(Circular) (Six-base) MAPSORT of: pFN21AB7139.seq Check: 90 from: 1 to: 5704 pFN21AB7139 5704 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2703 2703 Size: 5704 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3514 278 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2277 3047 1937 Size: 340 770 4594 Fragments arranged by size: 4594 770 340 Acc65I G'GTAC_C Cuts at: 3025 3025 Size: 5704 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3767 5151 5419 1632 Size: 2135 1384 268 1917 Fragments arranged by size: 2135 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 4603 5580 1203 Size: 3400 977 1327 Fragments arranged by size: 3400 1327 977 AgeI A'CCGG_T Cuts at: 4535 4535 Size: 5704 AhdI GACnn_n'nnGTC Cuts at: 2149 4433 2149 Size: 2284 3420 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5704 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2510 1184 Size: 1326 4378 AlwNI CAG_nnn'CTG Cuts at: 3447 5019 3447 Size: 1572 4132 ApaI G_GGCC'C Cuts at: 2823 2823 Size: 5704 ApaLI G'TGCA_C Cuts at: 2690 3761 4917 2690 Size: 1071 1156 3477 Fragments arranged by size: 3477 1156 1071 ApoI r'AATT_y Cuts at: 1493 3013 3239 3391 5500 1493 Size: 1520 226 152 2109 1697 Fragments arranged by size: 2109 1697 1520 226 152 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 3029 1870 Size: 73 1086 4545 Fragments arranged by size: 4545 1086 73 BamHI G'GATC_C Cuts at: 3034 3401 3034 Size: 367 5337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2462 2526 2762 3025 4480 Size: 325 217 627 675 64 236 263 1455 Cuts at: 4480 618 Size: 1842 Fragments arranged by size: 1842 1455 675 627 325 263 236 217 64 BanII G_rGCy'C Cuts at: 1391 2553 2823 2856 3023 1391 Size: 1162 270 33 167 4072 Fragments arranged by size: 4072 1162 270 167 33 BbeI G_GCGC'C Cuts at: 2530 2530 Size: 5704 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2627 2785 2901 928 Size: 971 728 158 116 3731 Fragments arranged by size: 3731 971 728 158 116 BbvCI CC'TCA_GC Cuts at: 2711 2711 Size: 5704 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2452 2486 2559 2593 3895 3929 1903 Size: 34 515 34 73 34 1302 34 3678 Fragments arranged by size: 3678 1302 515 73 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 1548 Size: 5704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2124 2535 4315 Size: 107 122 71 631 605 452 411 1780 Cuts at: 4315 136 Size: 1525 Fragments arranged by size: 1780 1525 631 605 452 411 122 107 71 BglII A'GATC_T Cuts at: 2073 2412 5699 2073 Size: 339 3287 2078 Fragments arranged by size: 3287 2078 339 BlpI GC'TnA_GC Cuts at: 3121 3121 Size: 5704 Bme1580I G_kGCm'C Cuts at: 1790 2350 2465 2694 2731 2765 2823 3765 4921 Size: 560 115 229 37 34 58 942 1156 Cuts at: 4921 1790 Size: 2573 Fragments arranged by size: 2573 1156 942 560 229 115 58 37 34 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2023 2245 2884 Size: 416 53 83 399 24 606 222 639 Cuts at: 2884 3435 4393 442 Size: 551 958 1753 Fragments arranged by size: 1753 958 639 606 551 416 399 222 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5704 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2702 4364 1239 Size: 806 657 1662 2579 Fragments arranged by size: 2579 1662 806 657 Bpu10I CC'TnA_GC Cuts at: 2711 2711 Size: 5704 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3829 4694 4992 3829 Size: 865 298 4541 Fragments arranged by size: 4541 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4367 882 Size: 617 2868 2219 Fragments arranged by size: 2868 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3495 493 Size: 711 2291 2702 Fragments arranged by size: 2702 2291 711 BsaBI GATnn'nnATC Cuts at: 5698 5698 Size: 5704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2527 2906 3511 3893 Size: 53 83 186 936 994 379 605 382 Cuts at: 3893 4528 275 Size: 635 1451 Fragments arranged by size: 1451 994 936 635 605 382 379 186 83 53 BsaWI w'CCGG_w Cuts at: 4137 4535 4809 4956 5367 4137 Size: 398 274 147 411 4474 Fragments arranged by size: 4474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2540 2570 1178 Size: 30 165 30 1137 30 4312 Fragments arranged by size: 4312 1137 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2758 2909 1186 Size: 1572 151 3981 Fragments arranged by size: 3981 1572 151 BseYI C'CCAG_C Cuts at: 2175 2972 4907 5403 2175 Size: 797 1935 496 2476 Fragments arranged by size: 2476 1935 797 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2170 1163 Size: 573 434 4697 Fragments arranged by size: 4697 573 434 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 2391 3154 3915 4943 1540 Size: 275 186 60 330 763 761 1028 2301 Fragments arranged by size: 2301 1028 763 761 330 275 186 60 BsiHKAI G_wGCw'C Cuts at: 2055 2091 2455 2484 2575 2694 3765 3850 4921 Size: 36 364 29 91 119 1071 85 1071 Cuts at: 4921 2055 Size: 2838 Fragments arranged by size: 2838 1071 1071 364 119 91 85 36 29 BsmI GAATG_Cn' Cuts at: 3215 3308 3215 Size: 93 5611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2091 2350 2455 2465 2484 2553 Size: 399 265 36 259 105 10 19 69 Cuts at: 2553 2575 2694 2731 2765 2823 2856 3023 3765 Size: 22 119 37 34 58 33 167 742 Cuts at: 3765 3850 4921 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 265 259 167 119 105 85 69 58 37 36 34 33 22 19 10 BspEI T'CCGG_A Cuts at: 5367 5367 Size: 5704 BspHI T'CATG_A Cuts at: 3593 5323 3593 Size: 1730 3974 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2685 2703 3059 844 Size: 1841 18 356 3489 Fragments arranged by size: 3489 1841 356 18 BsrBI CCG'CTC Cuts at: 1394 1823 2317 2377 3591 1394 Size: 429 494 60 1214 3507 Fragments arranged by size: 3507 1214 494 429 60 BsrDI GCAATG_nn' Cuts at: 65 1248 4199 4373 65 Size: 1183 2951 174 1396 Fragments arranged by size: 2951 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2123 2353 4348 4535 1700 Size: 110 313 230 1995 187 2869 Fragments arranged by size: 2869 1995 313 230 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5704 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5704 BssSI C'ACGA_G Cuts at: 2588 3764 4776 2588 Size: 1176 1012 3516 Fragments arranged by size: 3516 1176 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5704 BstBI TT'CG_AA Cuts at: 4508 4508 Size: 5704 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5704 BstYI r'GATC_y Cuts at: 1972 2073 2412 3034 3401 3786 3803 5255 5341 Size: 101 339 622 367 385 17 1452 86 Cuts at: 5341 5353 5699 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 622 385 367 346 339 101 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2061 2446 2544 2613 2808 513 Size: 552 939 57 385 98 69 195 3409 Fragments arranged by size: 3409 939 552 385 195 98 69 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4965 Fragments arranged by size: 4965 683 56 BtsI GCAGTG_nn' Cuts at: 2348 3220 4014 4034 2348 Size: 872 794 20 4018 Fragments arranged by size: 4018 872 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5669 DraI TTT'AAA Cuts at: 3008 3008 Size: 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2600 4711 809 Size: 507 1284 2111 1802 Fragments arranged by size: 2111 1802 1284 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2355 2388 Size: 54 1425 50 275 18 291 234 33 Cuts at: 2388 2406 2518 2950 3151 4040 5669 8 Size: 18 112 432 201 889 1629 43 Fragments arranged by size: 1629 1425 889 432 291 275 234 201 112 54 50 43 33 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2388 3151 1537 Size: 275 576 763 4090 Fragments arranged by size: 4090 763 576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 2258 2307 2930 3634 1353 Size: 743 162 49 623 704 3423 Fragments arranged by size: 3423 743 704 623 162 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4291 4665 4811 387 Size: 1064 138 2702 374 146 1280 Fragments arranged by size: 2702 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2702 3767 4364 5151 5419 1239 Size: 393 413 657 1065 597 787 268 1524 Fragments arranged by size: 1524 1065 787 657 597 413 393 268 EcoO109I rG'GnC_Cy Cuts at: 2362 2819 2820 2916 2362 Size: 457 1 96 5150 Fragments arranged by size: 5150 457 96 1 EcoRI G'AATT_C Cuts at: 3013 3391 3013 Size: 378 5326 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2644 2644 Size: 5704 FspI TGC'GCA Cuts at: 2441 4210 2441 Size: 1769 3935 HaeII r_GCGC'y Cuts at: 1678 2384 2530 4851 1678 Size: 706 146 2321 2531 Fragments arranged by size: 2531 2321 706 146 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 4345 4377 4419 Size: 32 135 32 563 32 2178 32 42 Cuts at: 4419 4451 1373 Size: 32 2626 Fragments arranged by size: 2626 2178 563 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3048 3294 669 Size: 988 281 1110 246 3079 Fragments arranged by size: 3079 1110 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5704 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KasI G'GCGC_C Cuts at: 2526 2526 Size: 5704 KpnI G_GTAC'C Cuts at: 3029 3029 Size: 5704 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2632 4818 5002 1305 Size: 1327 2186 184 2007 Fragments arranged by size: 2186 2007 1327 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2408 2520 2952 10 Size: 54 1425 343 576 112 432 2762 Fragments arranged by size: 2762 1425 576 432 343 112 54 MslI CAynn'nnrTG Cuts at: 518 1166 2639 2645 3662 4021 4180 518 Size: 648 1473 6 1017 359 159 2042 Fragments arranged by size: 2042 1473 1017 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2448 3120 3797 4945 5190 1606 Size: 842 672 677 1148 245 2120 Fragments arranged by size: 2120 1148 842 677 672 245 NaeI GCC'GGC Cuts at: 2125 2355 2125 Size: 230 5474 NarI GG'CG_CC Cuts at: 2527 2527 Size: 5704 NcoI C'CATG_G Cuts at: 513 1065 2004 2544 513 Size: 552 939 540 3673 Fragments arranged by size: 3673 939 552 540 NdeI CA'TA_TG Cuts at: 387 387 Size: 5704 NgoMIV G'CCGG_C Cuts at: 2123 2353 2123 Size: 230 5474 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5704 NotI GC'GGCC_GC Cuts at: 3151 3151 Size: 5704 NspI r_CATG'y Cuts at: 1132 1229 3062 4607 1132 Size: 97 1833 1545 2229 Fragments arranged by size: 2229 1833 1545 97 PasI CC'CwG_GG Cuts at: 1348 2758 1348 Size: 1410 4294 PciI A'CATG_T Cuts at: 4603 4603 Size: 5704 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5704 PfoI T'CCnGG_A Cuts at: 2022 2046 2928 2022 Size: 24 882 4798 Fragments arranged by size: 4798 882 24 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3773 3805 5286 5318 3773 Size: 32 1481 32 4159 Fragments arranged by size: 4159 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2362 2362 Size: 5704 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 3274 3274 Size: 5704 PspOMI G'GGCC_C Cuts at: 2819 2819 Size: 5704 PstI C_TGCA'G Cuts at: 830 2247 3056 830 Size: 1417 809 3478 Fragments arranged by size: 3478 1417 809 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 SacII CC_GC'GG Cuts at: 2449 2449 Size: 5704 SalI G'TCGA_C Cuts at: 1936 3046 1936 Size: 1110 4594 SapI GCTCTTCn'nnn_ Cuts at: 2096 2307 2096 Size: 211 5493 SbfI CC_TGCA'GG Cuts at: 3056 3056 Size: 5704 ScaI AGT'ACT Cuts at: 3952 5664 3952 Size: 1712 3992 SexAI A'CCwGG_T Cuts at: 2040 2787 2040 Size: 747 4957 SfcI C'TryA_G Cuts at: 826 1045 2243 3052 4187 4868 5059 826 Size: 219 1198 809 1135 681 191 1471 Fragments arranged by size: 1471 1198 1135 809 681 219 191 SfoI GGC'GCC Cuts at: 2528 2528 Size: 5704 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5704 SmaI CCC'GGG Cuts at: 1872 3031 1872 Size: 1159 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3808 4709 4971 820 Size: 197 926 1865 901 262 1553 Fragments arranged by size: 1865 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3495 493 Size: 3002 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5704 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SspI AAT'ATT Cuts at: 5 52 3628 5 Size: 47 3576 2081 Fragments arranged by size: 3576 2081 47 StuI AGG'CCT Cuts at: 1517 2210 1517 Size: 693 5011 StyI C'CwwG_G Cuts at: 513 1065 2004 2358 2544 3143 513 Size: 552 939 354 186 599 3074 Fragments arranged by size: 3074 939 599 552 354 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2227 2281 3731 3748 3901 4086 Size: 1255 417 230 54 1450 17 153 185 Cuts at: 4086 4531 325 Size: 445 1498 Fragments arranged by size: 1498 1450 1255 445 417 230 185 153 54 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3950 5662 96 Size: 274 80 33 51 1443 1973 1712 138 Fragments arranged by size: 1973 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5664 5696 5664 Size: 32 5672 Tth111I GACn'n_nGTC Cuts at: 1316 2042 2789 1316 Size: 726 747 4231 Fragments arranged by size: 4231 747 726 XbaI T'CTAG_A Cuts at: 3040 3040 Size: 5704 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2416 2416 Size: 5704 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5704 XmaI C'CCGG_G Cuts at: 1870 3029 1870 Size: 1159 4545 XmnI GAAnn'nnTTC Cuts at: 3833 3833 Size: 5704 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3512 276 Size: 53 83 186 936 1978 2468 Fragments arranged by size: 2468 1978 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SfiI SrfI SwaI