(Circular) (Six-base) MAPSORT of: pFN21AB5523.seq Check: 1389 from: 1 to: 12121 pFN21AB5523 12121 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3427 5197 3427 Size: 1770 10351 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6610 9931 278 Size: 53 83 186 936 5074 3321 2468 Fragments arranged by size: 5074 3321 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 9464 1937 Size: 7527 4594 Acc65I G'GTAC_C Cuts at: 9442 9442 Size: 12121 AclI AA'CG_TT Cuts at: 1563 9420 10248 10621 1563 Size: 7857 828 373 3063 Fragments arranged by size: 7857 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2180 2184 2234 2295 2547 2744 3213 4811 Size: 548 4 50 61 252 197 469 1598 Cuts at: 4811 5444 5487 5502 7166 7589 7751 7925 10184 Size: 633 43 15 1664 423 162 174 2259 Cuts at: 10184 11568 11836 1632 Size: 1384 268 1917 Fragments arranged by size: 2259 1917 1664 1598 1384 633 548 469 423 268 252 197 174 162 61 50 43 15 4 AfeI AGC'GCT Cuts at: 2780 3516 5645 6032 2780 Size: 736 2129 387 8869 Fragments arranged by size: 8869 2129 736 387 AflII C'TTAA_G Cuts at: 820 1017 3521 820 Size: 197 2504 9420 Fragments arranged by size: 9420 2504 197 AflIII A'CryG_T Cuts at: 1203 8146 11020 11997 1203 Size: 6943 2874 977 1327 Fragments arranged by size: 6943 2874 1327 977 AgeI A'CCGG_T Cuts at: 10952 10952 Size: 12121 AhdI GACnn_n'nnGTC Cuts at: 5476 6058 7333 10850 5476 Size: 582 1275 3517 6747 Fragments arranged by size: 6747 3517 1275 582 AleI CACnn'nnGTG Cuts at: 1166 3225 1166 Size: 2059 10062 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3425 4707 4885 5150 6338 6540 8930 1184 Size: 2241 1282 178 265 1188 202 2390 4375 Fragments arranged by size: 4375 2390 2241 1282 1188 265 202 178 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 9200 9232 9200 Size: 32 12089 AlwNI CAG_nnn'CTG Cuts at: 2122 2545 2722 2881 3283 4342 4468 4660 5665 Size: 423 177 159 402 1059 126 192 1005 Cuts at: 5665 5815 6217 6964 7408 7537 7600 8209 9301 Size: 150 402 747 444 129 63 609 1092 Cuts at: 9301 9864 11436 2122 Size: 563 1572 2807 Fragments arranged by size: 2807 1572 1092 1059 1005 747 609 563 444 423 402 402 192 177 159 150 129 126 63 ApaI G_GGCC'C Cuts at: 3706 9250 3706 Size: 5544 6577 ApaLI G'TGCA_C Cuts at: 5944 10178 11334 5944 Size: 4234 1156 6731 Fragments arranged by size: 6731 4234 1156 ApoI r'AATT_y Cuts at: 1493 2573 3587 8984 9430 9656 9808 11917 1493 Size: 1080 1014 5397 446 226 152 2109 1697 Fragments arranged by size: 5397 2109 1697 1080 1014 446 226 152 AseI AT'TA_AT Cuts at: 160 1032 10675 160 Size: 872 9643 1606 Fragments arranged by size: 9643 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12121 AvaI C'yCGr_G Cuts at: 1870 1943 4953 5097 5915 6393 7587 9306 9446 Size: 73 3010 144 818 478 1194 1719 140 Cuts at: 9446 1870 Size: 4545 Fragments arranged by size: 4545 3010 1719 1194 818 478 144 140 73 BamHI G'GATC_C Cuts at: 6745 6819 8664 9139 9451 9818 6745 Size: 74 1845 475 312 367 9048 Fragments arranged by size: 9048 1845 475 367 312 74 BanI G'GyrC_C Cuts at: 618 943 1160 1787 9442 10897 618 Size: 325 217 627 7655 1455 1842 Fragments arranged by size: 7655 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3706 3759 3867 4320 4419 7370 9250 9291 Size: 2315 53 108 453 99 2951 1880 41 Cuts at: 9291 9372 9440 1391 Size: 81 68 4072 Fragments arranged by size: 4072 2951 2315 1880 453 108 99 81 68 53 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2934 3252 3489 3540 4776 5475 5934 Size: 971 1035 318 237 51 1236 699 459 Cuts at: 5934 6423 6474 8009 8022 928 Size: 489 51 1535 13 5027 Fragments arranged by size: 5027 1535 1236 1035 971 699 489 459 318 237 51 51 13 BbvCI CC'TCA_GC Cuts at: 2952 4779 6126 8423 9251 2952 Size: 1827 1347 2297 828 5822 Fragments arranged by size: 5822 2297 1827 1347 828 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4037 4071 4757 4791 7174 7208 7691 Size: 34 2100 34 686 34 2383 34 483 Cuts at: 7691 7725 10312 10346 1903 Size: 34 2587 34 3678 Fragments arranged by size: 3678 2587 2383 2100 686 483 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2175 10013 11223 2175 Size: 7838 1210 3073 Fragments arranged by size: 7838 3073 1210 BclI T'GATC_A Cuts at: 1548 2679 3972 6453 9189 1548 Size: 1131 1293 2481 2736 4480 Fragments arranged by size: 4480 2736 2481 1293 1131 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4292 6398 6902 Size: 107 122 71 631 605 2620 2106 504 Cuts at: 6902 7046 8168 8300 9245 10732 136 Size: 144 1122 132 945 1487 1525 Fragments arranged by size: 2620 2106 1525 1487 1122 945 631 605 504 144 132 122 107 71 BglII A'GATC_T Cuts at: 2911 4129 4816 12116 2911 Size: 1218 687 7300 2916 Fragments arranged by size: 7300 2916 1218 687 BlpI GC'TnA_GC Cuts at: 3861 9538 3861 Size: 5677 6444 Bme1580I G_kGCm'C Cuts at: 1790 3559 3706 5948 9250 10182 11338 1790 Size: 1769 147 2242 3302 932 1156 2573 Fragments arranged by size: 3302 2573 2242 1769 1156 932 147 BmgBI CAC'GTC Cuts at: 1318 2485 4398 7963 1318 Size: 1167 1913 3565 5476 Fragments arranged by size: 5476 3565 1913 1167 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3040 4914 5080 Size: 416 53 83 399 24 1623 1874 166 Cuts at: 5080 5249 9852 10810 442 Size: 169 4603 958 1753 Fragments arranged by size: 4603 1874 1753 1623 958 416 399 169 166 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3713 6107 1056 Size: 2657 2394 7070 Fragments arranged by size: 7070 2657 2394 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7861 7861 Size: 12121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 2567 2924 3383 4136 4682 5132 5375 Size: 953 375 357 459 753 546 450 243 Cuts at: 5375 5495 5986 6158 6314 7907 8264 8549 8560 Size: 120 491 172 156 1593 357 285 11 Cuts at: 8560 8597 8948 10781 1239 Size: 37 351 1833 2579 Fragments arranged by size: 2579 1833 1593 953 753 546 491 459 450 375 357 357 351 285 243 172 156 120 37 11 Bpu10I CC'TnA_GC Cuts at: 2952 4779 6038 6126 6910 8423 9251 2952 Size: 1827 1259 88 784 1513 828 5822 Fragments arranged by size: 5822 1827 1513 1259 828 784 88 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3743 4295 10246 11111 11409 3743 Size: 552 5951 865 298 4455 Fragments arranged by size: 5951 4455 865 552 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2853 8016 8528 8666 10784 882 Size: 617 1354 5163 512 138 2118 2219 Fragments arranged by size: 5163 2219 2118 1354 617 512 138 BsaAI yAC'GTr Cuts at: 493 1204 5065 9274 9349 9391 9912 493 Size: 711 3861 4209 75 42 521 2702 Fragments arranged by size: 4209 3861 2702 711 521 75 42 BsaBI GATnn'nnATC Cuts at: 2133 12115 2133 Size: 9982 2139 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2113 6607 8406 9928 Size: 53 83 186 936 580 4494 1799 1522 Cuts at: 9928 10310 10945 275 Size: 382 635 1451 Fragments arranged by size: 4494 1799 1522 1451 936 635 580 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6119 6156 6816 10554 10952 11226 11373 11784 6119 Size: 37 660 3738 398 274 147 411 6456 Fragments arranged by size: 6456 3738 660 411 398 274 147 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2286 2316 9314 9344 1178 Size: 30 165 30 883 30 6998 30 3955 Fragments arranged by size: 6998 3955 883 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2684 3359 4406 5129 5369 5513 7346 7535 Size: 1498 675 1047 723 240 144 1833 189 Cuts at: 7535 7850 8036 8351 8407 8546 8621 8678 8837 Size: 315 186 315 56 139 75 57 159 Cuts at: 8837 8984 9239 9299 1186 Size: 147 255 60 4008 Fragments arranged by size: 4008 1833 1498 1047 723 675 315 315 255 240 189 186 159 147 144 139 75 60 57 56 BseYI C'CCAG_C Cuts at: 2533 3129 3185 3705 4644 4680 8072 8637 8733 Size: 596 56 520 939 36 3392 565 96 Cuts at: 8733 8877 8902 11324 11820 2533 Size: 144 25 2422 496 2834 Fragments arranged by size: 3392 2834 2422 939 596 565 520 496 144 96 56 36 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 2369 2480 4163 5525 6006 6306 Size: 573 564 69 111 1683 1362 481 300 Cuts at: 6306 7286 7421 7777 8057 1163 Size: 980 135 356 280 5227 Fragments arranged by size: 5227 1683 1362 980 573 564 481 356 300 280 135 111 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5038 7384 9571 10332 11360 1540 Size: 275 186 3037 2346 2187 761 1028 2301 Fragments arranged by size: 3037 2346 2301 2187 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3222 3759 3867 5391 5948 7557 8859 9291 9372 Size: 537 108 1524 557 1609 1302 432 81 Cuts at: 9372 10182 10267 11338 3222 Size: 810 85 1071 4005 Fragments arranged by size: 4005 1609 1524 1302 1071 810 557 537 432 108 85 81 BsiWI C'GTAC_G Cuts at: 5061 5061 Size: 12121 BsmI GAATG_Cn' Cuts at: 4552 9632 9725 4552 Size: 5080 93 6948 Fragments arranged by size: 6948 5080 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4388 9334 4388 Size: 4946 7175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3222 3559 3706 3759 3867 4320 4419 Size: 399 1432 337 147 53 108 453 99 Cuts at: 4419 5391 5948 7370 7557 8859 9250 9291 9372 Size: 972 557 1422 187 1302 391 41 81 Cuts at: 9372 9440 10182 10267 11338 1391 Size: 68 742 85 1071 2174 Fragments arranged by size: 2174 1432 1422 1302 1071 972 742 557 453 399 391 337 187 147 108 99 85 81 68 53 41 BspEI T'CCGG_A Cuts at: 11784 11784 Size: 12121 BspHI T'CATG_A Cuts at: 2605 8187 9186 10010 11740 2605 Size: 5582 999 824 1730 2986 Fragments arranged by size: 5582 2986 1730 999 824 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2056 3417 3427 5197 5368 5940 6033 7098 Size: 1212 1361 10 1770 171 572 93 1065 Cuts at: 7098 9087 9476 844 Size: 1989 389 3489 Fragments arranged by size: 3489 1989 1770 1361 1212 1065 572 389 171 93 10 BsrBI CCG'CTC Cuts at: 1394 1823 3353 5342 8415 9405 10008 1394 Size: 429 1530 1989 3073 990 603 3507 Fragments arranged by size: 3507 3073 1989 1530 990 603 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3630 3676 4066 4419 5324 6131 6589 Size: 1183 2382 46 390 353 905 807 458 Cuts at: 6589 8100 10616 10790 65 Size: 1511 2516 174 1396 Fragments arranged by size: 2516 2382 1511 1396 1183 905 807 458 390 353 174 46 BsrFI r'CCGG_y Cuts at: 1700 1810 4872 5053 5180 6545 6576 6872 7456 Size: 110 3062 181 127 1365 31 296 584 Cuts at: 7456 10765 10952 1700 Size: 3309 187 2869 Fragments arranged by size: 3309 3062 2869 1365 584 296 187 181 127 110 31 BsrGI T'GTAC_A Cuts at: 96 96 Size: 12121 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12121 BssSI C'ACGA_G Cuts at: 2894 3680 4289 4601 10181 11193 2894 Size: 786 609 312 5580 1012 3822 Fragments arranged by size: 5580 3822 1012 786 609 312 BstAPI GCAn_nnn'nTGC Cuts at: 794 3287 4502 5512 7108 794 Size: 2493 1215 1010 1596 5807 Fragments arranged by size: 5807 2493 1596 1215 1010 BstBI TT'CG_AA Cuts at: 10925 10925 Size: 12121 BstEII G'GTnAC_C Cuts at: 1184 4960 1184 Size: 3776 8345 BstXI CCAn_nnnn'nTGG Cuts at: 3181 4244 3181 Size: 1063 11058 BstYI r'GATC_y Cuts at: 1972 2542 2911 3039 3811 4129 4816 6745 6819 Size: 570 369 128 772 318 687 1929 74 Cuts at: 6819 7524 8664 8937 9139 9451 9818 10203 10220 Size: 705 1140 273 202 312 367 385 17 Cuts at: 10220 11672 11758 11770 12116 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1929 1452 1140 772 705 687 570 385 369 367 346 318 312 273 202 128 86 74 17 12 Bsu36I CC'TnA_GG Cuts at: 2108 2667 7409 2108 Size: 559 4742 6820 Fragments arranged by size: 6820 4742 559 BtgI C'CryG_G Cuts at: 513 1065 2004 4320 4551 4890 5576 5607 6823 Size: 552 939 2316 231 339 686 31 1216 Cuts at: 6823 8517 8817 8865 9084 513 Size: 1694 300 48 219 3550 Fragments arranged by size: 3550 2316 1694 1216 939 686 552 339 300 231 219 48 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6523 7280 487 Size: 683 56 5297 757 5328 Fragments arranged by size: 5328 5297 757 683 56 BtsI GCAGTG_nn' Cuts at: 3281 3404 5132 5191 6883 7192 8112 8888 9637 Size: 123 1728 59 1692 309 920 776 749 Cuts at: 9637 10431 10451 3281 Size: 794 20 4951 Fragments arranged by size: 4951 1728 1692 920 794 776 749 309 123 59 20 ClaI AT'CG_AT Cuts at: 1554 2137 6168 1554 Size: 583 4031 7507 Fragments arranged by size: 7507 4031 583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 12086 DraI TTT'AAA Cuts at: 9425 9425 Size: 12121 DraIII CAC_nnn'GTG Cuts at: 5608 5608 Size: 12121 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7763 9326 11128 809 Size: 507 6447 1563 1802 1802 Fragments arranged by size: 6447 1802 1802 1563 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2854 3636 3907 Size: 54 1425 50 275 18 1024 782 271 Cuts at: 3907 4246 4344 5610 5667 6547 7381 7683 8017 Size: 339 98 1266 57 880 834 302 334 Cuts at: 8017 8211 8454 9154 9568 10457 12086 8 Size: 194 243 700 414 889 1629 43 Fragments arranged by size: 1629 1425 1266 1024 889 880 834 782 700 414 339 334 302 275 271 243 194 98 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7381 9568 1537 Size: 275 5569 2187 4090 Fragments arranged by size: 5569 4090 2187 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2160 2523 2574 3422 4376 4940 5264 5748 Size: 807 363 51 848 954 564 324 484 Cuts at: 5748 6492 7599 7638 7852 8186 8285 8706 10051 Size: 744 1107 39 214 334 99 421 1345 Cuts at: 10051 1353 Size: 3423 Fragments arranged by size: 3423 1345 1107 954 848 807 744 564 484 421 363 334 324 214 99 51 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7973 10708 11082 11228 387 Size: 1064 138 6384 2735 374 146 1280 Fragments arranged by size: 6384 2735 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3757 3865 9289 9370 3757 Size: 108 5424 81 6508 Fragments arranged by size: 6508 5424 108 81 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2184 2192 2234 2295 2547 2567 Size: 393 548 4 8 42 61 252 20 Cuts at: 2567 2744 2924 3213 3383 4136 4682 4811 5132 Size: 177 180 289 170 753 546 129 321 Cuts at: 5132 5375 5444 5487 5495 5502 5986 6158 6314 Size: 243 69 43 8 7 484 172 156 Cuts at: 6314 7166 7589 7751 7907 7925 8264 8549 8560 Size: 852 423 162 156 18 339 285 11 Cuts at: 8560 8597 8948 10184 10781 11568 11836 1239 Size: 37 351 1236 597 787 268 1524 Fragments arranged by size: 1524 1236 852 787 753 597 548 546 484 423 393 351 339 321 289 285 268 252 243 180 177 172 170 162 156 156 129 69 61 43 42 37 20 18 11 8 8 7 4 EcoNI CCTnn'n_nnAGG Cuts at: 3341 4172 6296 3341 Size: 831 2124 9166 Fragments arranged by size: 9166 2124 831 EcoO109I rG'GnC_Cy Cuts at: 2068 2728 4657 5419 5812 5845 6052 7039 7150 Size: 660 1929 762 393 33 207 987 111 Cuts at: 7150 7387 7861 8275 8524 8697 8721 8803 8827 Size: 237 474 414 249 173 24 82 24 Cuts at: 8827 8974 9247 2068 Size: 147 273 4942 Fragments arranged by size: 4942 1929 987 762 660 474 414 393 273 249 237 207 173 147 111 82 33 24 24 EcoRI G'AATT_C Cuts at: 8984 9430 9808 8984 Size: 446 378 11297 Fragments arranged by size: 11297 446 378 EcoRV GAT'ATC Cuts at: 4981 4981 Size: 12121 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3536 8147 3536 Size: 4611 7510 FspI TGC'GCA Cuts at: 3380 7449 8912 10627 3380 Size: 4069 1463 1715 4874 Fragments arranged by size: 4874 4069 1715 1463 FspAI rTGC'GCAy Cuts at: 8912 8912 Size: 12121 HaeII r_GCGC'y Cuts at: 1678 2782 3359 3518 5647 5700 5899 6034 7240 Size: 1104 577 159 2129 53 199 135 1206 Cuts at: 7240 9240 11268 1678 Size: 2000 2028 2531 Fragments arranged by size: 2531 2129 2028 2000 1206 1104 577 199 159 135 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4697 4729 4757 4789 4801 Size: 32 135 32 3125 32 28 32 12 Cuts at: 4801 4833 5140 5172 5462 5494 5781 5813 6044 Size: 32 307 32 290 32 287 32 231 Cuts at: 6044 6076 6313 6345 6496 6528 6620 6652 7319 Size: 32 237 32 151 32 92 32 667 Cuts at: 7319 7351 7794 7826 7913 7945 9035 9067 10762 Size: 32 443 32 87 32 1090 32 1695 Cuts at: 10762 10794 10836 10868 1373 Size: 32 42 32 2626 Fragments arranged by size: 3125 2626 1695 1090 667 443 307 290 287 237 231 151 135 92 87 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 28 12 HincII GTy'rAC Cuts at: 669 1657 1938 3928 9465 9711 669 Size: 988 281 1990 5537 246 3079 Fragments arranged by size: 5537 3079 1990 988 281 246 HindIII A'AGCT_T Cuts at: 748 2246 3801 4022 4595 4638 4803 7743 8237 Size: 1498 1555 221 573 43 165 2940 494 Cuts at: 8237 8385 748 Size: 148 4484 Fragments arranged by size: 4484 2940 1555 1498 573 494 221 165 148 43 HpaI GTT'AAC Cuts at: 9711 9711 Size: 12121 KpnI G_GTAC'C Cuts at: 9446 9446 Size: 12121 MfeI C'AATT_G Cuts at: 2514 9720 2514 Size: 7206 4915 MluI A'CGCG_T Cuts at: 11997 11997 Size: 12121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2792 3843 5244 5341 6688 8520 11235 11419 Size: 1487 1051 1401 97 1347 1832 2715 184 Cuts at: 11419 1305 Size: 2007 Fragments arranged by size: 2715 2007 1832 1487 1401 1347 1051 184 97 MscI TGG'CCA Cuts at: 10 64 1489 1832 3638 3909 4248 5612 7685 Size: 54 1425 343 1806 271 339 1364 2073 Cuts at: 7685 8213 9156 10 Size: 528 943 2975 Fragments arranged by size: 2975 2073 1806 1425 1364 943 528 343 339 271 54 MslI CAynn'nnrTG Cuts at: 518 1166 3225 4254 5724 8912 10079 10438 10597 Size: 648 2059 1029 1470 3188 1167 359 159 Cuts at: 10597 518 Size: 2042 Fragments arranged by size: 3188 2059 2042 1470 1167 1029 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2719 3129 5665 6217 6349 6947 7534 7576 Size: 1113 410 2536 552 132 598 587 42 Cuts at: 7576 7583 7597 8319 8452 8596 8893 8902 9537 Size: 7 14 722 133 144 297 9 635 Cuts at: 9537 10214 11362 11607 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2536 2120 1148 1113 722 677 635 598 587 552 410 297 245 144 133 132 42 14 9 7 NaeI GCC'GGC Cuts at: 4874 5055 5182 6547 6578 6874 7458 4874 Size: 181 127 1365 31 296 584 9537 Fragments arranged by size: 9537 1365 584 296 181 127 31 NcoI C'CATG_G Cuts at: 513 1065 2004 4320 4551 8517 8817 9084 513 Size: 552 939 2316 231 3966 300 267 3550 Fragments arranged by size: 3966 3550 2316 939 552 300 267 231 NdeI CA'TA_TG Cuts at: 387 387 Size: 12121 NgoMIV G'CCGG_C Cuts at: 4872 5053 5180 6545 6576 6872 7456 4872 Size: 181 127 1365 31 296 584 9537 Fragments arranged by size: 9537 1365 584 296 181 127 31 NheI G'CTAG_C Cuts at: 1052 3709 6103 1052 Size: 2657 2394 7070 Fragments arranged by size: 7070 2657 2394 NotI GC'GGCC_GC Cuts at: 9568 9568 Size: 12121 NspI r_CATG'y Cuts at: 1132 1229 2555 3068 4166 5263 6806 8911 8917 Size: 97 1326 513 1098 1097 1543 2105 6 Cuts at: 8917 9479 11024 1132 Size: 562 1545 2229 Fragments arranged by size: 2229 2105 1545 1543 1326 1098 1097 562 513 97 6 PasI CC'CwG_GG Cuts at: 1348 5544 9067 1348 Size: 4196 3523 4402 Fragments arranged by size: 4402 4196 3523 PciI A'CATG_T Cuts at: 11020 11020 Size: 12121 PflMI CCAn_nnn'nTGG Cuts at: 1108 2455 2545 5689 5745 8209 1108 Size: 1347 90 3144 56 2464 5020 Fragments arranged by size: 5020 3144 2464 1347 90 56 PfoI T'CCnGG_A Cuts at: 2049 4374 4715 5109 6048 6492 7314 7337 7473 Size: 2325 341 394 939 444 822 23 136 Cuts at: 7473 7973 8835 9126 2049 Size: 500 862 291 5044 Fragments arranged by size: 5044 2325 939 862 822 500 444 394 341 291 136 23 PmeI GTTT'AAAC Cuts at: 9425 9425 Size: 12121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10190 10222 11703 11735 10190 Size: 32 1481 32 10576 Fragments arranged by size: 10576 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 4657 5419 5845 6052 7150 7387 8275 8697 Size: 2589 762 426 207 1098 237 888 422 Cuts at: 8697 8721 8803 2068 Size: 24 82 5386 Fragments arranged by size: 5386 2589 1098 888 762 426 422 237 207 82 24 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 12121 PsiI TTA'TAA Cuts at: 2220 3807 9691 2220 Size: 1587 5884 4650 Fragments arranged by size: 5884 4650 1587 PspOMI G'GGCC_C Cuts at: 3702 9246 3702 Size: 5544 6577 PstI C_TGCA'G Cuts at: 830 2445 2742 3265 3414 3732 4579 4677 5937 Size: 1615 297 523 149 318 847 98 1260 Cuts at: 5937 6030 6348 6519 7900 8082 8586 9473 830 Size: 93 318 171 1381 182 504 887 3478 Fragments arranged by size: 3478 1615 1381 1260 887 847 523 504 318 318 297 182 171 149 98 93 PvuI CG_AT'CG Cuts at: 2001 5038 2001 Size: 3037 9084 PvuII CAG'CTG Cuts at: 3129 5665 6217 6349 7576 7583 8319 8452 8893 Size: 2536 552 132 1227 7 736 133 441 Cuts at: 8893 8902 3129 Size: 9 6348 Fragments arranged by size: 6348 2536 1227 736 552 441 133 132 9 7 SacI G_AGCT'C Cuts at: 3759 3867 9291 9372 3759 Size: 108 5424 81 6508 Fragments arranged by size: 6508 5424 108 81 SalI G'TCGA_C Cuts at: 1936 9463 1936 Size: 7527 4594 SanDI GG'GwC_CC Cuts at: 8721 8721 Size: 12121 SapI GCTCTTCn'nnn_ Cuts at: 2160 2523 3422 4376 2160 Size: 363 899 954 9905 Fragments arranged by size: 9905 954 899 363 SbfI CC_TGCA'GG Cuts at: 5937 9473 5937 Size: 3536 8585 ScaI AGT'ACT Cuts at: 2465 4688 10369 12081 2465 Size: 2223 5681 1712 2505 Fragments arranged by size: 5681 2505 2223 1712 SexAI A'CCwGG_T Cuts at: 8507 8507 Size: 12121 SfcI C'TryA_G Cuts at: 826 1045 2441 2738 3261 3410 3728 4575 4673 Size: 219 1396 297 523 149 318 847 98 Cuts at: 4673 5312 5657 5933 6026 6344 6515 7896 8078 Size: 639 345 276 93 318 171 1381 182 Cuts at: 8078 8549 8582 9469 10604 11285 11476 826 Size: 471 33 887 1135 681 191 1471 Fragments arranged by size: 1471 1396 1381 1135 887 847 681 639 523 471 345 318 318 297 276 219 191 182 171 149 98 93 33 SfiI GGCCn_nnn'nGGCC Cuts at: 8168 8168 Size: 12121 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12121 SmaI CCC'GGG Cuts at: 1872 5099 6395 7589 9308 9448 1872 Size: 3227 1296 1194 1719 140 4545 Fragments arranged by size: 4545 3227 1719 1296 1194 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 3521 3758 4310 10225 11126 11388 Size: 197 926 1578 237 552 5915 901 262 Cuts at: 11388 820 Size: 1553 Fragments arranged by size: 5915 1578 1553 926 901 552 262 237 197 SnaBI TAC'GTA Cuts at: 493 9912 493 Size: 9419 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 12121 SphI G_CATG'C Cuts at: 1132 6806 8911 8917 9479 1132 Size: 5674 2105 6 562 3774 Fragments arranged by size: 5674 3774 2105 562 6 SspI AAT'ATT Cuts at: 5 52 10045 5 Size: 47 9993 2081 Fragments arranged by size: 9993 2081 47 StuI AGG'CCT Cuts at: 1517 5963 6302 6401 6521 8084 1517 Size: 4446 339 99 120 1563 5554 Fragments arranged by size: 5554 4446 1563 339 120 99 StyI C'CwwG_G Cuts at: 513 1065 2004 3048 3498 3894 3939 4320 4551 Size: 552 939 1044 450 396 45 381 231 Cuts at: 4551 5878 6108 6558 7611 8157 8370 8517 8817 Size: 1327 230 450 1053 546 213 147 300 Cuts at: 8817 9084 9279 9560 513 Size: 267 195 281 3074 Fragments arranged by size: 3074 1327 1053 1044 939 552 546 450 450 396 381 300 281 267 231 230 213 195 147 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2091 3121 4215 5231 5999 10148 10165 Size: 1255 511 1030 1094 1016 768 4149 17 Cuts at: 10165 10318 10503 10948 325 Size: 153 185 445 1498 Fragments arranged by size: 4149 1498 1255 1094 1030 1016 768 511 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2463 3835 4686 Size: 274 80 33 51 1443 486 1372 851 Cuts at: 4686 5707 8847 10367 12079 96 Size: 1021 3140 1520 1712 138 Fragments arranged by size: 3140 1712 1520 1443 1372 1021 851 486 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8138 8170 9124 9156 12081 12113 8138 Size: 32 954 32 2925 32 8146 Fragments arranged by size: 8146 2925 954 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2933 1316 Size: 1617 10504 XbaI T'CTAG_A Cuts at: 2923 9457 2923 Size: 6534 5587 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2305 3946 8524 2305 Size: 1641 4578 5902 Fragments arranged by size: 5902 4578 1641 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 12121 XmaI C'CCGG_G Cuts at: 1870 5097 6393 7587 9306 9446 1870 Size: 3227 1296 1194 1719 140 4545 Fragments arranged by size: 4545 3227 1719 1296 1194 140 XmnI GAAnn'nnTTC Cuts at: 2193 2583 3516 10250 2193 Size: 390 933 6734 4064 Fragments arranged by size: 6734 4064 933 390 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6608 9929 276 Size: 53 83 186 936 5074 3321 2468 Fragments arranged by size: 5074 3321 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BfrBI BstZ17I FseI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfoI SrfI SwaI