Restriction map for pFN21AB7145
 (Circular) (Six-base) MAPSORT of: pFN21AB7145.seq  Check: 7207  from: 1  to: 5737



pFN21AB7145  5737 bp

 With 182 enzymes: SgfI * 

                        March 28, 2012 14:41  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3547     278 
   Size:         53      83     186     936    2011    2468

  Fragments arranged by size: 

                2468    2011     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2361    2471    3080    1937 
   Size:        424     110     609    4594

  Fragments arranged by size: 

                4594     609     424     110
 
Acc65I G'GTAC_C

Cuts at:   3058    3058 
   Size:       5737
 
AclI AA'CG_TT

Cuts at:   1563    3864    4237    1563 
   Size:       2301     373    3063

  Fragments arranged by size: 

                3063    2301     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2203    2929    2976    3800    5184    5452    1632 
   Size:        571     726      47     824    1384     268    1917

  Fragments arranged by size: 

                1917    1384     824     726     571     268      47
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5540
 
AflIII A'CryG_T

Cuts at:   1203    2301    4636    5613    1203 
   Size:       1098    2335     977    1327

  Fragments arranged by size: 

                2335    1327    1098     977
 
AgeI A'CCGG_T

Cuts at:   4568    4568 
   Size:       5737
 
AhdI GACnn_n'nnGTC

Cuts at:   4466    4466 
   Size:       5737
 
AleI CACnn'nnGTG

Cuts at:   1166    2420    1166 
   Size:       1254    4483
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5737
 
AlwNI CAG_nnn'CTG

Cuts at:   3480    5052    3480 
   Size:       1572    4165
 
ApaLI G'TGCA_C

Cuts at:   2448    3794    4950    2448 
   Size:       1346    1156    3235

  Fragments arranged by size: 

                3235    1346    1156
 
ApoI r'AATT_y

Cuts at:   1493    2515    2902    2974    3046    3272    3424    5533    1493 
   Size:       1022     387      72      72     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1022     387     226     152      72      72
            
 
AseI AT'TA_AT

Cuts at:    160    1032    4291     160 
   Size:        872    3259    1606

  Fragments arranged by size: 

                3259    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5737
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3062    1870 
   Size:         73    1119    4545

  Fragments arranged by size: 

                4545    1119      73
 
BamHI G'GATC_C

Cuts at:   3067    3434    3067 
   Size:        367    5370
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3058    4513     618 
   Size:        325     217     627    1271    1455    1842

  Fragments arranged by size: 

                1842    1455    1271     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3056    1391 
   Size:       1665    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4766
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3928    3962    1903 
   Size:         34    1991      34    3678

  Fragments arranged by size: 

                3678    1991      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3629    4839    3629 
   Size:       1210    4527
 
BclI T'GATC_A

Cuts at:   1548    2709    1548 
   Size:       1161    4576
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4348     136 
   Size:        107     122      71     631     605    2676    1525

  Fragments arranged by size: 

                2676    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5732    5732 
   Size:       5737
 
BlpI GC'TnA_GC

Cuts at:   3154    3154 
   Size:       5737
 
Bme1580I G_kGCm'C

Cuts at:   1790    2452    3798    4954    1790 
   Size:        662    1346    1156    2573

  Fragments arranged by size: 

                2573    1346    1156     662
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5737
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3468    4426     442 
   Size:        416      53      83     399      24    2051     958    1753

  Fragments arranged by size: 

                2051    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5737
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4397    1239 
   Size:       3158    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2533    3862    4727    5025    2533 
   Size:       1329     865     298    3245

  Fragments arranged by size: 

                3245    1329     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4400     882 
   Size:        617    2901    2219

  Fragments arranged by size: 

                2901    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3528     493 
   Size:        711    2324    2702

  Fragments arranged by size: 

                2702    2324     711
 
BsaBI GATnn'nnATC

Cuts at:   5731    5731 
   Size:       5737
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3544    3926    4561     275 
   Size:         53      83     186     936    2011     382     635    1451

  Fragments arranged by size: 

                2011    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4170    4568    4842    4989    5400    4170 
   Size:        398     274     147     411    4507

  Fragments arranged by size: 

                4507     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5512

  Fragments arranged by size: 

                5512     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5737
 
BseYI C'CCAG_C

Cuts at:   4940    5436    4940 
   Size:        496    5241
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2988    1163 
   Size:        573    1252    3912

  Fragments arranged by size: 

                3912    1252     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3187    3948    4976    1540 
   Size:        275     186    1186     761    1028    2301

  Fragments arranged by size: 

                2301    1186    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2452    3798    3883    4954    2452 
   Size:       1346      85    1071    3235

  Fragments arranged by size: 

                3235    1346    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2313    3248    3341    2313 
   Size:        935      93    4709

  Fragments arranged by size: 

                4709     935      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2452    3056    3798    3883    4954    1391 
   Size:        399     662     604     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     662     604     399      85
 
BspEI T'CCGG_A

Cuts at:   5400    5400 
   Size:       5737
 
BspHI T'CATG_A

Cuts at:   3626    5356    3626 
   Size:       1730    4007
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3092     844 
   Size:       2248    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3624    1394 
   Size:        429    1801    3507

  Fragments arranged by size: 

                3507    1801     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2190    4232    4406      65 
   Size:       1183     942    2042     174    1396

  Fragments arranged by size: 

                2042    1396    1183     942     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4381    4568    1700 
   Size:        110    2571     187    2869

  Fragments arranged by size: 

                2869    2571     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2701      96 
   Size:       2605    3132
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5737
 
BssSI C'ACGA_G

Cuts at:   3797    4809    3797 
   Size:       1012    4725
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5737
 
BstBI TT'CG_AA

Cuts at:   4541    4541 
   Size:       5737
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5737
 
BstYI r'GATC_y

Cuts at:   1972    3067    3434    3819    3836    5288    5374    5386    5732 
   Size:       1095     367     385      17    1452      86      12     346

Cuts at:   5732    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452    1095     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4246

  Fragments arranged by size: 

                4246     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4998

  Fragments arranged by size: 

                4998     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2026    3253    4047    4067    2026 
   Size:       1227     794      20    3696

  Fragments arranged by size: 

                3696    1227     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2344    1554 
   Size:        790    4947
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5702
 
DraI TTT'AAA

Cuts at:   2601    2688    3041    2601 
   Size:         87     353    5297

  Fragments arranged by size: 

                5297     353      87
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4744     809 
   Size:        507    3428    1802

  Fragments arranged by size: 

                3428    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2249    3184    4073 
   Size:         54    1425      50     275      18     419     935     889

Cuts at:   4073    5702       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     935     889     419     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3184    1537 
   Size:        275    1372    4090

  Fragments arranged by size: 

                4090    1372     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3667    1353 
   Size:       2314    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4324    4698    4844     387 
   Size:       1064     138    2735     374     146    1280

  Fragments arranged by size: 

                2735    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2203    2929    2976    3800    4397    5184    5452 
   Size:        393     571     726      47     824     597     787     268

Cuts at:   5452    1239 
   Size:       1524

  Fragments arranged by size: 

                1524     824     787     726     597     571     393     268
                  47
 
EcoRI G'AATT_C

Cuts at:   2902    3046    3424    2902 
   Size:        144     378    5215

  Fragments arranged by size: 

                5215     378     144
 
FspI TGC'GCA

Cuts at:   4243    4243 
   Size:       5737
 
HaeII r_GCGC'y

Cuts at:   1678    4884    1678 
   Size:       3206    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3011    3043    4378    4410    4452 
   Size:         32     135      32    1439      32    1335      32      42

Cuts at:   4452    4484    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1439    1335     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2017    2430    3081    3327     669 
   Size:        988     281      79     413     651     246    3079

  Fragments arranged by size: 

                3079     988     651     413     281     246      79
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5737
 
HpaI GTT'AAC

Cuts at:   2017    2430    3327    2017 
   Size:        413     897    4427

  Fragments arranged by size: 

                4427     897     413
 
KpnI G_GTAC'C

Cuts at:   3062    3062 
   Size:       5737
 
MfeI C'AATT_G

Cuts at:   2107    2355    3336    2107 
   Size:        248     981    4508

  Fragments arranged by size: 

                4508     981     248
 
MluI A'CGCG_T

Cuts at:   5613    5613 
   Size:       5737
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2874    4851    5035    1305 
   Size:       1569    1977     184    2007

  Fragments arranged by size: 

                2007    1977    1569     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2251      10 
   Size:         54    1425     343     419    3496

  Fragments arranged by size: 

                3496    1425     419     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2420    3695    4054    4213     518 
   Size:        648    1254    1275     359     159    2042

  Fragments arranged by size: 

                2042    1275    1254     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3153    3830    4978    5223    1606 
   Size:       1547     677    1148     245    2120

  Fragments arranged by size: 

                2120    1547    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4246

  Fragments arranged by size: 

                4246     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5737
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5737
 
NotI GC'GGCC_GC

Cuts at:   3184    3184 
   Size:       5737
 
NspI r_CATG'y

Cuts at:   1132    1229    2074    2305    3095    4640    1132 
   Size:         97     845     231     790    1545    2229

  Fragments arranged by size: 

                2229    1545     845     790     231      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5737
 
PciI A'CATG_T

Cuts at:   2301    4636    2301 
   Size:       2335    3402
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5737
 
PmeI GTTT'AAAC

Cuts at:   3041    3041 
   Size:       5737
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3806    3838    5319    5351    3806 
   Size:         32    1481      32    4192

  Fragments arranged by size: 

                4192    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5737
 
PsiI TTA'TAA

Cuts at:   3307    3307 
   Size:       5737
 
PstI C_TGCA'G

Cuts at:    830    3089     830 
   Size:       2259    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5737
 
SalI G'TCGA_C

Cuts at:   1936    3079    1936 
   Size:       1143    4594
 
SbfI CC_TGCA'GG

Cuts at:   3089    3089 
   Size:       5737
 
ScaI AGT'ACT

Cuts at:   3985    5697    3985 
   Size:       1712    4025
 
SfcI C'TryA_G

Cuts at:    826    1045    2868    2993    3085    4220    4901    5092     826 
   Size:        219    1823     125      92    1135     681     191    1471

  Fragments arranged by size: 

                1823    1471    1135     681     219     191     125      92
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5737
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5737
 
SmaI CCC'GGG

Cuts at:   1872    3064    1872 
   Size:       1192    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2548    3841    4742    5004     820 
   Size:        197     926     605    1293     901     262    1553

  Fragments arranged by size: 

                1553    1293     926     901     605     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3528     493 
   Size:       3035    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5737
 
SphI G_CATG'C

Cuts at:   1132    3095    1132 
   Size:       1963    3774
 
SspI AAT'ATT

Cuts at:      5      52    3661       5 
   Size:         47    3609    2081

  Fragments arranged by size: 

                3609    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5737
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3176     513 
   Size:        552     939    1172    3074

  Fragments arranged by size: 

                3074    1172     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2055    3764    3781    3934    4119    4564     325 
   Size:       1255     475    1709      17     153     185     445    1498

  Fragments arranged by size: 

                1709    1498    1255     475     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2052    2701    3983 
   Size:        274      80      33      51    1443      75     649    1282

Cuts at:   3983    5695      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1282     649     274     138      80      75
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5697    5729    5697 
   Size:         32    5705
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5737
 
XbaI T'CTAG_A

Cuts at:   3073    3073 
   Size:       5737
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2529    2529 
   Size:       5737
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5737
 
XmaI C'CCGG_G

Cuts at:   1870    3062    1870 
   Size:       1192    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2517    3866    2517 
   Size:       1349    4388
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3545     276 
   Size:         53      83     186     936    2011    2468

  Fragments arranged by size: 

                2468    2011     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI
     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI
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