(Circular) (Six-base) MAPSORT of: pFN21AB7035.seq Check: 1693 from: 1 to: 5983 pFN21AB7035 5983 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2637 3326 1937 Size: 700 689 4594 Fragments arranged by size: 4594 700 689 Acc65I G'GTAC_C Cuts at: 2236 3304 2236 Size: 1068 4915 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2925 4046 5430 5698 1632 Size: 1293 1121 1384 268 1917 Fragments arranged by size: 1917 1384 1293 1121 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5786 AflIII A'CryG_T Cuts at: 1203 4882 5859 1203 Size: 3679 977 1327 Fragments arranged by size: 3679 1327 977 AgeI A'CCGG_T Cuts at: 4814 4814 Size: 5983 AhdI GACnn_n'nnGTC Cuts at: 2110 2222 4712 2110 Size: 112 2490 3381 Fragments arranged by size: 3381 2490 112 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5983 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5983 AlwNI CAG_nnn'CTG Cuts at: 3726 5298 3726 Size: 1572 4411 ApaLI G'TGCA_C Cuts at: 4040 5196 4040 Size: 1156 4827 ApoI r'AATT_y Cuts at: 1493 3292 3518 3670 5779 1493 Size: 1799 226 152 2109 1697 Fragments arranged by size: 2109 1799 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 3067 3308 1870 Size: 73 1124 241 4545 Fragments arranged by size: 4545 1124 241 73 BamHI G'GATC_C Cuts at: 2078 3313 3680 2078 Size: 1235 367 4381 Fragments arranged by size: 4381 1235 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2236 2756 3304 4759 618 Size: 325 217 627 449 520 548 1455 1842 Fragments arranged by size: 1842 1455 627 548 520 449 325 217 BanII G_rGCy'C Cuts at: 1391 2714 3302 1391 Size: 1323 588 4072 Fragments arranged by size: 4072 1323 588 BbeI G_GCGC'C Cuts at: 2760 2760 Size: 5983 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2411 2656 2913 928 Size: 971 512 245 257 3998 Fragments arranged by size: 3998 971 512 257 245 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4174 4208 1903 Size: 34 50 34 2153 34 3678 Fragments arranged by size: 3678 2153 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3875 5085 3875 Size: 1210 4773 BclI T'GATC_A Cuts at: 1548 1548 Size: 5983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4594 136 Size: 107 122 71 631 605 2922 1525 Fragments arranged by size: 2922 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5978 5978 Size: 5983 BlpI GC'TnA_GC Cuts at: 3400 3400 Size: 5983 Bme1580I G_kGCm'C Cuts at: 1790 2091 3068 3183 4044 5200 1790 Size: 301 977 115 861 1156 2573 Fragments arranged by size: 2573 1156 977 861 301 115 BmgBI CAC'GTC Cuts at: 1318 3087 1318 Size: 1769 4214 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3714 4672 442 Size: 416 53 83 399 24 2297 958 1753 Fragments arranged by size: 2297 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2380 2380 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2659 4643 1239 Size: 1420 1984 2579 Fragments arranged by size: 2579 1984 1420 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4108 4973 5271 4108 Size: 865 298 4820 Fragments arranged by size: 4820 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2209 3187 4646 882 Size: 617 710 978 1459 2219 Fragments arranged by size: 2219 1459 978 710 617 BsaAI yAC'GTr Cuts at: 493 1204 3774 493 Size: 711 2570 2702 Fragments arranged by size: 2702 2570 711 BsaBI GATnn'nnATC Cuts at: 5977 5977 Size: 5983 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2757 2926 3790 4172 Size: 53 83 186 936 1224 169 864 382 Cuts at: 4172 4807 275 Size: 635 1451 Fragments arranged by size: 1451 1224 936 864 635 382 186 169 83 53 BsaWI w'CCGG_w Cuts at: 4416 4814 5088 5235 5646 4416 Size: 398 274 147 411 4753 Fragments arranged by size: 4753 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2285 2315 3260 3290 1178 Size: 30 165 30 882 30 945 30 3871 Fragments arranged by size: 3871 945 882 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2074 2620 2813 3281 1186 Size: 888 546 193 468 3888 Fragments arranged by size: 3888 888 546 468 193 BseYI C'CCAG_C Cuts at: 2229 5186 5682 2229 Size: 2957 496 2530 Fragments arranged by size: 2957 2530 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 2396 2481 1163 Size: 573 450 210 85 4665 Fragments arranged by size: 4665 573 450 210 85 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3433 4194 5222 1540 Size: 275 186 1432 761 1028 2301 Fragments arranged by size: 2301 1432 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2654 2714 3281 4044 4129 5200 2654 Size: 60 567 763 85 1071 3437 Fragments arranged by size: 3437 1071 763 567 85 60 BsmI GAATG_Cn' Cuts at: 3494 3587 3494 Size: 93 5890 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2654 2714 3068 3183 3281 3302 Size: 399 301 563 60 354 115 98 21 Cuts at: 3302 4044 4129 5200 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 563 399 354 301 115 98 85 60 21 BspEI T'CCGG_A Cuts at: 5646 5646 Size: 5983 BspHI T'CATG_A Cuts at: 3872 5602 3872 Size: 1730 4253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3338 844 Size: 2494 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3870 1394 Size: 429 2047 3507 Fragments arranged by size: 3507 2047 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3021 4478 4652 65 Size: 1183 1773 1457 174 1396 Fragments arranged by size: 1773 1457 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2233 2922 4627 4814 1700 Size: 110 423 689 1705 187 2869 Fragments arranged by size: 2869 1705 689 423 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5983 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5983 BssSI C'ACGA_G Cuts at: 4043 5055 4043 Size: 1012 4971 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5983 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5983 BstXI CCAn_nnnn'nTGG Cuts at: 2753 2753 Size: 5983 BstYI r'GATC_y Cuts at: 1972 2078 3313 3680 4065 4082 5534 5620 5632 Size: 106 1235 367 385 17 1452 86 12 Cuts at: 5632 5978 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1235 385 367 346 106 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5431 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2903 487 Size: 683 56 1677 3567 Fragments arranged by size: 3567 1677 683 56 BtsI GCAGTG_nn' Cuts at: 2443 2747 3499 4293 4313 2443 Size: 304 752 794 20 4113 Fragments arranged by size: 4113 794 752 304 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5948 DraI TTT'AAA Cuts at: 2193 3287 2193 Size: 1094 4889 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4990 809 Size: 507 3674 1802 Fragments arranged by size: 3674 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 3430 4319 Size: 54 1425 50 275 18 294 1306 889 Cuts at: 4319 5948 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1306 889 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3430 1537 Size: 275 1618 4090 Fragments arranged by size: 4090 1618 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2207 2480 2534 2568 2642 2790 3913 1353 Size: 854 273 54 34 74 148 1123 3423 Fragments arranged by size: 3423 1123 854 273 148 74 54 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2513 2838 4570 4944 5090 387 Size: 1064 138 924 325 1732 374 146 1280 Fragments arranged by size: 1732 1280 1064 924 374 325 146 138 EcoICRI GAG'CTC Cuts at: 2712 2712 Size: 5983 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2659 2925 4046 4643 5430 5698 1239 Size: 393 1027 266 1121 597 787 268 1524 Fragments arranged by size: 1524 1121 1027 787 597 393 268 266 EcoNI CCTnn'n_nnAGG Cuts at: 2135 2135 Size: 5983 EcoO109I rG'GnC_Cy Cuts at: 3093 3093 Size: 5983 EcoRI G'AATT_C Cuts at: 3292 3670 3292 Size: 378 5605 FspI TGC'GCA Cuts at: 4489 4489 Size: 5983 HaeII r_GCGC'y Cuts at: 1678 2252 2760 5130 1678 Size: 574 508 2370 2531 Fragments arranged by size: 2531 2370 574 508 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2096 2128 2208 2240 2865 Size: 32 135 32 524 32 80 32 625 Cuts at: 2865 2897 3010 3042 4624 4656 4698 4730 1373 Size: 32 113 32 1582 32 42 32 2626 Fragments arranged by size: 2626 1582 625 524 135 113 80 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2959 3327 3573 669 Size: 988 281 1021 368 246 3079 Fragments arranged by size: 3079 1021 988 368 281 246 HindIII A'AGCT_T Cuts at: 748 2270 748 Size: 1522 4461 HpaI GTT'AAC Cuts at: 3573 3573 Size: 5983 KasI G'GCGC_C Cuts at: 2756 2756 Size: 5983 KpnI G_GTAC'C Cuts at: 2240 3308 2240 Size: 1068 4915 MfeI C'AATT_G Cuts at: 3582 3582 Size: 5983 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5097 5281 1305 Size: 3792 184 2007 Fragments arranged by size: 3792 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4161 Fragments arranged by size: 4161 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3203 3260 3941 4300 4459 518 Size: 648 2037 57 681 359 159 2042 Fragments arranged by size: 2042 2037 681 648 359 159 57 MspA1I CmG'CkG Cuts at: 1606 2689 3399 4076 5224 5469 1606 Size: 1083 710 677 1148 245 2120 Fragments arranged by size: 2120 1148 1083 710 677 245 NaeI GCC'GGC Cuts at: 2924 2924 Size: 5983 NarI GG'CG_CC Cuts at: 2757 2757 Size: 5983 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5431 NdeI CA'TA_TG Cuts at: 387 387 Size: 5983 NgoMIV G'CCGG_C Cuts at: 2922 2922 Size: 5983 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 2200 2663 3139 3341 4886 1132 Size: 97 971 463 476 202 1545 2229 Fragments arranged by size: 2229 1545 971 476 463 202 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5983 PciI A'CATG_T Cuts at: 4882 4882 Size: 5983 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5983 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2604 2636 4052 4084 5565 5597 2604 Size: 32 1416 32 1481 32 2990 Fragments arranged by size: 2990 1481 1416 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3093 3093 Size: 5983 PshAI GACnn'nnGTC Cuts at: 1613 2513 3199 1613 Size: 900 686 4397 Fragments arranged by size: 4397 900 686 PsiI TTA'TAA Cuts at: 3553 3553 Size: 5983 PstI C_TGCA'G Cuts at: 830 2892 3335 830 Size: 2062 443 3478 Fragments arranged by size: 3478 2062 443 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5983 SacI G_AGCT'C Cuts at: 2714 2714 Size: 5983 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SapI GCTCTTCn'nnn_ Cuts at: 2480 2642 2480 Size: 162 5821 SbfI CC_TGCA'GG Cuts at: 3335 3335 Size: 5983 ScaI AGT'ACT Cuts at: 4231 5943 4231 Size: 1712 4271 SfcI C'TryA_G Cuts at: 826 1045 2888 3331 4466 5147 5338 826 Size: 219 1843 443 1135 681 191 1471 Fragments arranged by size: 1843 1471 1135 681 443 219 191 SfoI GGC'GCC Cuts at: 2758 2758 Size: 5983 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 2922 1700 Size: 1222 4761 SmaI CCC'GGG Cuts at: 1872 3310 1872 Size: 1438 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4087 4988 5250 820 Size: 197 926 2144 901 262 1553 Fragments arranged by size: 2144 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3774 493 Size: 3281 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5983 SphI G_CATG'C Cuts at: 1132 3139 3341 1132 Size: 2007 202 3774 Fragments arranged by size: 3774 2007 202 SspI AAT'ATT Cuts at: 5 52 3907 5 Size: 47 3855 2081 Fragments arranged by size: 3855 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5983 StyI C'CwwG_G Cuts at: 513 1065 2241 2427 2490 3422 513 Size: 552 1176 186 63 932 3074 Fragments arranged by size: 3074 1176 932 552 186 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2243 3264 4010 4027 4180 4365 4810 Size: 1255 663 1021 746 17 153 185 445 Cuts at: 4810 325 Size: 1498 Fragments arranged by size: 1498 1255 1021 746 663 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3210 4229 5941 Size: 274 80 33 51 1443 1233 1019 1712 Cuts at: 5941 96 Size: 138 Fragments arranged by size: 1712 1443 1233 1019 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2719 2751 3090 3122 3260 3292 5943 5975 2719 Size: 32 339 32 138 32 2651 32 2727 Fragments arranged by size: 2727 2651 339 138 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5983 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5983 XmaI C'CCGG_G Cuts at: 1870 3308 1870 Size: 1438 4545 XmnI GAAnn'nnTTC Cuts at: 2978 4112 2978 Size: 1134 4849 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI