(Circular) (Six-base) MAPSORT of: pFN21AB5680.seq Check: 3119 from: 1 to: 6145 pFN21AB5680 6145 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3955 278 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3488 1937 Size: 1551 4594 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2418 2483 2663 4208 5592 5860 1632 Size: 786 65 180 1545 1384 268 1917 Fragments arranged by size: 1917 1545 1384 786 268 180 65 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 3249 5044 6021 1203 Size: 2046 1795 977 1327 Fragments arranged by size: 2046 1795 1327 977 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 2365 4874 2365 Size: 2509 3636 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6145 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6145 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2250 2282 2720 2752 2250 Size: 32 438 32 5643 Fragments arranged by size: 5643 438 32 32 AlwNI CAG_nnn'CTG Cuts at: 2396 3888 5460 2396 Size: 1492 1572 3081 Fragments arranged by size: 3081 1572 1492 ApaI G_GGCC'C Cuts at: 2755 2755 Size: 6145 ApaLI G'TGCA_C Cuts at: 4202 5358 4202 Size: 1156 4989 ApoI r'AATT_y Cuts at: 1493 3454 3680 3832 5941 1493 Size: 1961 226 152 2109 1697 Fragments arranged by size: 2109 1961 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2724 2957 3125 3470 1870 Size: 73 781 233 168 345 4545 Fragments arranged by size: 4545 781 345 233 168 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2930 2963 2930 Size: 33 6112 BamHI G'GATC_C Cuts at: 3475 3842 3475 Size: 367 5778 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2102 2622 2912 3466 4921 Size: 325 217 627 315 520 290 554 1455 Cuts at: 4921 618 Size: 1842 Fragments arranged by size: 1842 1455 627 554 520 325 315 290 217 BanII G_rGCy'C Cuts at: 1391 2375 2755 3084 3191 3464 1391 Size: 984 380 329 107 273 4072 Fragments arranged by size: 4072 984 380 329 273 107 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2201 2930 928 Size: 971 302 729 4143 Fragments arranged by size: 4143 971 729 302 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4336 4370 1903 Size: 34 2399 34 3678 Fragments arranged by size: 3678 2399 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5247 4037 Size: 1210 4935 BclI T'GATC_A Cuts at: 1548 3342 1548 Size: 1794 4351 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4756 136 Size: 107 122 71 631 605 3084 1525 Fragments arranged by size: 3084 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6140 6140 Size: 6145 BlpI GC'TnA_GC Cuts at: 3266 3562 3266 Size: 296 5849 Bme1580I G_kGCm'C Cuts at: 1790 2483 2755 2865 4206 5362 1790 Size: 693 272 110 1341 1156 2573 Fragments arranged by size: 2573 1341 1156 693 272 110 BmgBI CAC'GTC Cuts at: 1318 2014 3294 1318 Size: 696 1280 4169 Fragments arranged by size: 4169 1280 696 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2373 3409 3876 Size: 416 53 83 399 24 956 1036 467 Cuts at: 3876 4834 442 Size: 958 1753 Fragments arranged by size: 1753 1036 958 956 467 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2602 2855 2987 4805 1239 Size: 1363 253 132 1818 2579 Fragments arranged by size: 2579 1818 1363 253 132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2075 2318 2531 2813 3140 4270 5135 5433 2075 Size: 243 213 282 327 1130 865 298 2787 Fragments arranged by size: 2787 1130 865 327 298 282 243 213 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4808 882 Size: 617 3309 2219 Fragments arranged by size: 3309 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3250 3936 493 Size: 711 2046 686 2702 Fragments arranged by size: 2702 2046 711 686 BsaBI GATnn'nnATC Cuts at: 3347 6139 3347 Size: 2792 3353 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3198 3952 4334 4969 Size: 53 83 186 936 1665 754 382 635 Cuts at: 4969 275 Size: 1451 Fragments arranged by size: 1665 1451 936 754 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2364 4578 4976 5250 5397 5808 2364 Size: 2214 398 274 147 411 2701 Fragments arranged by size: 2701 2214 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2720 2750 1178 Size: 30 165 30 1317 30 4573 Fragments arranged by size: 4573 1317 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2300 2309 2360 2363 2366 3110 3338 Size: 892 222 9 51 3 3 744 228 Cuts at: 3338 1186 Size: 3993 Fragments arranged by size: 3993 892 744 228 222 51 9 3 3 BseYI C'CCAG_C Cuts at: 2470 3007 5348 5844 2470 Size: 537 2341 496 2771 Fragments arranged by size: 2771 2341 537 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2024 2780 3009 1163 Size: 573 288 756 229 4299 Fragments arranged by size: 4299 756 573 288 229 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2129 2369 2668 2986 3040 3444 Size: 275 186 128 240 299 318 54 404 Cuts at: 3444 3595 4356 5384 1540 Size: 151 761 1028 2301 Fragments arranged by size: 2301 1028 761 404 318 299 275 240 186 151 128 54 BsiHKAI G_wGCw'C Cuts at: 3084 4206 4291 5362 3084 Size: 1122 85 1071 3867 Fragments arranged by size: 3867 1122 1071 85 BsmI GAATG_Cn' Cuts at: 3656 3749 3656 Size: 93 6052 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2375 2483 2755 2865 3084 3191 3464 Size: 399 585 108 272 110 219 107 273 Cuts at: 3464 4206 4291 5362 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 585 399 273 272 219 110 108 107 85 BspEI T'CCGG_A Cuts at: 5808 5808 Size: 6145 BspHI T'CATG_A Cuts at: 4034 5764 4034 Size: 1730 4415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2802 3500 844 Size: 1958 698 3489 Fragments arranged by size: 3489 1958 698 BsrBI CCG'CTC Cuts at: 1394 1823 2125 2203 2290 4032 1394 Size: 429 302 78 87 1742 3507 Fragments arranged by size: 3507 1742 429 302 87 78 BsrDI GCAATG_nn' Cuts at: 65 1248 4640 4814 65 Size: 1183 3392 174 1396 Fragments arranged by size: 3392 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3230 4789 4976 1700 Size: 110 1420 1559 187 2869 Fragments arranged by size: 2869 1559 1420 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6145 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6145 BssSI C'ACGA_G Cuts at: 4205 5217 4205 Size: 1012 5133 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6145 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6145 BstYI r'GATC_y Cuts at: 1972 2541 3099 3475 3842 4227 4244 5696 5782 Size: 569 558 376 367 385 17 1452 86 Cuts at: 5782 5794 6140 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 569 558 385 376 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2283 2283 Size: 6145 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5593 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3203 487 Size: 683 56 1977 3429 Fragments arranged by size: 3429 1977 683 56 BtsI GCAGTG_nn' Cuts at: 2186 2246 2997 3661 4455 4475 2186 Size: 60 751 664 794 20 3856 Fragments arranged by size: 3856 794 751 664 60 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6110 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DraIII CAC_nnn'GTG Cuts at: 2381 2437 2381 Size: 56 6089 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2072 2126 2418 Size: 54 1425 50 275 18 242 54 292 Cuts at: 2418 2565 2983 3429 3441 3592 4481 6110 8 Size: 147 418 446 12 151 889 1629 43 Fragments arranged by size: 1629 1425 889 446 418 292 275 242 151 147 54 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2126 2983 3441 3592 1537 Size: 275 314 857 458 151 4090 Fragments arranged by size: 4090 857 458 314 275 151 EarI CTCTTCn'nnn_ Cuts at: 1353 2394 4075 1353 Size: 1041 1681 3423 Fragments arranged by size: 3423 1681 1041 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4732 5106 5252 387 Size: 1064 138 3143 374 146 1280 Fragments arranged by size: 3143 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3082 3082 Size: 6145 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2418 2483 2602 2663 2855 2987 4208 Size: 393 786 65 119 61 192 132 1221 Cuts at: 4208 4805 5592 5860 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1221 787 786 597 393 268 192 132 119 65 61 EcoNI CCTnn'n_nnAGG Cuts at: 2504 2549 3092 2504 Size: 45 543 5557 Fragments arranged by size: 5557 543 45 EcoO109I rG'GnC_Cy Cuts at: 2824 3163 3402 2824 Size: 339 239 5567 Fragments arranged by size: 5567 339 239 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 FspI TGC'GCA Cuts at: 4651 4651 Size: 6145 HaeII r_GCGC'y Cuts at: 1678 2769 5292 1678 Size: 1091 2523 2531 Fragments arranged by size: 2531 2523 1091 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2351 2383 2762 2794 3022 Size: 32 135 32 779 32 379 32 228 Cuts at: 3022 3054 3119 3151 4786 4818 4860 4892 1373 Size: 32 65 32 1635 32 42 32 2626 Fragments arranged by size: 2626 1635 779 379 228 135 65 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3489 3735 669 Size: 988 281 1551 246 3079 Fragments arranged by size: 3079 1551 988 281 246 HindIII A'AGCT_T Cuts at: 748 3071 748 Size: 2323 3822 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3276 5259 5443 1305 Size: 1971 1983 184 2007 Fragments arranged by size: 2007 1983 1971 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2420 2567 10 Size: 54 1425 343 588 147 3588 Fragments arranged by size: 3588 1425 588 343 147 54 MslI CAynn'nnrTG Cuts at: 518 1166 3089 4103 4462 4621 518 Size: 648 1923 1014 359 159 2042 Fragments arranged by size: 2042 1923 1014 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2188 2232 3435 3561 4238 5386 5631 1606 Size: 582 44 1203 126 677 1148 245 2120 Fragments arranged by size: 2120 1203 1148 677 582 245 126 44 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5593 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 2126 3592 2126 Size: 1466 4679 NspI r_CATG'y Cuts at: 1132 1229 3503 5048 1132 Size: 97 2274 1545 2229 Fragments arranged by size: 2274 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6145 PciI A'CATG_T Cuts at: 5044 5044 Size: 6145 PflMI CCAn_nnn'nTGG Cuts at: 1108 2320 1108 Size: 1212 4933 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PmlI CAC'GTG Cuts at: 3250 3250 Size: 6145 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2720 2752 4214 4246 5727 5759 2720 Size: 32 1462 32 1481 32 3106 Fragments arranged by size: 3106 1481 1462 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2824 3163 2824 Size: 339 5806 PshAI GACnn'nnGTC Cuts at: 1613 2249 1613 Size: 636 5509 PsiI TTA'TAA Cuts at: 3715 3715 Size: 6145 PspOMI G'GGCC_C Cuts at: 2751 2751 Size: 6145 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2801 2833 2801 Size: 32 6113 PstI C_TGCA'G Cuts at: 830 2241 2613 3497 830 Size: 1411 372 884 3478 Fragments arranged by size: 3478 1411 884 372 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 2188 2188 Size: 6145 SacI G_AGCT'C Cuts at: 3084 3084 Size: 6145 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 2648 4393 6105 2648 Size: 1745 1712 2688 Fragments arranged by size: 2688 1745 1712 SexAI A'CCwGG_T Cuts at: 3308 3308 Size: 6145 SfcI C'TryA_G Cuts at: 826 1045 2237 2609 3493 4628 5309 5500 826 Size: 219 1192 372 884 1135 681 191 1471 Fragments arranged by size: 1471 1192 1135 884 681 372 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 2726 3472 1872 Size: 854 746 4545 Fragments arranged by size: 4545 854 746 SmlI C'TyrA_G Cuts at: 820 1017 1943 2090 2333 2546 2828 3155 4249 Size: 197 926 147 243 213 282 327 1094 Cuts at: 4249 5150 5412 820 Size: 901 262 1553 Fragments arranged by size: 1553 1094 926 901 327 282 262 243 213 197 147 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 3503 1132 Size: 2371 3774 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6145 StyI C'CwwG_G Cuts at: 513 1065 2568 3201 3584 513 Size: 552 1503 633 383 3074 Fragments arranged by size: 3074 1503 633 552 383 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2392 4172 4189 4342 4527 4972 325 Size: 1255 812 1780 17 153 185 445 1498 Fragments arranged by size: 1780 1498 1255 812 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2055 2646 4391 Size: 274 80 33 51 1443 78 591 1745 Cuts at: 4391 6103 96 Size: 1712 138 Fragments arranged by size: 1745 1712 1443 591 274 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2067 2099 3106 3138 6105 6137 2067 Size: 32 1007 32 2967 32 2075 Fragments arranged by size: 2967 2075 1007 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 3481 3481 Size: 6145 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2317 2575 2011 Size: 306 258 5581 Fragments arranged by size: 5581 306 258 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6145 XmaI C'CCGG_G Cuts at: 1870 2724 3470 1870 Size: 854 746 4545 Fragments arranged by size: 4545 854 746 XmnI GAAnn'nnTTC Cuts at: 4274 4274 Size: 6145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3953 276 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI