Restriction map for
pFN21AB4976
(Linear) (Six-base) MAPSORT of: KIBB4976.seq Check: 1288 from: 1 to: 1254
KIBB4976.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 0 59 812 1254
Size: 59 753 442
Fragments arranged by size:
753 442 59
Acc65I G'GTAC_C
Cuts at: 0 1225 1254
Size: 1225 29
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 738 1254
Size: 738 516
AlwNI CAG_nnn'CTG
Cuts at: 0 1185 1254
Size: 1185 69
ApaI G_GGCC'C
Cuts at: 0 398 1254
Size: 398 856
ApoI r'AATT_y
Cuts at: 0 456 483 1254
Size: 456 27 771
Fragments arranged by size:
771 456 27
AvaI C'yCGr_G
Cuts at: 0 17 271 989 1254
Size: 17 254 718 265
Fragments arranged by size:
718 265 254 17
BamHI G'GATC_C
Cuts at: 0 129 1254
Size: 129 1125
BanI G'GyrC_C
Cuts at: 0 786 925 1225 1254
Size: 786 139 300 29
Fragments arranged by size:
786 300 139 29
BanII G_rGCy'C
Cuts at: 0 398 1254
Size: 398 856
BbsI GAAGACnn'nnnn_
Cuts at: 0 407 893 1254
Size: 407 486 361
Fragments arranged by size:
486 407 361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 776 810 1254
Size: 776 34 444
Fragments arranged by size:
776 444 34
BglII A'GATC_T
Cuts at: 0 617 695 1008 1254
Size: 617 78 313 246
Fragments arranged by size:
617 313 246 78
Bme1580I G_kGCm'C
Cuts at: 0 398 754 1254
Size: 398 356 500
Fragments arranged by size:
500 398 356
BmgBI CAC'GTC
Cuts at: 0 657 1026 1254
Size: 657 369 228
Fragments arranged by size:
657 369 228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1254
Size: 862 392
BsaI GGTCTCn'nnnn_
Cuts at: 0 547 1254
Size: 547 707
BsaBI GATnn'nnATC
Cuts at: 0 694 1121 1254
Size: 694 427 133
Fragments arranged by size:
694 427 133
BsaHI Gr'CG_yC
Cuts at: 0 1108 1254
Size: 1108 146
BsaWI w'CCGG_w
Cuts at: 0 503 1254
Size: 503 751
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1218 1248 1254
Size: 1218 30 6
Fragments arranged by size:
1218 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 478 1231 1254
Size: 478 753 23
Fragments arranged by size:
753 478 23
BseYI C'CCAG_C
Cuts at: 0 321 539 790 1254
Size: 321 218 251 464
Fragments arranged by size:
464 321 251 218
BsiHKAI G_wGCw'C
Cuts at: 0 332 1254
Size: 332 922
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1099 1254
Size: 1099 155
Bsp1286I G_dGCh'C
Cuts at: 0 332 398 754 1254
Size: 332 66 356 500
Fragments arranged by size:
500 356 332 66
BspEI T'CCGG_A
Cuts at: 0 503 1254
Size: 503 751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 181 1254
Size: 181 1073
BsrBI CCG'CTC
Cuts at: 0 150 1254
Size: 150 1104
BsrFI r'CCGG_y
Cuts at: 0 264 1228 1254
Size: 264 964 26
Fragments arranged by size:
964 264 26
BssSI C'ACGA_G
Cuts at: 0 97 1254
Size: 97 1157
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1026 1254
Size: 1026 228
BstYI r'GATC_y
Cuts at: 0 129 353 470 617 695 938 1008 1254
Size: 129 224 117 147 78 243 70 246
Fragments arranged by size:
246 243 224 147 129 117 78 70
BtgI C'CryG_G
Cuts at: 0 627 761 1031 1254
Size: 627 134 270 223
Fragments arranged by size:
627 270 223 134
BtsI GCAGTG_nn'
Cuts at: 0 382 1254
Size: 382 872
DrdI GACnn_nn'nnGTC
Cuts at: 0 205 433 1254
Size: 205 228 821
Fragments arranged by size:
821 228 205
EaeI y'GGCC_r
Cuts at: 0 189 242 1028 1254
Size: 189 53 786 226
Fragments arranged by size:
786 226 189 53
EarI CTCTTCn'nnn_
Cuts at: 0 761 1208 1254
Size: 761 447 46
Fragments arranged by size:
761 447 46
EcoO109I rG'GnC_Cy
Cuts at: 0 14 198 231 394 855 1254
Size: 14 184 33 163 461 399
Fragments arranged by size:
461 399 184 163 33 14
EcoRI G'AATT_C
Cuts at: 0 483 1254
Size: 483 771
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 93 1254
Size: 93 1161
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 623 655 1000 1032 1254
Size: 623 32 345 32 222
Fragments arranged by size:
623 345 222 32 32
HincII GTy'rAC
Cuts at: 0 648 1254
Size: 648 606
KpnI G_GTAC'C
Cuts at: 0 1229 1254
Size: 1229 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 1254
Size: 557 697
MscI TGG'CCA
Cuts at: 0 244 1254
Size: 244 1010
MslI CAynn'nnrTG
Cuts at: 0 1051 1254
Size: 1051 203
NcoI C'CATG_G
Cuts at: 0 627 761 1254
Size: 627 134 493
Fragments arranged by size:
627 493 134
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 886 918 1254
Size: 886 32 336
Fragments arranged by size:
886 336 32
PpuMI rG'GwC_Cy
Cuts at: 0 14 198 231 1254
Size: 14 184 33 1023
Fragments arranged by size:
1023 184 33 14
PspOMI G'GGCC_C
Cuts at: 0 394 1254
Size: 394 860
PstI C_TGCA'G
Cuts at: 0 302 389 1254
Size: 302 87 865
Fragments arranged by size:
865 302 87
SanDI GG'GwC_CC
Cuts at: 0 14 1254
Size: 14 1240
SfcI C'TryA_G
Cuts at: 0 298 385 1254
Size: 298 87 869
Fragments arranged by size:
869 298 87
SmlI C'TyrA_G
Cuts at: 0 877 1254
Size: 877 377
StuI AGG'CCT
Cuts at: 0 531 1254
Size: 531 723
StyI C'CwwG_G
Cuts at: 0 627 761 863 1254
Size: 627 134 102 391
Fragments arranged by size:
627 391 134 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 804 1254
Size: 804 450
TatI w'GTAC_w
Cuts at: 0 743 1254
Size: 743 511
Enzymes that do cut:
AccI Acc65I AlfI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BcgI BglII Bme1580I BmgBI BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstXI
BstYI BtgI BtsI DrdI EaeI EarI EcoO109I EcoRI
FalI Hin4I HincII KpnI MmeI MscI MslI NcoI
PpiI PpuMI PspOMI PstI SanDI SfcI SmlI StuI
StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BciVI BclI BfrBI BglI BlpI
BmrI BmtI BplI BpmI Bpu10I BsaAI BsgI BsiEI
BsiWI BsmI BspHI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EagI EciI EcoICRI Eco57MI EcoNI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI MfeI MluI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI