(Circular) (Six-base) MAPSORT of: pFN21AB5406.seq Check: 8469 from: 1 to: 5698 pFN21AB5406 5698 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2161 2161 Size: 5698 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2090 3041 1937 Size: 153 951 4594 Fragments arranged by size: 4594 951 153 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2007 2543 3761 5145 5413 1632 Size: 375 536 1218 1384 268 1917 Fragments arranged by size: 1917 1384 1218 536 375 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 4597 5574 1203 Size: 3394 977 1327 Fragments arranged by size: 3394 1327 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AlwNI CAG_nnn'CTG Cuts at: 2968 3441 5013 2968 Size: 473 1572 3653 Fragments arranged by size: 3653 1572 473 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 3007 3233 3385 5494 1493 Size: 1514 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 226 152 AseI AT'TA_AT Cuts at: 160 1032 2438 4252 160 Size: 872 1406 1814 1606 Fragments arranged by size: 1814 1606 1406 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 3023 1870 Size: 73 1080 4545 Fragments arranged by size: 4545 1080 73 BamHI G'GATC_C Cuts at: 3028 3395 3028 Size: 367 5331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2600 3019 4474 618 Size: 325 217 627 813 419 1455 1842 Fragments arranged by size: 1842 1455 813 627 419 325 217 BanII G_rGCy'C Cuts at: 1391 3017 1391 Size: 1626 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4727 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4309 136 Size: 107 122 71 631 605 2637 1525 Fragments arranged by size: 2637 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2319 5693 2319 Size: 3374 2324 BlpI GC'TnA_GC Cuts at: 3115 3115 Size: 5698 Bme1580I G_kGCm'C Cuts at: 1790 3759 4915 1790 Size: 1969 1156 2573 Fragments arranged by size: 2573 1969 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5698 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3429 4387 442 Size: 416 53 83 399 24 2012 958 1753 Fragments arranged by size: 2012 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2354 2879 4358 1239 Size: 1115 525 1479 2579 Fragments arranged by size: 2579 1479 1115 525 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2043 2819 3823 4688 4986 2043 Size: 776 1004 865 298 2755 Fragments arranged by size: 2755 1004 865 776 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2057 2855 4361 882 Size: 617 558 798 1506 2219 Fragments arranged by size: 2219 1506 798 617 558 BsaAI yAC'GTr Cuts at: 493 1204 3489 493 Size: 711 2285 2702 Fragments arranged by size: 2702 2285 711 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3505 3887 4522 275 Size: 53 83 186 936 1972 382 635 1451 Fragments arranged by size: 1972 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4131 4529 4803 4950 5361 4131 Size: 398 274 147 411 4468 Fragments arranged by size: 4468 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2711 2741 1178 Size: 30 165 30 1308 30 4135 Fragments arranged by size: 4135 1308 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5698 BseYI C'CCAG_C Cuts at: 2035 4901 5397 2035 Size: 2866 496 2336 Fragments arranged by size: 2866 2336 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2273 1163 Size: 573 537 4588 Fragments arranged by size: 4588 573 537 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3148 3909 4937 1540 Size: 275 186 1147 761 1028 2301 Fragments arranged by size: 2301 1147 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2976 3759 3844 4915 2976 Size: 783 85 1071 3759 Fragments arranged by size: 3759 1071 783 85 BsmI GAATG_Cn' Cuts at: 2517 3209 3302 2517 Size: 692 93 4913 Fragments arranged by size: 4913 692 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 2003 Size: 5698 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2976 3017 3759 3844 4915 1391 Size: 399 1186 41 742 85 1071 2174 Fragments arranged by size: 2174 1186 1071 742 399 85 41 BspEI T'CCGG_A Cuts at: 5361 5361 Size: 5698 BspHI T'CATG_A Cuts at: 2100 3587 5317 2100 Size: 1487 1730 2481 Fragments arranged by size: 2481 1730 1487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2161 2853 3053 844 Size: 1317 692 200 3489 Fragments arranged by size: 3489 1317 692 200 BsrBI CCG'CTC Cuts at: 1394 1823 3585 1394 Size: 429 1762 3507 Fragments arranged by size: 3507 1762 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4193 4367 65 Size: 1183 2945 174 1396 Fragments arranged by size: 2945 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4342 4529 1700 Size: 110 2532 187 2869 Fragments arranged by size: 2869 2532 187 110 BsrGI T'GTAC_A Cuts at: 96 2496 2665 96 Size: 2400 169 3129 Fragments arranged by size: 3129 2400 169 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5698 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5698 BstBI TT'CG_AA Cuts at: 4502 4502 Size: 5698 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5698 BstXI CCAn_nnnn'nTGG Cuts at: 2581 2581 Size: 5698 BstYI r'GATC_y Cuts at: 1972 2319 2451 3028 3395 3780 3797 5249 5335 Size: 347 132 577 367 385 17 1452 86 Cuts at: 5335 5347 5693 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 577 385 367 347 346 132 86 17 12 BstZ17I GTA'TAC Cuts at: 2091 2091 Size: 5698 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4207 Fragments arranged by size: 4207 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2096 487 Size: 683 56 870 4089 Fragments arranged by size: 4089 870 683 56 BtsI GCAGTG_nn' Cuts at: 3214 4008 4028 3214 Size: 794 20 4884 Fragments arranged by size: 4884 794 20 ClaI AT'CG_AT Cuts at: 1554 2809 1554 Size: 1255 4443 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 2383 3002 2383 Size: 619 5079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3145 4034 5663 Size: 54 1425 50 275 18 1315 889 1629 Cuts at: 5663 8 Size: 43 Fragments arranged by size: 1629 1425 1315 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3145 1537 Size: 275 1333 4090 Fragments arranged by size: 4090 1333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2437 2580 3628 1353 Size: 1084 143 1048 3423 Fragments arranged by size: 3423 1084 1048 143 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4285 4659 4805 387 Size: 1064 138 2696 374 146 1280 Fragments arranged by size: 2696 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2354 2543 2879 3761 4358 5145 Size: 393 375 347 189 336 882 597 787 Cuts at: 5145 5413 1239 Size: 268 1524 Fragments arranged by size: 1524 882 787 597 393 375 347 336 268 189 EcoO109I rG'GnC_Cy Cuts at: 2965 2965 Size: 5698 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 4845 1678 Size: 3167 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2414 2446 2465 2497 2555 Size: 32 135 32 842 32 19 32 58 Cuts at: 2555 2587 2711 2743 4339 4371 4413 4445 1373 Size: 32 124 32 1596 32 42 32 2626 Fragments arranged by size: 2626 1596 842 135 124 58 42 32 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 3042 3288 669 Size: 988 281 1104 246 3079 Fragments arranged by size: 3079 1104 988 281 246 HindIII A'AGCT_T Cuts at: 748 2181 748 Size: 1433 4265 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2100 4812 4996 1305 Size: 795 2712 184 2007 Fragments arranged by size: 2712 2007 795 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3876 Fragments arranged by size: 3876 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3656 4015 4174 518 Size: 648 2490 359 159 2042 Fragments arranged by size: 2490 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 3114 3791 4939 5184 1606 Size: 433 1075 677 1148 245 2120 Fragments arranged by size: 2120 1148 1075 677 433 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4207 Fragments arranged by size: 4207 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 2260 3056 4601 1132 Size: 97 1031 796 1545 2229 Fragments arranged by size: 2229 1545 1031 796 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5698 PciI A'CATG_T Cuts at: 4597 4597 Size: 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5698 PfoI T'CCnGG_A Cuts at: 2370 2370 Size: 5698 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PstI C_TGCA'G Cuts at: 830 2172 2964 3050 830 Size: 1342 792 86 3478 Fragments arranged by size: 3478 1342 792 86 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 PvuII CAG'CTG Cuts at: 2039 2039 Size: 5698 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SapI GCTCTTCn'nnn_ Cuts at: 2580 2580 Size: 5698 SbfI CC_TGCA'GG Cuts at: 2172 2964 3050 2172 Size: 792 86 4820 Fragments arranged by size: 4820 792 86 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SfcI C'TryA_G Cuts at: 826 1045 2168 2591 2960 3046 4181 4862 5053 Size: 219 1123 423 369 86 1135 681 191 Cuts at: 5053 826 Size: 1471 Fragments arranged by size: 1471 1135 1123 681 423 369 219 191 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2058 2834 3802 4703 4965 820 Size: 197 926 115 776 968 901 262 1553 Fragments arranged by size: 1553 968 926 901 776 262 197 115 SnaBI TAC'GTA Cuts at: 493 3489 493 Size: 2996 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 2260 3056 1132 Size: 1128 796 3774 Fragments arranged by size: 3774 1128 796 SspI AAT'ATT Cuts at: 5 52 2614 3622 5 Size: 47 2562 1008 2081 Fragments arranged by size: 2562 2081 1008 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5698 StyI C'CwwG_G Cuts at: 513 1065 2004 2310 3137 513 Size: 552 939 306 827 3074 Fragments arranged by size: 3074 939 827 552 306 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2522 3725 3742 3895 4080 4525 325 Size: 1255 942 1203 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1203 942 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2496 2665 3944 Size: 274 80 33 51 1443 519 169 1279 Cuts at: 3944 5656 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1279 519 274 169 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5658 5690 5658 Size: 32 5666 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5698 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2617 2617 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 3827 3827 Size: 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI