(Circular) (Six-base) MAPSORT of: pFN21AB5275.seq Check: 2870 from: 1 to: 6112 pFN21AB5275 6112 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3922 278 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3207 3455 1937 Size: 1270 248 4594 Fragments arranged by size: 4594 1270 248 Acc65I G'GTAC_C Cuts at: 3433 3433 Size: 6112 AclI AA'CG_TT Cuts at: 1563 4239 4612 1563 Size: 2676 373 3063 Fragments arranged by size: 3063 2676 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3280 4175 5559 5827 1632 Size: 1648 895 1384 268 1917 Fragments arranged by size: 1917 1648 1384 895 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5915 AflIII A'CryG_T Cuts at: 1203 2451 5011 5988 1203 Size: 1248 2560 977 1327 Fragments arranged by size: 2560 1327 1248 977 AgeI A'CCGG_T Cuts at: 4943 4943 Size: 6112 AhdI GACnn_n'nnGTC Cuts at: 4841 4841 Size: 6112 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2669 1184 Size: 1485 4627 AlwNI CAG_nnn'CTG Cuts at: 2656 3140 3855 5427 2656 Size: 484 715 1572 3341 Fragments arranged by size: 3341 1572 715 484 ApaI G_GGCC'C Cuts at: 2035 2212 3410 2035 Size: 177 1198 4737 Fragments arranged by size: 4737 1198 177 ApaLI G'TGCA_C Cuts at: 4169 5325 4169 Size: 1156 4956 ApoI r'AATT_y Cuts at: 1493 3421 3647 3799 5908 1493 Size: 1928 226 152 2109 1697 Fragments arranged by size: 2109 1928 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4666 160 Size: 872 3634 1606 Fragments arranged by size: 3634 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 AvaI C'yCGr_G Cuts at: 1870 1943 2079 2721 3361 3437 1870 Size: 73 136 642 640 76 4545 Fragments arranged by size: 4545 642 640 136 76 73 BamHI G'GATC_C Cuts at: 3442 3809 3442 Size: 367 5745 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2083 2441 2645 3433 4888 Size: 325 217 627 296 358 204 788 1455 Cuts at: 4888 618 Size: 1842 Fragments arranged by size: 1842 1455 788 627 358 325 296 217 204 BanII G_rGCy'C Cuts at: 1391 2035 2212 3410 3431 1391 Size: 644 177 1198 21 4072 Fragments arranged by size: 4072 1198 644 177 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5141 BbvCI CC'TCA_GC Cuts at: 2747 2747 Size: 6112 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4303 4337 1903 Size: 34 2366 34 3678 Fragments arranged by size: 3678 2366 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4004 5214 4004 Size: 1210 4902 BclI T'GATC_A Cuts at: 1548 1548 Size: 6112 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2030 2619 2643 Size: 107 122 71 631 605 358 589 24 Cuts at: 2643 4723 136 Size: 2080 1525 Fragments arranged by size: 2080 1525 631 605 589 358 122 107 71 24 BglII A'GATC_T Cuts at: 6107 6107 Size: 6112 BlpI GC'TnA_GC Cuts at: 2861 3529 2861 Size: 668 5444 Bme1580I G_kGCm'C Cuts at: 1790 2035 2212 2291 2648 2877 3200 3410 4173 Size: 245 177 79 357 229 323 210 763 Cuts at: 4173 5329 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 763 357 323 245 229 210 177 79 BmgBI CAC'GTC Cuts at: 1318 2452 2716 2884 1318 Size: 1134 264 168 4546 Fragments arranged by size: 4546 1134 264 168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2230 3843 4801 Size: 416 53 83 399 24 813 1613 958 Cuts at: 4801 442 Size: 1753 Fragments arranged by size: 1753 1613 958 813 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2963 3119 4772 1239 Size: 980 744 156 1653 2579 Fragments arranged by size: 2579 1653 980 744 156 Bpu10I CC'TnA_GC Cuts at: 2747 2747 Size: 6112 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4237 5102 5400 4237 Size: 865 298 4949 Fragments arranged by size: 4949 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2182 4775 882 Size: 617 683 2593 2219 Fragments arranged by size: 2593 2219 683 617 BsaAI yAC'GTr Cuts at: 493 1204 3903 493 Size: 711 2699 2702 Fragments arranged by size: 2702 2699 711 BsaBI GATnn'nnATC Cuts at: 6106 6106 Size: 6112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3919 4301 4936 275 Size: 53 83 186 936 2386 382 635 1451 Fragments arranged by size: 2386 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4545 4943 5217 5364 5775 4545 Size: 398 274 147 411 4882 Fragments arranged by size: 4882 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 2223 2253 1178 Size: 30 165 30 687 30 103 30 5037 Fragments arranged by size: 5037 687 165 103 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2098 2282 2357 2358 2735 1186 Size: 912 184 75 1 377 4563 Fragments arranged by size: 4563 912 377 184 75 1 BseYI C'CCAG_C Cuts at: 2211 2361 2641 2649 2925 3227 3403 5315 5811 Size: 150 280 8 276 302 176 1912 496 Cuts at: 5811 2211 Size: 2512 Fragments arranged by size: 2512 1912 496 302 280 276 176 150 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2722 1163 Size: 573 986 4553 Fragments arranged by size: 4553 986 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3562 4323 5351 1540 Size: 275 186 1561 761 1028 2301 Fragments arranged by size: 2301 1561 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2349 4173 4258 5329 2349 Size: 1824 85 1071 3132 Fragments arranged by size: 3132 1824 1071 85 BsmI GAATG_Cn' Cuts at: 3623 3716 3623 Size: 93 6019 BsmBI CGTCTCn'nnnn_ Cuts at: 2496 2722 2496 Size: 226 5886 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2212 2291 2349 2648 2877 3200 Size: 399 245 177 79 58 299 229 323 Cuts at: 3200 3410 3431 4173 4258 5329 1391 Size: 210 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 323 299 245 229 210 177 85 79 58 21 BspEI T'CCGG_A Cuts at: 5775 5775 Size: 6112 BspHI T'CATG_A Cuts at: 4001 5731 4001 Size: 1730 4382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2361 3336 3467 844 Size: 1517 975 131 3489 Fragments arranged by size: 3489 1517 975 131 BsrBI CCG'CTC Cuts at: 1394 1823 3999 1394 Size: 429 2176 3507 Fragments arranged by size: 3507 2176 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4607 4781 65 Size: 1183 3359 174 1396 Fragments arranged by size: 3359 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2145 4756 4943 1700 Size: 110 335 2611 187 2869 Fragments arranged by size: 2869 2611 335 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6112 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6112 BssSI C'ACGA_G Cuts at: 4172 5184 4172 Size: 1012 5100 BstAPI GCAn_nnn'nTGC Cuts at: 794 2353 794 Size: 1559 4553 BstBI TT'CG_AA Cuts at: 4916 4916 Size: 6112 BstEII G'GTnAC_C Cuts at: 1184 2986 1184 Size: 1802 4310 BstYI r'GATC_y Cuts at: 1972 2847 3093 3442 3809 4194 4211 5663 5749 Size: 875 246 349 367 385 17 1452 86 Cuts at: 5749 5761 6107 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 875 385 367 349 346 246 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2265 3258 2265 Size: 993 5119 BtgI C'CryG_G Cuts at: 513 1065 2877 513 Size: 552 1812 3748 Fragments arranged by size: 3748 1812 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5373 Fragments arranged by size: 5373 683 56 BtsI GCAGTG_nn' Cuts at: 2233 2705 2961 3628 4422 4442 2233 Size: 472 256 667 794 20 3903 Fragments arranged by size: 3903 794 667 472 256 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6077 DraI TTT'AAA Cuts at: 3416 3416 Size: 6112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2402 5119 809 Size: 507 1086 2717 1802 Fragments arranged by size: 2717 1802 1086 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2958 3559 4448 Size: 54 1425 50 275 18 1128 601 889 Cuts at: 4448 6077 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1128 889 601 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3559 1537 Size: 275 1747 4090 Fragments arranged by size: 4090 1747 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4042 1353 Size: 2689 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4699 5073 5219 387 Size: 1064 138 3110 374 146 1280 Fragments arranged by size: 3110 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 2963 3119 3280 4175 4772 5559 Size: 393 587 744 156 161 895 597 787 Cuts at: 5559 5827 1239 Size: 268 1524 Fragments arranged by size: 1524 895 787 744 597 587 393 268 161 156 EcoNI CCTnn'n_nnAGG Cuts at: 2330 2330 Size: 6112 EcoO109I rG'GnC_Cy Cuts at: 2031 3254 3407 2031 Size: 1223 153 4736 Fragments arranged by size: 4736 1223 153 EcoRI G'AATT_C Cuts at: 3421 3799 3421 Size: 378 5734 FspI TGC'GCA Cuts at: 3332 4618 3332 Size: 1286 4826 HaeII r_GCGC'y Cuts at: 1678 2709 2868 5259 1678 Size: 1031 159 2391 2531 Fragments arranged by size: 2531 2391 1031 159 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4753 4785 4827 4859 1373 Size: 32 135 32 3181 32 42 32 2626 Fragments arranged by size: 3181 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3456 3702 669 Size: 988 281 1518 246 3079 Fragments arranged by size: 3079 1518 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6112 HpaI GTT'AAC Cuts at: 3702 3702 Size: 6112 KpnI G_GTAC'C Cuts at: 3437 3437 Size: 6112 MfeI C'AATT_G Cuts at: 3711 3711 Size: 6112 MluI A'CGCG_T Cuts at: 5988 5988 Size: 6112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5226 5410 1305 Size: 3921 184 2007 Fragments arranged by size: 3921 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2960 10 Size: 54 1425 343 1128 3162 Fragments arranged by size: 3162 1425 1128 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2163 2183 2391 2450 4070 4429 4588 Size: 648 997 20 208 59 1620 359 159 Cuts at: 4588 518 Size: 2042 Fragments arranged by size: 2042 1620 997 648 359 208 159 59 20 MspA1I CmG'CkG Cuts at: 1606 2311 2653 2860 2956 2983 3528 4205 5353 Size: 705 342 207 96 27 545 677 1148 Cuts at: 5353 5598 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 705 677 545 342 245 207 96 27 NaeI GCC'GGC Cuts at: 2147 2147 Size: 6112 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5560 NdeI CA'TA_TG Cuts at: 387 387 Size: 6112 NgoMIV G'CCGG_C Cuts at: 2145 2145 Size: 6112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6112 NotI GC'GGCC_GC Cuts at: 3559 3559 Size: 6112 NspI r_CATG'y Cuts at: 1132 1229 2950 3206 3470 5015 1132 Size: 97 1721 256 264 1545 2229 Fragments arranged by size: 2229 1721 1545 264 256 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6112 PciI A'CATG_T Cuts at: 5011 5011 Size: 6112 PflMI CCAn_nnn'nTGG Cuts at: 1108 2656 1108 Size: 1548 4564 PfoI T'CCnGG_A Cuts at: 2196 3096 2196 Size: 900 5212 PmeI GTTT'AAAC Cuts at: 3416 3416 Size: 6112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4181 4213 5694 5726 4181 Size: 32 1481 32 4567 Fragments arranged by size: 4567 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3254 3254 Size: 6112 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6112 PsiI TTA'TAA Cuts at: 3682 3682 Size: 6112 PspOMI G'GGCC_C Cuts at: 2031 2208 3406 2031 Size: 177 1198 4737 Fragments arranged by size: 4737 1198 177 PstI C_TGCA'G Cuts at: 830 2262 2316 2424 2859 3464 830 Size: 1432 54 108 435 605 3478 Fragments arranged by size: 3478 1432 605 435 108 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6112 PvuII CAG'CTG Cuts at: 2653 2860 2983 2653 Size: 207 123 5782 Fragments arranged by size: 5782 207 123 SalI G'TCGA_C Cuts at: 1936 3454 1936 Size: 1518 4594 SbfI CC_TGCA'GG Cuts at: 3464 3464 Size: 6112 ScaI AGT'ACT Cuts at: 4360 6072 4360 Size: 1712 4400 SfcI C'TryA_G Cuts at: 826 1045 2258 2312 2420 2855 3460 4595 5276 Size: 219 1213 54 108 435 605 1135 681 Cuts at: 5276 5467 826 Size: 191 1471 Fragments arranged by size: 1471 1213 1135 681 605 435 219 191 108 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6112 SmaI CCC'GGG Cuts at: 1872 2081 2723 3439 1872 Size: 209 642 716 4545 Fragments arranged by size: 4545 716 642 209 SmlI C'TyrA_G Cuts at: 820 1017 1943 4216 5117 5379 820 Size: 197 926 2273 901 262 1553 Fragments arranged by size: 2273 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3903 493 Size: 3410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6112 SphI G_CATG'C Cuts at: 1132 3470 1132 Size: 2338 3774 SspI AAT'ATT Cuts at: 5 52 4036 5 Size: 47 3984 2081 Fragments arranged by size: 3984 2081 47 StuI AGG'CCT Cuts at: 1517 3396 1517 Size: 1879 4233 StyI C'CwwG_G Cuts at: 513 1065 3551 513 Size: 552 2486 3074 Fragments arranged by size: 3074 2486 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2403 2606 2663 2774 4139 4156 4309 Size: 1255 823 203 57 111 1365 17 153 Cuts at: 4309 4494 4939 325 Size: 185 445 1498 Fragments arranged by size: 1498 1365 1255 823 445 203 185 153 111 57 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4358 6070 96 Size: 274 80 33 51 1443 2381 1712 138 Fragments arranged by size: 2381 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2103 2135 2223 2255 3033 3065 6072 6104 2103 Size: 32 88 32 778 32 3007 32 2111 Fragments arranged by size: 3007 2111 778 88 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6112 XbaI T'CTAG_A Cuts at: 3448 3448 Size: 6112 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2980 2980 Size: 6112 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6112 XmaI C'CCGG_G Cuts at: 1870 2079 2721 3437 1870 Size: 209 642 716 4545 Fragments arranged by size: 4545 716 642 209 XmnI GAAnn'nnTTC Cuts at: 4241 4241 Size: 6112 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3920 276 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI