(Circular) (Six-base) MAPSORT of: pFN21AB5169.seq Check: 5404 from: 1 to: 6016 pFN21AB5169 6016 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3826 278 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2409 3359 1937 Size: 472 950 4594 Fragments arranged by size: 4594 950 472 Acc65I G'GTAC_C Cuts at: 2597 3337 2597 Size: 740 5276 AclI AA'CG_TT Cuts at: 1563 4143 4516 1563 Size: 2580 373 3063 Fragments arranged by size: 3063 2580 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4079 5463 5731 1632 Size: 2447 1384 268 1917 Fragments arranged by size: 2447 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5819 AflIII A'CryG_T Cuts at: 1203 3240 4915 5892 1203 Size: 2037 1675 977 1327 Fragments arranged by size: 2037 1675 1327 977 AgeI A'CCGG_T Cuts at: 4847 4847 Size: 6016 AhdI GACnn_n'nnGTC Cuts at: 4745 4745 Size: 6016 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6016 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6016 AlwNI CAG_nnn'CTG Cuts at: 2017 3759 5331 2017 Size: 1742 1572 2702 Fragments arranged by size: 2702 1742 1572 ApaI G_GGCC'C Cuts at: 2902 2902 Size: 6016 ApaLI G'TGCA_C Cuts at: 4073 5229 4073 Size: 1156 4860 ApoI r'AATT_y Cuts at: 1493 3325 3551 3703 5812 1493 Size: 1832 226 152 2109 1697 Fragments arranged by size: 2109 1832 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4570 160 Size: 872 3538 1606 Fragments arranged by size: 3538 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 AvaI C'yCGr_G Cuts at: 1870 1943 2157 2190 2781 3008 3180 3341 1870 Size: 73 214 33 591 227 172 161 4545 Fragments arranged by size: 4545 591 227 214 172 161 73 33 BamHI G'GATC_C Cuts at: 3346 3713 3346 Size: 367 5649 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2597 3096 3337 4792 618 Size: 325 217 627 810 499 241 1455 1842 Fragments arranged by size: 1842 1455 810 627 499 325 241 217 BanII G_rGCy'C Cuts at: 1391 2902 2961 3009 3335 1391 Size: 1511 59 48 326 4072 Fragments arranged by size: 4072 1511 326 59 48 BbeI G_GCGC'C Cuts at: 3100 3100 Size: 6016 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5045 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4207 4241 1903 Size: 34 2270 34 3678 Fragments arranged by size: 3678 2270 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3908 5118 3908 Size: 1210 4806 BclI T'GATC_A Cuts at: 1548 1548 Size: 6016 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2575 4627 136 Size: 107 122 71 631 605 903 2052 1525 Fragments arranged by size: 2052 1525 903 631 605 122 107 71 BglII A'GATC_T Cuts at: 6011 6011 Size: 6016 BlpI GC'TnA_GC Cuts at: 3433 3433 Size: 6016 Bme1580I G_kGCm'C Cuts at: 1790 2902 4077 5233 1790 Size: 1112 1175 1156 2573 Fragments arranged by size: 2573 1175 1156 1112 BmgBI CAC'GTC Cuts at: 1318 2536 1318 Size: 1218 4798 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2005 3747 4705 Size: 416 53 83 399 24 588 1742 958 Cuts at: 4705 442 Size: 1753 Fragments arranged by size: 1753 1742 958 588 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6016 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2606 2606 Size: 6016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2584 2818 2982 3233 4676 1239 Size: 860 485 234 164 251 1443 2579 Fragments arranged by size: 2579 1443 860 485 251 234 164 Bpu10I CC'TnA_GC Cuts at: 2295 2295 Size: 6016 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4141 5006 5304 4141 Size: 865 298 4853 Fragments arranged by size: 4853 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4679 882 Size: 617 3180 2219 Fragments arranged by size: 3180 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3807 493 Size: 711 2603 2702 Fragments arranged by size: 2702 2603 711 BsaBI GATnn'nnATC Cuts at: 6010 6010 Size: 6016 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3097 3823 4205 4840 Size: 53 83 186 936 1564 726 382 635 Cuts at: 4840 275 Size: 1451 Fragments arranged by size: 1564 1451 936 726 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2361 2865 3078 3280 4449 4847 5121 5268 5679 Size: 504 213 202 1169 398 274 147 411 Cuts at: 5679 2361 Size: 2698 Fragments arranged by size: 2698 1169 504 411 398 274 213 202 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2462 2492 3275 3305 1178 Size: 30 165 30 1059 30 783 30 3889 Fragments arranged by size: 3889 1059 783 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2160 2210 2598 2622 2834 2939 1186 Size: 974 50 388 24 212 105 4263 Fragments arranged by size: 4263 974 388 212 105 50 24 BseYI C'CCAG_C Cuts at: 5219 5715 5219 Size: 496 5520 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5443 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3466 4227 5255 1540 Size: 275 186 1465 761 1028 2301 Fragments arranged by size: 2301 1465 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3009 3138 4077 4162 5233 3009 Size: 129 939 85 1071 3792 Fragments arranged by size: 3792 1071 939 129 85 BsmI GAATG_Cn' Cuts at: 2632 3527 3620 2632 Size: 895 93 5028 Fragments arranged by size: 5028 895 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2902 2961 3009 3138 3335 4077 4162 Size: 399 1112 59 48 129 197 742 85 Cuts at: 4162 5233 1391 Size: 1071 2174 Fragments arranged by size: 2174 1112 1071 742 399 197 129 85 59 48 BspEI T'CCGG_A Cuts at: 2361 3280 5679 2361 Size: 919 2399 2698 Fragments arranged by size: 2698 2399 919 BspHI T'CATG_A Cuts at: 3905 5635 3905 Size: 1730 4286 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2076 3371 844 Size: 1232 1295 3489 Fragments arranged by size: 3489 1295 1232 BsrBI CCG'CTC Cuts at: 1394 1823 2233 2719 2734 2778 2824 2842 2896 Size: 429 410 486 15 44 46 18 54 Cuts at: 2896 2911 3094 3151 3163 3903 1394 Size: 15 183 57 12 740 3507 Fragments arranged by size: 3507 740 486 429 410 183 57 54 46 44 18 15 15 12 BsrDI GCAATG_nn' Cuts at: 65 1248 2124 4511 4685 65 Size: 1183 876 2387 174 1396 Fragments arranged by size: 2387 1396 1183 876 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2787 4660 4847 1700 Size: 110 977 1873 187 2869 Fragments arranged by size: 2869 1873 977 187 110 BsrGI T'GTAC_A Cuts at: 96 3243 96 Size: 3147 2869 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6016 BssSI C'ACGA_G Cuts at: 2178 2462 4076 5088 2178 Size: 284 1614 1012 3106 Fragments arranged by size: 3106 1614 1012 284 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6016 BstBI TT'CG_AA Cuts at: 4820 4820 Size: 6016 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6016 BstXI CCAn_nnnn'nTGG Cuts at: 2032 2032 Size: 6016 BstYI r'GATC_y Cuts at: 1972 3346 3713 4098 4115 5567 5653 5665 6011 Size: 1374 367 385 17 1452 86 12 346 Cuts at: 6011 1972 Size: 1977 Fragments arranged by size: 1977 1452 1374 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2434 513 Size: 552 1369 4095 Fragments arranged by size: 4095 1369 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2658 487 Size: 683 56 1432 3845 Fragments arranged by size: 3845 1432 683 56 BtsI GCAGTG_nn' Cuts at: 3163 3532 4326 4346 3163 Size: 369 794 20 4833 Fragments arranged by size: 4833 794 369 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6016 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5981 DraI TTT'AAA Cuts at: 3320 3320 Size: 6016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5023 809 Size: 507 3707 1802 Fragments arranged by size: 3707 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3196 3463 4352 Size: 54 1425 50 275 18 1366 267 889 Cuts at: 4352 5981 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1366 889 275 267 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3463 1537 Size: 275 1651 4090 Fragments arranged by size: 4090 1651 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2324 2599 3946 1353 Size: 971 275 1347 3423 Fragments arranged by size: 3423 1347 971 275 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2806 2969 3085 4603 4977 5123 Size: 1064 138 1217 163 116 1518 374 146 Cuts at: 5123 387 Size: 1280 Fragments arranged by size: 1518 1280 1217 1064 374 163 146 138 116 EcoICRI GAG'CTC Cuts at: 3007 3007 Size: 6016 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2584 2818 2982 3233 4079 4676 Size: 393 467 485 234 164 251 846 597 Cuts at: 4676 5463 5731 1239 Size: 787 268 1524 Fragments arranged by size: 1524 846 787 597 485 467 393 268 251 234 164 EcoO109I rG'GnC_Cy Cuts at: 2152 2272 2524 2568 2152 Size: 120 252 44 5600 Fragments arranged by size: 5600 252 120 44 EcoRI G'AATT_C Cuts at: 3325 3703 3325 Size: 378 5638 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2335 2335 Size: 6016 FspI TGC'GCA Cuts at: 4522 4522 Size: 6016 HaeII r_GCGC'y Cuts at: 1678 2800 2833 3100 5163 1678 Size: 1122 33 267 2063 2531 Fragments arranged by size: 2531 2063 1122 267 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2462 2494 2636 2668 4657 Size: 32 135 32 890 32 142 32 1989 Cuts at: 4657 4689 4731 4763 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1989 890 142 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3360 3606 669 Size: 988 281 1422 246 3079 Fragments arranged by size: 3079 1422 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6016 HpaI GTT'AAC Cuts at: 3606 3606 Size: 6016 KasI G'GCGC_C Cuts at: 3096 3096 Size: 6016 KpnI G_GTAC'C Cuts at: 2601 3341 2601 Size: 740 5276 MfeI C'AATT_G Cuts at: 3615 3615 Size: 6016 MluI A'CGCG_T Cuts at: 5892 5892 Size: 6016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2198 5130 5314 1305 Size: 893 2932 184 2007 Fragments arranged by size: 2932 2007 893 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3198 10 Size: 54 1425 343 1366 2828 Fragments arranged by size: 2828 1425 1366 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2496 3245 3974 4333 4492 518 Size: 648 1330 749 729 359 159 2042 Fragments arranged by size: 2042 1330 749 729 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3432 4109 5257 5502 1606 Size: 1826 677 1148 245 2120 Fragments arranged by size: 2120 1826 1148 677 245 NarI GG'CG_CC Cuts at: 3097 3097 Size: 6016 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5464 NdeI CA'TA_TG Cuts at: 387 387 Size: 6016 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6016 NotI GC'GGCC_GC Cuts at: 3463 3463 Size: 6016 NruI TCG'CGA Cuts at: 2811 2811 Size: 6016 NspI r_CATG'y Cuts at: 1132 1229 2476 3244 3374 4919 1132 Size: 97 1247 768 130 1545 2229 Fragments arranged by size: 2229 1545 1247 768 130 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6016 PciI A'CATG_T Cuts at: 3240 4915 3240 Size: 1675 4341 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6016 PmeI GTTT'AAAC Cuts at: 3320 3320 Size: 6016 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4085 4117 5598 5630 4085 Size: 32 1481 32 4471 Fragments arranged by size: 4471 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2152 2272 2524 2152 Size: 120 252 5644 Fragments arranged by size: 5644 252 120 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6016 PsiI TTA'TAA Cuts at: 3586 3586 Size: 6016 PspOMI G'GGCC_C Cuts at: 2898 2898 Size: 6016 PstI C_TGCA'G Cuts at: 830 3368 830 Size: 2538 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6016 SacI G_AGCT'C Cuts at: 3009 3009 Size: 6016 SalI G'TCGA_C Cuts at: 1936 3358 1936 Size: 1422 4594 SanDI GG'GwC_CC Cuts at: 2272 2272 Size: 6016 SbfI CC_TGCA'GG Cuts at: 3368 3368 Size: 6016 ScaI AGT'ACT Cuts at: 4264 5976 4264 Size: 1712 4304 SfcI C'TryA_G Cuts at: 826 1045 2410 3364 4499 5180 5371 826 Size: 219 1365 954 1135 681 191 1471 Fragments arranged by size: 1471 1365 1135 954 681 219 191 SfoI GGC'GCC Cuts at: 3098 3098 Size: 6016 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6016 SmaI CCC'GGG Cuts at: 1872 2783 3343 1872 Size: 911 560 4545 Fragments arranged by size: 4545 911 560 SmlI C'TyrA_G Cuts at: 820 1017 1943 2157 4120 5021 5283 820 Size: 197 926 214 1963 901 262 1553 Fragments arranged by size: 1963 1553 926 901 262 214 197 SnaBI TAC'GTA Cuts at: 493 3807 493 Size: 3314 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6016 SphI G_CATG'C Cuts at: 1132 3374 1132 Size: 2242 3774 SspI AAT'ATT Cuts at: 5 52 3940 5 Size: 47 3888 2081 Fragments arranged by size: 3888 2081 47 StuI AGG'CCT Cuts at: 1517 2589 1517 Size: 1072 4944 StyI C'CwwG_G Cuts at: 513 1065 3455 513 Size: 552 2390 3074 Fragments arranged by size: 3074 2390 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4043 4060 4213 4398 4843 325 Size: 1255 2463 17 153 185 445 1498 Fragments arranged by size: 2463 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3243 4262 5974 Size: 274 80 33 51 1443 1266 1019 1712 Cuts at: 5974 96 Size: 138 Fragments arranged by size: 1712 1443 1266 1019 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5976 6008 5976 Size: 32 5984 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6016 XbaI T'CTAG_A Cuts at: 3352 3352 Size: 6016 XhoI C'TCGA_G Cuts at: 1943 2157 1943 Size: 214 5802 XmaI C'CCGG_G Cuts at: 1870 2781 3341 1870 Size: 911 560 4545 Fragments arranged by size: 4545 911 560 XmnI GAAnn'nnTTC Cuts at: 4145 4145 Size: 6016 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3824 276 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI