(Circular) (Six-base) MAPSORT of: pFN21AB7113.seq Check: 5194 from: 1 to: 5515 pFN21AB7113 5515 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2726 2726 Size: 5515 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3325 278 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2109 2858 1937 Size: 172 749 4594 Fragments arranged by size: 4594 749 172 Acc65I G'GTAC_C Cuts at: 2836 2836 Size: 5515 AclI AA'CG_TT Cuts at: 1563 3642 4015 1563 Size: 2079 373 3063 Fragments arranged by size: 3063 2079 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2775 3578 4962 5230 1632 Size: 1143 803 1384 268 1917 Fragments arranged by size: 1917 1384 1143 803 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5318 AflIII A'CryG_T Cuts at: 1203 4414 5391 1203 Size: 3211 977 1327 Fragments arranged by size: 3211 1327 977 AgeI A'CCGG_T Cuts at: 4346 4346 Size: 5515 AhdI GACnn_n'nnGTC Cuts at: 4244 4244 Size: 5515 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5515 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5515 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2036 2068 2036 Size: 32 5483 AlwNI CAG_nnn'CTG Cuts at: 3258 4830 3258 Size: 1572 3943 ApaI G_GGCC'C Cuts at: 2239 2239 Size: 5515 ApaLI G'TGCA_C Cuts at: 3572 4728 3572 Size: 1156 4359 ApoI r'AATT_y Cuts at: 1493 2824 3050 3202 5311 1493 Size: 1331 226 152 2109 1697 Fragments arranged by size: 2109 1697 1331 226 152 AseI AT'TA_AT Cuts at: 160 1032 4069 160 Size: 872 3037 1606 Fragments arranged by size: 3037 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 AvaI C'yCGr_G Cuts at: 1870 1943 2673 2840 1870 Size: 73 730 167 4545 Fragments arranged by size: 4545 730 167 73 BamHI G'GATC_C Cuts at: 2758 2845 3212 2758 Size: 87 367 5061 Fragments arranged by size: 5061 367 87 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2316 2582 2836 4291 618 Size: 325 217 627 529 266 254 1455 1842 Fragments arranged by size: 1842 1455 627 529 325 266 254 217 BanII G_rGCy'C Cuts at: 1391 2239 2622 2834 1391 Size: 848 383 212 4072 Fragments arranged by size: 4072 848 383 212 BbeI G_GCGC'C Cuts at: 2586 2586 Size: 5515 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2021 2086 928 Size: 971 122 65 4357 Fragments arranged by size: 4357 971 122 65 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2664 2698 3706 3740 1903 Size: 34 727 34 1008 34 3678 Fragments arranged by size: 3678 1008 727 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3407 4617 3407 Size: 1210 4305 BclI T'GATC_A Cuts at: 1548 1548 Size: 5515 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2504 4126 136 Size: 107 122 71 631 605 832 1622 1525 Fragments arranged by size: 1622 1525 832 631 605 122 107 71 BglII A'GATC_T Cuts at: 5510 5510 Size: 5515 BlpI GC'TnA_GC Cuts at: 2932 2932 Size: 5515 Bme1580I G_kGCm'C Cuts at: 1790 2239 3576 4732 1790 Size: 449 1337 1156 2573 Fragments arranged by size: 2573 1337 1156 449 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5515 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3246 4204 442 Size: 416 53 83 399 24 1829 958 1753 Fragments arranged by size: 1829 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2006 4175 1239 Size: 767 2169 2579 Fragments arranged by size: 2579 2169 767 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3640 4505 4803 3640 Size: 865 298 4352 Fragments arranged by size: 4352 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4178 882 Size: 617 2679 2219 Fragments arranged by size: 2679 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2113 2287 3306 493 Size: 711 909 174 1019 2702 Fragments arranged by size: 2702 1019 909 711 174 BsaBI GATnn'nnATC Cuts at: 5509 5509 Size: 5515 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2583 3322 3704 4339 Size: 53 83 186 936 1050 739 382 635 Cuts at: 4339 275 Size: 1451 Fragments arranged by size: 1451 1050 936 739 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3948 4346 4620 4767 5178 3948 Size: 398 274 147 411 4285 Fragments arranged by size: 4285 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2036 2066 1178 Size: 30 165 30 633 30 4627 Fragments arranged by size: 4627 633 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2315 1186 Size: 1129 4386 BseYI C'CCAG_C Cuts at: 2747 4718 5214 2747 Size: 1971 496 3048 Fragments arranged by size: 3048 1971 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2167 2267 2435 2510 2642 1163 Size: 573 431 100 168 75 132 4036 Fragments arranged by size: 4036 573 431 168 132 100 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2965 3726 4754 1540 Size: 275 186 964 761 1028 2301 Fragments arranged by size: 2301 1028 964 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2622 3576 3661 4732 2622 Size: 954 85 1071 3405 Fragments arranged by size: 3405 1071 954 85 BsmI GAATG_Cn' Cuts at: 3026 3119 3026 Size: 93 5422 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2239 2622 2834 3576 3661 4732 1391 Size: 399 449 383 212 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 449 399 383 212 85 BspEI T'CCGG_A Cuts at: 5178 5178 Size: 5515 BspHI T'CATG_A Cuts at: 3404 5134 3404 Size: 1730 3785 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2454 2726 2870 844 Size: 1610 272 144 3489 Fragments arranged by size: 3489 1610 272 144 BsrBI CCG'CTC Cuts at: 1394 1823 2037 2305 3402 1394 Size: 429 214 268 1097 3507 Fragments arranged by size: 3507 1097 429 268 214 BsrDI GCAATG_nn' Cuts at: 65 1248 2535 4010 4184 65 Size: 1183 1287 1475 174 1396 Fragments arranged by size: 1475 1396 1287 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2313 4159 4346 1700 Size: 110 503 1846 187 2869 Fragments arranged by size: 2869 1846 503 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5515 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5515 BssSI C'ACGA_G Cuts at: 3575 4587 3575 Size: 1012 4503 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5515 BstBI TT'CG_AA Cuts at: 4319 4319 Size: 5515 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5515 BstXI CCAn_nnnn'nTGG Cuts at: 2524 2524 Size: 5515 BstYI r'GATC_y Cuts at: 1972 2758 2845 3212 3597 3614 5066 5152 5164 Size: 786 87 367 385 17 1452 86 12 Cuts at: 5164 5510 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 786 385 367 346 87 86 17 12 BstZ17I GTA'TAC Cuts at: 2110 2110 Size: 5515 BtgI C'CryG_G Cuts at: 513 1065 2004 2082 513 Size: 552 939 78 3946 Fragments arranged by size: 3946 939 552 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4776 Fragments arranged by size: 4776 683 56 BtsI GCAGTG_nn' Cuts at: 2498 3031 3825 3845 2498 Size: 533 794 20 4168 Fragments arranged by size: 4168 794 533 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5480 DraI TTT'AAA Cuts at: 2819 2819 Size: 5515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4522 809 Size: 507 3206 1802 Fragments arranged by size: 3206 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2962 3851 5480 Size: 54 1425 50 275 18 1132 889 1629 Cuts at: 5480 8 Size: 43 Fragments arranged by size: 1629 1425 1132 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2962 1537 Size: 275 1150 4090 Fragments arranged by size: 4090 1150 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2441 3445 1353 Size: 1088 1004 3423 Fragments arranged by size: 3423 1088 1004 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2216 2578 2689 4102 4476 4622 Size: 1064 138 627 362 111 1413 374 146 Cuts at: 4622 387 Size: 1280 Fragments arranged by size: 1413 1280 1064 627 374 362 146 138 111 EcoICRI GAG'CTC Cuts at: 2620 2620 Size: 5515 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2006 2775 3578 4175 4962 5230 1239 Size: 393 374 769 803 597 787 268 1524 Fragments arranged by size: 1524 803 787 769 597 393 374 268 EcoNI CCTnn'n_nnAGG Cuts at: 2660 2660 Size: 5515 EcoO109I rG'GnC_Cy Cuts at: 2026 2236 2644 2026 Size: 210 408 4897 Fragments arranged by size: 4897 408 210 EcoRI G'AATT_C Cuts at: 2824 3202 2824 Size: 378 5137 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2578 2578 Size: 5515 FspI TGC'GCA Cuts at: 4021 4021 Size: 5515 HaeII r_GCGC'y Cuts at: 1678 2314 2586 4662 1678 Size: 636 272 2076 2531 Fragments arranged by size: 2531 2076 636 272 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4156 4188 4230 4262 1373 Size: 32 135 32 2584 32 42 32 2626 Fragments arranged by size: 2626 2584 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2859 3105 669 Size: 988 281 921 246 3079 Fragments arranged by size: 3079 988 921 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5515 HpaI GTT'AAC Cuts at: 3105 3105 Size: 5515 KasI G'GCGC_C Cuts at: 2582 2582 Size: 5515 KpnI G_GTAC'C Cuts at: 2840 2840 Size: 5515 MfeI C'AATT_G Cuts at: 3114 3114 Size: 5515 MluI A'CGCG_T Cuts at: 5391 5391 Size: 5515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2244 4629 4813 1305 Size: 939 2385 184 2007 Fragments arranged by size: 2385 2007 939 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3693 Fragments arranged by size: 3693 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3473 3832 3991 518 Size: 648 2307 359 159 2042 Fragments arranged by size: 2307 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2724 2931 3608 4756 5001 1606 Size: 1118 207 677 1148 245 2120 Fragments arranged by size: 2120 1148 1118 677 245 207 NaeI GCC'GGC Cuts at: 2315 2315 Size: 5515 NarI GG'CG_CC Cuts at: 2583 2583 Size: 5515 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4024 Fragments arranged by size: 4024 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5515 NgoMIV G'CCGG_C Cuts at: 2313 2313 Size: 5515 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5515 NotI GC'GGCC_GC Cuts at: 2962 2962 Size: 5515 NspI r_CATG'y Cuts at: 1132 1229 2262 2873 4418 1132 Size: 97 1033 611 1545 2229 Fragments arranged by size: 2229 1545 1033 611 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5515 PciI A'CATG_T Cuts at: 4414 4414 Size: 5515 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2737 1108 Size: 902 727 3886 Fragments arranged by size: 3886 902 727 PmeI GTTT'AAAC Cuts at: 2819 2819 Size: 5515 PmlI CAC'GTG Cuts at: 2287 2287 Size: 5515 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2036 2068 3584 3616 5097 5129 2036 Size: 32 1516 32 1481 32 2422 Fragments arranged by size: 2422 1516 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2644 2644 Size: 5515 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5515 PsiI TTA'TAA Cuts at: 3085 3085 Size: 5515 PspOMI G'GGCC_C Cuts at: 2235 2235 Size: 5515 PspXI vC'TCGA_Gb Cuts at: 2673 2673 Size: 5515 PstI C_TGCA'G Cuts at: 830 2125 2244 2787 2867 830 Size: 1295 119 543 80 3478 Fragments arranged by size: 3478 1295 543 119 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5515 SacI G_AGCT'C Cuts at: 2622 2622 Size: 5515 SalI G'TCGA_C Cuts at: 1936 2857 1936 Size: 921 4594 SbfI CC_TGCA'GG Cuts at: 2867 2867 Size: 5515 ScaI AGT'ACT Cuts at: 2051 3763 5475 2051 Size: 1712 1712 2091 Fragments arranged by size: 2091 1712 1712 SfcI C'TryA_G Cuts at: 826 1045 2121 2240 2783 2863 3998 4679 4870 Size: 219 1076 119 543 80 1135 681 191 Cuts at: 4870 826 Size: 1471 Fragments arranged by size: 1471 1135 1076 681 543 219 191 119 80 SfoI GGC'GCC Cuts at: 2584 2584 Size: 5515 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5515 SmaI CCC'GGG Cuts at: 1872 2842 1872 Size: 970 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2673 3619 4520 4782 820 Size: 197 926 730 946 901 262 1553 Fragments arranged by size: 1553 946 926 901 730 262 197 SnaBI TAC'GTA Cuts at: 493 3306 493 Size: 2813 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5515 SphI G_CATG'C Cuts at: 1132 2873 1132 Size: 1741 3774 SspI AAT'ATT Cuts at: 5 52 3439 5 Size: 47 3387 2081 Fragments arranged by size: 3387 2081 47 StuI AGG'CCT Cuts at: 1517 2382 2655 1517 Size: 865 273 4377 Fragments arranged by size: 4377 865 273 StyI C'CwwG_G Cuts at: 513 1065 2004 2954 513 Size: 552 939 950 3074 Fragments arranged by size: 3074 950 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2812 3542 3559 3712 3897 4342 325 Size: 1255 1232 730 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1232 730 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2049 3761 5473 Size: 274 80 33 51 1443 72 1712 1712 Cuts at: 5473 96 Size: 138 Fragments arranged by size: 1712 1712 1443 274 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5475 5507 5475 Size: 32 5483 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5515 XbaI T'CTAG_A Cuts at: 2851 2851 Size: 5515 XhoI C'TCGA_G Cuts at: 1943 2673 1943 Size: 730 4785 XmaI C'CCGG_G Cuts at: 1870 2840 1870 Size: 970 4545 XmnI GAAnn'nnTTC Cuts at: 2561 3644 2561 Size: 1083 4432 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3323 276 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PfoI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI