Restriction map for
pFN21AB5656
(Linear) (Six-base) MAPSORT of: KIBB5656.seq Check: 527 from: 1 to: 1041
KIBB5656.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 858 1041
Size: 858 183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 519 1041
Size: 166 353 522
Fragments arranged by size:
522 353 166
AfeI AGC'GCT
Cuts at: 0 451 1041
Size: 451 590
AhdI GACnn_n'nnGTC
Cuts at: 0 379 1041
Size: 379 662
AleI CACnn'nnGTG
Cuts at: 0 787 1041
Size: 787 254
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 916 1041
Size: 916 125
AlwNI CAG_nnn'CTG
Cuts at: 0 852 873 1041
Size: 852 21 168
Fragments arranged by size:
852 168 21
ApoI r'AATT_y
Cuts at: 0 148 670 1041
Size: 148 522 371
Fragments arranged by size:
522 371 148
AvaI C'yCGr_G
Cuts at: 0 692 1041
Size: 692 349
BanI G'GyrC_C
Cuts at: 0 121 1041
Size: 121 920
BanII G_rGCy'C
Cuts at: 0 707 1041
Size: 707 334
BbeI G_GCGC'C
Cuts at: 0 125 1041
Size: 125 916
BbsI GAAGACnn'nnnn_
Cuts at: 0 56 185 525 1041
Size: 56 129 340 516
Fragments arranged by size:
516 340 129 56
BclI T'GATC_A
Cuts at: 0 606 1041
Size: 606 435
BglI GCCn_nnn'nGGC
Cuts at: 0 595 1041
Size: 595 446
BmrI ACTGGGnnnn_n'
Cuts at: 0 95 1041
Size: 95 946
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 180 1041
Size: 180 861
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 874 1041
Size: 820 54 167
Fragments arranged by size:
820 167 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 1041
Size: 152 889
BsaI GGTCTCn'nnnn_
Cuts at: 0 366 850 1041
Size: 366 484 191
Fragments arranged by size:
484 366 191
BsaAI yAC'GTr
Cuts at: 0 768 1041
Size: 768 273
BsaHI Gr'CG_yC
Cuts at: 0 122 1041
Size: 122 919
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 266 754 1041
Size: 266 488 287
Fragments arranged by size:
488 287 266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 1041
Size: 943 98
BsiEI CG_ry'CG
Cuts at: 0 1038 1041
Size: 1038 3
BsiHKAI G_wGCw'C
Cuts at: 0 707 1041
Size: 707 334
BsmI GAATG_Cn'
Cuts at: 0 753 1041
Size: 753 288
Bsp1286I G_dGCh'C
Cuts at: 0 707 1041
Size: 707 334
BspHI T'CATG_A
Cuts at: 0 338 603 609 1041
Size: 338 265 6 432
Fragments arranged by size:
432 338 265 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 858 1041
Size: 858 183
BsrBI CCG'CTC
Cuts at: 0 822 1041
Size: 822 219
BsrDI GCAATG_nn'
Cuts at: 0 219 306 420 1041
Size: 219 87 114 621
Fragments arranged by size:
621 219 114 87
BstYI r'GATC_y
Cuts at: 0 159 1041
Size: 159 882
Bsu36I CC'TnA_GG
Cuts at: 0 437 1041
Size: 437 604
BtsI GCAGTG_nn'
Cuts at: 0 794 1041
Size: 794 247
EarI CTCTTCn'nnn_
Cuts at: 0 293 1041
Size: 293 748
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 697 1041
Size: 697 344
EcoICRI GAG'CTC
Cuts at: 0 705 1041
Size: 705 336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 519 820 874 1041
Size: 166 353 301 54 167
Fragments arranged by size:
353 301 167 166 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 865 1041
Size: 865 176
EcoRI G'AATT_C
Cuts at: 0 670 1041
Size: 670 371
FspI TGC'GCA
Cuts at: 0 781 1041
Size: 781 260
FspAI rTGC'GCAy
Cuts at: 0 781 1041
Size: 781 260
HaeII r_GCGC'y
Cuts at: 0 125 453 1041
Size: 125 328 588
Fragments arranged by size:
588 328 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 57 89 365 397 1041
Size: 57 32 276 32 644
Fragments arranged by size:
644 276 57 32 32
KasI G'GCGC_C
Cuts at: 0 121 1041
Size: 121 920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1041
Size: 749 292
MslI CAynn'nnrTG
Cuts at: 0 230 403 608 787 1041
Size: 230 173 205 179 254
Fragments arranged by size:
254 230 205 179 173
NarI GG'CG_CC
Cuts at: 0 122 1041
Size: 122 919
NspI r_CATG'y
Cuts at: 0 780 897 1041
Size: 780 117 144
Fragments arranged by size:
780 144 117
PfoI T'CCnGG_A
Cuts at: 0 815 1041
Size: 815 226
PmlI CAC'GTG
Cuts at: 0 768 1041
Size: 768 273
PvuI CG_AT'CG
Cuts at: 0 1038 1041
Size: 1038 3
SacI G_AGCT'C
Cuts at: 0 707 1041
Size: 707 334
SfcI C'TryA_G
Cuts at: 0 923 1007 1041
Size: 923 84 34
Fragments arranged by size:
923 84 34
SfoI GGC'GCC
Cuts at: 0 123 1041
Size: 123 918
SmlI C'TyrA_G
Cuts at: 0 167 1041
Size: 167 874
SphI G_CATG'C
Cuts at: 0 780 897 1041
Size: 780 117 144
Fragments arranged by size:
780 144 117
StuI AGG'CCT
Cuts at: 0 523 1041
Size: 523 518
StyI C'CwwG_G
Cuts at: 0 59 1041
Size: 59 982
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 113 321 1041
Size: 113 208 720
Fragments arranged by size:
720 208 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 126 158 1041
Size: 126 32 883
Fragments arranged by size:
883 126 32
XmnI GAAnn'nnTTC
Cuts at: 0 359 1041
Size: 359 682
Enzymes that do cut:
AarI AcuI AfeI AhdI AleI AlfI AlwNI ApoI
AvaI BanI BanII BbeI BbsI BclI BglI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaHI BseRI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BstYI Bsu36I BtsI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI FspI FspAI HaeII Hin4I KasI MmeI MslI
NarI NspI PfoI PmlI PvuI SacI SfcI SfoI
SmlI SphI StuI StyI TaqII TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BfrBI BglII BlpI Bme1580I BmgBI
BmtI Bpu10I BsaBI BsaWI BsaXI BseYI BsiWI BsmBI
BspEI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EcoO109I EcoRV FalI FseI HincII
HindIII HpaI KpnI MfeI MluI MscI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI ZraI