Restriction map for
pFN21AB5680
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BbeI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BsaI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DrdI
DsaI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HpaI KasI KpnI KspI MaeI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 1192 GACGCC G[GA]^CG[CT]C
AflIII 1244 ACGTGT A^C[GA][CT]GT
AhdI 355 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 386 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 277 CCTGAGG CC^T[ACGT]AGG
ApaI 746 GGGCCC GGGCC^C
AspEI 355 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1075 GAGCTC G[AT]GC[AT]^C
BbrPI 1243 CACGTG CAC^GTG
BclI 1337 TGATCA T^GATCA
BlpI 1260 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1260 GCTCAGC GC^T[ACGT]AGC
BsaAI 1243 CACGTG [CT]AC^GT[GA]
BsaBI 1338 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1192 GACGCC G[GA]^CG[CT]C
BsaWI 359 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1075 GAGCTC G[AT]GC[AT]^C
BspMI 788 ACCTGC ACCTGC(4/8)
BsrFI 1225 GCCGGT [GA]^CCGG[CT]
Bsu36I 277 CCTGAGG CC^T[ACGT]AGG
CelII 1260 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1225 GCCGGT [GA]^CCGG[CT]
Eam1105I 355 GACTTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 394 GAAGAG CTCTTC(1/4)
Eco81I 277 CCTGAGG CC^T[ACGT]AGG
FbaI 1337 TGATCA T^GATCA
HaeII 760 GGCGCT [GA]GCGC^[CT]
Hin1I 1192 GACGCC G[GA]^CG[CT]C
HindIII 1066 AAGCTT A^AGCTT
HinfI 273 GACTC G^A[ACGT]TC
Ksp632I 394 GAAGAG CTCTTC(1/4)
MamI 1338 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 863 TTAA T^TAA
MslI 1080 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 120 GCGGCCGC GC^GGCCGC
PflMI 309 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 273 GACTC GAGTC(4/5)
PmaCI 1243 CACGTG CAC^GTG
PmlI 1243 CACGTG CAC^GTG
PshAI 240 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 181 CAGCTG CAG^CTG
SacI 1075 GAGCTC GAGCT^C
ScaI 641 AGTACT AGT^ACT
SexAI 1303 ACCAGGT A^CC[AT]GGT
SfaNI 1212 GCATC GCATC(5/9)
SmaI 719 CCCGGG CCC^GGG
TaqI 251 TCGA T^CGA
Tru9I 863 TTAA T^TAA
TthHB8I 251 TCGA T^CGA
Van91I 309 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 719 CCCGGG C^CCGGG
XmaI 719 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BalI 413 TGGCCA TGG^CCA
560 TGGCCA
BbsI 189 GAAGAC GAAGAC(2/6)
918 GAAGAC
BpuAI 189 GAAGAC GAAGAC(2/6)
918 GAAGAC
BsmAI 271 GAGAC GTCTC(1/5)
1294 GAGAC
BstYI 536 AGATCC [GA]^GATC[CT]
1094 AGATCC
DraIII 371 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
427 CACCGCGTG
EagI 121 CGGCCG C^GGCCG
978 CGGCCG
EclXI 121 CGGCCG C^GGCCG
978 CGGCCG
Eco52I 121 CGGCCG C^GGCCG
978 CGGCCG
EcoT14I 563 CCAAGG C^C[AT][AT]GG
1196 CCAAGG
HgaI 1192 GACGC GACGC(5/10)
599 GCGTC
MflI 536 AGATCC [GA]^GATC[CT]
1094 AGATCC
MluNI 413 TGGCCA TGG^CCA
560 TGGCCA
MscI 413 TGGCCA TGG^CCA
560 TGGCCA
PpuMI 818 GGGACCT [GA]G^G[AT]CC[CT]
1157 AGGACCC
PstI 232 CTGCAG CTGCA^G
604 CTGCAG
SfcI 232 CTGCAG C^T[GA][CT]AG
604 CTGCAG
StyI 563 CCAAGG C^C[AT][AT]GG
1196 CCAAGG
XcmI 305 CCATCCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
563 CCAAGGACGAGGTGG
XhoII 536 AGATCC [GA]^GATC[CT]
1094 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SmlI TseI Tsp45I TspRI