuts at:   2385    4693    2385 
   Size:       2308    3486
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5794
 
PfoI T'CCnGG_A

Cuts at:   2835    2835 
   Size:       5794
 
PmeI GTTT'AAAC

Cuts at:   3098    3098 
   Size:       5794
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3863    3895    5376    5408    3863 
   Size:         32    1481      32    4249

  Fragments arranged by size: 

                4249    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2225    2225 
   Size:       5794
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5794
 
PsiI TTA'TAA

Cuts at:   3364    3364 
   Size:       5794
 
PstI C_TGCA'G

Cuts at:    830    3146     830 
   Size:       2316    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5794
 
PvuII CAG'CTG

Cuts at:   2630    2780    2937    2630 
   Size:        150     157    5487

  Fragments arranged by size: 

                5487     157     150
 
SalI G'TCGA_C

Cuts at:   1936    3136    1936 
   Size:       1200    4594
 
SbfI CC_TGCA'GG

Cuts at:   3146    3146 
   Size:       5794
 
ScaI AGT'ACT

Cuts at:   4042    5754    4042 
   Size:       1712    4082
 
SfcI C'TryA_G

Cuts at:    826    1045    3142    4277    4958    5149     826 
   Size:        219    2097    1135     681     191    1471

  Fragments arranged by size: 

                2097    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5794
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5794
 
SmaI CCC'GGG

Cuts at:   1872    3121    1872 
   Size:       1249    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3898    4799    5061     820 
   Size:        197     926    1955     901     262    1553

  Fragments arranged by size: 

                1955    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3585     493 
   Size:       3092    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5794
 
SphI G_CATG'C

Cuts at:   1132    3152    1132 
   Size:       2020    3774
 
SspI AAT'ATT

Cuts at:      5      52    2191    2679    3718       5 
   Size:         47    2139     488    1039    2081

  Fragments arranged by size: 

                2139    2081    1039     488      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5794
 
StyI C'CwwG_G

Cuts at:    513    1065    2656    3025    3233     513 
   Size:        552    1591     369     208    3074

  Fragments arranged by size: 

                3074    1591     552     369     208
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2846    3821    3838    3991    4176    4621     325 
   Size:       1255    1266     975      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1266    1255     975     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4040    5752      96 
   Size:        274      80      33      51    1443    2063    1712     138

  Fragments arranged by size: 

                2063    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2740    2772    5754    5786    2740 
   Size:         32    2982      32    2748

  Fragments arranged by size: 

                2982    2748      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2762    1316 
   Size:       1446    4348
 
XbaI T'CTAG_A

Cuts at:   3130    3130 
   Size:       5794
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2627    2627 
   Size:       5794
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5794
 
XmaI C'CCGG_G

Cuts at:   1870    3119    1870 
   Size:       1249    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3923    3923 
   Size:       5794
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2554    3602     276 
   Size:         53      83     186     936    1020    1048    2468

  Fragments arranged by size: 

                2468    1048    1020     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
     PstI     PvuI    PvuII     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI
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