(Circular) (Six-base) MAPSORT of: pFN21AB5400.seq Check: 1734 from: 1 to: 6118 pFN21AB5400 6118 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3928 278 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3461 1937 Size: 1524 4594 Acc65I G'GTAC_C Cuts at: 3230 3439 3230 Size: 209 5909 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2201 4181 5565 5833 1632 Size: 569 1980 1384 268 1917 Fragments arranged by size: 1980 1917 1384 569 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 5017 5994 1203 Size: 3814 977 1327 Fragments arranged by size: 3814 1327 977 AgeI A'CCGG_T Cuts at: 4949 4949 Size: 6118 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6118 AlwNI CAG_nnn'CTG Cuts at: 3251 3380 3861 5433 3251 Size: 129 481 1572 3936 Fragments arranged by size: 3936 1572 481 129 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 2185 3389 3427 3653 3805 5914 1493 Size: 692 1204 38 226 152 2109 1697 Fragments arranged by size: 2109 1697 1204 692 226 152 38 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 3443 1870 Size: 73 1500 4545 Fragments arranged by size: 4545 1500 73 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2958 3051 3200 3230 3439 Size: 325 217 627 1171 93 149 30 209 Cuts at: 3439 4894 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1171 627 325 217 209 149 93 30 BanII G_rGCy'C Cuts at: 1391 2682 3030 3437 1391 Size: 1291 348 407 4072 Fragments arranged by size: 4072 1291 407 348 BbeI G_GCGC'C Cuts at: 2962 3055 2962 Size: 93 6025 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2213 2305 928 Size: 971 314 92 4741 Fragments arranged by size: 4741 971 314 92 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3131 3165 4309 4343 1903 Size: 34 50 34 1110 34 1144 34 3678 Fragments arranged by size: 3678 1144 1110 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4010 5220 4010 Size: 1210 4908 BclI T'GATC_A Cuts at: 1548 1548 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4729 136 Size: 107 122 71 631 605 3057 1525 Fragments arranged by size: 3057 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6113 6113 Size: 6118 BlpI GC'TnA_GC Cuts at: 3535 3535 Size: 6118 Bme1580I G_kGCm'C Cuts at: 1790 4179 5335 1790 Size: 2389 1156 2573 Fragments arranged by size: 2573 2389 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6118 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3849 4807 442 Size: 416 53 83 399 24 2432 958 1753 Fragments arranged by size: 2432 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2912 3044 3228 3402 4778 1239 Size: 1673 132 184 174 1376 2579 Fragments arranged by size: 2579 1673 1376 184 174 132 Bpu10I CC'TnA_GC Cuts at: 3029 3029 Size: 6118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4243 5108 5406 4243 Size: 865 298 4955 Fragments arranged by size: 4955 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4781 882 Size: 617 3282 2219 Fragments arranged by size: 3282 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3909 493 Size: 711 2705 2702 Fragments arranged by size: 2705 2702 711 BsaBI GATnn'nnATC Cuts at: 6112 6112 Size: 6118 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2959 3052 3925 4307 Size: 53 83 186 936 1426 93 873 382 Cuts at: 4307 4942 275 Size: 635 1451 Fragments arranged by size: 1451 1426 936 873 635 382 186 93 83 53 BsaWI w'CCGG_w Cuts at: 4551 4949 5223 5370 5781 4551 Size: 398 274 147 411 4888 Fragments arranged by size: 4888 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3223 3253 1178 Size: 30 165 30 1820 30 4043 Fragments arranged by size: 4043 1820 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3231 3345 1186 Size: 2045 114 3959 Fragments arranged by size: 3959 2045 114 BseYI C'CCAG_C Cuts at: 3235 5321 5817 3235 Size: 2086 496 3536 Fragments arranged by size: 3536 2086 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2895 2975 3393 1163 Size: 573 1159 80 418 3888 Fragments arranged by size: 3888 1159 573 418 80 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3568 4329 5357 1540 Size: 275 186 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2925 3030 4179 4264 5335 2925 Size: 105 1149 85 1071 3708 Fragments arranged by size: 3708 1149 1071 105 85 BsmI GAATG_Cn' Cuts at: 3362 3629 3722 3362 Size: 267 93 5758 Fragments arranged by size: 5758 267 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2682 2925 3030 3437 4179 4264 5335 Size: 399 892 243 105 407 742 85 1071 Cuts at: 5335 1391 Size: 2174 Fragments arranged by size: 2174 1071 892 742 407 399 243 105 85 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 4007 5737 4007 Size: 1730 4388 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2137 3259 3473 844 Size: 1293 1122 214 3489 Fragments arranged by size: 3489 1293 1122 214 BsrBI CCG'CTC Cuts at: 1394 1823 4005 1394 Size: 429 2182 3507 Fragments arranged by size: 3507 2182 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4613 4787 65 Size: 1183 3365 174 1396 Fragments arranged by size: 3365 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4762 4949 1700 Size: 110 2952 187 2869 Fragments arranged by size: 2952 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 2880 4178 5190 2880 Size: 1298 1012 3808 Fragments arranged by size: 3808 1298 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6118 BstBI TT'CG_AA Cuts at: 2461 4922 2461 Size: 2461 3657 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstYI r'GATC_y Cuts at: 1972 3448 3815 4200 4217 5669 5755 5767 6113 Size: 1476 367 385 17 1452 86 12 346 Cuts at: 6113 1972 Size: 1977 Fragments arranged by size: 1977 1476 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2566 2566 Size: 6118 BtgI C'CryG_G Cuts at: 513 1065 2407 2682 513 Size: 552 1342 275 3949 Fragments arranged by size: 3949 1342 552 275 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 2635 3634 4428 4448 2635 Size: 999 794 20 4305 Fragments arranged by size: 4305 999 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 3422 3422 Size: 6118 DraIII CAC_nnn'GTG Cuts at: 2408 2408 Size: 6118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5125 809 Size: 507 3809 1802 Fragments arranged by size: 3809 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2518 3565 4454 Size: 54 1425 50 275 18 688 1047 889 Cuts at: 4454 6083 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1047 889 688 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2697 2979 3036 3057 4048 1353 Size: 1344 282 57 21 991 3423 Fragments arranged by size: 3423 1344 991 282 57 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4705 5079 5225 387 Size: 1064 138 3116 374 146 1280 Fragments arranged by size: 3116 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3028 3028 Size: 6118 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2912 3044 3228 3402 4181 4778 Size: 393 569 711 132 184 174 779 597 Cuts at: 4778 5565 5833 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 779 711 597 569 393 268 184 174 132 EcoO109I rG'GnC_Cy Cuts at: 2562 3248 2562 Size: 686 5432 EcoRI G'AATT_C Cuts at: 3427 3805 3427 Size: 378 5740 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2726 2987 3139 2726 Size: 261 152 5705 Fragments arranged by size: 5705 261 152 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 2962 3055 5265 1678 Size: 1284 93 2210 2531 Fragments arranged by size: 2531 2210 1284 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4759 4791 4833 4865 1373 Size: 32 135 32 3187 32 42 32 2626 Fragments arranged by size: 3187 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3462 3708 669 Size: 988 281 1524 246 3079 Fragments arranged by size: 3079 1524 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6118 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KasI G'GCGC_C Cuts at: 2958 3051 2958 Size: 93 6025 KpnI G_GTAC'C Cuts at: 3234 3443 3234 Size: 209 5909 MfeI C'AATT_G Cuts at: 3342 3717 3342 Size: 375 5743 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2508 5232 5416 1305 Size: 1203 2724 184 2007 Fragments arranged by size: 2724 2007 1203 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2520 10 Size: 54 1425 343 688 3608 Fragments arranged by size: 3608 1425 688 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2831 2936 3260 4076 4435 4594 518 Size: 648 1665 105 324 816 359 159 2042 Fragments arranged by size: 2042 1665 816 648 359 324 159 105 MspA1I CmG'CkG Cuts at: 1606 2419 2603 3227 3534 4211 5359 5604 1606 Size: 813 184 624 307 677 1148 245 2120 Fragments arranged by size: 2120 1148 813 677 624 307 245 184 NarI GG'CG_CC Cuts at: 2959 3052 2959 Size: 93 6025 NcoI C'CATG_G Cuts at: 513 1065 2682 513 Size: 552 1617 3949 Fragments arranged by size: 3949 1617 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 3476 5021 1132 Size: 97 2247 1545 2229 Fragments arranged by size: 2247 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6118 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 2408 2814 1108 Size: 1300 406 4412 Fragments arranged by size: 4412 1300 406 PfoI T'CCnGG_A Cuts at: 3244 3379 3244 Size: 135 5983 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3248 3248 Size: 6118 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6118 PsiI TTA'TAA Cuts at: 2729 3688 2729 Size: 959 5159 PstI C_TGCA'G Cuts at: 830 3470 830 Size: 2640 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 PvuII CAG'CTG Cuts at: 2419 2603 3227 2419 Size: 184 624 5310 Fragments arranged by size: 5310 624 184 SacI G_AGCT'C Cuts at: 3030 3030 Size: 6118 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SapI GCTCTTCn'nnn_ Cuts at: 2979 3036 2979 Size: 57 6061 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 4366 6078 4366 Size: 1712 4406 SexAI A'CCwGG_T Cuts at: 2925 2925 Size: 6118 SfcI C'TryA_G Cuts at: 826 1045 2535 3466 4601 5282 5473 826 Size: 219 1490 931 1135 681 191 1471 Fragments arranged by size: 1490 1471 1135 931 681 219 191 SfoI GGC'GCC Cuts at: 2960 3053 2960 Size: 93 6025 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6118 SmaI CCC'GGG Cuts at: 1872 3445 1872 Size: 1573 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4222 5123 5385 820 Size: 197 926 2279 901 262 1553 Fragments arranged by size: 2279 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 3476 1132 Size: 2344 3774 SspI AAT'ATT Cuts at: 5 52 4042 5 Size: 47 3990 2081 Fragments arranged by size: 3990 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6118 StyI C'CwwG_G Cuts at: 513 1065 2682 3557 513 Size: 552 1617 875 3074 Fragments arranged by size: 3074 1617 875 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2231 2782 4145 4162 4315 4500 4945 Size: 1255 651 551 1363 17 153 185 445 Cuts at: 4945 325 Size: 1498 Fragments arranged by size: 1498 1363 1255 651 551 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4364 6076 96 Size: 274 80 33 51 1443 2387 1712 138 Fragments arranged by size: 2387 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6078 6110 6078 Size: 32 6086 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6118 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2386 2491 2386 Size: 105 6013 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6118 XmaI C'CCGG_G Cuts at: 1870 3443 1870 Size: 1573 4545 XmnI GAAnn'nnTTC Cuts at: 2210 2970 4247 2210 Size: 760 1277 4081 Fragments arranged by size: 4081 1277 760 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3926 276 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI