Restriction map for
pFN21AB7139
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AlwI AlwNI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AvaI AvrII
BamHI BbrPI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SbfI ScaI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tsp509I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 271 GTCTAC GT^[AC][GT]AC
AccII 442 CGCG CG^CG
AfaI 528 GTAC GT^AC
AhdI 139 GACGAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 685 GTGCAC G^TGCAC
ApaI 814 GGGCCC GGGCC^C
ApaLI 685 GTGCAC G^TGCAC
AspEI 139 GACGAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 434 TGCGCA TGC^GCA
BbeI 521 GGCGCC GGCGC^C
BbvCI 705 CCTCAGC CCTCAGC(-5/-2)
BcnI 711 CCGGG CC^[GC]GG
BfaI 493 CTAG C^TAG
BsgI 180 CTGCAC GTGCAG(16/14)
BsmFI 965 GTCCC GGGAC(10/14)
BssSI 583 CTCGTG CACGAG(-5/-1)
BstUI 442 CGCG CG^CG
DrdI 589 GACTGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 383 CGGCCG C^GGCCG
Eam1105I 139 GACGAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 383 CGGCCG C^GGCCG
Eco52I 383 CGGCCG C^GGCCG
FspI 434 TGCGCA TGC^GCA
KasI 521 GGCGCC G^GCGCC
KspI 441 CCGCGG CCGC^GG
MaeI 493 CTAG C^TAG
MaeII 188 ACGT A^CGT
MseI 668 TTAA T^TAA
MspA1I 441 CCGCGG C[AC]G^C[GT]G
MvnI 442 CGCG CG^CG
NarI 521 GGCGCC GG^CGCC
NciI 711 CCGGG CC^[GC]GG
NcoI 539 CCATGG C^CATGG
PpuMI 356 AGGACCC [GA]G^G[AT]CC[CT]
PstI 238 CTGCAG CTGCA^G
RsaI 528 GTAC GT^AC
SacII 441 CCGCGG CCGC^GG
SfcI 238 CTGCAG C^T[GA][CT]AG
SfoI 521 GGCGCC GGC^GCC
StuI 203 AGGCCT AGG^CCT
Tru9I 668 TTAA T^TAA
XcmI 404 CCAAGATCTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 521 GGCGCC G[GA]^CG[CT]C
900 GGCGTC
AluI 335 AGCT AG^CT
973 AGCT
AspI 34 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
781 GACCAGGTC
BcgI 565 CGAGCACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
458 GCACCTATGTCG
BglI 113 GCCTGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
524 GCCTGTACGGC
BglII 68 AGATCT A^GATCT
407 AGATCT
BpmI 19 CTGGAG CTGGAG(16/14)
676 CTGGAG
BsaHI 521 GGCGCC G[GA]^CG[CT]C
900 GGCGTC
BseRI 762 CTCCTC GAGGAG(10/8)
889 GAGGAG
BsiEI 53 CGACCG CG[GA][CT]^CG
383 CGGCCG
BsmAI 297 GTCTC GTCTC(1/5)
598 GTCTC
BspMI 671 ACCTGC ACCTGC(4/8)
689 ACCTGC
BsrBI 310 GAGCGG CCGCTC(-3/-3)
370 GAGCGG
BsrFI 118 GCCGGC [GA]^CCGG[CT]
348 GCCGGC
BstYI 68 AGATCT [GA]^GATC[CT]
407 AGATCT
Cfr10I 118 GCCGGC [GA]^CCGG[CT]
348 GCCGGC
DpnII 69 GATC ^GATC
408 GATC
EcoT14I 353 CCAAGG C^C[AT][AT]GG
539 CCATGG
FauI 339 GCGGG CCCGC(4/6)
372 GCGGG
HaeII 375 GGCGCT [GA]GCGC^[CT]
521 GGCGCC
Hin1I 521 GGCGCC G[GA]^CG[CT]C
900 GGCGTC
HinfI 124 GAGTC G^A[ACGT]TC
650 GACTC
HphI 4 GGTGA GGTGA(8/7)
136 GGTGA
MboI 69 GATC ^GATC
408 GATC
MflI 68 AGATCT [GA]^GATC[CT]
407 AGATCT
MslI 630 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
636 CATGTTGGTG
NaeI 118 GCCGGC GCC^GGC
348 GCCGGC
NdeII 69 GATC ^GATC
408 GATC
NgoMIV 118 GCCGGC G^CCGGC
348 GCCGGC
PflFI 34 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
781 GACCAGGTC
PleI 650 GACTC GAGTC(4/5)
124 GAGTC
SapI 307 GAAGAGC GCTCTTC(1/4)
84 GCTCTTC
Sau3AI 69 GATC ^GATC
408 GATC
SexAI 35 ACCAGGT A^CC[AT]GGT
782 ACCAGGT
SfaNI 98 GCATC GCATC(5/9)
437 GCATC
StyI 353 CCAAGG C^C[AT][AT]GG
539 CCATGG
TaqI 52 TCGA T^CGA
155 TCGA
Tsp45I 5 GTGAC ^GT[GC]AC
137 GTGAC
Tth111I 34 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
781 GACCAGGTC
TthHB8I 52 TCGA T^CGA
155 TCGA
XhoII 68 AGATCT [GA]^GATC[CT]
407 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AspHI AvaII BalI BanI
BanII BbsI BbvI BmyI BpuAI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI Ksp632I MaeIII
MboII MluNI MnlI MscI MspI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI TseI TspRI