Restriction map for
pFN21AB7140
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BbeI BbrPI
BbvCI BciVI BclI BcnI BfaI
BfrI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Cac8I CelII CfoI ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TaqI Tsp45I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 803 GTAGAC GT^[AC][GT]AC
AciI 39 GCGG CCGC(-3/-1)
AcsI 904 AAATTC [GA]^AATT[CT]
AfaI 64 GTAC GT^AC
AocI 992 CCTTAGG CC^T[ACGT]AGG
ApoI 904 AAATTC [GA]^AATT[CT]
BamHI 873 GGATCC G^GATCC
BanII 340 GGGCTC G[GA]GC[CT]^C
BbsI 724 GTCTTC GAAGAC(2/6)
BcgI 493 GCAACACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 534 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 31 AGATCT A^GATCT
BpuAI 724 GTCTTC GAAGAC(2/6)
BsaJI 259 CCCTGG C^C[ACGT][ACGT]GG
BseRI 159 CTCCTC GAGGAG(10/8)
BsiYI 524 CCTTAAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 524 CCTTAAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrBI 37 GAGCGG CCGCTC(-3/-3)
BsrFI 106 ACCGGC [GA]^CCGG[CT]
BsrI 576 CCAGT ACTGG(1/-1)
BstXI 314 CCATGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 992 CCTTAGG CC^T[ACGT]AGG
Cfr10I 106 ACCGGC [GA]^CCGG[CT]
Cfr13I 532 GGGCC G^G[ACGT]CC
DraII 531 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 197 TGGCCG [CT]^GGCC[GA]
Eco81I 992 CCTTAGG CC^T[ACGT]AGG
EcoO109I 531 GGGGCCT [GA]G^G[ACGT]CC[CT]
HapII 107 CCGG C^CGG
HpaII 107 CCGG C^CGG
HphI 297 GGTGA GGTGA(8/7)
MspA1I 67 CAGCTG C[AC]G^C[GT]G
MspI 107 CCGG C^CGG
NdeI 570 CATATG CA^TATG
PleI 244 GACTC GAGTC(4/5)
PvuII 67 CAGCTG CAG^CTG
RsaI 64 GTAC GT^AC
Sau96I 532 GGGCC G^G[ACGT]CC
SfcI 801 CTGTAG C^T[GA][CT]AG
SspI 388 AATATT AAT^ATT
Tsp509I 905 AATT ^AATT
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwI 874 GATCC GGATC(4/5)
873 GGATC
BanI 41 GGTGCC G^G[CT][GA]CC
255 GGCACC
BbvI 264 GCAGC GCAGC(8/12)
853 GCAGC
BmyI 42 GTGCCC G[AGT]GC[ACT]^C
340 GGGCTC
BsaI 310 GAGACC GGTCTC(1/5)
423 GGTCTC
Bsp1286I 42 GTGCCC G[AGT]GC[ACT]^C
340 GGGCTC
BssKI 252 CCTGG ^CC[ACGT]GG
260 CCTGG
BstF5I 111 CATCC GGATG(2/0)
150 CATCC
BstNI 252 CCTGG CC^[AT]GG
260 CCTGG
BstYI 31 AGATCT [GA]^GATC[CT]
873 GGATCC
EcoRII 252 CCTGG ^CC[AT]GG
260 CCTGG
Fnu4HI 264 GCAGC GC^[ACGT]GC
853 GCAGC
FokI 111 CATCC GGATG(9/13)
150 CATCC
HaeIII 198 GGCC GG^CC
533 GGCC
ItaI 264 GCAGC GC^[ACGT]GC
853 GCAGC
MflI 31 AGATCT [GA]^GATC[CT]
873 GGATCC
MseI 526 TTAA T^TAA
880 TTAA
MvaI 252 CCTGG CC^[AT]GG
260 CCTGG
ScrFI 252 CCTGG CC^[ACGT]GG
260 CCTGG
SmlI 364 CTCAAG C^T[CT][GA]AG
477 CTTGAG
TfiI 359 GATTC G^A[AT]TC
646 GATTC
Tru9I 526 TTAA T^TAA
880 TTAA
TseI 264 GCAGC G^C[AT]GC
853 GCAGC
TspRI 11 CAGTG CA[GC]TG[ACGT][ACGT]^
92 CAGTG
XhoII 31 AGATCT [GA]^GATC[CT]
873 GGATCC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
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AluI BsmAI DdeI DpnII HinfI
MboI MboII MnlI MslI MwoI
NdeII NlaIII NlaIV Sau3AI SfaNI