(Circular) (Six-base) MAPSORT of: pFN21AB5463.seq Check: 6009 from: 1 to: 5569 pFN21AB5463 5569 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2101 2101 Size: 5569 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3379 278 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2727 2912 1937 Size: 790 185 4594 Fragments arranged by size: 4594 790 185 Acc65I G'GTAC_C Cuts at: 2890 2890 Size: 5569 AclI AA'CG_TT Cuts at: 1563 3696 4069 1563 Size: 2133 373 3063 Fragments arranged by size: 3063 2133 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3632 5016 5284 1632 Size: 2000 1384 268 1917 Fragments arranged by size: 2000 1917 1384 268 AfeI AGC'GCT Cuts at: 2435 2435 Size: 5569 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5372 AflIII A'CryG_T Cuts at: 1203 2646 4468 5445 1203 Size: 1443 1822 977 1327 Fragments arranged by size: 1822 1443 1327 977 AgeI A'CCGG_T Cuts at: 4400 4400 Size: 5569 AhdI GACnn_n'nnGTC Cuts at: 4298 4298 Size: 5569 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5569 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5569 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2315 2347 2315 Size: 32 5537 AlwNI CAG_nnn'CTG Cuts at: 2578 3312 4884 2578 Size: 734 1572 3263 Fragments arranged by size: 3263 1572 734 ApaLI G'TGCA_C Cuts at: 3626 4782 3626 Size: 1156 4413 ApoI r'AATT_y Cuts at: 1493 2878 3104 3256 5365 1493 Size: 1385 226 152 2109 1697 Fragments arranged by size: 2109 1697 1385 226 152 AscI GG'CGCG_CC Cuts at: 2786 2786 Size: 5569 AseI AT'TA_AT Cuts at: 160 1032 4123 160 Size: 872 3091 1606 Fragments arranged by size: 3091 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 AvaI C'yCGr_G Cuts at: 1870 1943 2894 1870 Size: 73 951 4545 Fragments arranged by size: 4545 951 73 BamHI G'GATC_C Cuts at: 2346 2899 3266 2346 Size: 553 367 4649 Fragments arranged by size: 4649 553 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2890 4345 618 Size: 325 217 627 1103 1455 1842 Fragments arranged by size: 1842 1455 1103 627 325 217 BanII G_rGCy'C Cuts at: 1391 2028 2888 1391 Size: 637 860 4072 Fragments arranged by size: 4072 860 637 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2219 2261 2360 928 Size: 971 320 42 99 4137 Fragments arranged by size: 4137 971 320 99 42 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2821 2855 3760 3794 1903 Size: 34 884 34 905 34 3678 Fragments arranged by size: 3678 905 884 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3461 4671 3461 Size: 1210 4359 BclI T'GATC_A Cuts at: 1548 2805 1548 Size: 1257 4312 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2189 4180 136 Size: 107 122 71 631 605 517 1991 1525 Fragments arranged by size: 1991 1525 631 605 517 122 107 71 BglII A'GATC_T Cuts at: 5564 5564 Size: 5569 BlpI GC'TnA_GC Cuts at: 2986 2986 Size: 5569 Bme1580I G_kGCm'C Cuts at: 1790 3630 4786 1790 Size: 1840 1156 2573 Fragments arranged by size: 2573 1840 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5569 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3300 4258 442 Size: 416 53 83 399 24 1883 958 1753 Fragments arranged by size: 1883 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5569 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2483 2519 2531 2542 2639 4229 1239 Size: 1244 36 12 11 97 1590 2579 Fragments arranged by size: 2579 1590 1244 97 36 12 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3694 4559 4857 3694 Size: 865 298 4406 Fragments arranged by size: 4406 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2076 4232 882 Size: 617 577 2156 2219 Fragments arranged by size: 2219 2156 617 577 BsaAI yAC'GTr Cuts at: 493 1204 3360 493 Size: 711 2156 2702 Fragments arranged by size: 2702 2156 711 BsaBI GATnn'nnATC Cuts at: 2810 5563 2810 Size: 2753 2816 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3376 3758 4393 275 Size: 53 83 186 936 1843 382 635 1451 Fragments arranged by size: 1843 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2349 4002 4400 4674 4821 5232 2349 Size: 1653 398 274 147 411 2686 Fragments arranged by size: 2686 1653 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 2381 2411 1178 Size: 30 165 30 912 30 36 30 4336 Fragments arranged by size: 4336 912 165 36 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2174 2237 2320 2507 1186 Size: 946 42 63 83 187 4248 Fragments arranged by size: 4248 946 187 83 63 42 BseYI C'CCAG_C Cuts at: 4772 5268 4772 Size: 496 5073 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4996 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3019 3780 4808 1540 Size: 275 186 1018 761 1028 2301 Fragments arranged by size: 2301 1028 1018 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2028 3630 3715 4786 2028 Size: 1602 85 1071 2811 Fragments arranged by size: 2811 1602 1071 85 BsmI GAATG_Cn' Cuts at: 3080 3173 3080 Size: 93 5476 BsmBI CGTCTCn'nnnn_ Cuts at: 2585 2585 Size: 5569 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2888 3630 3715 4786 1391 Size: 399 238 860 742 85 1071 2174 Fragments arranged by size: 2174 1071 860 742 399 238 85 BspEI T'CCGG_A Cuts at: 2349 5232 2349 Size: 2883 2686 BspHI T'CATG_A Cuts at: 2802 3458 5188 2802 Size: 656 1730 3183 Fragments arranged by size: 3183 1730 656 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2101 2924 844 Size: 1257 823 3489 Fragments arranged by size: 3489 1257 823 BsrBI CCG'CTC Cuts at: 1394 1823 2328 3456 1394 Size: 429 505 1128 3507 Fragments arranged by size: 3507 1128 505 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4064 4238 65 Size: 1183 2816 174 1396 Fragments arranged by size: 2816 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2471 4213 4400 1700 Size: 110 661 1742 187 2869 Fragments arranged by size: 2869 1742 661 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5569 BssHII G'CGCG_C Cuts at: 1925 2786 1925 Size: 861 4708 BssSI C'ACGA_G Cuts at: 2669 3629 4641 2669 Size: 960 1012 3597 Fragments arranged by size: 3597 1012 960 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5569 BstBI TT'CG_AA Cuts at: 4373 4373 Size: 5569 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5569 BstXI CCAn_nnnn'nTGG Cuts at: 2671 2671 Size: 5569 BstYI r'GATC_y Cuts at: 1972 2346 2899 3266 3651 3668 5120 5206 5218 Size: 374 553 367 385 17 1452 86 12 Cuts at: 5218 5564 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 553 385 374 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2342 2342 Size: 5569 BtgI C'CryG_G Cuts at: 513 1065 2073 513 Size: 552 1008 4009 Fragments arranged by size: 4009 1008 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2061 2824 487 Size: 683 56 835 763 3232 Fragments arranged by size: 3232 835 763 683 56 BtsI GCAGTG_nn' Cuts at: 3085 3879 3899 3085 Size: 794 20 4755 Fragments arranged by size: 4755 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5569 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5534 DraI TTT'AAA Cuts at: 2873 2873 Size: 5569 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4576 809 Size: 507 3260 1802 Fragments arranged by size: 3260 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2661 3016 Size: 54 1425 50 275 18 204 627 355 Cuts at: 3016 3905 5534 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 627 355 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3016 1537 Size: 275 1204 4090 Fragments arranged by size: 4090 1204 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3499 1353 Size: 2146 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4156 4530 4676 387 Size: 1064 138 2567 374 146 1280 Fragments arranged by size: 2567 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2026 2026 Size: 5569 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2483 2519 2531 2542 2639 3632 4229 Size: 393 851 36 12 11 97 993 597 Cuts at: 4229 5016 5284 1239 Size: 787 268 1524 Fragments arranged by size: 1524 993 851 787 597 393 268 97 36 12 11 EcoRI G'AATT_C Cuts at: 2878 3256 2878 Size: 378 5191 FspI TGC'GCA Cuts at: 4075 4075 Size: 5569 HaeII r_GCGC'y Cuts at: 1678 2437 4716 1678 Size: 759 2279 2531 Fragments arranged by size: 2531 2279 759 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 4210 4242 4284 Size: 32 135 32 809 32 1797 32 42 Cuts at: 4284 4316 1373 Size: 32 2626 Fragments arranged by size: 2626 1797 809 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2913 3159 669 Size: 988 281 975 246 3079 Fragments arranged by size: 3079 988 975 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5569 HpaI GTT'AAC Cuts at: 3159 3159 Size: 5569 KpnI G_GTAC'C Cuts at: 2894 2894 Size: 5569 MfeI C'AATT_G Cuts at: 3168 3168 Size: 5569 MluI A'CGCG_T Cuts at: 5445 5445 Size: 5569 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2268 2379 2445 4683 4867 1305 Size: 963 111 66 2238 184 2007 Fragments arranged by size: 2238 2007 963 184 111 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 2663 10 Size: 54 1425 343 831 2916 Fragments arranged by size: 2916 1425 831 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2651 2657 2663 2669 3527 3886 4045 Size: 648 1485 6 6 6 858 359 159 Cuts at: 4045 518 Size: 2042 Fragments arranged by size: 2042 1485 858 648 359 159 6 6 6 MspA1I CmG'CkG Cuts at: 1606 2336 2461 2985 3662 4810 5055 1606 Size: 730 125 524 677 1148 245 2120 Fragments arranged by size: 2120 1148 730 677 524 245 125 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5017 NdeI CA'TA_TG Cuts at: 387 387 Size: 5569 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5569 NotI GC'GGCC_GC Cuts at: 3016 3016 Size: 5569 NspI r_CATG'y Cuts at: 1132 1229 2509 2650 2927 4472 1132 Size: 97 1280 141 277 1545 2229 Fragments arranged by size: 2229 1545 1280 277 141 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5569 PciI A'CATG_T Cuts at: 2646 4468 2646 Size: 1822 3747 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5569 PmeI GTTT'AAAC Cuts at: 2873 2873 Size: 5569 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2315 2347 3638 3670 5151 5183 2315 Size: 32 1291 32 1481 32 2701 Fragments arranged by size: 2701 1481 1291 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5569 PsiI TTA'TAA Cuts at: 3139 3139 Size: 5569 PstI C_TGCA'G Cuts at: 830 2921 830 Size: 2091 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5569 PvuII CAG'CTG Cuts at: 2461 2461 Size: 5569 SacI G_AGCT'C Cuts at: 2028 2028 Size: 5569 SalI G'TCGA_C Cuts at: 1936 2911 1936 Size: 975 4594 SbfI CC_TGCA'GG Cuts at: 2921 2921 Size: 5569 ScaI AGT'ACT Cuts at: 3817 5529 3817 Size: 1712 3857 SfcI C'TryA_G Cuts at: 826 1045 2917 4052 4733 4924 826 Size: 219 1872 1135 681 191 1471 Fragments arranged by size: 1872 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5569 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5569 SmaI CCC'GGG Cuts at: 1872 2896 1872 Size: 1024 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3673 4574 4836 820 Size: 197 926 1730 901 262 1553 Fragments arranged by size: 1730 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3360 493 Size: 2867 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5569 SphI G_CATG'C Cuts at: 1132 2927 1132 Size: 1795 3774 SspI AAT'ATT Cuts at: 5 52 3493 5 Size: 47 3441 2081 Fragments arranged by size: 3441 2081 47 StuI AGG'CCT Cuts at: 1517 2319 1517 Size: 802 4767 StyI C'CwwG_G Cuts at: 513 1065 2037 3008 513 Size: 552 972 971 3074 Fragments arranged by size: 3074 972 971 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2028 2094 3596 3613 3766 3951 4396 Size: 1255 448 66 1502 17 153 185 445 Cuts at: 4396 325 Size: 1498 Fragments arranged by size: 1502 1498 1255 448 445 185 153 66 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2403 2706 3815 Size: 274 80 33 51 1443 426 303 1109 Cuts at: 3815 5527 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1109 426 303 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2164 2196 5529 5561 2164 Size: 32 3333 32 2172 Fragments arranged by size: 3333 2172 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5569 XbaI T'CTAG_A Cuts at: 2905 2905 Size: 5569 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2383 2494 2383 Size: 111 5458 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5569 XmaI C'CCGG_G Cuts at: 1870 2894 1870 Size: 1024 4545 XmnI GAAnn'nnTTC Cuts at: 3698 3698 Size: 5569 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3377 276 Size: 53 83 186 936 1843 2468 Fragments arranged by size: 2468 1843 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI