Restriction map for pFN21AB4690
 (Linear) (Six-base) MAPSORT of: KIBB4690.seq  Check: 6133  from: 1  to: 2685



KIBB4690.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

Acc65I G'GTAC_C

Cuts at:      0    1991    2685 
   Size:       1991     694
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     460     535     856    1139    1822    2510    2562 
   Size:         79     381      75     321     283     683     688      52

Cuts at:   2562    2685 
   Size:        123

  Fragments arranged by size: 

                 688     683     381     321     283     123      79      75
                  52
 
AflIII A'CryG_T

Cuts at:      0    2054    2420    2685 
   Size:       2054     366     265

  Fragments arranged by size: 

                2054     366     265
 
AhdI GACnn_n'nnGTC

Cuts at:      0     945    2685 
   Size:        945    1740
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     602    1546    2685 
   Size:        602     944    1139

  Fragments arranged by size: 

                1139     944     602
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     710     742    2685 
   Size:        710      32    1943

  Fragments arranged by size: 

                1943     710      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     931    1093    1710    2263    2380    2685 
   Size:        931     162     617     553     117     305

  Fragments arranged by size: 

                 931     617     553     305     162     117
 
ApaI G_GGCC'C

Cuts at:      0     386     685     694     799    2449    2685 
   Size:        386     299       9     105    1650     236

  Fragments arranged by size: 

                1650     386     299     236     105       9
 
ApoI r'AATT_y

Cuts at:      0     266     375     861    1130    2077    2685 
   Size:        266     109     486     269     947     608

  Fragments arranged by size: 

                 947     608     486     269     266     109
 
AvaI C'yCGr_G

Cuts at:      0      16     662    2685 
   Size:         16     646    2023

  Fragments arranged by size: 

                2023     646      16
 
BamHI G'GATC_C

Cuts at:      0    2505    2685 
   Size:       2505     180
 
BanI G'GyrC_C

Cuts at:      0      73     598    1991    2484    2685 
   Size:         73     525    1393     493     201

  Fragments arranged by size: 

                1393     525     493     201      73
 
BanII G_rGCy'C

Cuts at:      0     123     386     530     685     694     723     799    1039 
   Size:        123     263     144     155       9      29      76     240

Cuts at:   1039    1142    2449    2685 
   Size:        103    1307     236

  Fragments arranged by size: 

                1307     263     240     236     155     144     123     103
                  76      29       9
 
BbeI G_GCGC'C

Cuts at:      0      77    2488    2685 
   Size:         77    2411     197

  Fragments arranged by size: 

                2411     197      77
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1645    2192    2685 
   Size:       1645     547     493

  Fragments arranged by size: 

                1645     547     493
 
BbvCI CC'TCA_GC

Cuts at:      0    2296    2342    2685 
   Size:       2296      46     343

  Fragments arranged by size: 

                2296     343      46
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1943    2685 
   Size:       1943     742
 
BglI GCCn_nnn'nGGC

Cuts at:      0      71     106     182     689    2444    2654    2685 
   Size:         71      35      76     507    1755     210      31

  Fragments arranged by size: 

                1755     507     210      76      71      35      31
 
BglII A'GATC_T

Cuts at:      0    1821    2685 
   Size:       1821     864
 
BlpI GC'TnA_GC

Cuts at:      0     449    2594    2633    2685 
   Size:        449    2145      39      52

  Fragments arranged by size: 

                2145     449      52      39
 
Bme1580I G_kGCm'C

Cuts at:      0     386     601     685     694     799    2449    2685 
   Size:        386     215      84       9     105    1650     236

  Fragments arranged by size: 

                1650     386     236     215     105      84       9
 
BmgBI CAC'GTC

Cuts at:      0    2055    2685 
   Size:       2055     630
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     598     679    2042    2685 
   Size:        598      81    1363     643

  Fragments arranged by size: 

                1363     643     598      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1074    2685 
   Size:       1074    1611
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     583     659     779    1258    2003    2264    2685 
   Size:         44     539      76     120     479     745     261     421

  Fragments arranged by size: 

                 745     539     479     421     261     120      76      44
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1454    1837    2296    2342    2456    2685 
   Size:       1454     383     459      46     114     229

  Fragments arranged by size: 

                1454     459     383     229     114      46
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1378    1456    2543    2685 
   Size:       1378      78    1087     142

  Fragments arranged by size: 

                1378    1087     142      78
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2269    2685 
   Size:       2269     416
 
BsaHI Gr'CG_yC

Cuts at:      0      74    2485    2685 
   Size:         74    2411     200

  Fragments arranged by size: 

                2411     200      74
 
BsaWI w'CCGG_w

Cuts at:      0     971    2685 
   Size:        971    1714
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     710     740    2665    2685 
   Size:        710      30    1925      20

  Fragments arranged by size: 

                1925     710      30      20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     157     302     383     442     445     496     641     949 
   Size:        157     145      81      59       3      51     145     308

Cuts at:    949    1291    2329    2548    2685 
   Size:        342    1038     219     137

  Fragments arranged by size: 

                1038     342     308     219     157     145     145     137
                  81      59      51       3
 
BseYI C'CCAG_C

Cuts at:      0    1007    1086    2006    2685 
   Size:       1007      79     920     679

  Fragments arranged by size: 

                1007     920     679      79
 
BsiEI CG_ry'CG

Cuts at:      0     202    2685 
   Size:        202    2483
 
BsiHKAI G_wGCw'C

Cuts at:      0     123     530    1039    1283    2600    2685 
   Size:        123     407     509     244    1317      85

  Fragments arranged by size: 

                1317     509     407     244     123      85
 
Bsp1286I G_dGCh'C

Cuts at:      0     123     386     530     601     685     694     723     799 
   Size:        123     263     144      71      84       9      29      76

Cuts at:    799    1039    1142    1283    2449    2600    2685 
   Size:        240     103     141    1166     151      85

  Fragments arranged by size: 

                1166     263     240     151     144     141     123     103
                  85      84      76      71      29       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1538    2685 
   Size:       1538    1147
 
BsrBI CCG'CTC

Cuts at:      0     156     235     435    2043    2685 
   Size:        156      79     200    1608     642

  Fragments arranged by size: 

                1608     642     200     156      79
 
BsrDI GCAATG_nn'

Cuts at:      0     463    2685 
   Size:        463    2222
 
BsrFI r'CCGG_y

Cuts at:      0     201    2685 
   Size:        201    2484
 
BssSI C'ACGA_G

Cuts at:      0     737    2685 
   Size:        737    1948
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1508    2002    2685 
   Size:       1508     494     683

  Fragments arranged by size: 

                1508     683     494
 
BstYI r'GATC_y

Cuts at:      0     803     824    1163    1821    2505    2685 
   Size:        803      21     339     658     684     180

  Fragments arranged by size: 

                 803     684     658     339     180      21
 
BtgI C'CryG_G

Cuts at:      0     713    1071    1758    1891    2426    2649    2685 
   Size:        713     358     687     133     535     223      36

  Fragments arranged by size: 

                 713     687     535     358     223     133      36
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2335    2685 
   Size:       2335     350
 
BtsI GCAGTG_nn'

Cuts at:      0    1311    2685 
   Size:       1311    1374
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     269     304     359     394    2685 
   Size:        269      35      55      35    2291

  Fragments arranged by size: 

                2291     269      55      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1437    2685 
   Size:       1437    1248
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1647    2685 
   Size:       1647    1038
 
EaeI y'GGCC_r

Cuts at:      0     199    2513    2685 
   Size:        199    2314     172

  Fragments arranged by size: 

                2314     199     172
 
EagI C'GGCC_G

Cuts at:      0     199    2685 
   Size:        199    2486
 
EarI CTCTTCn'nnn_

Cuts at:      0     848     872    1268    2096    2156    2162    2353    2362 
   Size:        848      24     396     828      60       6     191       9

Cuts at:   2362    2486    2685 
   Size:        124     199

  Fragments arranged by size: 

                 848     828     396     199     191     124      60      24
                   9       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     305    2685 
   Size:        305    2380
 
EcoICRI GAG'CTC

Cuts at:      0     121     528    1037    2685 
   Size:        121     407     509    1648

  Fragments arranged by size: 

                1648     509     407     121
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44      79     460     535     583     659     779     856 
   Size:         44      35     381      75      48      76     120      77

Cuts at:    856    1139    1258    1822    2003    2264    2510    2562    2685 
   Size:        283     119     564     181     261     246      52     123

  Fragments arranged by size: 

                 564     381     283     261     246     181     123     120
                 119      77      76      75      52      48      44      35
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     335    2685 
   Size:        335    2350
 
EcoO109I rG'GnC_Cy

Cuts at:      0     382     383     658     691     796    1449    1614    1764 
   Size:        382       1     275      33     105     653     165     150

Cuts at:   1764    1881    2445    2685 
   Size:        117     564     240

  Fragments arranged by size: 

                 653     564     382     275     240     165     150     117
                 105      33       1
 
EcoRI G'AATT_C

Cuts at:      0     266     861    2685 
   Size:        266     595    1824

  Fragments arranged by size: 

                1824     595     266
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1182    1578    2592    2685 
   Size:       1182     396    1014      93

  Fragments arranged by size: 

                1182    1014     396      93
 
FspI TGC'GCA

Cuts at:      0     646    2685 
   Size:        646    2039
 
FspAI rTGC'GCAy

Cuts at:      0     646    2685 
   Size:        646    2039
 
HaeII r_GCGC'y

Cuts at:      0      77    2482    2488    2685 
   Size:         77    2405       6     197

  Fragments arranged by size: 

                2405     197      77       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29     105     110     137     142     772     804     849 
   Size:         29      76       5      27       5     630      32      45

Cuts at:    849     881     886     918     931     963    2134    2166    2685 
   Size:         32       5      32      13      32    1171      32     519

  Fragments arranged by size: 

                1171     630     519      76      45      32      32      32
                  32      32      29      27      13       5       5       5
            
 
KasI G'GCGC_C

Cuts at:      0      73    2484    2685 
   Size:         73    2411     201

  Fragments arranged by size: 

                2411     201      73
 
KpnI G_GTAC'C

Cuts at:      0    1995    2685 
   Size:       1995     690
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2364    2477    2685 
   Size:       2364     113     208

  Fragments arranged by size: 

                2364     208     113
 
MscI TGG'CCA

Cuts at:      0    2515    2685 
   Size:       2515     170
 
MslI CAynn'nnrTG

Cuts at:      0    2000    2414    2431    2685 
   Size:       2000     414      17     254

  Fragments arranged by size: 

                2000     414     254      17
 
MspA1I CmG'CkG

Cuts at:      0      42     553     834    1011    1093    1401    2685 
   Size:         42     511     281     177      82     308    1284

  Fragments arranged by size: 

                1284     511     308     281     177      82      42
 
NarI GG'CG_CC

Cuts at:      0      74    2485    2685 
   Size:         74    2411     200

  Fragments arranged by size: 

                2411     200      74
 
NcoI C'CATG_G

Cuts at:      0     713    1758    1891    2685 
   Size:        713    1045     133     794

  Fragments arranged by size: 

                1045     794     713     133
 
NruI TCG'CGA

Cuts at:      0     140    2685 
   Size:        140    2545
 
NspI r_CATG'y

Cuts at:      0    2424    2542    2685 
   Size:       2424     118     143

  Fragments arranged by size: 

                2424     143     118
 
PasI CC'CwG_GG

Cuts at:      0     909    1951    2685 
   Size:        909    1042     734

  Fragments arranged by size: 

                1042     909     734
 
PciI A'CATG_T

Cuts at:      0    2420    2685 
   Size:       2420     265
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     343     816    2685 
   Size:        343     473    1869

  Fragments arranged by size: 

                1869     473     343
 
PfoI T'CCnGG_A

Cuts at:      0    1598    2241    2685 
   Size:       1598     643     444

  Fragments arranged by size: 

                1598     643     444
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     512     544     710     742    1652    1684    2685 
   Size:        512      32     166      32     910      32    1001

  Fragments arranged by size: 

                1001     910     512     166      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     658    1449    1764    1881    2685 
   Size:        658     791     315     117     804

  Fragments arranged by size: 

                 804     791     658     315     117
 
PspOMI G'GGCC_C

Cuts at:      0     382     681     690     795    2445    2685 
   Size:        382     299       9     105    1650     240

  Fragments arranged by size: 

                1650     382     299     240     105       9
 
PspXI vC'TCGA_Gb

Cuts at:      0     662    2685 
   Size:        662    2023
 
PstI C_TGCA'G

Cuts at:      0     164     308     833    1251    1535    1622    2223    2685 
   Size:        164     144     525     418     284      87     601     462

  Fragments arranged by size: 

                 601     525     462     418     284     164     144      87
            
 
PvuII CAG'CTG

Cuts at:      0      42     553     834    1093    1401    2685 
   Size:         42     511     281     259     308    1284

  Fragments arranged by size: 

                1284     511     308     281     259      42
 
SacI G_AGCT'C

Cuts at:      0     123     530    1039    2685 
   Size:        123     407     509    1646

  Fragments arranged by size: 

                1646     509     407     123
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2353    2685 
   Size:       2353     332
 
SbfI CC_TGCA'GG

Cuts at:      0    1535    1622    2685 
   Size:       1535      87    1063

  Fragments arranged by size: 

                1535    1063      87
 
SfcI C'TryA_G

Cuts at:      0     160     304     701     829    1247    1531    1618    2219 
   Size:        160     144     397     128     418     284      87     601

Cuts at:   2219    2685 
   Size:        466

  Fragments arranged by size: 

                 601     466     418     397     284     160     144     128
                  87
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     689    2685 
   Size:        689    1996
 
SfoI GGC'GCC

Cuts at:      0      75    2486    2685 
   Size:         75    2411     199

  Fragments arranged by size: 

                2411     199      75
 
SmlI C'TyrA_G

Cuts at:      0     662    1393    1471    2558    2685 
   Size:        662     731      78    1087     127

  Fragments arranged by size: 

                1087     731     662     127      78
 
StuI AGG'CCT

Cuts at:      0     341    2685 
   Size:        341    2344
 
StyI C'CwwG_G

Cuts at:      0     342     348     713    1758    1847    1891    2119    2685 
   Size:        342       6     365    1045      89      44     228     566

  Fragments arranged by size: 

                1045     566     365     342     228      89      44       6
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     383     394    2685 
   Size:        383      11    2291

  Fragments arranged by size: 

                2291     383      11
 
TatI w'GTAC_w

Cuts at:      0    1769    2685 
   Size:       1769     916
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2647    2685 
   Size:       2647      38
 
XhoI C'TCGA_G

Cuts at:      0     662    2685 
   Size:        662    2023
 
XmnI GAAnn'nnTTC

Cuts at:      0    1375    1786    2685 
   Size:       1375     411     899

  Fragments arranged by size: 

                1375     899     411
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AhdI     AlfI     AloI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BssSI    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    FspAI    HaeII    Hin4I     KasI
     KpnI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NruI
     NspI     PasI     PciI    PflMI     PfoI     PpiI    PpuMI   PspOMI
    PspXI     PstI    PvuII     SacI     SapI     SbfI     SfcI     SfiI
     SfoI     SmlI     StuI     StyI    TaqII     TatI     XcmI     XhoI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AleI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI     BclI
    BfrBI     BmtI    BsaAI    BsaBI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     ClaI     DraI    EcoRV     FseI   HincII  HindIII     HpaI
     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NsiI
     PacI     PmeI     PmlI    PshAI     PsiI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     ScaI    SexAI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XmaI     ZraI
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