Restriction map for pFN21AB5643
 (Circular) (Six-base) MAPSORT of: pFN21AB5643.seq  Check: 3053  from: 1  to: 4921



pFN21AB5643  4921 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:25  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2731     278 
   Size:         53      83     186     936    1195    2468

  Fragments arranged by size: 

                2468    1195     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2264    1937 
   Size:        327    4594
 
Acc65I G'GTAC_C

Cuts at:   2242    2242 
   Size:       4921
 
AclI AA'CG_TT

Cuts at:   1563    3048    3421    1563 
   Size:       1485     373    3063

  Fragments arranged by size: 

                3063    1485     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2163    2984    4368    4636    1632 
   Size:        531     821    1384     268    1917

  Fragments arranged by size: 

                1917    1384     821     531     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4724
 
AflIII A'CryG_T

Cuts at:   1203    3820    4797    1203 
   Size:       2617     977    1327

  Fragments arranged by size: 

                2617    1327     977
 
AgeI A'CCGG_T

Cuts at:   3752    3752 
   Size:       4921
 
AhdI GACnn_n'nnGTC

Cuts at:   2122    3650    2122 
   Size:       1528    3393
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       4921
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       4921
 
AlwNI CAG_nnn'CTG

Cuts at:   2664    4236    2664 
   Size:       1572    3349
 
ApaLI G'TGCA_C

Cuts at:   2978    4134    2978 
   Size:       1156    3765
 
ApoI r'AATT_y

Cuts at:   1493    2230    2456    2608    4717    1493 
   Size:        737     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     737     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3475     160 
   Size:        872    2443    1606

  Fragments arranged by size: 

                2443    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       4921
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2246    1870 
   Size:         73     303    4545

  Fragments arranged by size: 

                4545     303      73
 
BamHI G'GATC_C

Cuts at:   2251    2618    2251 
   Size:        367    4554
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2242    3697     618 
   Size:        325     217     627     455    1455    1842

  Fragments arranged by size: 

                1842    1455     627     455     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2240    1391 
   Size:        849    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    3950
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3112    3146    1903 
   Size:         34    1175      34    3678

  Fragments arranged by size: 

                3678    1175      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2813    4023    2813 
   Size:       1210    3711
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       4921
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3532     136 
   Size:        107     122      71     631     605    1860    1525

  Fragments arranged by size: 

                1860    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   4916    4916 
   Size:       4921
 
BlpI GC'TnA_GC

Cuts at:   2338    2338 
   Size:       4921
 
Bme1580I G_kGCm'C

Cuts at:   1790    2982    4138    1790 
   Size:       1192    1156    2573

  Fragments arranged by size: 

                2573    1192    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       4921
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2652    3610     442 
   Size:        416      53      83     399      24    1235     958    1753

  Fragments arranged by size: 

                1753    1235     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       4921
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3581    1239 
   Size:       2342    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3046    3911    4209    3046 
   Size:        865     298    3758

  Fragments arranged by size: 

                3758     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3584     882 
   Size:        617    2085    2219

  Fragments arranged by size: 

                2219    2085     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2712     493 
   Size:        711    1508    2702

  Fragments arranged by size: 

                2702    1508     711
 
BsaBI GATnn'nnATC

Cuts at:   4915    4915 
   Size:       4921
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2728    3110    3745     275 
   Size:         53      83     186     936    1195     382     635    1451

  Fragments arranged by size: 

                1451    1195     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3354    3752    4026    4173    4584    3354 
   Size:        398     274     147     411    3691

  Fragments arranged by size: 

                3691     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4696

  Fragments arranged by size: 

                4696     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       4921
 
BseYI C'CCAG_C

Cuts at:   4124    4620    4124 
   Size:        496    4425
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2078    1163 
   Size:        573     342    4006

  Fragments arranged by size: 

                4006     573     342
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2371    3132    4160    1540 
   Size:        275     186     370     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     370     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2982    3067    4138    2982 
   Size:         85    1071    3765

  Fragments arranged by size: 

                3765    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2432    2525    2432 
   Size:         93    4828
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2240    2982    3067    4138    1391 
   Size:        399     450     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     450     399      85
 
BspEI T'CCGG_A

Cuts at:   4584    4584 
   Size:       4921
 
BspHI T'CATG_A

Cuts at:   2810    4540    2810 
   Size:       1730    3191
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2276     844 
   Size:       1432    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2808    1394 
   Size:        429     985    3507

  Fragments arranged by size: 

                3507     985     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3416    3590      65 
   Size:       1183    2168     174    1396

  Fragments arranged by size: 

                2168    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3565    3752    1700 
   Size:        110    1755     187    2869

  Fragments arranged by size: 

                2869    1755     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       4921
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       4921
 
BssSI C'ACGA_G

Cuts at:   2981    3993    2981 
   Size:       1012    3909
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       4921
 
BstBI TT'CG_AA

Cuts at:   2102    3725    2102 
   Size:       1623    3298
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       4921
 
BstYI r'GATC_y

Cuts at:   1972    2251    2618    3003    3020    4472    4558    4570    4916 
   Size:        279     367     385      17    1452      86      12     346

Cuts at:   4916    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     385     367     346     279      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4369
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4182

  Fragments arranged by size: 

                4182     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2437    3231    3251    2437 
   Size:        794      20    4107

  Fragments arranged by size: 

                4107     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       4921
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    4886
 
DraI TTT'AAA

Cuts at:   2225    2225 
   Size:       4921
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3928     809 
   Size:        507    2612    1802

  Fragments arranged by size: 

                2612    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2368    3257    4886 
   Size:         54    1425      50     275      18     538     889    1629

Cuts at:   4886       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     538     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2368    1537 
   Size:        275     556    4090

  Fragments arranged by size: 

                4090     556     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2851    1353 
   Size:       1498    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3508    3882    4028     387 
   Size:       1064     138    1919     374     146    1280

  Fragments arranged by size: 

                1919    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2163    2984    3581    4368    4636    1239 
   Size:        393     531     821     597     787     268    1524

  Fragments arranged by size: 

                1524     821     787     597     531     393     268
 
EcoRI G'AATT_C

Cuts at:   2230    2608    2230 
   Size:        378    4543
 
FspI TGC'GCA

Cuts at:   3427    3427 
   Size:       4921
 
HaeII r_GCGC'y

Cuts at:   1678    4068    1678 
   Size:       2390    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2108    2140    3562    3594    3636 
   Size:         32     135      32     536      32    1422      32      42

Cuts at:   3636    3668    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1422     536     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2265    2511     669 
   Size:        988     281     327     246    3079

  Fragments arranged by size: 

                3079     988     327     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       4921
 
HpaI GTT'AAC

Cuts at:   2511    2511 
   Size:       4921
 
KpnI G_GTAC'C

Cuts at:   2246    2246 
   Size:       4921
 
MfeI C'AATT_G

Cuts at:   2520    2520 
   Size:       4921
 
MluI A'CGCG_T

Cuts at:   4797    4797 
   Size:       4921
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4035    4219    1305 
   Size:       2730     184    2007

  Fragments arranged by size: 

                2730    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3099

  Fragments arranged by size: 

                3099    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2879    3238    3397     518 
   Size:        648    1713     359     159    2042

  Fragments arranged by size: 

                2042    1713     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2114    2337    3014    4162    4407    1606 
   Size:        508     223     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     508     245     223
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4369
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       4921
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       4921
 
NotI GC'GGCC_GC

Cuts at:   2368    2368 
   Size:       4921
 
NspI r_CATG'y

Cuts at:   1132    1229    2144    2279    3824    1132 
   Size:         97     915     135    1545    2229

  Fragments arranged by size: 

                2229    1545     915     135      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       4921
 
PciI A'CATG_T

Cuts at:   3820    3820 
   Size:       4921
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       4921
 
PmeI GTTT'AAAC

Cuts at:   2225    2225 
   Size:       4921
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2990    3022    4503    4535    2990 
   Size:         32    1481      32    3376

  Fragments arranged by size: 

                3376    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       4921
 
PsiI TTA'TAA

Cuts at:   2491    2491 
   Size:       4921
 
PstI C_TGCA'G

Cuts at:    830    2273     830 
   Size:       1443    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       4921
 
SalI G'TCGA_C

Cuts at:   1936    2263    1936 
   Size:        327    4594
 
SbfI CC_TGCA'GG

Cuts at:   2273    2273 
   Size:       4921
 
ScaI AGT'ACT

Cuts at:   3169    4881    3169 
   Size:       1712    3209
 
SfcI C'TryA_G

Cuts at:    826    1045    2269    3404    4085    4276     826 
   Size:        219    1224    1135     681     191    1471

  Fragments arranged by size: 

                1471    1224    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       4921
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       4921
 
SmaI CCC'GGG

Cuts at:   1872    2248    1872 
   Size:        376    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3025    3926    4188     820 
   Size:        197     926    1082     901     262    1553

  Fragments arranged by size: 

                1553    1082     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2712     493 
   Size:       2219    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       4921
 
SphI G_CATG'C

Cuts at:   1132    2279    1132 
   Size:       1147    3774
 
SspI AAT'ATT

Cuts at:      5      52    2845       5 
   Size:         47    2793    2081

  Fragments arranged by size: 

                2793    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       4921
 
StyI C'CwwG_G

Cuts at:    513    1065    2360     513 
   Size:        552    1295    3074

  Fragments arranged by size: 

                3074    1295     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2948    2965    3118    3303    3748     325 
   Size:       1255    1368      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1368    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3167    4879      96 
   Size:        274      80      33      51    1443    1190    1712     138

  Fragments arranged by size: 

                1712    1443    1190     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4881    4913    4881 
   Size:         32    4889
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       4921
 
XbaI T'CTAG_A

Cuts at:   2257    2257 
   Size:       4921
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       4921
 
XmaI C'CCGG_G

Cuts at:   1870    2246    1870 
   Size:        376    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3050    3050 
   Size:       4921
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2729     276 
   Size:         53      83     186     936    1195    2468

  Fragments arranged by size: 

                2468    1195     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI    PshAI
     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI
     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
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