(Circular) (Six-base) MAPSORT of: pFN21AB4735.seq Check: 5705 from: 1 to: 5857 pFN21AB4735 5857 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3667 278 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3200 1937 Size: 1263 4594 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3156 3984 4357 1563 Size: 1593 828 373 3063 Fragments arranged by size: 3063 1593 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2002 2854 3920 5304 5572 1632 Size: 370 852 1066 1384 268 1917 Fragments arranged by size: 1917 1384 1066 852 370 268 AflII C'TTAA_G Cuts at: 820 1017 2189 820 Size: 197 1172 4488 Fragments arranged by size: 4488 1172 197 AflIII A'CryG_T Cuts at: 1203 2914 4756 5733 1203 Size: 1711 1842 977 1327 Fragments arranged by size: 1842 1711 1327 977 AgeI A'CCGG_T Cuts at: 4688 4688 Size: 5857 AhdI GACnn_n'nnGTC Cuts at: 4586 4586 Size: 5857 AleI CACnn'nnGTG Cuts at: 1166 3053 1166 Size: 1887 3970 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2800 1184 Size: 1616 4241 AlwNI CAG_nnn'CTG Cuts at: 3600 5172 3600 Size: 1572 4285 ApaLI G'TGCA_C Cuts at: 3914 5070 3914 Size: 1156 4701 ApoI r'AATT_y Cuts at: 1493 2471 2894 3006 3029 3166 3392 3544 5653 Size: 978 423 112 23 137 226 152 2109 Cuts at: 5653 1493 Size: 1697 Fragments arranged by size: 2109 1697 978 423 226 152 137 112 23 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 3182 1870 Size: 73 1239 4545 Fragments arranged by size: 4545 1239 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2177 2210 2177 Size: 33 5824 BamHI G'GATC_C Cuts at: 2596 3187 3554 2596 Size: 591 367 4899 Fragments arranged by size: 4899 591 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3178 4633 618 Size: 325 217 627 1391 1455 1842 Fragments arranged by size: 1842 1455 1391 627 325 217 BanII G_rGCy'C Cuts at: 1391 2291 2864 3176 1391 Size: 900 573 312 4072 Fragments arranged by size: 4072 900 573 312 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4048 4082 1903 Size: 34 2111 34 3678 Fragments arranged by size: 3678 2111 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 2953 1548 Size: 1405 4452 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4468 136 Size: 107 122 71 631 605 2796 1525 Fragments arranged by size: 2796 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2160 5852 2160 Size: 3692 2165 BlpI GC'TnA_GC Cuts at: 3274 3274 Size: 5857 Bme1580I G_kGCm'C Cuts at: 1790 2881 3918 5074 1790 Size: 1091 1037 1156 2573 Fragments arranged by size: 2573 1156 1091 1037 BmgBI CAC'GTC Cuts at: 1318 2797 1318 Size: 1479 4378 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3588 4546 442 Size: 416 53 83 399 24 2171 958 1753 Fragments arranged by size: 2171 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2305 2878 3125 4517 1239 Size: 1066 573 247 1392 2579 Fragments arranged by size: 2579 1392 1066 573 247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2122 3982 4847 5145 2122 Size: 1860 865 298 2834 Fragments arranged by size: 2834 1860 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4520 882 Size: 617 3021 2219 Fragments arranged by size: 3021 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2917 3648 493 Size: 711 1713 731 2702 Fragments arranged by size: 2702 1713 731 711 BsaBI GATnn'nnATC Cuts at: 5851 5851 Size: 5857 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3664 4046 4681 275 Size: 53 83 186 936 2131 382 635 1451 Fragments arranged by size: 2131 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4290 4688 4962 5109 5520 4290 Size: 398 274 147 411 4627 Fragments arranged by size: 4627 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2039 2054 2069 1178 Size: 30 165 30 621 15 15 15 4966 Fragments arranged by size: 4966 621 165 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5857 BseYI C'CCAG_C Cuts at: 2569 2996 3042 5060 5556 2569 Size: 427 46 2018 496 2870 Fragments arranged by size: 2870 2018 496 427 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2278 2543 1163 Size: 573 542 265 4477 Fragments arranged by size: 4477 573 542 265 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3307 4068 5096 1540 Size: 275 186 1306 761 1028 2301 Fragments arranged by size: 2301 1306 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3105 3918 4003 5074 3105 Size: 813 85 1071 3888 Fragments arranged by size: 3888 1071 813 85 BsmI GAATG_Cn' Cuts at: 3368 3461 3368 Size: 93 5764 BsmBI CGTCTCn'nnnn_ Cuts at: 2424 2424 Size: 5857 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2291 2864 2881 3105 3176 3918 4003 Size: 399 501 573 17 224 71 742 85 Cuts at: 4003 5074 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 573 501 399 224 85 71 17 BspEI T'CCGG_A Cuts at: 5520 5520 Size: 5857 BspHI T'CATG_A Cuts at: 2457 3746 5476 2457 Size: 1289 1730 2838 Fragments arranged by size: 2838 1730 1289 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2852 3212 844 Size: 2008 360 3489 Fragments arranged by size: 3489 2008 360 BsrBI CCG'CTC Cuts at: 1394 1823 3744 1394 Size: 429 1921 3507 Fragments arranged by size: 3507 1921 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4352 4526 65 Size: 1183 3104 174 1396 Fragments arranged by size: 3104 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4501 4688 1700 Size: 110 2691 187 2869 Fragments arranged by size: 2869 2691 187 110 BsrGI T'GTAC_A Cuts at: 96 2303 2499 96 Size: 2207 196 3454 Fragments arranged by size: 3454 2207 196 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5857 BssSI C'ACGA_G Cuts at: 3917 4929 3917 Size: 1012 4845 BstAPI GCAn_nnn'nTGC Cuts at: 794 2566 794 Size: 1772 4085 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 2266 2344 2839 1184 Size: 1082 78 495 4202 Fragments arranged by size: 4202 1082 495 78 BstXI CCAn_nnnn'nTGG Cuts at: 2326 2326 Size: 5857 BstYI r'GATC_y Cuts at: 1972 2160 2596 3187 3554 3939 3956 5408 5494 Size: 188 436 591 367 385 17 1452 86 Cuts at: 5494 5506 5852 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 591 436 385 367 346 188 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5305 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5118 Fragments arranged by size: 5118 683 56 BtsI GCAGTG_nn' Cuts at: 2934 3037 3373 4167 4187 2934 Size: 103 336 794 20 4604 Fragments arranged by size: 4604 794 336 103 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 3161 3161 Size: 5857 DraIII CAC_nnn'GTG Cuts at: 2301 2301 Size: 5857 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2699 4864 809 Size: 507 1383 2165 1802 Fragments arranged by size: 2165 1802 1383 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2316 2328 2988 Size: 54 1425 50 275 18 486 12 660 Cuts at: 2988 3304 4193 5822 8 Size: 316 889 1629 43 Fragments arranged by size: 1629 1425 889 660 486 316 275 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 3304 1537 Size: 275 1492 4090 Fragments arranged by size: 4090 1492 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3787 1353 Size: 2434 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4444 4818 4964 387 Size: 1064 138 2855 374 146 1280 Fragments arranged by size: 2855 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2002 2305 2854 2878 3125 3920 4517 Size: 393 370 303 549 24 247 795 597 Cuts at: 4517 5304 5572 1239 Size: 787 268 1524 Fragments arranged by size: 1524 795 787 597 549 393 370 303 268 247 24 EcoNI CCTnn'n_nnAGG Cuts at: 2330 2825 2990 2330 Size: 495 165 5197 Fragments arranged by size: 5197 495 165 EcoO109I rG'GnC_Cy Cuts at: 2132 2142 2335 2386 2701 2132 Size: 10 193 51 315 5288 Fragments arranged by size: 5288 315 193 51 10 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2032 2163 2032 Size: 131 5726 FspI TGC'GCA Cuts at: 2981 4363 2981 Size: 1382 4475 HaeII r_GCGC'y Cuts at: 1678 2408 5004 1678 Size: 730 2596 2531 Fragments arranged by size: 2596 2531 730 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 4498 4530 4572 Size: 32 135 32 467 32 2427 32 42 Cuts at: 4572 4604 1373 Size: 32 2626 Fragments arranged by size: 2626 2427 467 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3201 3447 669 Size: 988 281 1263 246 3079 Fragments arranged by size: 3079 1263 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 3456 3456 Size: 5857 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2072 2465 4971 5155 1305 Size: 767 393 2506 184 2007 Fragments arranged by size: 2506 2007 767 393 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2318 10 Size: 54 1425 343 486 3549 Fragments arranged by size: 3549 1425 486 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3053 3815 4174 4333 518 Size: 648 1887 762 359 159 2042 Fragments arranged by size: 2042 1887 762 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2874 3273 3950 5098 5343 1606 Size: 1268 399 677 1148 245 2120 Fragments arranged by size: 2120 1268 1148 677 399 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5305 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NspI r_CATG'y Cuts at: 1132 1229 2686 3215 4760 1132 Size: 97 1457 529 1545 2229 Fragments arranged by size: 2229 1545 1457 529 97 PasI CC'CwG_GG Cuts at: 1348 3084 1348 Size: 1736 4121 PciI A'CATG_T Cuts at: 4756 4756 Size: 5857 PflMI CCAn_nnn'nTGG Cuts at: 1108 2887 1108 Size: 1779 4078 PfoI T'CCnGG_A Cuts at: 2282 2790 2855 2282 Size: 508 65 5284 Fragments arranged by size: 5284 508 65 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PmlI CAC'GTG Cuts at: 2917 2917 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2132 2335 2386 2701 2132 Size: 203 51 315 5288 Fragments arranged by size: 5288 315 203 51 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 2070 3427 2070 Size: 1357 4500 PstI C_TGCA'G Cuts at: 830 3209 830 Size: 2379 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 PvuII CAG'CTG Cuts at: 2874 2874 Size: 5857 RsrII CG'GwC_CG Cuts at: 2435 2435 Size: 5857 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SanDI GG'GwC_CC Cuts at: 2132 2386 2132 Size: 254 5603 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 4105 5817 4105 Size: 1712 4145 SexAI A'CCwGG_T Cuts at: 2270 2270 Size: 5857 SfcI C'TryA_G Cuts at: 826 1045 2833 3205 4340 5021 5212 826 Size: 219 1788 372 1135 681 191 1471 Fragments arranged by size: 1788 1471 1135 681 372 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5857 SmaI CCC'GGG Cuts at: 1872 3184 1872 Size: 1312 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2137 2189 3961 4862 5124 820 Size: 197 926 194 52 1772 901 262 1553 Fragments arranged by size: 1772 1553 926 901 262 197 194 52 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 2686 3215 1132 Size: 1554 529 3774 Fragments arranged by size: 3774 1554 529 SspI AAT'ATT Cuts at: 5 52 2546 3781 5 Size: 47 2494 1235 2081 Fragments arranged by size: 2494 2081 1235 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5857 StyI C'CwwG_G Cuts at: 513 1065 2525 2633 3296 513 Size: 552 1460 108 663 3074 Fragments arranged by size: 3074 1460 663 552 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2214 2617 3884 3901 4054 4239 4684 Size: 1255 634 403 1267 17 153 185 445 Cuts at: 4684 325 Size: 1498 Fragments arranged by size: 1498 1267 1255 634 445 403 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2303 2499 4103 Size: 274 80 33 51 1443 326 196 1604 Cuts at: 4103 5815 96 Size: 1712 138 Fragments arranged by size: 1712 1604 1443 326 274 196 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5817 5849 5817 Size: 32 5825 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 3193 3193 Size: 5857 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3086 3116 3086 Size: 30 5827 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5857 XmaI C'CCGG_G Cuts at: 1870 3182 1870 Size: 1312 4545 XmnI GAAnn'nnTTC Cuts at: 3986 3986 Size: 5857 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3665 276 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI SacI SacII SapI SfiI SfoI SrfI SwaI