(Linear) (Six-base) MAPSORT of: KIBB4690.seq Check: 6133 from: 1 to: 2685 KIBB4690.orf With 182 enzymes: SgfI * September 18, 2009 17:00 .. Acc65I G'GTAC_C Cuts at: 0 1991 2685 Size: 1991 694 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 460 535 856 1139 1822 2510 2562 Size: 79 381 75 321 283 683 688 52 Cuts at: 2562 2685 Size: 123 Fragments arranged by size: 688 683 381 321 283 123 79 75 52 AflIII A'CryG_T Cuts at: 0 2054 2420 2685 Size: 2054 366 265 Fragments arranged by size: 2054 366 265 AhdI GACnn_n'nnGTC Cuts at: 0 945 2685 Size: 945 1740 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 602 1546 2685 Size: 602 944 1139 Fragments arranged by size: 1139 944 602 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 710 742 2685 Size: 710 32 1943 Fragments arranged by size: 1943 710 32 AlwNI CAG_nnn'CTG Cuts at: 0 931 1093 1710 2263 2380 2685 Size: 931 162 617 553 117 305 Fragments arranged by size: 931 617 553 305 162 117 ApaI G_GGCC'C Cuts at: 0 386 685 694 799 2449 2685 Size: 386 299 9 105 1650 236 Fragments arranged by size: 1650 386 299 236 105 9 ApoI r'AATT_y Cuts at: 0 266 375 861 1130 2077 2685 Size: 266 109 486 269 947 608 Fragments arranged by size: 947 608 486 269 266 109 AvaI C'yCGr_G Cuts at: 0 16 662 2685 Size: 16 646 2023 Fragments arranged by size: 2023 646 16 BamHI G'GATC_C Cuts at: 0 2505 2685 Size: 2505 180 BanI G'GyrC_C Cuts at: 0 73 598 1991 2484 2685 Size: 73 525 1393 493 201 Fragments arranged by size: 1393 525 493 201 73 BanII G_rGCy'C Cuts at: 0 123 386 530 685 694 723 799 1039 Size: 123 263 144 155 9 29 76 240 Cuts at: 1039 1142 2449 2685 Size: 103 1307 236 Fragments arranged by size: 1307 263 240 236 155 144 123 103 76 29 9 BbeI G_GCGC'C Cuts at: 0 77 2488 2685 Size: 77 2411 197 Fragments arranged by size: 2411 197 77 BbsI GAAGACnn'nnnn_ Cuts at: 0 1645 2192 2685 Size: 1645 547 493 Fragments arranged by size: 1645 547 493 BbvCI CC'TCA_GC Cuts at: 0 2296 2342 2685 Size: 2296 46 343 Fragments arranged by size: 2296 343 46 BciVI GTATCCnnnnn_n' Cuts at: 0 1943 2685 Size: 1943 742 BglI GCCn_nnn'nGGC Cuts at: 0 71 106 182 689 2444 2654 2685 Size: 71 35 76 507 1755 210 31 Fragments arranged by size: 1755 507 210 76 71 35 31 BglII A'GATC_T Cuts at: 0 1821 2685 Size: 1821 864 BlpI GC'TnA_GC Cuts at: 0 449 2594 2633 2685 Size: 449 2145 39 52 Fragments arranged by size: 2145 449 52 39 Bme1580I G_kGCm'C Cuts at: 0 386 601 685 694 799 2449 2685 Size: 386 215 84 9 105 1650 236 Fragments arranged by size: 1650 386 236 215 105 84 9 BmgBI CAC'GTC Cuts at: 0 2055 2685 Size: 2055 630 BmrI ACTGGGnnnn_n' Cuts at: 0 598 679 2042 2685 Size: 598 81 1363 643 Fragments arranged by size: 1363 643 598 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1074 2685 Size: 1074 1611 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 44 583 659 779 1258 2003 2264 2685 Size: 44 539 76 120 479 745 261 421 Fragments arranged by size: 745 539 479 421 261 120 76 44 Bpu10I CC'TnA_GC Cuts at: 0 1454 1837 2296 2342 2456 2685 Size: 1454 383 459 46 114 229 Fragments arranged by size: 1454 459 383 229 114 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1378 1456 2543 2685 Size: 1378 78 1087 142 Fragments arranged by size: 1378 1087 142 78 BsaI GGTCTCn'nnnn_ Cuts at: 0 2269 2685 Size: 2269 416 BsaHI Gr'CG_yC Cuts at: 0 74 2485 2685 Size: 74 2411 200 Fragments arranged by size: 2411 200 74 BsaWI w'CCGG_w Cuts at: 0 971 2685 Size: 971 1714 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 710 740 2665 2685 Size: 710 30 1925 20 Fragments arranged by size: 1925 710 30 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 157 302 383 442 445 496 641 949 Size: 157 145 81 59 3 51 145 308 Cuts at: 949 1291 2329 2548 2685 Size: 342 1038 219 137 Fragments arranged by size: 1038 342 308 219 157 145 145 137 81 59 51 3 BseYI C'CCAG_C Cuts at: 0 1007 1086 2006 2685 Size: 1007 79 920 679 Fragments arranged by size: 1007 920 679 79 BsiEI CG_ry'CG Cuts at: 0 202 2685 Size: 202 2483 BsiHKAI G_wGCw'C Cuts at: 0 123 530 1039 1283 2600 2685 Size: 123 407 509 244 1317 85 Fragments arranged by size: 1317 509 407 244 123 85 Bsp1286I G_dGCh'C Cuts at: 0 123 386 530 601 685 694 723 799 Size: 123 263 144 71 84 9 29 76 Cuts at: 799 1039 1142 1283 2449 2600 2685 Size: 240 103 141 1166 151 85 Fragments arranged by size: 1166 263 240 151 144 141 123 103 85 84 76 71 29 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1538 2685 Size: 1538 1147 BsrBI CCG'CTC Cuts at: 0 156 235 435 2043 2685 Size: 156 79 200 1608 642 Fragments arranged by size: 1608 642 200 156 79 BsrDI GCAATG_nn' Cuts at: 0 463 2685 Size: 463 2222 BsrFI r'CCGG_y Cuts at: 0 201 2685 Size: 201 2484 BssSI C'ACGA_G Cuts at: 0 737 2685 Size: 737 1948 BstXI CCAn_nnnn'nTGG Cuts at: 0 1508 2002 2685 Size: 1508 494 683 Fragments arranged by size: 1508 683 494 BstYI r'GATC_y Cuts at: 0 803 824 1163 1821 2505 2685 Size: 803 21 339 658 684 180 Fragments arranged by size: 803 684 658 339 180 21 BtgI C'CryG_G Cuts at: 0 713 1071 1758 1891 2426 2649 2685 Size: 713 358 687 133 535 223 36 Fragments arranged by size: 713 687 535 358 223 133 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2335 2685 Size: 2335 350 BtsI GCAGTG_nn' Cuts at: 0 1311 2685 Size: 1311 1374 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 269 304 359 394 2685 Size: 269 35 55 35 2291 Fragments arranged by size: 2291 269 55 35 35 DraIII CAC_nnn'GTG Cuts at: 0 1437 2685 Size: 1437 1248 DrdI GACnn_nn'nnGTC Cuts at: 0 1647 2685 Size: 1647 1038 EaeI y'GGCC_r Cuts at: 0 199 2513 2685 Size: 199 2314 172 Fragments arranged by size: 2314 199 172 EagI C'GGCC_G Cuts at: 0 199 2685 Size: 199 2486 EarI CTCTTCn'nnn_ Cuts at: 0 848 872 1268 2096 2156 2162 2353 2362 Size: 848 24 396 828 60 6 191 9 Cuts at: 2362 2486 2685 Size: 124 199 Fragments arranged by size: 848 828 396 199 191 124 60 24 9 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 305 2685 Size: 305 2380 EcoICRI GAG'CTC Cuts at: 0 121 528 1037 2685 Size: 121 407 509 1648 Fragments arranged by size: 1648 509 407 121 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 44 79 460 535 583 659 779 856 Size: 44 35 381 75 48 76 120 77 Cuts at: 856 1139 1258 1822 2003 2264 2510 2562 2685 Size: 283 119 564 181 261 246 52 123 Fragments arranged by size: 564 381 283 261 246 181 123 120 119 77 76 75 52 48 44 35 EcoNI CCTnn'n_nnAGG Cuts at: 0 335 2685 Size: 335 2350 EcoO109I rG'GnC_Cy Cuts at: 0 382 383 658 691 796 1449 1614 1764 Size: 382 1 275 33 105 653 165 150 Cuts at: 1764 1881 2445 2685 Size: 117 564 240 Fragments arranged by size: 653 564 382 275 240 165 150 117 105 33 1 EcoRI G'AATT_C Cuts at: 0 266 861 2685 Size: 266 595 1824 Fragments arranged by size: 1824 595 266 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1182 1578 2592 2685 Size: 1182 396 1014 93 Fragments arranged by size: 1182 1014 396 93 FspI TGC'GCA Cuts at: 0 646 2685 Size: 646 2039 FspAI rTGC'GCAy Cuts at: 0 646 2685 Size: 646 2039 HaeII r_GCGC'y Cuts at: 0 77 2482 2488 2685 Size: 77 2405 6 197 Fragments arranged by size: 2405 197 77 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 29 105 110 137 142 772 804 849 Size: 29 76 5 27 5 630 32 45 Cuts at: 849 881 886 918 931 963 2134 2166 2685 Size: 32 5 32 13 32 1171 32 519 Fragments arranged by size: 1171 630 519 76 45 32 32 32 32 32 29 27 13 5 5 5 KasI G'GCGC_C Cuts at: 0 73 2484 2685 Size: 73 2411 201 Fragments arranged by size: 2411 201 73 KpnI G_GTAC'C Cuts at: 0 1995 2685 Size: 1995 690 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2364 2477 2685 Size: 2364 113 208 Fragments arranged by size: 2364 208 113 MscI TGG'CCA Cuts at: 0 2515 2685 Size: 2515 170 MslI CAynn'nnrTG Cuts at: 0 2000 2414 2431 2685 Size: 2000 414 17 254 Fragments arranged by size: 2000 414 254 17 MspA1I CmG'CkG Cuts at: 0 42 553 834 1011 1093 1401 2685 Size: 42 511 281 177 82 308 1284 Fragments arranged by size: 1284 511 308 281 177 82 42 NarI GG'CG_CC Cuts at: 0 74 2485 2685 Size: 74 2411 200 Fragments arranged by size: 2411 200 74 NcoI C'CATG_G Cuts at: 0 713 1758 1891 2685 Size: 713 1045 133 794 Fragments arranged by size: 1045 794 713 133 NruI TCG'CGA Cuts at: 0 140 2685 Size: 140 2545 NspI r_CATG'y Cuts at: 0 2424 2542 2685 Size: 2424 118 143 Fragments arranged by size: 2424 143 118 PasI CC'CwG_GG Cuts at: 0 909 1951 2685 Size: 909 1042 734 Fragments arranged by size: 1042 909 734 PciI A'CATG_T Cuts at: 0 2420 2685 Size: 2420 265 PflMI CCAn_nnn'nTGG Cuts at: 0 343 816 2685 Size: 343 473 1869 Fragments arranged by size: 1869 473 343 PfoI T'CCnGG_A Cuts at: 0 1598 2241 2685 Size: 1598 643 444 Fragments arranged by size: 1598 643 444 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 512 544 710 742 1652 1684 2685 Size: 512 32 166 32 910 32 1001 Fragments arranged by size: 1001 910 512 166 32 32 32 PpuMI rG'GwC_Cy Cuts at: 0 658 1449 1764 1881 2685 Size: 658 791 315 117 804 Fragments arranged by size: 804 791 658 315 117 PspOMI G'GGCC_C Cuts at: 0 382 681 690 795 2445 2685 Size: 382 299 9 105 1650 240 Fragments arranged by size: 1650 382 299 240 105 9 PspXI vC'TCGA_Gb Cuts at: 0 662 2685 Size: 662 2023 PstI C_TGCA'G Cuts at: 0 164 308 833 1251 1535 1622 2223 2685 Size: 164 144 525 418 284 87 601 462 Fragments arranged by size: 601 525 462 418 284 164 144 87 PvuII CAG'CTG Cuts at: 0 42 553 834 1093 1401 2685 Size: 42 511 281 259 308 1284 Fragments arranged by size: 1284 511 308 281 259 42 SacI G_AGCT'C Cuts at: 0 123 530 1039 2685 Size: 123 407 509 1646 Fragments arranged by size: 1646 509 407 123 SapI GCTCTTCn'nnn_ Cuts at: 0 2353 2685 Size: 2353 332 SbfI CC_TGCA'GG Cuts at: 0 1535 1622 2685 Size: 1535 87 1063 Fragments arranged by size: 1535 1063 87 SfcI C'TryA_G Cuts at: 0 160 304 701 829 1247 1531 1618 2219 Size: 160 144 397 128 418 284 87 601 Cuts at: 2219 2685 Size: 466 Fragments arranged by size: 601 466 418 397 284 160 144 128 87 SfiI GGCCn_nnn'nGGCC Cuts at: 0 689 2685 Size: 689 1996 SfoI GGC'GCC Cuts at: 0 75 2486 2685 Size: 75 2411 199 Fragments arranged by size: 2411 199 75 SmlI C'TyrA_G Cuts at: 0 662 1393 1471 2558 2685 Size: 662 731 78 1087 127 Fragments arranged by size: 1087 731 662 127 78 StuI AGG'CCT Cuts at: 0 341 2685 Size: 341 2344 StyI C'CwwG_G Cuts at: 0 342 348 713 1758 1847 1891 2119 2685 Size: 342 6 365 1045 89 44 228 566 Fragments arranged by size: 1045 566 365 342 228 89 44 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 383 394 2685 Size: 383 11 2291 Fragments arranged by size: 2291 383 11 TatI w'GTAC_w Cuts at: 0 1769 2685 Size: 1769 916 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2647 2685 Size: 2647 38 XhoI C'TCGA_G Cuts at: 0 662 2685 Size: 662 2023 XmnI GAAnn'nnTTC Cuts at: 0 1375 1786 2685 Size: 1375 411 899 Fragments arranged by size: 1375 899 411 Enzymes that do cut: Acc65I AcuI AflIII AhdI AlfI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NruI NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SapI SbfI SfcI SfiI SfoI SmlI StuI StyI TaqII TatI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AleI ApaLI AscI AseI AsiSI AvrII BaeI BcgI BclI BfrBI BmtI BsaAI BsaBI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI DraI EcoRV FseI HincII HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI NsiI PacI PmeI PmlI PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XmaI ZraI