(Circular) (Six-base) MAPSORT of: pFN21AB5225.seq Check: 8371 from: 1 to: 6808 pFN21AB5225 6808 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2856 3029 2856 Size: 173 6635 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2373 3829 4618 278 Size: 53 83 186 936 837 1456 789 2468 Fragments arranged by size: 2468 1456 936 837 789 186 83 53 AccI GT'mk_AC Cuts at: 1937 4151 1937 Size: 2214 4594 Acc65I G'GTAC_C Cuts at: 3204 4129 3204 Size: 925 5883 AclI AA'CG_TT Cuts at: 1563 4107 4935 5308 1563 Size: 2544 828 373 3063 Fragments arranged by size: 3063 2544 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2597 2618 2984 3146 3567 4871 6255 6523 Size: 965 21 366 162 421 1304 1384 268 Cuts at: 6523 1632 Size: 1917 Fragments arranged by size: 1917 1384 1304 965 421 366 268 162 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6611 AflIII A'CryG_T Cuts at: 1203 3432 5707 6684 1203 Size: 2229 2275 977 1327 Fragments arranged by size: 2275 2229 1327 977 AgeI A'CCGG_T Cuts at: 5639 5639 Size: 6808 AhdI GACnn_n'nnGTC Cuts at: 5537 5537 Size: 6808 AleI CACnn'nnGTG Cuts at: 1166 3512 1166 Size: 2346 4462 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6808 AlwNI CAG_nnn'CTG Cuts at: 2119 3611 4551 6123 2119 Size: 1492 940 1572 2804 Fragments arranged by size: 2804 1572 1492 940 ApaI G_GGCC'C Cuts at: 3142 3142 Size: 6808 ApaLI G'TGCA_C Cuts at: 4865 6021 4865 Size: 1156 5652 ApoI r'AATT_y Cuts at: 1493 2497 4117 4343 4495 6604 1493 Size: 1004 1620 226 152 2109 1697 Fragments arranged by size: 2109 1697 1620 1004 226 152 AseI AT'TA_AT Cuts at: 160 1032 5362 160 Size: 872 4330 1606 Fragments arranged by size: 4330 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6808 AvaI C'yCGr_G Cuts at: 1870 1943 2530 3182 4133 1870 Size: 73 587 652 951 4545 Fragments arranged by size: 4545 951 652 587 73 AvrII C'CTAG_G Cuts at: 2518 2518 Size: 6808 BamHI G'GATC_C Cuts at: 4138 4505 4138 Size: 367 6441 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3041 3204 3616 3709 3900 Size: 325 217 627 1254 163 412 93 191 Cuts at: 3900 4129 5584 618 Size: 229 1455 1842 Fragments arranged by size: 1842 1455 1254 627 412 325 229 217 191 163 93 BanII G_rGCy'C Cuts at: 1391 2735 3142 3853 4027 4127 1391 Size: 1344 407 711 174 100 4072 Fragments arranged by size: 4072 1344 711 407 174 100 BbeI G_GCGC'C Cuts at: 3713 3904 3713 Size: 191 6617 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3345 928 Size: 971 1446 4391 Fragments arranged by size: 4391 1446 971 BbvCI CC'TCA_GC Cuts at: 2759 3191 3888 2759 Size: 432 697 5679 Fragments arranged by size: 5679 697 432 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4999 5033 1903 Size: 34 3062 34 3678 Fragments arranged by size: 3678 3062 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4700 5910 4700 Size: 1210 5598 BclI T'GATC_A Cuts at: 1548 1548 Size: 6808 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2135 2523 3718 Size: 107 122 71 631 605 463 388 1195 Cuts at: 3718 3898 4031 5419 136 Size: 180 133 1388 1525 Fragments arranged by size: 1525 1388 1195 631 605 463 388 180 133 122 107 71 BglII A'GATC_T Cuts at: 6803 6803 Size: 6808 BlpI GC'TnA_GC Cuts at: 2754 4225 2754 Size: 1471 5337 Bme1580I G_kGCm'C Cuts at: 1790 3046 3142 3519 4869 6025 1790 Size: 1256 96 377 1350 1156 2573 Fragments arranged by size: 2573 1350 1256 1156 377 96 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6808 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3668 3959 4539 Size: 416 53 83 399 24 2251 291 580 Cuts at: 4539 5497 442 Size: 958 1753 Fragments arranged by size: 2251 1753 958 580 416 399 291 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6808 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3034 3034 Size: 6808 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2141 2381 2423 2717 3026 3435 3446 3537 Size: 902 240 42 294 309 409 11 91 Cuts at: 3537 3543 3750 5468 1239 Size: 6 207 1718 2579 Fragments arranged by size: 2579 1718 902 409 309 294 240 207 91 42 11 6 Bpu10I CC'TnA_GC Cuts at: 2759 3191 3888 2759 Size: 432 697 5679 Fragments arranged by size: 5679 697 432 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2453 2957 3032 4933 5798 6096 2453 Size: 504 75 1901 865 298 3165 Fragments arranged by size: 3165 1901 865 504 298 75 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2066 2936 3748 4094 5471 882 Size: 617 567 870 812 346 1377 2219 Fragments arranged by size: 2219 1377 870 812 617 567 346 BsaAI yAC'GTr Cuts at: 493 1204 4599 493 Size: 711 3395 2702 Fragments arranged by size: 3395 2702 711 BsaBI GATnn'nnATC Cuts at: 2715 6802 2715 Size: 4087 2721 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2370 3372 3710 3826 Size: 53 83 186 936 837 1002 338 116 Cuts at: 3826 3901 4615 4997 5632 275 Size: 75 714 382 635 1451 Fragments arranged by size: 1451 1002 936 837 714 635 382 338 186 116 83 75 53 BsaWI w'CCGG_w Cuts at: 5241 5639 5913 6060 6471 5241 Size: 398 274 147 411 5578 Fragments arranged by size: 5578 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3523 3553 1178 Size: 30 165 30 2120 30 4433 Fragments arranged by size: 4433 2120 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 2417 2597 2600 2603 2606 3023 3251 Size: 1228 3 180 3 3 3 417 228 Cuts at: 3251 3748 4035 1186 Size: 497 287 3959 Fragments arranged by size: 3959 1228 497 417 287 228 180 3 3 3 3 BseYI C'CCAG_C Cuts at: 2857 2948 3263 3330 3523 3571 3929 3959 4057 Size: 91 315 67 193 48 358 30 98 Cuts at: 4057 6011 6507 2857 Size: 1954 496 3158 Fragments arranged by size: 3158 1954 496 358 315 193 98 91 67 48 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2006 3187 3969 1163 Size: 573 270 1181 782 4002 Fragments arranged by size: 4002 1181 782 573 270 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4258 5019 6047 1540 Size: 275 186 2257 761 1028 2301 Fragments arranged by size: 2301 2257 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2735 4869 4954 6025 2735 Size: 2134 85 1071 3518 Fragments arranged by size: 3518 2134 1071 85 BsmI GAATG_Cn' Cuts at: 4319 4412 4319 Size: 93 6715 BsmBI CGTCTCn'nnnn_ Cuts at: 2564 2696 2975 2564 Size: 132 279 6397 Fragments arranged by size: 6397 279 132 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2735 3046 3142 3519 3853 4027 4127 Size: 399 945 311 96 377 334 174 100 Cuts at: 4127 4869 4954 6025 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 945 742 399 377 334 311 174 100 96 85 BspEI T'CCGG_A Cuts at: 6471 6471 Size: 6808 BspHI T'CATG_A Cuts at: 2895 4697 6427 2895 Size: 1802 1730 3276 Fragments arranged by size: 3276 1802 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2095 2106 2712 2856 3029 4163 844 Size: 1251 11 606 144 173 1134 3489 Fragments arranged by size: 3489 1251 1134 606 173 144 11 BsrBI CCG'CTC Cuts at: 1394 1823 2187 3756 4695 1394 Size: 429 364 1569 939 3507 Fragments arranged by size: 3507 1569 939 429 364 BsrDI GCAATG_nn' Cuts at: 65 1248 2621 5303 5477 65 Size: 1183 1373 2682 174 1396 Fragments arranged by size: 2682 1396 1373 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2125 3909 5452 5639 1700 Size: 110 315 1784 1543 187 2869 Fragments arranged by size: 2869 1784 1543 315 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6808 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6808 BssSI C'ACGA_G Cuts at: 2447 4868 5880 2447 Size: 2421 1012 3375 Fragments arranged by size: 3375 2421 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2029 794 Size: 1235 5573 BstBI TT'CG_AA Cuts at: 5612 5612 Size: 6808 BstEII G'GTnAC_C Cuts at: 1184 3643 1184 Size: 2459 4349 BstXI CCAn_nnnn'nTGG Cuts at: 2245 2566 3283 3364 2245 Size: 321 717 81 5689 Fragments arranged by size: 5689 717 321 81 BstYI r'GATC_y Cuts at: 1972 4138 4505 4890 4907 6359 6445 6457 6803 Size: 2166 367 385 17 1452 86 12 346 Cuts at: 6803 1972 Size: 1977 Fragments arranged by size: 2166 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3200 4029 3200 Size: 829 5979 BtgI C'CryG_G Cuts at: 513 1065 2553 2770 513 Size: 552 1488 217 4551 Fragments arranged by size: 4551 1488 552 217 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2702 3256 487 Size: 683 56 1476 554 4039 Fragments arranged by size: 4039 1476 683 554 56 BtsI GCAGTG_nn' Cuts at: 3507 4324 5118 5138 3507 Size: 817 794 20 5177 Fragments arranged by size: 5177 817 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6808 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6773 DraI TTT'AAA Cuts at: 4112 4112 Size: 6808 DraIII CAC_nnn'GTG Cuts at: 3034 3034 Size: 6808 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5815 809 Size: 507 4499 1802 Fragments arranged by size: 4499 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2127 2340 2556 Size: 54 1425 50 275 18 297 213 216 Cuts at: 2556 2773 3231 3302 4255 5144 6773 8 Size: 217 458 71 953 889 1629 43 Fragments arranged by size: 1629 1425 953 889 458 297 275 217 216 213 71 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4255 1537 Size: 275 2443 4090 Fragments arranged by size: 4090 2443 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2006 2594 2919 2939 3413 3449 4738 1353 Size: 653 588 325 20 474 36 1289 3423 Fragments arranged by size: 3423 1289 653 588 474 325 36 20 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5395 5769 5915 387 Size: 1064 138 3806 374 146 1280 Fragments arranged by size: 3806 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2733 2733 Size: 6808 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2141 2381 2423 2597 2618 2717 2984 Size: 393 509 240 42 174 21 99 267 Cuts at: 2984 3026 3146 3435 3446 3537 3543 3567 3750 Size: 42 120 289 11 91 6 24 183 Cuts at: 3750 4871 5468 6255 6523 1239 Size: 1121 597 787 268 1524 Fragments arranged by size: 1524 1121 787 597 509 393 289 268 267 240 183 174 120 99 91 42 42 24 21 11 6 EcoNI CCTnn'n_nnAGG Cuts at: 2090 2102 3158 2090 Size: 12 1056 5740 Fragments arranged by size: 5740 1056 12 EcoO109I rG'GnC_Cy Cuts at: 2095 2952 3138 3151 3287 3804 4045 2095 Size: 857 186 13 136 517 241 4858 Fragments arranged by size: 4858 857 517 241 186 136 13 EcoRI G'AATT_C Cuts at: 2497 4117 4495 2497 Size: 1620 378 4810 Fragments arranged by size: 4810 1620 378 FspI TGC'GCA Cuts at: 5314 5314 Size: 6808 HaeII r_GCGC'y Cuts at: 1678 3386 3706 3713 3904 5955 1678 Size: 1708 320 7 191 2051 2531 Fragments arranged by size: 2531 2051 1708 320 191 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3329 3361 5449 5481 5523 Size: 32 135 32 1757 32 2088 32 42 Cuts at: 5523 5555 1373 Size: 32 2626 Fragments arranged by size: 2626 2088 1757 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4152 4398 669 Size: 988 281 2214 246 3079 Fragments arranged by size: 3079 2214 988 281 246 HindIII A'AGCT_T Cuts at: 748 2216 748 Size: 1468 5340 HpaI GTT'AAC Cuts at: 4398 4398 Size: 6808 KasI G'GCGC_C Cuts at: 3709 3900 3709 Size: 191 6617 KpnI G_GTAC'C Cuts at: 3208 4133 3208 Size: 925 5883 MfeI C'AATT_G Cuts at: 4407 4407 Size: 6808 MluI A'CGCG_T Cuts at: 6684 6684 Size: 6808 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2478 3045 5922 6106 1305 Size: 1173 567 2877 184 2007 Fragments arranged by size: 2877 2007 1173 567 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2558 3304 10 Size: 54 1425 343 726 746 3514 Fragments arranged by size: 3514 1425 746 726 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 3362 3512 4766 5125 5284 518 Size: 648 1077 1119 150 1254 359 159 2042 Fragments arranged by size: 2042 1254 1119 1077 648 359 159 150 MspA1I CmG'CkG Cuts at: 1606 2020 2038 2078 2116 2326 3334 3452 3668 Size: 414 18 40 38 210 1008 118 216 Cuts at: 3668 4224 4901 6049 6294 1606 Size: 556 677 1148 245 2120 Fragments arranged by size: 2120 1148 1008 677 556 414 245 216 210 118 40 38 18 NaeI GCC'GGC Cuts at: 2127 3911 2127 Size: 1784 5024 NarI GG'CG_CC Cuts at: 3710 3901 3710 Size: 191 6617 NcoI C'CATG_G Cuts at: 513 1065 2553 513 Size: 552 1488 4768 Fragments arranged by size: 4768 1488 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6808 NgoMIV G'CCGG_C Cuts at: 2125 3909 2125 Size: 1784 5024 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6808 NotI GC'GGCC_GC Cuts at: 4255 4255 Size: 6808 NspI r_CATG'y Cuts at: 1132 1229 2032 4166 5711 1132 Size: 97 803 2134 1545 2229 Fragments arranged by size: 2229 2134 1545 803 97 PasI CC'CwG_GG Cuts at: 1348 2817 3800 1348 Size: 1469 983 4356 Fragments arranged by size: 4356 1469 983 PciI A'CATG_T Cuts at: 5707 5707 Size: 6808 PflMI CCAn_nnn'nTGG Cuts at: 1108 3004 3367 1108 Size: 1896 363 4549 Fragments arranged by size: 4549 1896 363 PfoI T'CCnGG_A Cuts at: 2694 3462 2694 Size: 768 6040 PmeI GTTT'AAAC Cuts at: 4112 4112 Size: 6808 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4877 4909 6390 6422 4877 Size: 32 1481 32 5263 Fragments arranged by size: 5263 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2095 3287 3804 4045 2095 Size: 1192 517 241 4858 Fragments arranged by size: 4858 1192 517 241 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6808 PsiI TTA'TAA Cuts at: 4378 4378 Size: 6808 PspOMI G'GGCC_C Cuts at: 3138 3138 Size: 6808 PstI C_TGCA'G Cuts at: 830 2106 2166 2178 2379 3150 4160 830 Size: 1276 60 12 201 771 1010 3478 Fragments arranged by size: 3478 1276 1010 771 201 60 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6808 PvuII CAG'CTG Cuts at: 2020 2038 2326 3334 3452 3668 2020 Size: 18 288 1008 118 216 5160 Fragments arranged by size: 5160 1008 288 216 118 18 SacI G_AGCT'C Cuts at: 2735 2735 Size: 6808 SalI G'TCGA_C Cuts at: 1936 4150 1936 Size: 2214 4594 SanDI GG'GwC_CC Cuts at: 3287 3804 4045 3287 Size: 517 241 6050 Fragments arranged by size: 6050 517 241 SbfI CC_TGCA'GG Cuts at: 4160 4160 Size: 6808 ScaI AGT'ACT Cuts at: 2630 5056 6768 2630 Size: 2426 1712 2670 Fragments arranged by size: 2670 2426 1712 SfcI C'TryA_G Cuts at: 826 1045 2102 2162 2174 2375 3146 4156 5291 Size: 219 1057 60 12 201 771 1010 1135 Cuts at: 5291 5972 6163 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1057 1010 771 681 219 201 191 60 12 SfoI GGC'GCC Cuts at: 3711 3902 3711 Size: 191 6617 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6808 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6808 SmaI CCC'GGG Cuts at: 1872 2532 4135 1872 Size: 660 1603 4545 Fragments arranged by size: 4545 1603 660 SmlI C'TyrA_G Cuts at: 820 1017 1943 2468 2972 3047 4912 5813 6075 Size: 197 926 525 504 75 1865 901 262 Cuts at: 6075 820 Size: 1553 Fragments arranged by size: 1865 1553 926 901 525 504 262 197 75 SnaBI TAC'GTA Cuts at: 493 4599 493 Size: 4106 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6808 SphI G_CATG'C Cuts at: 1132 2032 4166 1132 Size: 900 2134 3774 Fragments arranged by size: 3774 2134 900 SspI AAT'ATT Cuts at: 5 52 4732 5 Size: 47 4680 2081 Fragments arranged by size: 4680 2081 47 StuI AGG'CCT Cuts at: 1517 2168 2294 2517 2888 3441 1517 Size: 651 126 223 371 553 4884 Fragments arranged by size: 4884 651 553 371 223 126 StyI C'CwwG_G Cuts at: 513 1065 2295 2518 2553 2658 3576 3594 3639 Size: 552 1230 223 35 105 918 18 45 Cuts at: 3639 4247 513 Size: 608 3074 Fragments arranged by size: 3074 1230 918 608 552 223 105 45 35 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2078 2822 3277 4835 4852 5005 5190 Size: 1255 498 744 455 1558 17 153 185 Cuts at: 5190 5635 325 Size: 445 1498 Fragments arranged by size: 1558 1498 1255 744 498 455 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2628 5054 6766 Size: 274 80 33 51 1443 651 2426 1712 Cuts at: 6766 96 Size: 138 Fragments arranged by size: 2426 1712 1443 651 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3523 3555 3637 3669 6768 6800 3523 Size: 32 82 32 3099 32 3531 Fragments arranged by size: 3531 3099 82 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3470 3607 1316 Size: 2154 137 4517 Fragments arranged by size: 4517 2154 137 XbaI T'CTAG_A Cuts at: 4144 4144 Size: 6808 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3364 3364 Size: 6808 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6808 XmaI C'CCGG_G Cuts at: 1870 2530 4133 1870 Size: 660 1603 4545 Fragments arranged by size: 4545 1603 660 XmnI GAAnn'nnTTC Cuts at: 4937 4937 Size: 6808 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2371 3827 4616 276 Size: 53 83 186 936 837 1456 789 2468 Fragments arranged by size: 2468 1456 936 837 789 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI