Restriction map for
pFN21AB7083
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI BspDI BspEI BspLU11I
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DrdI DsaI Eam1105I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HindIII HinfI HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MroI MseI MslI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 189 GAATTC [GA]^AATT[CT]
Aor51HI 257 AGCGCT AGC^GCT
ApoI 189 GAATTC [GA]^AATT[CT]
Asp700I 189 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 144 GGTCC G^G[AT]CC
BalI 320 TGGCCA TGG^CCA
BanI 343 GGCACC G^G[CT][GA]CC
BanII 39 GGGCTC G[GA]GC[CT]^C
BcnI 310 CCGGG CC^[GC]GG
BpmI 273 CTCCAG CTGGAG(16/14)
BsiEI 290 CGGCCG CG[GA][CT]^CG
Bsp1407I 125 TGTACA T^GTACA
BspHI 185 TCATGA T^CATGA
BspMI 141 GCAGGT ACCTGC(4/8)
BsrDI 175 GCAATG GCAATG(2/0)
BsrFI 157 ACCGGC [GA]^CCGG[CT]
BsrGI 125 TGTACA T^GTACA
BsrI 368 CCAGT ACTGG(1/-1)
BstF5I 148 CATCC GGATG(2/0)
BstXI 170 CCAAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 281 AGATCC [GA]^GATC[CT]
Cfr10I 157 ACCGGC [GA]^CCGG[CT]
Cfr13I 144 GGTCC G^G[ACGT]CC
DraIII 328 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 290 CGGCCG C^GGCCG
EarI 165 CTCTTC CTCTTC(1/4)
EclXI 290 CGGCCG C^GGCCG
Eco47III 257 AGCGCT AGC^GCT
Eco52I 290 CGGCCG C^GGCCG
EcoRI 189 GAATTC G^AATTC
FauI 236 CCCGC CCCGC(4/6)
FokI 148 CATCC GGATG(9/13)
HaeII 257 AGCGCT [GA]GCGC^[CT]
HincII 199 GTCAAC GT[CT]^[GA]AC
HindII 199 GTCAAC GT[CT]^[GA]AC
Ksp632I 165 CTCTTC CTCTTC(1/4)
MflI 281 AGATCC [GA]^GATC[CT]
MluNI 320 TGGCCA TGG^CCA
MscI 320 TGGCCA TGG^CCA
NciI 310 CCGGG CC^[GC]GG
NlaIII 186 CATG CATG^
RcaI 185 TCATGA T^CATGA
Sau96I 144 GGTCC G^G[ACGT]CC
SspBI 125 TGTACA T^GTACA
Tsp509I 190 AATT ^AATT
TspRI 116 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 281 AGATCC [GA]^GATC[CT]
XmnI 189 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 126 GTAC GT^AC
363 GTAC
AluI 109 AGCT AG^CT
317 AGCT
AlwI 282 GATCC GGATC(4/5)
182 GGATC
BbvI 95 GCAGC GCAGC(8/12)
303 GCTGC
BmyI 39 GGGCTC G[AGT]GC[ACT]^C
342 GGGCAC
Bsp1286I 39 GGGCTC G[AGT]GC[ACT]^C
342 GGGCAC
BstNI 240 CCTGG CC^[AT]GG
275 CCAGG
DdeI 59 CTAAG C^T[ACGT]AG
65 CTCAG
DpnII 183 GATC ^GATC
282 GATC
EaeI 290 CGGCCG [CT]^GGCC[GA]
320 TGGCCA
EcoRII 240 CCTGG ^CC[AT]GG
275 CCAGG
EcoT14I 170 CCAAGG C^C[AT][AT]GG
347 CCAAGG
HapII 158 CCGG C^CGG
310 CCGG
HpaII 158 CCGG C^CGG
310 CCGG
MaeIII 55 GTGAC ^GT[ACGT]AC
355 GTCAC
MboI 183 GATC ^GATC
282 GATC
MspI 158 CCGG C^CGG
310 CCGG
MvaI 240 CCTGG CC^[AT]GG
275 CCAGG
NdeII 183 GATC ^GATC
282 GATC
NlaIV 40 GGCTCC GG[ACGT]^[ACGT]CC
343 GGCACC
RsaI 126 GTAC GT^AC
363 GTAC
Sau3AI 183 GATC ^GATC
282 GATC
SfaNI 161 GCATC GCATC(5/9)
218 GCATC
StyI 170 CCAAGG C^C[AT][AT]GG
347 CCAAGG
TaqI 212 TCGA T^CGA
262 TCGA
TseI 95 GCAGC G^C[AT]GC
303 GCTGC
Tsp45I 55 GTGAC ^GT[GC]AC
355 GTCAC
TthHB8I 212 TCGA T^CGA
262 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI BsaJI BsiYI BslI BssKI
Cac8I CfoI Fnu4HI HaeIII HhaI
HinP1I HphI ItaI MboII MnlI
MwoI ScrFI