Restriction map for
pFN21AB5169
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII AclI AcsI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsgI BsiEI BsiWI BsmBI BspDI
BspHI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KspI MamI MfeI MflI MluI
MspA1I MunI NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoII XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 592 GGTACC G^GTACC
AccI 403 GTCTAC GT^[AC][GT]AC
AcyI 1091 GGCGCC G[GA]^CG[CT]C
AflIII 1235 ACATGT A^C[GA][CT]GT
AlwNI 7 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 893 GGGCCC GGGCC^C
Asp718I 592 GGTACC G^GTACC
BalI 1191 TGGCCA TGG^CCA
BbeI 1091 GGCGCC GGCGC^C
BfaI 574 CTAG C^TAG
BglI 564 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1091 GGCGCC G[GA]^CG[CT]C
BsmI 627 GCATTC GAATGC(1/-1)
Bsp1407I 1238 TGTACA T^GTACA
BspLU11I 1235 ACATGT A^CATGT
BspMI 62 ACCTGC ACCTGC(4/8)
BsrDI 120 CATTGC GCAATG(2/0)
BsrFI 782 ACCGGC [GA]^CCGG[CT]
BsrGI 1238 TGTACA T^GTACA
BsrI 6 CCAGT ACTGG(1/-1)
BstXI 20 CCAACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 782 ACCGGC [GA]^CCGG[CT]
DsaI 429 CCGTGG C^C[GA][CT]GG
EaeI 1191 TGGCCA [CT]^GGCC[GA]
Hin1I 1091 GGCGCC G[GA]^CG[CT]C
HphI 967 GGTGA GGTGA(8/7)
KasI 1091 GGCGCC G^GCGCC
KpnI 592 GGTACC GGTAC^C
MaeI 574 CTAG C^TAG
MaeII 530 ACGT A^CGT
MluNI 1191 TGGCCA TGG^CCA
MscI 1191 TGGCCA TGG^CCA
MseI 257 TTAA T^TAA
NarI 1091 GGCGCC GG^CGCC
NruI 804 TCGCGA TCG^CGA
PaeR7I 152 CTCGAG C^TCGAG
SacI 1000 GAGCTC GAGCT^C
SfcI 405 CTACAG C^T[GA][CT]AG
SfoI 1091 GGCGCC GGC^GCC
SmaI 776 CCCGGG CCC^GGG
SmlI 152 CTCGAG C^T[CT][GA]AG
SspBI 1238 TGTACA T^GTACA
StuI 582 AGGCCT AGG^CCT
TfiI 346 GAATC G^A[AT]TC
Tru9I 257 TTAA T^TAA
Tsp509I 331 AATT ^AATT
TspRI 1152 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 152 CTCGAG C^TCGAG
XmaCI 776 CCCGGG C^CCGGG
XmaI 776 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccIII 356 TCCGGA T^CCGGA
1275 TCCGGA
AspHI 1000 GAGCTC G[AT]GC[AT]^C
1129 GAGCAC
BanI 592 GGTACC G^G[CT][GA]CC
1091 GGCGCC
BseAI 356 TCCGGA T^CCGGA
1275 TCCGGA
BsiHKAI 1000 GAGCTC G[AT]GC[AT]^C
1129 GAGCAC
BspEI 356 TCCGGA T^CCGGA
1275 TCCGGA
BssSI 457 CACGAG CACGAG(-5/-1)
173 CTCGTG
DdeI 290 CTCAG C^T[ACGT]AG
1250 CTGAG
EarI 313 CTCTTC CTCTTC(1/4)
599 GAAGAG
HgaI 764 GACGC GACGC(5/10)
970 GACGC
Ksp632I 313 CTCTTC CTCTTC(1/4)
599 GAAGAG
MroI 356 TCCGGA T^CCGGA
1275 TCCGGA
MslI 487 CACGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1236 CATGTACATG
NspI 467 ACATGC [GA]CATG^[CT]
1235 ACATGT
SfaNI 157 GATGC GCATC(5/9)
298 GATGC
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeIII
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI TaqI TseI Tsp45I
TthHB8I