(Circular) (Six-base) MAPSORT of: pFN21AB5332.seq Check: 9400 from: 1 to: 5530 pFN21AB5332 5530 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3340 278 Size: 53 83 186 936 1804 2468 Fragments arranged by size: 2468 1804 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2873 1937 Size: 936 4594 Acc65I G'GTAC_C Cuts at: 2851 2851 Size: 5530 AclI AA'CG_TT Cuts at: 1563 3657 4030 1563 Size: 2094 373 3063 Fragments arranged by size: 3063 2094 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3593 4977 5245 1632 Size: 1961 1384 268 1917 Fragments arranged by size: 1961 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5333 AflIII A'CryG_T Cuts at: 1203 2270 2344 4429 5406 1203 Size: 1067 74 2085 977 1327 Fragments arranged by size: 2085 1327 1067 977 74 AgeI A'CCGG_T Cuts at: 4361 4361 Size: 5530 AhdI GACnn_n'nnGTC Cuts at: 4259 4259 Size: 5530 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5530 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5530 AlwNI CAG_nnn'CTG Cuts at: 2479 3273 4845 2479 Size: 794 1572 3164 Fragments arranged by size: 3164 1572 794 ApaLI G'TGCA_C Cuts at: 3587 4743 3587 Size: 1156 4374 ApoI r'AATT_y Cuts at: 1493 2189 2839 3065 3217 5326 1493 Size: 696 650 226 152 2109 1697 Fragments arranged by size: 2109 1697 696 650 226 152 AseI AT'TA_AT Cuts at: 160 1032 2213 4084 160 Size: 872 1181 1871 1606 Fragments arranged by size: 1871 1606 1181 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 AvaI C'yCGr_G Cuts at: 1870 1943 2259 2288 2355 2379 2603 2855 1870 Size: 73 316 29 67 24 224 252 4545 Fragments arranged by size: 4545 316 252 224 73 67 29 24 BamHI G'GATC_C Cuts at: 2860 3227 2860 Size: 367 5163 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2851 4306 618 Size: 325 217 627 1064 1455 1842 Fragments arranged by size: 1842 1455 1064 627 325 217 BanII G_rGCy'C Cuts at: 1391 2046 2849 1391 Size: 655 803 4072 Fragments arranged by size: 4072 803 655 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2107 2337 928 Size: 971 208 230 4121 Fragments arranged by size: 4121 971 230 208 BbvCI CC'TCA_GC Cuts at: 2124 2124 Size: 5530 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2240 2274 3721 3755 1903 Size: 34 50 34 219 34 1447 34 3678 Fragments arranged by size: 3678 1447 219 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3422 4632 3422 Size: 1210 4320 BclI T'GATC_A Cuts at: 1548 1548 Size: 5530 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4141 136 Size: 107 122 71 631 605 2469 1525 Fragments arranged by size: 2469 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5525 5525 Size: 5530 BlpI GC'TnA_GC Cuts at: 2947 2947 Size: 5530 Bme1580I G_kGCm'C Cuts at: 1790 3591 4747 1790 Size: 1801 1156 2573 Fragments arranged by size: 2573 1801 1156 BmgBI CAC'GTC Cuts at: 1318 2352 1318 Size: 1034 4496 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2158 3261 4219 Size: 416 53 83 399 24 741 1103 958 Cuts at: 4219 442 Size: 1753 Fragments arranged by size: 1753 1103 958 741 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5530 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4190 1239 Size: 2951 2579 Bpu10I CC'TnA_GC Cuts at: 2124 2124 Size: 5530 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3655 4520 4818 3655 Size: 865 298 4367 Fragments arranged by size: 4367 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2352 4193 882 Size: 617 853 1841 2219 Fragments arranged by size: 2219 1841 853 617 BsaAI yAC'GTr Cuts at: 493 1204 2431 3321 493 Size: 711 1227 890 2702 Fragments arranged by size: 2702 1227 890 711 BsaBI GATnn'nnATC Cuts at: 5524 5524 Size: 5530 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3337 3719 4354 275 Size: 53 83 186 936 1804 382 635 1451 Fragments arranged by size: 1804 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2389 3963 4361 4635 4782 5193 2389 Size: 1574 398 274 147 411 2726 Fragments arranged by size: 2726 1574 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2351 2381 1178 Size: 30 165 30 948 30 4327 Fragments arranged by size: 4327 948 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2291 1186 Size: 1105 4425 BseYI C'CCAG_C Cuts at: 4733 5229 4733 Size: 496 5034 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2251 1163 Size: 573 515 4442 Fragments arranged by size: 4442 573 515 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2980 3741 4769 1540 Size: 275 186 979 761 1028 2301 Fragments arranged by size: 2301 1028 979 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2297 3591 3676 4747 2046 Size: 251 1294 85 1071 2829 Fragments arranged by size: 2829 1294 1071 251 85 BsiWI C'GTAC_G Cuts at: 2010 2010 Size: 5530 BsmI GAATG_Cn' Cuts at: 3041 3134 3041 Size: 93 5437 BsmBI CGTCTCn'nnnn_ Cuts at: 2358 2358 Size: 5530 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2297 2849 3591 3676 4747 1391 Size: 399 256 251 552 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 552 399 256 251 85 BspEI T'CCGG_A Cuts at: 5193 5193 Size: 5530 BspHI T'CATG_A Cuts at: 2182 3419 5149 2182 Size: 1237 1730 2563 Fragments arranged by size: 2563 1730 1237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2885 844 Size: 2041 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3417 1394 Size: 429 1594 3507 Fragments arranged by size: 3507 1594 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2193 2332 4025 4199 65 Size: 1183 945 139 1693 174 1396 Fragments arranged by size: 1693 1396 1183 945 174 139 BsrFI r'CCGG_y Cuts at: 1700 1810 4174 4361 1700 Size: 110 2364 187 2869 Fragments arranged by size: 2869 2364 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5530 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5530 BssSI C'ACGA_G Cuts at: 2798 3590 4602 2798 Size: 792 1012 3726 Fragments arranged by size: 3726 1012 792 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5530 BstBI TT'CG_AA Cuts at: 4334 4334 Size: 5530 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5530 BstYI r'GATC_y Cuts at: 1972 2802 2860 3227 3612 3629 5081 5167 5179 Size: 830 58 367 385 17 1452 86 12 Cuts at: 5179 5525 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 830 385 367 346 86 58 17 12 Bsu36I CC'TnA_GG Cuts at: 2579 2579 Size: 5530 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4978 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2104 487 Size: 683 56 878 3913 Fragments arranged by size: 3913 878 683 56 BtsI GCAGTG_nn' Cuts at: 3046 3840 3860 3046 Size: 794 20 4716 Fragments arranged by size: 4716 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5530 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5495 DraI TTT'AAA Cuts at: 2834 2834 Size: 5530 DraIII CAC_nnn'GTG Cuts at: 2590 2590 Size: 5530 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2057 4537 809 Size: 507 741 2480 1802 Fragments arranged by size: 2480 1802 741 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2612 2977 Size: 54 1425 50 275 18 264 518 365 Cuts at: 2977 3866 5495 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 518 365 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2977 1537 Size: 275 1165 4090 Fragments arranged by size: 4090 1165 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3460 1353 Size: 2107 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4117 4491 4637 387 Size: 1064 138 2528 374 146 1280 Fragments arranged by size: 2528 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2044 2044 Size: 5530 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3593 4190 4977 5245 1239 Size: 393 1961 597 787 268 1524 Fragments arranged by size: 1961 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2541 2541 Size: 5530 EcoRI G'AATT_C Cuts at: 2189 2839 3217 2189 Size: 650 378 4502 Fragments arranged by size: 4502 650 378 FspI TGC'GCA Cuts at: 4036 4036 Size: 5530 HaeII r_GCGC'y Cuts at: 1678 4677 1678 Size: 2999 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2349 2351 2381 2383 2633 Size: 32 135 32 777 2 30 2 250 Cuts at: 2633 2665 4171 4203 4245 4277 1373 Size: 32 1506 32 42 32 2626 Fragments arranged by size: 2626 1506 777 250 135 42 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2874 3120 669 Size: 988 281 936 246 3079 Fragments arranged by size: 3079 988 936 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5530 HpaI GTT'AAC Cuts at: 3120 3120 Size: 5530 KpnI G_GTAC'C Cuts at: 2855 2855 Size: 5530 MfeI C'AATT_G Cuts at: 3129 3129 Size: 5530 MluI A'CGCG_T Cuts at: 5406 5406 Size: 5530 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2394 4644 4828 1305 Size: 1089 2250 184 2007 Fragments arranged by size: 2250 2007 1089 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 2614 10 Size: 54 1425 343 264 518 2926 Fragments arranged by size: 2926 1425 518 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 2390 3488 3847 4006 518 Size: 648 1224 1098 359 159 2042 Fragments arranged by size: 2042 1224 1098 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2946 3623 4771 5016 1606 Size: 1340 677 1148 245 2120 Fragments arranged by size: 2120 1340 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4978 NdeI CA'TA_TG Cuts at: 387 387 Size: 5530 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5530 NotI GC'GGCC_GC Cuts at: 2977 2977 Size: 5530 NspI r_CATG'y Cuts at: 1132 1229 2274 2348 2525 2888 4433 1132 Size: 97 1045 74 177 363 1545 2229 Fragments arranged by size: 2229 1545 1045 363 177 97 74 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5530 PciI A'CATG_T Cuts at: 2270 2344 4429 2270 Size: 74 2085 3371 Fragments arranged by size: 3371 2085 74 PflMI CCAn_nnn'nTGG Cuts at: 1108 2148 1108 Size: 1040 4490 PfoI T'CCnGG_A Cuts at: 2399 2399 Size: 5530 PmeI GTTT'AAAC Cuts at: 2834 2834 Size: 5530 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3599 3631 5112 5144 3599 Size: 32 1481 32 3985 Fragments arranged by size: 3985 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5530 PsiI TTA'TAA Cuts at: 3100 3100 Size: 5530 PstI C_TGCA'G Cuts at: 830 2253 2545 2882 830 Size: 1423 292 337 3478 Fragments arranged by size: 3478 1423 337 292 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5530 SacI G_AGCT'C Cuts at: 2046 2046 Size: 5530 SalI G'TCGA_C Cuts at: 1936 2872 1936 Size: 936 4594 SbfI CC_TGCA'GG Cuts at: 2882 2882 Size: 5530 ScaI AGT'ACT Cuts at: 2255 2627 3778 5490 2255 Size: 372 1151 1712 2295 Fragments arranged by size: 2295 1712 1151 372 SfcI C'TryA_G Cuts at: 826 1045 2249 2541 2878 4013 4694 4885 826 Size: 219 1204 292 337 1135 681 191 1471 Fragments arranged by size: 1471 1204 1135 681 337 292 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5530 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5530 SmaI CCC'GGG Cuts at: 1872 2381 2857 1872 Size: 509 476 4545 Fragments arranged by size: 4545 509 476 SmlI C'TyrA_G Cuts at: 820 1017 1943 2355 3634 4535 4797 820 Size: 197 926 412 1279 901 262 1553 Fragments arranged by size: 1553 1279 926 901 412 262 197 SnaBI TAC'GTA Cuts at: 493 3321 493 Size: 2828 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5530 SphI G_CATG'C Cuts at: 1132 2525 2888 1132 Size: 1393 363 3774 Fragments arranged by size: 3774 1393 363 SspI AAT'ATT Cuts at: 5 52 3454 5 Size: 47 3402 2081 Fragments arranged by size: 3402 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5530 StyI C'CwwG_G Cuts at: 513 1065 2969 513 Size: 552 1904 3074 Fragments arranged by size: 3074 1904 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3557 3574 3727 3912 4357 325 Size: 1255 1977 17 153 185 445 1498 Fragments arranged by size: 1977 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 2625 3776 Size: 274 80 33 51 1443 276 372 1151 Cuts at: 3776 5488 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1151 372 276 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5490 5522 5490 Size: 32 5498 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5530 XbaI T'CTAG_A Cuts at: 2866 2866 Size: 5530 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2428 2428 Size: 5530 XhoI C'TCGA_G Cuts at: 1943 2355 1943 Size: 412 5118 XmaI C'CCGG_G Cuts at: 1870 2379 2855 1870 Size: 509 476 4545 Fragments arranged by size: 4545 509 476 XmnI GAAnn'nnTTC Cuts at: 3659 3659 Size: 5530 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3338 276 Size: 53 83 186 936 1804 2468 Fragments arranged by size: 2468 1804 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BstXI BstZ17I EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI