Restriction map for
pFN21AB4735
(Linear) (Six-base) MAPSORT of: KIBB4735.seq Check: 2630 from: 1 to: 1152
KIBB4735.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 849 1152
Size: 849 303
AflII C'TTAA_G
Cuts at: 0 184 1152
Size: 184 968
AflIII A'CryG_T
Cuts at: 0 909 1152
Size: 909 243
AleI CACnn'nnGTG
Cuts at: 0 1048 1152
Size: 1048 104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 795 1152
Size: 795 357
ApoI r'AATT_y
Cuts at: 0 466 889 1001 1024 1152
Size: 466 423 112 23 128
Fragments arranged by size:
466 423 128 112 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 172 205 1152
Size: 172 33 947
Fragments arranged by size:
947 172 33
BamHI G'GATC_C
Cuts at: 0 591 1152
Size: 591 561
BanII G_rGCy'C
Cuts at: 0 286 859 1152
Size: 286 573 293
Fragments arranged by size:
573 293 286
BclI T'GATC_A
Cuts at: 0 948 1152
Size: 948 204
BglII A'GATC_T
Cuts at: 0 155 1152
Size: 155 997
Bme1580I G_kGCm'C
Cuts at: 0 876 1152
Size: 876 276
BmgBI CAC'GTC
Cuts at: 0 792 1152
Size: 792 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 873 1120 1152
Size: 300 573 247 32
Fragments arranged by size:
573 300 247 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 1152
Size: 117 1035
BsaAI yAC'GTr
Cuts at: 0 912 1152
Size: 912 240
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 34 49 64 1152
Size: 19 15 15 15 1088
Fragments arranged by size:
1088 19 15 15 15
BseYI C'CCAG_C
Cuts at: 0 564 991 1037 1152
Size: 564 427 46 115
Fragments arranged by size:
564 427 115 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 538 1152
Size: 273 265 614
Fragments arranged by size:
614 273 265
BsiHKAI G_wGCw'C
Cuts at: 0 1100 1152
Size: 1100 52
BsmBI CGTCTCn'nnnn_
Cuts at: 0 419 1152
Size: 419 733
Bsp1286I G_dGCh'C
Cuts at: 0 286 859 876 1100 1152
Size: 286 573 17 224 52
Fragments arranged by size:
573 286 224 52 17
BspHI T'CATG_A
Cuts at: 0 452 1152
Size: 452 700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 847 1152
Size: 847 305
BsrGI T'GTAC_A
Cuts at: 0 298 494 1152
Size: 298 196 658
Fragments arranged by size:
658 298 196
BstAPI GCAn_nnn'nTGC
Cuts at: 0 561 1152
Size: 561 591
BstEII G'GTnAC_C
Cuts at: 0 261 339 834 1152
Size: 261 78 495 318
Fragments arranged by size:
495 318 261 78
BstXI CCAn_nnnn'nTGG
Cuts at: 0 321 1152
Size: 321 831
BstYI r'GATC_y
Cuts at: 0 155 591 1152
Size: 155 436 561
Fragments arranged by size:
561 436 155
BtsI GCAGTG_nn'
Cuts at: 0 929 1032 1152
Size: 929 103 120
Fragments arranged by size:
929 120 103
DraIII CAC_nnn'GTG
Cuts at: 0 296 1152
Size: 296 856
DrdI GACnn_nn'nnGTC
Cuts at: 0 694 1152
Size: 694 458
EaeI y'GGCC_r
Cuts at: 0 311 323 983 1152
Size: 311 12 660 169
Fragments arranged by size:
660 311 169 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 849 873 1120 1152
Size: 300 549 24 247 32
Fragments arranged by size:
549 300 247 32 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 820 985 1152
Size: 325 495 165 167
Fragments arranged by size:
495 325 167 165
EcoO109I rG'GnC_Cy
Cuts at: 0 127 137 330 381 696 1152
Size: 127 10 193 51 315 456
Fragments arranged by size:
456 315 193 127 51 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 27 158 1152
Size: 27 131 994
Fragments arranged by size:
994 131 27
FspI TGC'GCA
Cuts at: 0 976 1152
Size: 976 176
HaeII r_GCGC'y
Cuts at: 0 403 1152
Size: 403 749
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 1152
Size: 34 32 1086
Fragments arranged by size:
1086 34 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 460 1152
Size: 67 393 692
Fragments arranged by size:
692 393 67
MscI TGG'CCA
Cuts at: 0 313 1152
Size: 313 839
MslI CAynn'nnrTG
Cuts at: 0 1048 1152
Size: 1048 104
MspA1I CmG'CkG
Cuts at: 0 869 1152
Size: 869 283
NspI r_CATG'y
Cuts at: 0 681 1152
Size: 681 471
PasI CC'CwG_GG
Cuts at: 0 1079 1152
Size: 1079 73
PflMI CCAn_nnn'nTGG
Cuts at: 0 882 1152
Size: 882 270
PfoI T'CCnGG_A
Cuts at: 0 277 785 850 1152
Size: 277 508 65 302
Fragments arranged by size:
508 302 277 65
PmlI CAC'GTG
Cuts at: 0 912 1152
Size: 912 240
PpuMI rG'GwC_Cy
Cuts at: 0 127 330 381 696 1152
Size: 127 203 51 315 456
Fragments arranged by size:
456 315 203 127 51
PsiI TTA'TAA
Cuts at: 0 65 1152
Size: 65 1087
PvuII CAG'CTG
Cuts at: 0 869 1152
Size: 869 283
RsrII CG'GwC_CG
Cuts at: 0 430 1152
Size: 430 722
SanDI GG'GwC_CC
Cuts at: 0 127 381 1152
Size: 127 254 771
Fragments arranged by size:
771 254 127
SexAI A'CCwGG_T
Cuts at: 0 265 1152
Size: 265 887
SfcI C'TryA_G
Cuts at: 0 828 1152
Size: 828 324
SmlI C'TyrA_G
Cuts at: 0 132 184 1152
Size: 132 52 968
Fragments arranged by size:
968 132 52
SphI G_CATG'C
Cuts at: 0 681 1152
Size: 681 471
SspI AAT'ATT
Cuts at: 0 541 1152
Size: 541 611
StyI C'CwwG_G
Cuts at: 0 520 628 1152
Size: 520 108 524
Fragments arranged by size:
524 520 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 209 612 1152
Size: 209 403 540
Fragments arranged by size:
540 403 209
TatI w'GTAC_w
Cuts at: 0 298 494 1152
Size: 298 196 658
Fragments arranged by size:
658 298 196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1081 1111 1152
Size: 1081 30 41
Fragments arranged by size:
1081 41 30
Enzymes that do cut:
AcuI AflII AflIII AleI AlfI ApoI BaeI BamHI
BanII BclI BglII Bme1580I BmgBI BpmI BpuEI BsaAI
BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrGI BstAPI BstEII BstXI BstYI BtsI DraIII DrdI
EaeI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
MmeI MscI MslI MspA1I NspI PasI PflMI PfoI
PmlI PpuMI PsiI PvuII RsrII SanDI SexAI SfcI
SmlI SphI SspI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BanI BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BlpI BmrI BmtI BplI Bpu10I BsaI BsaBI
BsaHI BsaWI BseRI BsiEI BsiWI BsmI BspEI BsrBI
BsrDI BsrFI BssHII BssSI BstBI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI EagI EarI EciI EcoICRI
EcoRI EcoRV FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PpiI
PshAI PspOMI PspXI PsrI PstI PvuI SacI SacII
SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI