Restriction map for
pFN21AB7145
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsoBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssKI BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoRV EcoT14I EcoT22I FokI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HindIII HpaII HphI
KasI KpnI Ksp632I KspI MaeII
MamI MboII MflI MluI MroI
MspA1I MspI MvaI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
ScrFI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AciI 449 CCGC CCGC(-3/-1)
AflIII 296 ACATGT A^C[GA][CT]GT
AluI 16 AGCT AG^CT
Alw44I 443 GTGCAC G^TGCAC
ApaLI 443 GTGCAC G^TGCAC
Asp700I 508 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 443 GTGCAC G[AT]GC[AT]^C
BalI 244 TGGCCA TGG^CCA
BclI 704 TGATCA T^GATCA
BfaI 611 CTAG C^TAG
BmyI 443 GTGCAC G[AGT]GC[ACT]^C
BsgI 962 GTGCAG GTGCAG(16/14)
BsiHKAI 443 GTGCAC G[AT]GC[AT]^C
BsiYI 471 CCACTCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 471 CCACTCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 308 GCATTC GAATGC(1/-1)
Bsp1286I 443 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 696 TGTACA T^GTACA
BspDI 338 ATCGAT AT^CGAT
BspLU11I 296 ACATGT A^CATGT
BsrDI 178 GCAATG GCAATG(2/0)
BsrGI 696 TGTACA T^GTACA
Cac8I 499 GCCTGC GC[ACGT]^[ACGT]GC
ClaI 338 ATCGAT AT^CGAT
EaeI 244 TGGCCA [CT]^GGCC[GA]
EcoRI 897 GAATTC G^AATTC
FauI 448 CCCGC CCCGC(4/6)
FbaI 704 TGATCA T^GATCA
HaeIII 245 GGCC GG^CC
HgaI 131 GCGTC GACGC(5/10)
MaeI 611 CTAG C^TAG
MluNI 244 TGGCCA TGG^CCA
MscI 244 TGGCCA TGG^CCA
MslI 411 CACTGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
SfaNI 279 GATGC GCATC(5/9)
SmlI 543 CTCAAG C^T[CT][GA]AG
SspBI 696 TGTACA T^GTACA
Tsp45I 935 GTGAC ^GT[GC]AC
XcmI 517 CCAGAAGCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 508 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccI 355 GTCTAC GT^[AC][GT]AC
465 GTCTAC
BbvI 799 GCAGC GCAGC(8/12)
1027 GCTGC
BsrI 412 ACTGG ACTGG(1/-1)
917 ACTGG
DraI 594 TTTAAA TTT^AAA
681 TTTAAA
Fnu4HI 799 GCAGC GC^[ACGT]GC
1027 GCTGC
HincII 10 GTTAAC GT[CT]^[GA]AC
423 GTTAAC
HindII 10 GTTAAC GT[CT]^[GA]AC
423 GTTAAC
HpaI 10 GTTAAC GTT^AAC
423 GTTAAC
ItaI 799 GCAGC GC^[ACGT]GC
1027 GCTGC
MfeI 102 CAATTG C^AATTG
350 CAATTG
MunI 102 CAATTG C^AATTG
350 CAATTG
NspI 65 GCATGT [GA]CATG^[CT]
296 ACATGT
PleI 37 GACTC GAGTC(4/5)
375 GACTC
SfcI 863 CTATAG C^T[GA][CT]AG
988 CTACAG
TaqI 339 TCGA T^CGA
563 TCGA
TfiI 944 GATTC G^A[AT]TC
1021 GAATC
TseI 799 GCAGC G^C[AT]GC
1027 GCTGC
TspRI 22 CACTG CA[GC]TG[ACGT][ACGT]^
411 CACTG
TthHB8I 339 TCGA T^CGA
563 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AcsI AfaI ApoI DdeI DpnII
HinfI MaeIII MboI MnlI MseI
MwoI NdeII NlaIII RsaI Sau3AI
Tru9I Tsp509I