(Circular) (Six-base) MAPSORT of: pFN21AB5018.seq Check: 6256 from: 1 to: 5791 pFN21AB5018 5791 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2359 2728 2359 Size: 369 5422 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2402 3601 278 Size: 53 83 186 936 866 1199 2468 Fragments arranged by size: 2468 1199 936 866 186 83 53 AccI GT'mk_AC Cuts at: 1937 3134 1937 Size: 1197 4594 Acc65I G'GTAC_C Cuts at: 2255 3112 2255 Size: 857 4934 AclI AA'CG_TT Cuts at: 1563 3918 4291 1563 Size: 2355 373 3063 Fragments arranged by size: 3063 2355 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2418 3854 5238 5506 1632 Size: 786 1436 1384 268 1917 Fragments arranged by size: 1917 1436 1384 786 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5594 AflIII A'CryG_T Cuts at: 1203 4690 5667 1203 Size: 3487 977 1327 Fragments arranged by size: 3487 1327 977 AgeI A'CCGG_T Cuts at: 4622 4622 Size: 5791 AhdI GACnn_n'nnGTC Cuts at: 4520 4520 Size: 5791 AleI CACnn'nnGTG Cuts at: 1166 2474 1166 Size: 1308 4483 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2772 1184 Size: 1588 4203 AlwNI CAG_nnn'CTG Cuts at: 2411 2423 2785 2851 3534 5106 2411 Size: 12 362 66 683 1572 3096 Fragments arranged by size: 3096 1572 683 362 66 12 ApaLI G'TGCA_C Cuts at: 3848 5004 3848 Size: 1156 4635 ApoI r'AATT_y Cuts at: 1493 2607 3100 3326 3478 5587 1493 Size: 1114 493 226 152 2109 1697 Fragments arranged by size: 2109 1697 1114 493 226 152 AseI AT'TA_AT Cuts at: 160 1032 4345 160 Size: 872 3313 1606 Fragments arranged by size: 3313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 AvaI C'yCGr_G Cuts at: 1870 1943 2621 3116 1870 Size: 73 678 495 4545 Fragments arranged by size: 4545 678 495 73 BamHI G'GATC_C Cuts at: 3121 3488 3121 Size: 367 5424 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2255 2826 2906 3112 4567 Size: 325 217 627 468 571 80 206 1455 Cuts at: 4567 618 Size: 1842 Fragments arranged by size: 1842 1455 627 571 468 325 217 206 80 BanII G_rGCy'C Cuts at: 1391 2375 2486 2621 2862 3110 1391 Size: 984 111 135 241 248 4072 Fragments arranged by size: 4072 984 248 241 135 111 BbeI G_GCGC'C Cuts at: 2910 2910 Size: 5791 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2463 928 Size: 971 564 4256 Fragments arranged by size: 4256 971 564 BbvCI CC'TCA_GC Cuts at: 2260 2802 2260 Size: 542 5249 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3982 4016 1903 Size: 34 2045 34 3678 Fragments arranged by size: 3678 2045 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3683 4893 3683 Size: 1210 4581 BclI T'GATC_A Cuts at: 1548 1548 Size: 5791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4402 136 Size: 107 122 71 631 605 2730 1525 Fragments arranged by size: 2730 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2163 5786 2163 Size: 3623 2168 BlpI GC'TnA_GC Cuts at: 3208 3208 Size: 5791 Bme1580I G_kGCm'C Cuts at: 1790 3852 5008 1790 Size: 2062 1156 2573 Fragments arranged by size: 2573 2062 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5791 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2713 3522 4480 Size: 416 53 83 399 24 1296 809 958 Cuts at: 4480 442 Size: 1753 Fragments arranged by size: 1753 1296 958 809 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5791 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2717 2762 4451 1239 Size: 1478 45 1689 2579 Fragments arranged by size: 2579 1689 1478 45 Bpu10I CC'TnA_GC Cuts at: 2260 2802 2260 Size: 542 5249 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2188 3916 4781 5079 2188 Size: 1728 865 298 2900 Fragments arranged by size: 2900 1728 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4454 882 Size: 617 2955 2219 Fragments arranged by size: 2955 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3582 493 Size: 711 2378 2702 Fragments arranged by size: 2702 2378 711 BsaBI GATnn'nnATC Cuts at: 5785 5785 Size: 5791 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2399 2907 2951 3598 Size: 53 83 186 936 866 508 44 647 Cuts at: 3598 3980 4615 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 866 647 635 508 382 186 83 53 44 BsaWI w'CCGG_w Cuts at: 4224 4622 4896 5043 5454 4224 Size: 398 274 147 411 4561 Fragments arranged by size: 4561 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2450 2480 2501 2531 1178 Size: 30 165 30 1047 30 21 30 4438 Fragments arranged by size: 4438 1047 165 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2933 1186 Size: 1747 4044 BseYI C'CCAG_C Cuts at: 2785 4994 5490 2785 Size: 2209 496 3086 Fragments arranged by size: 3086 2209 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5218 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3241 4002 5030 1540 Size: 275 186 1240 761 1028 2301 Fragments arranged by size: 2301 1240 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3852 3937 5008 3852 Size: 85 1071 4635 Fragments arranged by size: 4635 1071 85 BsmI GAATG_Cn' Cuts at: 3302 3395 3302 Size: 93 5698 BsmBI CGTCTCn'nnnn_ Cuts at: 2066 2489 2066 Size: 423 5368 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2375 2486 2621 2862 3110 3852 3937 Size: 399 585 111 135 241 248 742 85 Cuts at: 3937 5008 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 585 399 248 241 135 111 85 BspEI T'CCGG_A Cuts at: 5454 5454 Size: 5791 BspHI T'CATG_A Cuts at: 3680 5410 3680 Size: 1730 4061 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2359 2728 3146 844 Size: 1515 369 418 3489 Fragments arranged by size: 3489 1515 418 369 BsrBI CCG'CTC Cuts at: 1394 1823 2539 3678 1394 Size: 429 716 1139 3507 Fragments arranged by size: 3507 1139 716 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4286 4460 65 Size: 1183 3038 174 1396 Fragments arranged by size: 3038 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2156 2246 2668 2823 4435 4622 1700 Size: 110 346 90 422 155 1612 187 2869 Fragments arranged by size: 2869 1612 422 346 187 155 110 90 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5791 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5791 BssSI C'ACGA_G Cuts at: 3851 4863 3851 Size: 1012 4779 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5791 BstBI TT'CG_AA Cuts at: 4595 4595 Size: 5791 BstEII G'GTnAC_C Cuts at: 1184 2230 1184 Size: 1046 4745 BstYI r'GATC_y Cuts at: 1972 2052 2163 3121 3488 3873 3890 5342 5428 Size: 80 111 958 367 385 17 1452 86 Cuts at: 5428 5440 5786 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 958 385 367 346 111 86 80 17 12 Bsu36I CC'TnA_GG Cuts at: 2329 2329 Size: 5791 BtgI C'CryG_G Cuts at: 513 1065 2004 2130 2520 2586 2673 513 Size: 552 939 126 390 66 87 3631 Fragments arranged by size: 3631 939 552 390 126 87 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5052 Fragments arranged by size: 5052 683 56 BtsI GCAGTG_nn' Cuts at: 3307 4101 4121 3307 Size: 794 20 4977 Fragments arranged by size: 4977 794 20 ClaI AT'CG_AT Cuts at: 1554 2202 1554 Size: 648 5143 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5756 DraI TTT'AAA Cuts at: 3095 3095 Size: 5791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4798 809 Size: 507 3482 1802 Fragments arranged by size: 3482 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2248 2547 Size: 54 1425 50 275 18 177 241 299 Cuts at: 2547 2670 3238 4127 5756 8 Size: 123 568 889 1629 43 Fragments arranged by size: 1629 1425 889 568 299 275 241 177 123 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3238 1537 Size: 275 1426 4090 Fragments arranged by size: 4090 1426 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2437 3721 1353 Size: 1084 1284 3423 Fragments arranged by size: 3423 1284 1084 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2377 2891 4378 4752 4898 387 Size: 1064 138 788 514 1487 374 146 1280 Fragments arranged by size: 1487 1280 1064 788 514 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2418 2717 2762 3854 4451 5238 5506 Size: 393 786 299 45 1092 597 787 268 Cuts at: 5506 1239 Size: 1524 Fragments arranged by size: 1524 1092 787 786 597 393 299 268 45 EcoO109I rG'GnC_Cy Cuts at: 2923 2986 2923 Size: 63 5728 EcoRI G'AATT_C Cuts at: 2607 3100 3478 2607 Size: 493 378 4920 Fragments arranged by size: 4920 493 378 FspI TGC'GCA Cuts at: 4297 4297 Size: 5791 HaeII r_GCGC'y Cuts at: 1678 2910 4938 1678 Size: 1232 2028 2531 Fragments arranged by size: 2531 2028 1232 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2130 2162 2399 2431 2450 Size: 32 135 32 558 32 237 32 19 Cuts at: 2450 2482 2501 2526 2533 2558 4432 4464 4506 Size: 32 19 25 7 25 1874 32 42 Cuts at: 4506 4538 1373 Size: 32 2626 Fragments arranged by size: 2626 1874 558 237 135 42 32 32 32 32 32 32 32 25 25 19 19 7 HincII GTy'rAC Cuts at: 669 1657 1938 3135 3381 669 Size: 988 281 1197 246 3079 Fragments arranged by size: 3079 1197 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5791 HpaI GTT'AAC Cuts at: 3381 3381 Size: 5791 KasI G'GCGC_C Cuts at: 2906 2906 Size: 5791 KpnI G_GTAC'C Cuts at: 2259 3116 2259 Size: 857 4934 MfeI C'AATT_G Cuts at: 3390 3390 Size: 5791 MluI A'CGCG_T Cuts at: 5667 5667 Size: 5791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4905 5089 1305 Size: 3600 184 2007 Fragments arranged by size: 3600 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3969 Fragments arranged by size: 3969 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2474 2519 2846 2945 3749 4108 4267 Size: 648 1308 45 327 99 804 359 159 Cuts at: 4267 518 Size: 2042 Fragments arranged by size: 2042 1308 804 648 359 327 159 99 45 MspA1I CmG'CkG Cuts at: 1606 2288 2445 2785 3207 3884 5032 5277 1606 Size: 682 157 340 422 677 1148 245 2120 Fragments arranged by size: 2120 1148 682 677 422 340 245 157 NaeI GCC'GGC Cuts at: 2158 2248 2670 2825 2158 Size: 90 422 155 5124 Fragments arranged by size: 5124 422 155 90 NarI GG'CG_CC Cuts at: 2907 2907 Size: 5791 NcoI C'CATG_G Cuts at: 513 1065 2004 2520 513 Size: 552 939 516 3784 Fragments arranged by size: 3784 939 552 516 NdeI CA'TA_TG Cuts at: 387 387 Size: 5791 NgoMIV G'CCGG_C Cuts at: 2156 2246 2668 2823 2156 Size: 90 422 155 5124 Fragments arranged by size: 5124 422 155 90 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5791 NotI GC'GGCC_GC Cuts at: 3238 3238 Size: 5791 NspI r_CATG'y Cuts at: 1132 1229 2338 2734 3149 4694 1132 Size: 97 1109 396 415 1545 2229 Fragments arranged by size: 2229 1545 1109 415 396 97 PasI CC'CwG_GG Cuts at: 1348 2864 2989 1348 Size: 1516 125 4150 Fragments arranged by size: 4150 1516 125 PciI A'CATG_T Cuts at: 4690 4690 Size: 5791 PflMI CCAn_nnn'nTGG Cuts at: 1108 2785 1108 Size: 1677 4114 PfoI T'CCnGG_A Cuts at: 2643 2643 Size: 5791 PmeI GTTT'AAAC Cuts at: 3095 3095 Size: 5791 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3860 3892 5373 5405 3860 Size: 32 1481 32 4246 Fragments arranged by size: 4246 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5791 PsiI TTA'TAA Cuts at: 3361 3361 Size: 5791 PstI C_TGCA'G Cuts at: 830 2037 3143 830 Size: 1207 1106 3478 Fragments arranged by size: 3478 1207 1106 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5791 PvuII CAG'CTG Cuts at: 2288 2785 2288 Size: 497 5294 SalI G'TCGA_C Cuts at: 1936 3133 1936 Size: 1197 4594 SbfI CC_TGCA'GG Cuts at: 3143 3143 Size: 5791 ScaI AGT'ACT Cuts at: 4039 5751 4039 Size: 1712 4079 SfcI C'TryA_G Cuts at: 826 1045 2033 2970 3139 4274 4955 5146 826 Size: 219 988 937 169 1135 681 191 1471 Fragments arranged by size: 1471 1135 988 937 681 219 191 169 SfoI GGC'GCC Cuts at: 2908 2908 Size: 5791 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5791 SmaI CCC'GGG Cuts at: 1872 3118 1872 Size: 1246 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2167 3895 4796 5058 820 Size: 197 926 224 1728 901 262 1553 Fragments arranged by size: 1728 1553 926 901 262 224 197 SnaBI TAC'GTA Cuts at: 493 3582 493 Size: 3089 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5791 SphI G_CATG'C Cuts at: 1132 3149 1132 Size: 2017 3774 SspI AAT'ATT Cuts at: 5 52 3715 5 Size: 47 3663 2081 Fragments arranged by size: 3663 2081 47 StuI AGG'CCT Cuts at: 1517 2276 3020 1517 Size: 759 744 4288 Fragments arranged by size: 4288 759 744 StyI C'CwwG_G Cuts at: 513 1065 2004 2520 3230 513 Size: 552 939 516 710 3074 Fragments arranged by size: 3074 939 710 552 516 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2296 3818 3835 3988 4173 4618 325 Size: 1255 716 1522 17 153 185 445 1498 Fragments arranged by size: 1522 1498 1255 716 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4037 5749 96 Size: 274 80 33 51 1443 2060 1712 138 Fragments arranged by size: 2060 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2593 2625 5751 5783 2593 Size: 32 3126 32 2601 Fragments arranged by size: 3126 2601 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2177 1316 Size: 861 4930 XbaI T'CTAG_A Cuts at: 3127 3127 Size: 5791 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5791 XmaI C'CCGG_G Cuts at: 1870 3116 1870 Size: 1246 4545 XmnI GAAnn'nnTTC Cuts at: 3920 3920 Size: 5791 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2400 3599 276 Size: 53 83 186 936 866 1199 2468 Fragments arranged by size: 2468 1199 936 866 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI