Restriction map for
pFN21AB5225
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# NO CUT ENZYMES ( 101 enzymes) #
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AccI AccII AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AviII BamHI BbrPI BcgI BciVI
BclI BfrI BglII BsaAI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstBI BstUI BstYI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KspI MfeI MflI
MluI MroI MseI MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1199 GGTACC G^GTACC
AcsI 492 GAATTC [GA]^AATT[CT]
AflIII 1427 ACGTGT A^C[GA][CT]GT
AlwI 1383 GATCC GGATC(4/5)
ApaI 1133 GGGCCC GGGCC^C
ApoI 492 GAATTC [GA]^AATT[CT]
Asp718I 1199 GGTACC G^GTACC
AspHI 726 GAGCTC G[AT]GC[AT]^C
AvrII 513 CCTAGG C^CTAGG
BbsI 1347 GTCTTC GAAGAC(2/6)
BfaI 514 CTAG C^TAG
BlnI 513 CCTAGG C^CTAGG
BlpI 748 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 748 GCTGAGC GC^T[ACGT]AGC
BpuAI 1347 GTCTTC GAAGAC(2/6)
BsaBI 706 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 726 GAGCTC G[AT]GC[AT]^C
BspHI 890 TCATGA T^CATGA
BsrDI 609 GCAATG GCAATG(2/0)
BssSI 442 CTCGTG CACGAG(-5/-1)
BstAPI 18 GCACCGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1638 GGTCACC G^GT[ACGT]ACC
BstPI 1638 GGTCACC G^GT[ACGT]ACC
CelII 748 GCTGAGC GC^T[ACGT]AGC
DpnII 1383 GATC ^GATC
DraIII 1024 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1638 GGTCACC G^GT[ACGT]ACC
EcoRI 492 GAATTC G^AATTC
HindIII 211 AAGCTT A^AGCTT
KpnI 1199 GGTACC GGTAC^C
MaeI 514 CTAG C^TAG
MamI 706 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1383 GATC ^GATC
NcoI 548 CCATGG C^CATGG
NdeII 1383 GATC ^GATC
NspI 23 GCATGC [GA]CATG^[CT]
RcaI 890 TCATGA T^CATGA
SacI 726 GAGCTC GAGCT^C
Sau3AI 1383 GATC ^GATC
ScaI 623 AGTACT AGT^ACT
SmaI 525 CCCGGG CCC^GGG
SphI 23 GCATGC GCATG^C
Tsp509I 493 AATT ^AATT
XcmI 1352 CCATCCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 525 CCCGGG C^CCGGG
XmaI 525 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AatII 364 GACGTC GACGT^C
1820 GACGTC
AlwNI 109 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1601 CAGAGTCTG
AocI 1194 CCTCAGG CC^T[ACGT]AGG
2023 CCTGAGG
AspI 1462 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1599 GACAGAGTC
AvaI 525 CCCGGG C^[CT]CG[GA]G
1177 CCCGAG
BalI 551 TGGCCA TGG^CCA
1297 TGGCCA
BbeI 1704 GGCGCC GGCGC^C
1895 GGCGCC
BsoBI 525 CCCGGG C^[CT]CG[GA]G
1177 CCCGAG
BsrBI 1749 CCGCTC CCGCTC(-3/-3)
180 GAGCGG
BsrFI 120 GCCGGC [GA]^CCGG[CT]
1904 GCCGGC
Bsu36I 1194 CCTCAGG CC^T[ACGT]AGG
2023 CCTGAGG
Cfr10I 120 GCCGGC [GA]^CCGG[CT]
1904 GCCGGC
DsaI 548 CCATGG C^C[GA][CT]GG
765 CCACGG
Eco81I 1194 CCTCAGG CC^T[ACGT]AGG
2023 CCTGAGG
KasI 1704 GGCGCC G^GCGCC
1895 GGCGCC
MluNI 551 TGGCCA TGG^CCA
1297 TGGCCA
MscI 551 TGGCCA TGG^CCA
1297 TGGCCA
NaeI 120 GCCGGC GCC^GGC
1904 GCCGGC
NarI 1704 GGCGCC GG^CGCC
1895 GGCGCC
NgoMIV 120 GCCGGC G^CCGGC
1904 GCCGGC
PflFI 1462 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1599 GACAGAGTC
PflMI 993 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1356 CCAGGTGGTGG
SfoI 1704 GGCGCC GGC^GCC
1895 GGCGCC
Tth111I 1462 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1599 GACAGAGTC
Van91I 993 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1356 CCAGGTGGTGG
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaII
BanI BanII BbvCI BbvI BcnI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I