Restriction map for pFN21AB7156
 (Circular) (Six-base) MAPSORT of: pFN21AB7156.seq  Check: 7440  from: 1  to: 5782



pFN21AB7156  5782 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2456    2456 
   Size:       5782
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3592     278 
   Size:         53      83     186     936    2056    2468

  Fragments arranged by size: 

                2468    2056     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2262    3125    1937 
   Size:        325     863    4594

  Fragments arranged by size: 

                4594     863     325
 
Acc65I G'GTAC_C

Cuts at:   3103    3103 
   Size:       5782
 
AclI AA'CG_TT

Cuts at:   1563    3909    4282    1563 
   Size:       2346     373    3063

  Fragments arranged by size: 

                3063    2346     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2046    2201    2301    3845    5229    5497    1632 
   Size:        414     155     100    1544    1384     268    1917

  Fragments arranged by size: 

                1917    1544    1384     414     268     155     100
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5585
 
AflIII A'CryG_T

Cuts at:   1203    2363    4681    5658    1203 
   Size:       1160    2318     977    1327

  Fragments arranged by size: 

                2318    1327    1160     977
 
AgeI A'CCGG_T

Cuts at:   4613    4613 
   Size:       5782
 
AhdI GACnn_n'nnGTC

Cuts at:   4511    4511 
   Size:       5782
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5782
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5782
 
AlwNI CAG_nnn'CTG

Cuts at:   3525    5097    3525 
   Size:       1572    4210
 
ApaLI G'TGCA_C

Cuts at:   3839    4995    3839 
   Size:       1156    4626
 
ApoI r'AATT_y

Cuts at:   1493    2209    2566    2685    2927    3091    3317    3469    5578 
   Size:        716     357     119     242     164     226     152    2109

Cuts at:   5578    1493 
   Size:       1697

  Fragments arranged by size: 

                2109    1697     716     357     242     226     164     152
                 119
 
AseI AT'TA_AT

Cuts at:    160    1032    4336     160 
   Size:        872    3304    1606

  Fragments arranged by size: 

                3304    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5782
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3107    1870 
   Size:         73    1164    4545

  Fragments arranged by size: 

                4545    1164      73
 
BamHI G'GATC_C

Cuts at:   3112    3479    3112 
   Size:        367    5415
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3103    4558     618 
   Size:        325     217     627    1316    1455    1842

  Fragments arranged by size: 

                1842    1455    1316     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3101    1391 
   Size:       1710    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2189     928 
   Size:        971     290    4521

  Fragments arranged by size: 

                4521     971     290
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3973    4007    1903 
   Size:         34    2036      34    3678

  Fragments arranged by size: 

                3678    2036      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3674    4884    3674 
   Size:       1210    4572
 
BclI T'GATC_A

Cuts at:   1548    2631    1548 
   Size:       1083    4699
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4393     136 
   Size:        107     122      71     631     605    2721    1525

  Fragments arranged by size: 

                2721    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5777    5777 
   Size:       5782
 
BlpI GC'TnA_GC

Cuts at:   3199    3199 
   Size:       5782
 
Bme1580I G_kGCm'C

Cuts at:   1790    3843    4999    1790 
   Size:       2053    1156    2573

  Fragments arranged by size: 

                2573    2053    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5782
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2618    3513    4471 
   Size:        416      53      83     399      24    1201     895     958

Cuts at:   4471     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1201     958     895     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5782
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2930    4442    1239 
   Size:       1691    1512    2579

  Fragments arranged by size: 

                2579    1691    1512
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3907    4772    5070    3907 
   Size:        865     298    4619

  Fragments arranged by size: 

                4619     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4445     882 
   Size:        617    2946    2219

  Fragments arranged by size: 

                2946    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2364    2819    3573     493 
   Size:        711    1160     455     754    2702

  Fragments arranged by size: 

                2702    1160     754     711     455
 
BsaBI GATnn'nnATC

Cuts at:   5776    5776 
   Size:       5782
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3589    3971    4606     275 
   Size:         53      83     186     936    2056     382     635    1451

  Fragments arranged by size: 

                2056    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4215    4613    4887    5034    5445    4215 
   Size:        398     274     147     411    4552

  Fragments arranged by size: 

                4552     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5557

  Fragments arranged by size: 

                5557     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5782
 
BseYI C'CCAG_C

Cuts at:   2587    4985    5481    2587 
   Size:       2398     496    2888

  Fragments arranged by size: 

                2888    2398     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3045    1163 
   Size:        573    1309    3900

  Fragments arranged by size: 

                3900    1309     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3232    3993    5021    1540 
   Size:        275     186    1231     761    1028    2301

  Fragments arranged by size: 

                2301    1231    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3011    3843    3928    4999    3011 
   Size:        832      85    1071    3794

  Fragments arranged by size: 

                3794    1071     832      85
 
BsmI GAATG_Cn'

Cuts at:   3293    3386    3293 
   Size:         93    5689
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3011    3101    3843    3928    4999    1391 
   Size:        399    1221      90     742      85    1071    2174

  Fragments arranged by size: 

                2174    1221    1071     742     399      90      85
 
BspEI T'CCGG_A

Cuts at:   5445    5445 
   Size:       5782
 
BspHI T'CATG_A

Cuts at:   2091    3671    5401    2091 
   Size:       1580    1730    2472

  Fragments arranged by size: 

                2472    1730    1580
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2456    3137     844 
   Size:       1612     681    3489

  Fragments arranged by size: 

                3489    1612     681
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3669    1394 
   Size:        429    1846    3507

  Fragments arranged by size: 

                3507    1846     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4277    4451      65 
   Size:       1183    3029     174    1396

  Fragments arranged by size: 

                3029    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4426    4613    1700 
   Size:        110    2616     187    2869

  Fragments arranged by size: 

                2869    2616     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5782
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5782
 
BssSI C'ACGA_G

Cuts at:   3842    4854    3842 
   Size:       1012    4770
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5782
 
BstBI TT'CG_AA

Cuts at:   4586    4586 
   Size:       5782
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5782
 
BstYI r'GATC_y

Cuts at:   1972    3112    3479    3864    3881    5333    5419    5431    5777 
   Size:       1140     367     385      17    1452      86      12     346

Cuts at:   5777    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1140     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5230
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5043

  Fragments arranged by size: 

                5043     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3298    4092    4112    3298 
   Size:        794      20    4968

  Fragments arranged by size: 

                4968     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2380    1554 
   Size:        826    4956
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5747
 
DraI TTT'AAA

Cuts at:   2932    3086    2932 
   Size:        154    5628
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4789     809 
   Size:        507    3473    1802

  Fragments arranged by size: 

                3473    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2190    2478    2850 
   Size:         54    1425      50     275      18     360     288     372

Cuts at:   2850    3229    4118    5747       8 
   Size:        379     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     379     372     360     288     275
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3229    1537 
   Size:        275    1417    4090

  Fragments arranged by size: 

                4090    1417     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2023    3712    1353 
   Size:        670    1689    3423

  Fragments arranged by size: 

                3423    1689     670
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4369    4743    4889     387 
   Size:       1064     138    2780     374     146    1280

  Fragments arranged by size: 

                2780    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2046    2201    2301    2930    3845    4442    5229 
   Size:        393     414     155     100     629     915     597     787

Cuts at:   5229    5497    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     915     787     629     597     414     393     268
                 155     100
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2672    2672 
   Size:       5782
 
EcoO109I rG'GnC_Cy

Cuts at:   2665    2665 
   Size:       5782
 
EcoRI G'AATT_C

Cuts at:   3091    3469    3091 
   Size:        378    5404
 
FspI TGC'GCA

Cuts at:   4288    4288 
   Size:       5782
 
HaeII r_GCGC'y

Cuts at:   1678    4929    1678 
   Size:       3251    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2928    2960    4423    4455    4497 
   Size:         32     135      32    1356      32    1463      32      42

Cuts at:   4497    4529    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1463    1356     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3126    3372     669 
   Size:        988     281    1188     246    3079

  Fragments arranged by size: 

                3079    1188     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5782
 
HpaI GTT'AAC

Cuts at:   3372    3372 
   Size:       5782
 
KpnI G_GTAC'C

Cuts at:   3107    3107 
   Size:       5782
 
MfeI C'AATT_G

Cuts at:   2736    2887    3381    2736 
   Size:        151     494    5137

  Fragments arranged by size: 

                5137     494     151
 
MluI A'CGCG_T

Cuts at:   5658    5658 
   Size:       5782
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4896    5080    1305 
   Size:       3591     184    2007

  Fragments arranged by size: 

                3591    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2192    2480      10 
   Size:         54    1425     343     360     288    3312

  Fragments arranged by size: 

                3312    1425     360     343     288      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2120    2270    2505    2852    3740    4099    4258 
   Size:        648     954     150     235     347     888     359     159

Cuts at:   4258     518 
   Size:       2042

  Fragments arranged by size: 

                2042     954     888     648     359     347     235     159
                 150
 
MspA1I CmG'CkG

Cuts at:   1606    2283    3198    3875    5023    5268    1606 
   Size:        677     915     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     915     677     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5230
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5782
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5782
 
NotI GC'GGCC_GC

Cuts at:   3229    3229 
   Size:       5782
 
NspI r_CATG'y

Cuts at:   1132    1229    3140    4685    1132 
   Size:         97    1911    1545    2229

  Fragments arranged by size: 

                2229    1911    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5782
 
PciI A'CATG_T

Cuts at:   4681    4681 
   Size:       5782
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5782
 
PfoI T'CCnGG_A

Cuts at:   2759    2759 
   Size:       5782
 
PmeI GTTT'AAAC

Cuts at:   3086    3086 
   Size:       5782
 
PmlI CAC'GTG

Cuts at:   2364    2364 
   Size:       5782
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3851    3883    5364    5396    3851 
   Size:         32    1481      32    4237

  Fragments arranged by size: 

                4237    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5782
 
PsiI TTA'TAA

Cuts at:   3352    3352 
   Size:       5782
 
PstI C_TGCA'G

Cuts at:    830    3134     830 
   Size:       2304    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5782
 
SalI G'TCGA_C

Cuts at:   1936    3124    1936 
   Size:       1188    4594
 
SbfI CC_TGCA'GG

Cuts at:   3134    3134 
   Size:       5782
 
ScaI AGT'ACT

Cuts at:   4030    5742    4030 
   Size:       1712    4070
 
SfcI C'TryA_G

Cuts at:    826    1045    2824    3130    4265    4946    5137     826 
   Size:        219    1779     306    1135     681     191    1471

  Fragments arranged by size: 

                1779    1471    1135     681     306     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5782
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5782
 
SmaI CCC'GGG

Cuts at:   1872    3109    1872 
   Size:       1237    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3886    4787    5049     820 
   Size:        197     926    1943     901     262    1553

  Fragments arranged by size: 

                1943    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3573     493 
   Size:       3080    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5782
 
SphI G_CATG'C

Cuts at:   1132    3140    1132 
   Size:       2008    3774
 
SspI AAT'ATT

Cuts at:      5      52    2133    2533    2778    3706       5 
   Size:         47    2081     400     245     928    2081

  Fragments arranged by size: 

                2081    2081     928     400     245      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5782
 
StyI C'CwwG_G

Cuts at:    513    1065    3221     513 
   Size:        552    2156    3074

  Fragments arranged by size: 

                3074    2156     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2415    3809    3826    3979    4164    4609     325 
   Size:       1255     835    1394      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1394    1255     835     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4028    5740      96 
   Size:        274      80      33      51    1443    2051    1712     138

  Fragments arranged by size: 

                2051    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5742    5774    5742 
   Size:         32    5750
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5782
 
XbaI T'CTAG_A

Cuts at:   3118    3118 
   Size:       5782
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2605    2605 
   Size:       5782
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5782
 
XmaI C'CCGG_G

Cuts at:   1870    3107    1870 
   Size:       1237    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3911    3911 
   Size:       5782
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3590     276 
   Size:         53      83     186     936    2056    2468

  Fragments arranged by size: 

                2468    2056     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI     PstI     PvuI
     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI    PpuMI   PspOMI    PspXI
     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SapI    SexAI
     SfiI     SfoI     SrfI     SwaI
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