Restriction map for
pFN21AB5018
(Linear) (Six-base) MAPSORT of: KIBB5018.seq Check: 4828 from: 1 to: 1086
KIBB5018.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 354 723 1086
Size: 354 369 363
Fragments arranged by size:
369 363 354
AatII G_ACGT'C
Cuts at: 0 397 1086
Size: 397 689
Acc65I G'GTAC_C
Cuts at: 0 250 1086
Size: 250 836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 1086
Size: 413 673
AleI CACnn'nnGTG
Cuts at: 0 469 1086
Size: 469 617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 767 1086
Size: 767 319
AlwNI CAG_nnn'CTG
Cuts at: 0 406 418 780 846 1086
Size: 406 12 362 66 240
Fragments arranged by size:
406 362 240 66 12
ApoI r'AATT_y
Cuts at: 0 602 1086
Size: 602 484
AvaI C'yCGr_G
Cuts at: 0 616 1086
Size: 616 470
BanI G'GyrC_C
Cuts at: 0 250 821 901 1086
Size: 250 571 80 185
Fragments arranged by size:
571 250 185 80
BanII G_rGCy'C
Cuts at: 0 370 481 616 857 1086
Size: 370 111 135 241 229
Fragments arranged by size:
370 241 229 135 111
BbeI G_GCGC'C
Cuts at: 0 905 1086
Size: 905 181
BbsI GAAGACnn'nnnn_
Cuts at: 0 458 1086
Size: 458 628
BbvCI CC'TCA_GC
Cuts at: 0 255 797 1086
Size: 255 542 289
Fragments arranged by size:
542 289 255
BglII A'GATC_T
Cuts at: 0 158 1086
Size: 158 928
BmrI ACTGGGnnnn_n'
Cuts at: 0 708 1086
Size: 708 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 757 1086
Size: 712 45 329
Fragments arranged by size:
712 329 45
Bpu10I CC'TnA_GC
Cuts at: 0 255 797 1086
Size: 255 542 289
Fragments arranged by size:
542 289 255
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 1086
Size: 183 903
BsaHI Gr'CG_yC
Cuts at: 0 394 902 946 1086
Size: 394 508 44 140
Fragments arranged by size:
508 394 140 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 445 475 496 526 1086
Size: 445 30 21 30 560
Fragments arranged by size:
560 445 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 928 1086
Size: 928 158
BseYI C'CCAG_C
Cuts at: 0 780 1086
Size: 780 306
BsmBI CGTCTCn'nnnn_
Cuts at: 0 61 484 1086
Size: 61 423 602
Fragments arranged by size:
602 423 61
Bsp1286I G_dGCh'C
Cuts at: 0 370 481 616 857 1086
Size: 370 111 135 241 229
Fragments arranged by size:
370 241 229 135 111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 354 723 1086
Size: 354 369 363
Fragments arranged by size:
369 363 354
BsrBI CCG'CTC
Cuts at: 0 534 1086
Size: 534 552
BsrFI r'CCGG_y
Cuts at: 0 151 241 663 818 1086
Size: 151 90 422 155 268
Fragments arranged by size:
422 268 155 151 90
BstEII G'GTnAC_C
Cuts at: 0 225 1086
Size: 225 861
BstYI r'GATC_y
Cuts at: 0 47 158 1086
Size: 47 111 928
Fragments arranged by size:
928 111 47
Bsu36I CC'TnA_GG
Cuts at: 0 324 1086
Size: 324 762
BtgI C'CryG_G
Cuts at: 0 125 515 581 668 1086
Size: 125 390 66 87 418
Fragments arranged by size:
418 390 125 87 66
ClaI AT'CG_AT
Cuts at: 0 197 1086
Size: 197 889
EaeI y'GGCC_r
Cuts at: 0 2 243 542 665 1086
Size: 2 241 299 123 421
Fragments arranged by size:
421 299 241 123 2
EarI CTCTTCn'nnn_
Cuts at: 0 432 1086
Size: 432 654
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 372 886 1086
Size: 372 514 200
Fragments arranged by size:
514 372 200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 712 757 1086
Size: 413 299 45 329
Fragments arranged by size:
413 329 299 45
EcoO109I rG'GnC_Cy
Cuts at: 0 918 981 1086
Size: 918 63 105
Fragments arranged by size:
918 105 63
EcoRI G'AATT_C
Cuts at: 0 602 1086
Size: 602 484
HaeII r_GCGC'y
Cuts at: 0 905 1086
Size: 905 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 125 157 394 426 445 477 496 521
Size: 125 32 237 32 19 32 19 25
Cuts at: 521 528 553 1086
Size: 7 25 533
Fragments arranged by size:
533 237 125 32 32 32 25 25
19 19 7
KasI G'GCGC_C
Cuts at: 0 901 1086
Size: 901 185
KpnI G_GTAC'C
Cuts at: 0 254 1086
Size: 254 832
MslI CAynn'nnrTG
Cuts at: 0 469 514 841 940 1086
Size: 469 45 327 99 146
Fragments arranged by size:
469 327 146 99 45
MspA1I CmG'CkG
Cuts at: 0 283 440 780 1086
Size: 283 157 340 306
Fragments arranged by size:
340 306 283 157
NaeI GCC'GGC
Cuts at: 0 153 243 665 820 1086
Size: 153 90 422 155 266
Fragments arranged by size:
422 266 155 153 90
NarI GG'CG_CC
Cuts at: 0 902 1086
Size: 902 184
NcoI C'CATG_G
Cuts at: 0 515 1086
Size: 515 571
NgoMIV G'CCGG_C
Cuts at: 0 151 241 663 818 1086
Size: 151 90 422 155 268
Fragments arranged by size:
422 268 155 151 90
NspI r_CATG'y
Cuts at: 0 333 729 1086
Size: 333 396 357
Fragments arranged by size:
396 357 333
PasI CC'CwG_GG
Cuts at: 0 859 984 1086
Size: 859 125 102
Fragments arranged by size:
859 125 102
PflMI CCAn_nnn'nTGG
Cuts at: 0 780 1086
Size: 780 306
PfoI T'CCnGG_A
Cuts at: 0 638 1086
Size: 638 448
PstI C_TGCA'G
Cuts at: 0 32 1086
Size: 32 1054
PvuII CAG'CTG
Cuts at: 0 283 780 1086
Size: 283 497 306
Fragments arranged by size:
497 306 283
SfcI C'TryA_G
Cuts at: 0 28 965 1086
Size: 28 937 121
Fragments arranged by size:
937 121 28
SfoI GGC'GCC
Cuts at: 0 903 1086
Size: 903 183
SmlI C'TyrA_G
Cuts at: 0 162 1086
Size: 162 924
StuI AGG'CCT
Cuts at: 0 271 1015 1086
Size: 271 744 71
Fragments arranged by size:
744 271 71
StyI C'CwwG_G
Cuts at: 0 515 1086
Size: 515 571
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 291 1086
Size: 291 795
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 588 620 1086
Size: 588 32 466
Fragments arranged by size:
588 466 32
Tth111I GACn'n_nGTC
Cuts at: 0 172 1086
Size: 172 914
ZraI GAC'GTC
Cuts at: 0 395 1086
Size: 395 691
Enzymes that do cut:
AarI AatII Acc65I AcuI AleI AlfI AlwNI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BglII BmrI
BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsmBI
Bsp1286I BspMI BsrBI BsrFI BstEII BstYI Bsu36I BtgI
ClaI EaeI EarI EciI Eco57MI EcoO109I EcoRI HaeII
Hin4I KasI KpnI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PstI PvuII SfcI
SfoI SmlI StuI StyI TaqII TstI Tth111I ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI
BmtI BplI BsaI BsaAI BsaBI BsaWI BsgI BsiEI
BsiHKAI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I BtgZI BtsI CspCI
DraI DraIII DrdI EagI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI MfeI MluI
MmeI MscI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI
XcmI XhoI XmaI XmnI