Restriction map for
pFN21AB4690
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# NO CUT ENZYMES ( 98 enzymes) #
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AatII AccI AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspI AvrII
BbrPI BcgI BclI BfaI BfrI
BlnI BsaAI BsaBI BseAI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI Eco47III
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KspI MaeI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1991 GGTACC G^GTACC
AccII 139 CGCG CG^CG
AhdI 940 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1991 GGTACC G^GTACC
AspEI 940 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 644 TGCGCA TGC^GCA
BalI 2513 TGGCCA TGG^CCA
BamHI 2505 GGATCC G^GATCC
BciVI 1932 GTATCC GTATCC(6/5)
BglII 1821 AGATCT A^GATCT
BsaI 2275 GAGACC GGTCTC(1/5)
BsaWI 971 ACCGGA [AT]^CCGG[AT]
BsiEI 199 CGGCCG CG[GA][CT]^CG
BspLU11I 2420 ACATGT A^CATGT
BspMI 1529 ACCTGC ACCTGC(4/8)
BsrDI 464 CATTGC GCAATG(2/0)
BsrFI 201 GCCGGT [GA]^CCGG[CT]
BssSI 737 CTCGTG CACGAG(-5/-1)
BstUI 139 CGCG CG^CG
Cfr10I 201 GCCGGT [GA]^CCGG[CT]
DraIII 1432 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1641 GACCATGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 199 CGGCCG C^GGCCG
Eam1105I 940 GACACAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 199 CGGCCG C^GGCCG
Eco52I 199 CGGCCG C^GGCCG
EcoNI 331 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 644 TGCGCA TGC^GCA
HgaI 922 GCGTC GACGC(5/10)
KpnI 1991 GGTACC GGTAC^C
MaeII 2054 ACGT A^CGT
MluNI 2513 TGGCCA TGG^CCA
MscI 2513 TGGCCA TGG^CCA
MvnI 139 CGCG CG^CG
NruI 138 TCGCGA TCG^CGA
PaeR7I 662 CTCGAG C^TCGAG
SapI 2346 GCTCTTC GCTCTTC(1/4)
SfiI 682 GGCCCAGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 339 AGGCCT AGG^CCT
XcmI 2640 CCAGGACAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 662 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcyI 73 GGCGCC G[GA]^CG[CT]C
2484 GGCGCC
AfaI 1770 GTAC GT^AC
1992 GTAC
AflIII 2054 ACGTGT A^C[GA][CT]GT
2420 ACATGT
Asp700I 1371 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1782 GAAGGAGTTC
AvaI 16 CCCGAG C^[CT]CG[GA]G
662 CTCGAG
BbeI 73 GGCGCC GGCGC^C
2484 GGCGCC
BbsI 1638 GAAGAC GAAGAC(2/6)
2185 GAAGAC
BbvCI 2341 CCTCAGC CCTCAGC(-5/-2)
2295 GCTGAGG
BpuAI 1638 GAAGAC GAAGAC(2/6)
2185 GAAGAC
BsaHI 73 GGCGCC G[GA]^CG[CT]C
2484 GGCGCC
BsoBI 16 CCCGAG C^[CT]CG[GA]G
662 CTCGAG
BstXI 1501 CCACGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1995 CCATGCTGGTGG
EaeI 199 CGGCCG [CT]^GGCC[GA]
2513 TGGCCA
EcoRI 266 GAATTC G^AATTC
861 GAATTC
Hin1I 73 GGCGCC G[GA]^CG[CT]C
2484 GGCGCC
HphI 406 GGTGA GGTGA(8/7)
1467 TCACC
KasI 73 GGCGCC G^GCGCC
2484 GGCGCC
NarI 73 GGCGCC GG^CGCC
2484 GGCGCC
NspI 2420 ACATGT [GA]CATG^[CT]
2538 GCATGT
PflMI 337 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
810 CCAGGCTTTGG
PleI 293 GACTC GAGTC(4/5)
1057 GACTC
RsaI 1770 GTAC GT^AC
1992 GTAC
SbfI 1530 CCTGCAGG CCTGCA^GG
1617 CCTGCAGG
SfoI 73 GGCGCC GGC^GCC
2484 GGCGCC
Sse8387I 1530 CCTGCAGG CCTGCA^GG
1617 CCTGCAGG
Van91I 337 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
810 CCAGGCTTTGG
XmnI 1371 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1782 GAAGGAGTTC
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApaI ApoI AspHI AvaII BanI
BanII BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PpuMI PstI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII