Restriction map for pFN21AB7089
 (Circular) (Six-base) MAPSORT of: pFN21AB7089.seq  Check: 2962  from: 1  to: 5161



pFN21AB7089  5161 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:25  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2971     278 
   Size:         53      83     186     936    1435    2468

  Fragments arranged by size: 

                2468    1435     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2504    1937 
   Size:        567    4594
 
Acc65I G'GTAC_C

Cuts at:   2482    2482 
   Size:       5161
 
AclI AA'CG_TT

Cuts at:   1563    3288    3661    1563 
   Size:       1725     373    3063

  Fragments arranged by size: 

                3063    1725     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3224    4608    4876    1632 
   Size:       1592    1384     268    1917

  Fragments arranged by size: 

                1917    1592    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4964
 
AflIII A'CryG_T

Cuts at:   1203    2205    4060    5037    1203 
   Size:       1002    1855     977    1327

  Fragments arranged by size: 

                1855    1327    1002     977
 
AgeI A'CCGG_T

Cuts at:   3992    3992 
   Size:       5161
 
AhdI GACnn_n'nnGTC

Cuts at:   3890    3890 
   Size:       5161
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5161
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5161
 
AlwNI CAG_nnn'CTG

Cuts at:   2904    4476    2904 
   Size:       1572    3589
 
ApaLI G'TGCA_C

Cuts at:   3218    4374    3218 
   Size:       1156    4005
 
ApoI r'AATT_y

Cuts at:   1493    2470    2696    2848    4957    1493 
   Size:        977     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     977     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3715     160 
   Size:        872    2683    1606

  Fragments arranged by size: 

                2683    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5161
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2486    1870 
   Size:         73     543    4545

  Fragments arranged by size: 

                4545     543      73
 
BamHI G'GATC_C

Cuts at:   2491    2858    2491 
   Size:        367    4794
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2482    3937     618 
   Size:        325     217     627     695    1455    1842

  Fragments arranged by size: 

                1842    1455     695     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2415    2424    2480    1391 
   Size:       1024       9      56    4072

  Fragments arranged by size: 

                4072    1024      56       9
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4190
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2315    2349    3352    3386    1903 
   Size:         34     378      34    1003      34    3678

  Fragments arranged by size: 

                3678    1003     378      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3053    4263    3053 
   Size:       1210    3951
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5161
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2428    3772     136 
   Size:        107     122      71     631     605     756    1344    1525

  Fragments arranged by size: 

                1525    1344     756     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   5156    5156 
   Size:       5161
 
BlpI GC'TnA_GC

Cuts at:   2578    2578 
   Size:       5161
 
Bme1580I G_kGCm'C

Cuts at:   1790    3222    4378    1790 
   Size:       1432    1156    2573

  Fragments arranged by size: 

                2573    1432    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5161
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2892    3850     442 
   Size:        416      53      83     399      24    1475     958    1753

  Fragments arranged by size: 

                1753    1475     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5161
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2410    3821    1239 
   Size:       1171    1411    2579

  Fragments arranged by size: 

                2579    1411    1171
 
Bpu10I CC'TnA_GC

Cuts at:   2111    2111 
   Size:       5161
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2156    3286    4151    4449    2156 
   Size:       1130     865     298    2868

  Fragments arranged by size: 

                2868    1130     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3824     882 
   Size:        617    2325    2219

  Fragments arranged by size: 

                2325    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2206    2952     493 
   Size:        711    1002     746    2702

  Fragments arranged by size: 

                2702    1002     746     711
 
BsaBI GATnn'nnATC

Cuts at:   5155    5155 
   Size:       5161
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2379    2968    3350    3985 
   Size:         53      83     186     936     846     589     382     635

Cuts at:   3985     275 
   Size:       1451

  Fragments arranged by size: 

                1451     936     846     635     589     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3594    3992    4266    4413    4824    3594 
   Size:        398     274     147     411    3931

  Fragments arranged by size: 

                3931     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4936

  Fragments arranged by size: 

                4936     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5161
 
BseYI C'CCAG_C

Cuts at:   4364    4860    4364 
   Size:        496    4665
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4588
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2107    2397    2611    3372    4400    1540 
   Size:        275     186     106     290     214     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     290     275     214     186     106
            
 
BsiHKAI G_wGCw'C

Cuts at:   2097    3222    3307    4378    2097 
   Size:       1125      85    1071    2880

  Fragments arranged by size: 

                2880    1125    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2672    2765    2672 
   Size:         93    5068
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2097    2415    2424    2480    3222    3307    4378 
   Size:        399     307     318       9      56     742      85    1071

Cuts at:   4378    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     399     318     307      85      56
                   9
 
BspEI T'CCGG_A

Cuts at:   4824    4824 
   Size:       5161
 
BspHI T'CATG_A

Cuts at:   3050    4780    3050 
   Size:       1730    3431
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2516     844 
   Size:       1672    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3048    1394 
   Size:        429    1225    3507

  Fragments arranged by size: 

                3507    1225     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3656    3830      65 
   Size:       1183    2408     174    1396

  Fragments arranged by size: 

                2408    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3805    3992    1700 
   Size:        110    1995     187    2869

  Fragments arranged by size: 

                2869    1995     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5161
 
BssHII G'CGCG_C

Cuts at:   1925    2136    2138    1925 
   Size:        211       2    4948

  Fragments arranged by size: 

                4948     211       2
 
BssSI C'ACGA_G

Cuts at:   3221    4233    3221 
   Size:       1012    4149
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5161
 
BstBI TT'CG_AA

Cuts at:   3965    3965 
   Size:       5161
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5161
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2290    2290 
   Size:       5161
 
BstYI r'GATC_y

Cuts at:   1972    2491    2858    3243    3260    4712    4798    4810    5156 
   Size:        519     367     385      17    1452      86      12     346

Cuts at:   5156    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     519     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2382    2391     513 
   Size:        552    1317       9    3283

  Fragments arranged by size: 

                3283    1317     552       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2269     487 
   Size:        683      56    1043    3379

  Fragments arranged by size: 

                3379    1043     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2677    3471    3491    2677 
   Size:        794      20    4347

  Fragments arranged by size: 

                4347     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2301    1554 
   Size:        747    4414
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5126
 
DraI TTT'AAA

Cuts at:   2465    2465 
   Size:       5161
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4168     809 
   Size:        507    2852    1802

  Fragments arranged by size: 

                2852    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2280    2394    2608 
   Size:         54    1425      50     275      18     450     114     214

Cuts at:   2608    3497    5126       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     450     275     214     114      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2394    2608    1537 
   Size:        275     582     214    4090

  Fragments arranged by size: 

                4090     582     275     214
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2069    3091    1353 
   Size:        716    1022    3423

  Fragments arranged by size: 

                3423    1022     716
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3748    4122    4268     387 
   Size:       1064     138    2159     374     146    1280

  Fragments arranged by size: 

                2159    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2410    3224    3821    4608    4876    1239 
   Size:        393     778     814     597     787     268    1524

  Fragments arranged by size: 

                1524     814     787     778     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2470    2848    2470 
   Size:        378    4783
 
FspI TGC'GCA

Cuts at:   3667    3667 
   Size:       5161
 
HaeII r_GCGC'y

Cuts at:   1678    2297    4308    1678 
   Size:        619    2011    2531

  Fragments arranged by size: 

                2531    2011     619
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3802    3834    3876    3908    1373 
   Size:         32     135      32    2230      32      42      32    2626

  Fragments arranged by size: 

                2626    2230     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2505    2751     669 
   Size:        988     281     567     246    3079

  Fragments arranged by size: 

                3079     988     567     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5161
 
HpaI GTT'AAC

Cuts at:   2751    2751 
   Size:       5161
 
KpnI G_GTAC'C

Cuts at:   2486    2486 
   Size:       5161
 
MfeI C'AATT_G

Cuts at:   2760    2760 
   Size:       5161
 
MluI A'CGCG_T

Cuts at:   5037    5037 
   Size:       5161
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2022    2127    4275    4459    1305 
   Size:        717     105    2148     184    2007

  Fragments arranged by size: 

                2148    2007     717     184     105
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3339

  Fragments arranged by size: 

                3339    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3119    3478    3637     518 
   Size:        648    1953     359     159    2042

  Fragments arranged by size: 

                2042    1953     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2253    2393    2577    3254    4402    4647    1606 
   Size:        647     140     184     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     647     245     184     140
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4609
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5161
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5161
 
NotI GC'GGCC_GC

Cuts at:   2394    2608    2394 
   Size:        214    4947
 
NspI r_CATG'y

Cuts at:   1132    1229    2519    4064    1132 
   Size:         97    1290    1545    2229

  Fragments arranged by size: 

                2229    1545    1290      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5161
 
PciI A'CATG_T

Cuts at:   4060    4060 
   Size:       5161
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5161
 
PmeI GTTT'AAAC

Cuts at:   2465    2465 
   Size:       5161
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3230    3262    4743    4775    3230 
   Size:         32    1481      32    3616

  Fragments arranged by size: 

                3616    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5161
 
PsiI TTA'TAA

Cuts at:   2731    2731 
   Size:       5161
 
PstI C_TGCA'G

Cuts at:    830    2328    2513     830 
   Size:       1498     185    3478

  Fragments arranged by size: 

                3478    1498     185
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5161
 
SacII CC_GC'GG

Cuts at:   2394    2394 
   Size:       5161
 
SalI G'TCGA_C

Cuts at:   1936    2503    1936 
   Size:        567    4594
 
SbfI CC_TGCA'GG

Cuts at:   2328    2513    2328 
   Size:        185    4976
 
ScaI AGT'ACT

Cuts at:   3409    5121    3409 
   Size:       1712    3449
 
SfcI C'TryA_G

Cuts at:    826    1045    2324    2509    3644    4325    4516     826 
   Size:        219    1279     185    1135     681     191    1471

  Fragments arranged by size: 

                1471    1279    1135     681     219     191     185
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5161
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5161
 
SmaI CCC'GGG

Cuts at:   1872    2488    1872 
   Size:        616    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2171    3265    4166    4428     820 
   Size:        197     926     228    1094     901     262    1553

  Fragments arranged by size: 

                1553    1094     926     901     262     228     197
 
SnaBI TAC'GTA

Cuts at:    493    2952     493 
   Size:       2459    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5161
 
SphI G_CATG'C

Cuts at:   1132    2519    1132 
   Size:       1387    3774
 
SspI AAT'ATT

Cuts at:      5      52    3085       5 
   Size:         47    3033    2081

  Fragments arranged by size: 

                3033    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5161
 
StyI C'CwwG_G

Cuts at:    513    1065    2145    2600     513 
   Size:        552    1080     455    3074

  Fragments arranged by size: 

                3074    1080     552     455
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3188    3205    3358    3543    3988     325 
   Size:       1255    1608      17     153     185     445    1498

  Fragments arranged by size: 

                1608    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2208    3407    5119 
   Size:        274      80      33      51    1443     231    1199    1712

Cuts at:   5119      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1199     274     231     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5121    5153    5121 
   Size:         32    5129
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5161
 
XbaI T'CTAG_A

Cuts at:   2497    2497 
   Size:       5161
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2173    2173 
   Size:       5161
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5161
 
XmaI C'CCGG_G

Cuts at:   1870    2486    1870 
   Size:        616    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3290    3290 
   Size:       5161
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2969     276 
   Size:         53      83     186     936    1435    2468

  Fragments arranged by size: 

                2468    1435     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI     PstI     PvuI    SacII     SalI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI
    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SanDI
     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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