(Circular) (Six-base) MAPSORT of: pFN21AB7119.seq Check: 9267 from: 1 to: 5566 pFN21AB7119 5566 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2779 2779 Size: 5566 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3376 278 Size: 53 83 186 936 1840 2468 Fragments arranged by size: 2468 1840 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2763 2909 1937 Size: 826 146 4594 Fragments arranged by size: 4594 826 146 Acc65I G'GTAC_C Cuts at: 2887 2887 Size: 5566 AclI AA'CG_TT Cuts at: 1563 3693 4066 1563 Size: 2130 373 3063 Fragments arranged by size: 3063 2130 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3629 5013 5281 1632 Size: 1997 1384 268 1917 Fragments arranged by size: 1997 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5369 AflIII A'CryG_T Cuts at: 1203 2541 2565 4465 5442 1203 Size: 1338 24 1900 977 1327 Fragments arranged by size: 1900 1338 1327 977 24 AgeI A'CCGG_T Cuts at: 4397 4397 Size: 5566 AhdI GACnn_n'nnGTC Cuts at: 4295 4295 Size: 5566 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5566 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5566 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2090 2122 2090 Size: 32 5534 AlwNI CAG_nnn'CTG Cuts at: 2176 3309 4881 2176 Size: 1133 1572 2861 Fragments arranged by size: 2861 1572 1133 ApaLI G'TGCA_C Cuts at: 3623 4779 3623 Size: 1156 4410 ApoI r'AATT_y Cuts at: 1493 2875 3101 3253 5362 1493 Size: 1382 226 152 2109 1697 Fragments arranged by size: 2109 1697 1382 226 152 AseI AT'TA_AT Cuts at: 160 1032 4120 160 Size: 872 3088 1606 Fragments arranged by size: 3088 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5566 AvaI C'yCGr_G Cuts at: 1870 1943 2289 2521 2891 1870 Size: 73 346 232 370 4545 Fragments arranged by size: 4545 370 346 232 73 BamHI G'GATC_C Cuts at: 2896 3263 2896 Size: 367 5199 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2235 2887 4342 618 Size: 325 217 627 448 652 1455 1842 Fragments arranged by size: 1842 1455 652 627 448 325 217 BanII G_rGCy'C Cuts at: 1391 2685 2745 2885 1391 Size: 1294 60 140 4072 Fragments arranged by size: 4072 1294 140 60 BbeI G_GCGC'C Cuts at: 2239 2239 Size: 5566 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4595 BbvCI CC'TCA_GC Cuts at: 2240 2346 2240 Size: 106 5460 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3757 3791 1903 Size: 34 1820 34 3678 Fragments arranged by size: 3678 1820 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3458 4668 3458 Size: 1210 4356 BclI T'GATC_A Cuts at: 1548 1548 Size: 5566 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2285 4177 136 Size: 107 122 71 631 605 613 1892 1525 Fragments arranged by size: 1892 1525 631 613 605 122 107 71 BglII A'GATC_T Cuts at: 5561 5561 Size: 5566 BlpI GC'TnA_GC Cuts at: 2333 2983 2333 Size: 650 4916 Bme1580I G_kGCm'C Cuts at: 1790 3627 4783 1790 Size: 1837 1156 2573 Fragments arranged by size: 2573 1837 1156 BmgBI CAC'GTC Cuts at: 1318 2188 2226 2566 2593 1318 Size: 870 38 340 27 4291 Fragments arranged by size: 4291 870 340 38 27 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3297 4255 442 Size: 416 53 83 399 24 1880 958 1753 Fragments arranged by size: 1880 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2021 1056 Size: 965 4601 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2101 2101 Size: 5566 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2519 2675 4226 1239 Size: 908 372 156 1551 2579 Fragments arranged by size: 2579 1551 908 372 156 Bpu10I CC'TnA_GC Cuts at: 2240 2346 2240 Size: 106 5460 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2846 3691 4556 4854 2846 Size: 845 865 298 3558 Fragments arranged by size: 3558 865 845 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4229 882 Size: 617 2730 2219 Fragments arranged by size: 2730 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3357 493 Size: 711 2153 2702 Fragments arranged by size: 2702 2153 711 BsaBI GATnn'nnATC Cuts at: 2483 5560 2483 Size: 3077 2489 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2236 3373 3755 4390 Size: 53 83 186 936 703 1137 382 635 Cuts at: 4390 275 Size: 1451 Fragments arranged by size: 1451 1137 936 703 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3999 4397 4671 4818 5229 3999 Size: 398 274 147 411 4336 Fragments arranged by size: 4336 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2150 2249 2279 1178 Size: 30 165 30 717 30 99 30 4465 Fragments arranged by size: 4465 717 165 99 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2828 1186 Size: 1642 3924 BseYI C'CCAG_C Cuts at: 2197 2352 4769 5265 2197 Size: 155 2417 496 2498 Fragments arranged by size: 2498 2417 496 155 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2825 1163 Size: 573 1089 3904 Fragments arranged by size: 3904 1089 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2763 3016 3777 4805 1540 Size: 275 186 762 253 761 1028 2301 Fragments arranged by size: 2301 1028 762 761 275 253 186 BsiHKAI G_wGCw'C Cuts at: 2685 2745 3627 3712 4783 2685 Size: 60 882 85 1071 3468 Fragments arranged by size: 3468 1071 882 85 60 BsmI GAATG_Cn' Cuts at: 3077 3170 3077 Size: 93 5473 BsmBI CGTCTCn'nnnn_ Cuts at: 2105 2105 Size: 5566 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2685 2745 2885 3627 3712 4783 1391 Size: 399 895 60 140 742 85 1071 2174 Fragments arranged by size: 2174 1071 895 742 399 140 85 60 BspEI T'CCGG_A Cuts at: 5229 5229 Size: 5566 BspHI T'CATG_A Cuts at: 3455 5185 3455 Size: 1730 3836 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2779 2921 844 Size: 1935 142 3489 Fragments arranged by size: 3489 1935 142 BsrBI CCG'CTC Cuts at: 1394 1823 3453 1394 Size: 429 1630 3507 Fragments arranged by size: 3507 1630 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4061 4235 65 Size: 1183 2813 174 1396 Fragments arranged by size: 2813 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2013 2771 4210 4397 1700 Size: 110 203 758 1439 187 2869 Fragments arranged by size: 2869 1439 758 203 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5566 BssHII G'CGCG_C Cuts at: 1925 2317 2378 2425 2601 1925 Size: 392 61 47 176 4890 Fragments arranged by size: 4890 392 176 61 47 BssSI C'ACGA_G Cuts at: 2093 2678 3626 4638 2093 Size: 585 948 1012 3021 Fragments arranged by size: 3021 1012 948 585 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5566 BstBI TT'CG_AA Cuts at: 4370 4370 Size: 5566 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5566 BstYI r'GATC_y Cuts at: 1972 2478 2896 3263 3648 3665 5117 5203 5215 Size: 506 418 367 385 17 1452 86 12 Cuts at: 5215 5561 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 506 418 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5014 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2254 487 Size: 683 56 1028 3799 Fragments arranged by size: 3799 1028 683 56 BtsI GCAGTG_nn' Cuts at: 2305 2379 3082 3876 3896 2305 Size: 74 703 794 20 3975 Fragments arranged by size: 3975 794 703 74 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5566 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5531 DraI TTT'AAA Cuts at: 2870 2870 Size: 5566 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4573 809 Size: 507 3257 1802 Fragments arranged by size: 3257 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 2586 2773 Size: 54 1425 50 275 18 351 405 187 Cuts at: 2773 3013 3902 5531 8 Size: 240 889 1629 43 Fragments arranged by size: 1629 1425 889 405 351 275 240 187 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3013 1537 Size: 275 1201 4090 Fragments arranged by size: 4090 1201 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2690 3496 1353 Size: 1337 806 3423 Fragments arranged by size: 3423 1337 806 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4153 4527 4673 387 Size: 1064 138 2564 374 146 1280 Fragments arranged by size: 2564 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2683 2743 2683 Size: 60 5506 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2519 2675 3629 4226 5013 5281 Size: 393 515 372 156 954 597 787 268 Cuts at: 5281 1239 Size: 1524 Fragments arranged by size: 1524 954 787 597 515 393 372 268 156 EcoRI G'AATT_C Cuts at: 2875 3253 2875 Size: 378 5188 FspI TGC'GCA Cuts at: 4072 4072 Size: 5566 HaeII r_GCGC'y Cuts at: 1678 2239 2494 4713 1678 Size: 561 255 2219 2531 Fragments arranged by size: 2531 2219 561 255 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2249 2281 4207 4239 4281 Size: 32 135 32 677 32 1926 32 42 Cuts at: 4281 4313 1373 Size: 32 2626 Fragments arranged by size: 2626 1926 677 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2764 2910 3156 669 Size: 988 281 826 146 246 3079 Fragments arranged by size: 3079 988 826 281 246 146 HindIII A'AGCT_T Cuts at: 748 748 Size: 5566 HpaI GTT'AAC Cuts at: 3156 3156 Size: 5566 KasI G'GCGC_C Cuts at: 2235 2235 Size: 5566 KpnI G_GTAC'C Cuts at: 2891 2891 Size: 5566 MfeI C'AATT_G Cuts at: 3165 3165 Size: 5566 MluI A'CGCG_T Cuts at: 5442 5442 Size: 5566 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4680 4864 1305 Size: 3375 184 2007 Fragments arranged by size: 3375 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3744 Fragments arranged by size: 3744 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2228 3524 3883 4042 518 Size: 648 1062 1296 359 159 2042 Fragments arranged by size: 2042 1296 1062 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2173 2497 2982 3659 4807 5052 1606 Size: 567 324 485 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 567 485 324 245 NarI GG'CG_CC Cuts at: 2236 2236 Size: 5566 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5014 NdeI CA'TA_TG Cuts at: 387 387 Size: 5566 NheI G'CTAG_C Cuts at: 1052 2017 1052 Size: 965 4601 NotI GC'GGCC_GC Cuts at: 3013 3013 Size: 5566 NspI r_CATG'y Cuts at: 1132 1229 2545 2924 4469 1132 Size: 97 1316 379 1545 2229 Fragments arranged by size: 2229 1545 1316 379 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5566 PciI A'CATG_T Cuts at: 2541 4465 2541 Size: 1924 3642 PflMI CCAn_nnn'nTGG Cuts at: 1108 2125 2176 1108 Size: 1017 51 4498 Fragments arranged by size: 4498 1017 51 PfoI T'CCnGG_A Cuts at: 2124 2148 2124 Size: 24 5542 PmeI GTTT'AAAC Cuts at: 2870 2870 Size: 5566 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2528 2560 3635 3667 5148 5180 2528 Size: 32 1075 32 1481 32 2914 Fragments arranged by size: 2914 1481 1075 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5566 PsiI TTA'TAA Cuts at: 3136 3136 Size: 5566 PstI C_TGCA'G Cuts at: 830 2918 830 Size: 2088 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5566 PvuII CAG'CTG Cuts at: 2173 2497 2173 Size: 324 5242 SacI G_AGCT'C Cuts at: 2685 2745 2685 Size: 60 5506 SalI G'TCGA_C Cuts at: 1936 2762 2908 1936 Size: 826 146 4594 Fragments arranged by size: 4594 826 146 SapI GCTCTTCn'nnn_ Cuts at: 2690 2690 Size: 5566 SbfI CC_TGCA'GG Cuts at: 2918 2918 Size: 5566 ScaI AGT'ACT Cuts at: 3814 5526 3814 Size: 1712 3854 SfcI C'TryA_G Cuts at: 826 1045 2914 4049 4730 4921 826 Size: 219 1869 1135 681 191 1471 Fragments arranged by size: 1869 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2285 2285 Size: 5566 SfoI GGC'GCC Cuts at: 2237 2237 Size: 5566 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5566 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5566 SmaI CCC'GGG Cuts at: 1872 2291 2893 1872 Size: 419 602 4545 Fragments arranged by size: 4545 602 419 SmlI C'TyrA_G Cuts at: 820 1017 1943 2861 3670 4571 4833 820 Size: 197 926 918 809 901 262 1553 Fragments arranged by size: 1553 926 918 901 809 262 197 SnaBI TAC'GTA Cuts at: 493 3357 493 Size: 2864 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5566 SphI G_CATG'C Cuts at: 1132 2924 1132 Size: 1792 3774 SspI AAT'ATT Cuts at: 5 52 3490 5 Size: 47 3438 2081 Fragments arranged by size: 3438 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5566 StyI C'CwwG_G Cuts at: 513 1065 2274 2781 3005 513 Size: 552 1209 507 224 3074 Fragments arranged by size: 3074 1209 552 507 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3593 3610 3763 3948 4393 325 Size: 1255 2013 17 153 185 445 1498 Fragments arranged by size: 2013 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2397 3812 5524 Size: 274 80 33 51 1443 420 1415 1712 Cuts at: 5524 96 Size: 138 Fragments arranged by size: 1712 1443 1415 420 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5526 5558 5526 Size: 32 5534 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5566 XbaI T'CTAG_A Cuts at: 2902 2902 Size: 5566 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5566 XmaI C'CCGG_G Cuts at: 1870 2289 2891 1870 Size: 419 602 4545 Fragments arranged by size: 4545 602 419 XmnI GAAnn'nnTTC Cuts at: 3695 3695 Size: 5566 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3374 276 Size: 53 83 186 936 1840 2468 Fragments arranged by size: 2468 1840 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI XcmI