Restriction map for
pFN21AB5656
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspI AvrII BalI
BamHI BbvCI BcgI BciVI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoO65I
EcoRV EcoT22I FauI FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuII
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI TfiI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 68 enzymes) #
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AcyI 121 GGCGCC G[GA]^CG[CT]C
AhdI 374 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 436 CCTGAGG CC^T[ACGT]AGG
Aor51HI 449 AGCGCT AGC^GCT
Asp700I 355 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 374 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 703 GAGCTC G[AT]GC[AT]^C
AvaI 692 CTCGGG C^[CT]CG[GA]G
AviII 779 TGCGCA TGC^GCA
BanI 121 GGCGCC G^G[CT][GA]CC
BanII 703 GAGCTC G[GA]GC[CT]^C
BbeI 121 GGCGCC GGCGC^C
BbrPI 766 CACGTG CAC^GTG
BclI 606 TGATCA T^GATCA
BcnI 590 CCCGG CC^[GC]GG
BfaI 17 CTAG C^TAG
BglI 589 GCCCGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 703 GAGCTC G[AGT]GC[ACT]^C
BsaAI 766 CACGTG [CT]AC^GT[GA]
BsaHI 121 GGCGCC G[GA]^CG[CT]C
BsgI 958 CTGCAC GTGCAG(16/14)
BsiEI 1035 CGATCG CG[GA][CT]^CG
BsiHKAI 703 GAGCTC G[AT]GC[AT]^C
BsmFI 382 GTCCC GGGAC(10/14)
BsmI 747 GAATGC GAATGC(1/-1)
BsoBI 692 CTCGGG C^[CT]CG[GA]G
Bsp1286I 703 GAGCTC G[AGT]GC[ACT]^C
BspMI 867 GCAGGT ACCTGC(4/8)
BsrBI 820 GAGCGG CCGCTC(-3/-3)
BstYI 159 GGATCT [GA]^GATC[CT]
Bsu36I 436 CCTGAGG CC^T[ACGT]AGG
DdeI 437 CTGAG C^T[ACGT]AG
Eam1105I 374 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 287 CTCTTC CTCTTC(1/4)
Eco47III 449 AGCGCT AGC^GCT
Eco81I 436 CCTGAGG CC^T[ACGT]AGG
EcoNI 861 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 670 GAATTC G^AATTC
EcoT14I 59 CCAAGG C^C[AT][AT]GG
FbaI 606 TGATCA T^GATCA
FspI 779 TGCGCA TGC^GCA
HapII 591 CCGG C^CGG
HgaI 348 GCGTC GACGC(5/10)
Hin1I 121 GGCGCC G[GA]^CG[CT]C
HpaII 591 CCGG C^CGG
HphI 96 TCACC GGTGA(8/7)
KasI 121 GGCGCC G^GCGCC
Ksp632I 287 CTCTTC CTCTTC(1/4)
MaeI 17 CTAG C^TAG
MaeII 767 ACGT A^CGT
MaeIII 485 GTTAC ^GT[ACGT]AC
MflI 159 GGATCT [GA]^GATC[CT]
MseI 91 TTAA T^TAA
MspI 591 CCGG C^CGG
NarI 121 GGCGCC GG^CGCC
NciI 590 CCCGG CC^[GC]GG
PmaCI 766 CACGTG CAC^GTG
PmlI 766 CACGTG CAC^GTG
PvuI 1035 CGATCG CGAT^CG
SacI 703 GAGCTC GAGCT^C
SfaNI 583 GATGC GCATC(5/9)
SfoI 121 GGCGCC GGC^GCC
SmlI 167 CTCAAG C^T[CT][GA]AG
StuI 521 AGGCCT AGG^CCT
StyI 59 CCAAGG C^C[AT][AT]GG
Tru9I 91 TTAA T^TAA
XhoII 159 GGATCT [GA]^GATC[CT]
XmnI 355 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AcsI 148 GAATTT [GA]^AATT[CT]
670 GAATTC
AlwI 813 GATCC GGATC(4/5)
159 GGATC
AlwNI 847 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
868 CAGGTGCTG
ApoI 148 GAATTT [GA]^AATT[CT]
670 GAATTC
AvaII 260 GGTCC G^G[AT]CC
552 GGACC
BpmI 799 CTGGAG CTGGAG(16/14)
853 CTGGAG
BsaI 372 GAGACC GGTCTC(1/5)
856 GAGACC
BsaJI 59 CCAAGG C^C[ACGT][ACGT]GG
548 CCCAGG
BseRI 251 GAGGAG GAGGAG(10/8)
739 GAGGAG
BsrI 85 ACTGG ACTGG(1/-1)
498 ACTGG
Cfr13I 260 GGTCC G^G[ACGT]CC
552 GGACC
HaeII 121 GGCGCC [GA]GCGC^[CT]
449 AGCGCT
HaeIII 522 GGCC GG^CC
597 GGCC
NspI 776 GCATGC [GA]CATG^[CT]
893 GCATGC
Sau96I 260 GGTCC G^G[ACGT]CC
552 GGACC
SfcI 923 CTACAG C^T[GA][CT]AG
1007 CTACAG
SphI 776 GCATGC GCATG^C
893 GCATGC
Tsp509I 149 AATT ^AATT
671 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BbsI BbvI BpuAI
BsiYI BslI BsmAI BspHI BsrDI
BssKI BstF5I BstNI Cac8I CfoI
DpnII EcoRII Fnu4HI FokI HhaI
HinP1I HinfI ItaI MboI MboII
MnlI MslI MvaI MwoI NdeII
NlaIII NlaIV PleI RcaI Sau3AI
ScrFI TaqI TseI TspRI TthHB8I