Restriction map for
pFN21AB5359
(Circular) (Six-base) MAPSORT of: pFN21AB5359.seq Check: 8053 from: 1 to: 5254
pFN21AB5359 5254 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3064 278
Size: 53 83 186 936 1528 2468
Fragments arranged by size:
2468 1528 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2597 1937
Size: 660 4594
Acc65I G'GTAC_C
Cuts at: 2575 2575
Size: 5254
AclI AA'CG_TT
Cuts at: 1563 3381 3754 1563
Size: 1818 373 3063
Fragments arranged by size:
3063 1818 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2106 2171 2214 3317 4701 4969 1632
Size: 474 65 43 1103 1384 268 1917
Fragments arranged by size:
1917 1384 1103 474 268 65 43
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5057
AflIII A'CryG_T
Cuts at: 1203 2119 4153 5130 1203
Size: 916 2034 977 1327
Fragments arranged by size:
2034 1327 977 916
AgeI A'CCGG_T
Cuts at: 4085 4085
Size: 5254
AhdI GACnn_n'nnGTC
Cuts at: 3983 3983
Size: 5254
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5254
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5254
AlwNI CAG_nnn'CTG
Cuts at: 2084 2174 2997 4569 2084
Size: 90 823 1572 2769
Fragments arranged by size:
2769 1572 823 90
ApaLI G'TGCA_C
Cuts at: 3311 4467 3311
Size: 1156 4098
ApoI r'AATT_y
Cuts at: 1493 2090 2200 2563 2789 2941 5050 1493
Size: 597 110 363 226 152 2109 1697
Fragments arranged by size:
2109 1697 597 363 226 152 110
AseI AT'TA_AT
Cuts at: 160 1032 3808 160
Size: 872 2776 1606
Fragments arranged by size:
2776 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5254
AvaI C'yCGr_G
Cuts at: 1870 1943 2579 1870
Size: 73 636 4545
Fragments arranged by size:
4545 636 73
BamHI G'GATC_C
Cuts at: 2584 2951 2584
Size: 367 4887
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2387 2575 4030 618
Size: 325 217 627 600 188 1455 1842
Fragments arranged by size:
1842 1455 627 600 325 217 188
BanII G_rGCy'C
Cuts at: 1391 2573 1391
Size: 1182 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4283
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3445 3479 1903
Size: 34 1508 34 3678
Fragments arranged by size:
3678 1508 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3146 4356 3146
Size: 1210 4044
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5254
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3865 136
Size: 107 122 71 631 605 2193 1525
Fragments arranged by size:
2193 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5249 5249
Size: 5254
BlpI GC'TnA_GC
Cuts at: 2671 2671
Size: 5254
Bme1580I G_kGCm'C
Cuts at: 1790 3315 4471 1790
Size: 1525 1156 2573
Fragments arranged by size:
2573 1525 1156
BmgBI CAC'GTC
Cuts at: 1318 2120 1318
Size: 802 4452
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2985 3943 442
Size: 416 53 83 399 24 1568 958 1753
Fragments arranged by size:
1753 1568 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3914 1239
Size: 2675 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2535 3379 4244 4542 2535
Size: 844 865 298 3247
Fragments arranged by size:
3247 865 844 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3917 882
Size: 617 2418 2219
Fragments arranged by size:
2418 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3045 493
Size: 711 1841 2702
Fragments arranged by size:
2702 1841 711
BsaBI GATnn'nnATC
Cuts at: 5248 5248
Size: 5254
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3061 3443 4078 275
Size: 53 83 186 936 1528 382 635 1451
Fragments arranged by size:
1528 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3687 4085 4359 4506 4917 3687
Size: 398 274 147 411 4024
Fragments arranged by size:
4024 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5029
Fragments arranged by size:
5029 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5254
BseYI C'CCAG_C
Cuts at: 4457 4953 4457
Size: 496 4758
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4681
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2704 3465 4493 1540
Size: 275 186 703 761 1028 2301
Fragments arranged by size:
2301 1028 761 703 275 186
BsiHKAI G_wGCw'C
Cuts at: 3315 3400 4471 3315
Size: 85 1071 4098
Fragments arranged by size:
4098 1071 85
BsmI GAATG_Cn'
Cuts at: 2527 2765 2858 2527
Size: 238 93 4923
Fragments arranged by size:
4923 238 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2573 3315 3400 4471 1391
Size: 399 783 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 399 85
BspEI T'CCGG_A
Cuts at: 4917 4917
Size: 5254
BspHI T'CATG_A
Cuts at: 2421 3143 4873 2421
Size: 722 1730 2802
Fragments arranged by size:
2802 1730 722
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2609 844
Size: 1765 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3141 1394
Size: 429 1318 3507
Fragments arranged by size:
3507 1318 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2024 3749 3923 65
Size: 1183 776 1725 174 1396
Fragments arranged by size:
1725 1396 1183 776 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3898 4085 1700
Size: 110 2088 187 2869
Fragments arranged by size:
2869 2088 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5254
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5254
BssSI C'ACGA_G
Cuts at: 3314 4326 3314
Size: 1012 4242
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5254
BstBI TT'CG_AA
Cuts at: 4058 4058
Size: 5254
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5254
BstYI r'GATC_y
Cuts at: 1972 2584 2951 3336 3353 4805 4891 4903 5249
Size: 612 367 385 17 1452 86 12 346
Cuts at: 5249 1972
Size: 1977
Fragments arranged by size:
1977 1452 612 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3763
Fragments arranged by size:
3763 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4515
Fragments arranged by size:
4515 683 56
BtsI GCAGTG_nn'
Cuts at: 2407 2770 3564 3584 2407
Size: 363 794 20 4077
Fragments arranged by size:
4077 794 363 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5254
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5219
DraI TTT'AAA
Cuts at: 2558 2558
Size: 5254
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4261 809
Size: 507 2945 1802
Fragments arranged by size:
2945 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2701 3590 5219
Size: 54 1425 50 275 18 871 889 1629
Cuts at: 5219 8
Size: 43
Fragments arranged by size:
1629 1425 889 871 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2701 1537
Size: 275 889 4090
Fragments arranged by size:
4090 889 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 2505 3184 1353
Size: 714 438 679 3423
Fragments arranged by size:
3423 714 679 438
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3841 4215 4361 387
Size: 1064 138 2252 374 146 1280
Fragments arranged by size:
2252 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2171 2214 3317 3914 4701 4969
Size: 393 474 65 43 1103 597 787 268
Cuts at: 4969 1239
Size: 1524
Fragments arranged by size:
1524 1103 787 597 474 393 268 65
43
EcoRI G'AATT_C
Cuts at: 2563 2941 2563
Size: 378 4876
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2249 2298 2249
Size: 49 5205
FspI TGC'GCA
Cuts at: 3760 3760
Size: 5254
HaeII r_GCGC'y
Cuts at: 1678 4401 1678
Size: 2723 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2297 2314 2329 3895
Size: 32 135 32 710 15 17 15 1566
Cuts at: 3895 3927 3969 4001 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1566 710 135 42 32 32 32
32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2598 2844 669
Size: 988 281 660 246 3079
Fragments arranged by size:
3079 988 660 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5254
HpaI GTT'AAC
Cuts at: 2844 2844
Size: 5254
KpnI G_GTAC'C
Cuts at: 2579 2579
Size: 5254
MfeI C'AATT_G
Cuts at: 2853 2853
Size: 5254
MluI A'CGCG_T
Cuts at: 5130 5130
Size: 5254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 4368 4552 1305
Size: 732 2331 184 2007
Fragments arranged by size:
2331 2007 732 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3432
Fragments arranged by size:
3432 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3212 3571 3730 518
Size: 648 2046 359 159 2042
Fragments arranged by size:
2046 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2670 3347 4495 4740 1606
Size: 1064 677 1148 245 2120
Fragments arranged by size:
2120 1148 1064 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3763
Fragments arranged by size:
3763 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5254
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5254
NotI GC'GGCC_GC
Cuts at: 2701 2701
Size: 5254
NspI r_CATG'y
Cuts at: 1132 1229 2612 4157 1132
Size: 97 1383 1545 2229
Fragments arranged by size:
2229 1545 1383 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5254
PciI A'CATG_T
Cuts at: 4153 4153
Size: 5254
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5254
PmeI GTTT'AAAC
Cuts at: 2558 2558
Size: 5254
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 4836 4868 3323
Size: 32 1481 32 3709
Fragments arranged by size:
3709 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5254
PsiI TTA'TAA
Cuts at: 2824 2824
Size: 5254
PstI C_TGCA'G
Cuts at: 830 2606 830
Size: 1776 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5254
SalI G'TCGA_C
Cuts at: 1936 2596 1936
Size: 660 4594
SbfI CC_TGCA'GG
Cuts at: 2606 2606
Size: 5254
ScaI AGT'ACT
Cuts at: 2109 3502 5214 2109
Size: 1393 1712 2149
Fragments arranged by size:
2149 1712 1393
SfcI C'TryA_G
Cuts at: 826 1045 2602 3737 4418 4609 826
Size: 219 1557 1135 681 191 1471
Fragments arranged by size:
1557 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5254
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5254
SmaI CCC'GGG
Cuts at: 1872 2581 1872
Size: 709 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2550 3358 4259 4521 820
Size: 197 926 607 808 901 262 1553
Fragments arranged by size:
1553 926 901 808 607 262 197
SnaBI TAC'GTA
Cuts at: 493 3045 493
Size: 2552 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5254
SphI G_CATG'C
Cuts at: 1132 2612 1132
Size: 1480 3774
SspI AAT'ATT
Cuts at: 5 52 2144 3178 5
Size: 47 2092 1034 2081
Fragments arranged by size:
2092 2081 1034 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5254
StyI C'CwwG_G
Cuts at: 513 1065 2004 2693 513
Size: 552 939 689 3074
Fragments arranged by size:
3074 939 689 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3281 3298 3451 3636 4081 325
Size: 1255 1701 17 153 185 445 1498
Fragments arranged by size:
1701 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2107 3500 5212
Size: 274 80 33 51 1443 130 1393 1712
Cuts at: 5212 96
Size: 138
Fragments arranged by size:
1712 1443 1393 274 138 130 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5214 5246 5214
Size: 32 5222
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5254
XbaI T'CTAG_A
Cuts at: 2590 2590
Size: 5254
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5254
XmaI C'CCGG_G
Cuts at: 1870 2579 1870
Size: 709 4545
XmnI GAAnn'nnTTC
Cuts at: 2229 3383 2229
Size: 1154 4100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3062 276
Size: 53 83 186 936 1528 2468
Fragments arranged by size:
2468 1528 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI