(Circular) (Six-base) MAPSORT of: pFN21AB4677.seq Check: 9859 from: 1 to: 7618 pFN21AB4677 7618 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2086 5428 278 Size: 53 83 186 936 550 3342 2468 Fragments arranged by size: 3342 2468 936 550 186 83 53 AccI GT'mk_AC Cuts at: 1937 4441 4586 4811 4961 1937 Size: 2504 145 225 150 4594 Fragments arranged by size: 4594 2504 225 150 145 Acc65I G'GTAC_C Cuts at: 4887 4939 4887 Size: 52 7566 AclI AA'CG_TT Cuts at: 1563 5745 6118 1563 Size: 4182 373 3063 Fragments arranged by size: 4182 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2175 2955 3687 4058 4322 4877 5681 7065 Size: 543 780 732 371 264 555 804 1384 Cuts at: 7065 7333 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 804 780 732 555 543 371 268 264 AfeI AGC'GCT Cuts at: 4869 4869 Size: 7618 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7421 AflIII A'CryG_T Cuts at: 1203 3855 6517 7494 1203 Size: 2652 2662 977 1327 Fragments arranged by size: 2662 2652 1327 977 AgeI A'CCGG_T Cuts at: 6449 6449 Size: 7618 AhdI GACnn_n'nnGTC Cuts at: 4039 6347 4039 Size: 2308 5310 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7618 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7618 AlwNI CAG_nnn'CTG Cuts at: 4213 5361 6933 4213 Size: 1148 1572 4898 Fragments arranged by size: 4898 1572 1148 ApaLI G'TGCA_C Cuts at: 2294 5675 6831 2294 Size: 3381 1156 3081 Fragments arranged by size: 3381 3081 1156 ApoI r'AATT_y Cuts at: 1493 2096 2253 2322 2492 2690 2858 2899 3062 Size: 603 157 69 170 198 168 41 163 Cuts at: 3062 3431 3919 3977 4182 4342 4491 4779 4927 Size: 369 488 58 205 160 149 288 148 Cuts at: 4927 5153 5305 7414 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 603 488 369 288 226 205 198 170 168 163 160 157 152 149 148 69 58 41 AseI AT'TA_AT Cuts at: 160 1032 2442 3528 4386 6172 160 Size: 872 1410 1086 858 1786 1606 Fragments arranged by size: 1786 1606 1410 1086 872 858 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7618 AvaI C'yCGr_G Cuts at: 1870 1943 4943 1870 Size: 73 3000 4545 Fragments arranged by size: 4545 3000 73 BamHI G'GATC_C Cuts at: 4948 5315 4948 Size: 367 7251 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4887 4939 6394 618 Size: 325 217 627 3100 52 1455 1842 Fragments arranged by size: 3100 1842 1455 627 325 217 52 BanII G_rGCy'C Cuts at: 1391 2802 4937 1391 Size: 1411 2135 4072 Fragments arranged by size: 4072 2135 1411 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4035 928 Size: 971 2136 4511 Fragments arranged by size: 4511 2136 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3165 3199 5809 5843 1903 Size: 34 1228 34 2610 34 3678 Fragments arranged by size: 3678 2610 1228 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5510 6720 5510 Size: 1210 6408 BclI T'GATC_A Cuts at: 1548 1548 Size: 7618 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6229 136 Size: 107 122 71 631 605 4557 1525 Fragments arranged by size: 4557 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4524 7613 4524 Size: 3089 4529 BlpI GC'TnA_GC Cuts at: 2306 5035 2306 Size: 2729 4889 Bme1580I G_kGCm'C Cuts at: 1790 2298 4414 5679 6835 1790 Size: 508 2116 1265 1156 2573 Fragments arranged by size: 2573 2116 1265 1156 508 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7618 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5349 6307 442 Size: 416 53 83 399 24 3932 958 1753 Fragments arranged by size: 3932 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7618 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2245 4856 6278 1239 Size: 935 71 2611 1422 2579 Fragments arranged by size: 2611 2579 1422 935 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5743 6608 6906 5743 Size: 865 298 6455 Fragments arranged by size: 6455 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2977 6281 882 Size: 617 1478 3304 2219 Fragments arranged by size: 3304 2219 1478 617 BsaAI yAC'GTr Cuts at: 493 1204 5409 493 Size: 711 4205 2702 Fragments arranged by size: 4205 2702 711 BsaBI GATnn'nnATC Cuts at: 2022 2067 7612 2022 Size: 45 5545 2028 Fragments arranged by size: 5545 2028 45 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2083 5425 5807 6442 Size: 53 83 186 936 550 3342 382 635 Cuts at: 6442 275 Size: 1451 Fragments arranged by size: 3342 1451 936 635 550 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6051 6449 6723 6870 7281 6051 Size: 398 274 147 411 6388 Fragments arranged by size: 6388 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3920 3950 1178 Size: 30 165 30 2517 30 4846 Fragments arranged by size: 4846 2517 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3119 3941 1186 Size: 1933 822 4863 Fragments arranged by size: 4863 1933 822 BseYI C'CCAG_C Cuts at: 4730 6821 7317 4730 Size: 2091 496 5031 Fragments arranged by size: 5031 2091 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7045 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2269 5068 5829 6857 1540 Size: 275 186 268 2799 761 1028 2301 Fragments arranged by size: 2799 2301 1028 761 275 268 186 BsiHKAI G_wGCw'C Cuts at: 2298 2802 4275 4850 5679 5764 6835 2298 Size: 504 1473 575 829 85 1071 3081 Fragments arranged by size: 3081 1473 1071 829 575 504 85 BsmI GAATG_Cn' Cuts at: 4133 4864 5129 5222 4133 Size: 731 265 93 6529 Fragments arranged by size: 6529 731 265 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2298 2802 4275 4414 4850 4937 5679 Size: 399 508 504 1473 139 436 87 742 Cuts at: 5679 5764 6835 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1473 1071 742 508 504 436 399 139 87 85 BspEI T'CCGG_A Cuts at: 7281 7281 Size: 7618 BspHI T'CATG_A Cuts at: 2488 3732 5507 7237 2488 Size: 1244 1775 1730 2869 Fragments arranged by size: 2869 1775 1730 1244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2880 4973 844 Size: 2036 2093 3489 Fragments arranged by size: 3489 2093 2036 BsrBI CCG'CTC Cuts at: 1394 1823 5505 1394 Size: 429 3682 3507 Fragments arranged by size: 3682 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6113 6287 65 Size: 1183 4865 174 1396 Fragments arranged by size: 4865 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2786 6262 6449 1700 Size: 110 976 3476 187 2869 Fragments arranged by size: 3476 2869 976 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7618 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7618 BssSI C'ACGA_G Cuts at: 5678 6690 5678 Size: 1012 6606 BstAPI GCAn_nnn'nTGC Cuts at: 794 2302 2606 2837 3575 4169 794 Size: 1508 304 231 738 594 4243 Fragments arranged by size: 4243 1508 738 594 304 231 BstBI TT'CG_AA Cuts at: 6422 6422 Size: 7618 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7618 BstXI CCAn_nnnn'nTGG Cuts at: 2382 4086 2382 Size: 1704 5914 BstYI r'GATC_y Cuts at: 1972 2680 3131 4524 4948 5315 5700 5717 7169 Size: 708 451 1393 424 367 385 17 1452 Cuts at: 7169 7255 7267 7613 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1393 708 451 424 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 4442 4587 4442 Size: 145 7473 Bsu36I CC'TnA_GG Cuts at: 2618 2618 Size: 7618 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6127 Fragments arranged by size: 6127 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2031 487 Size: 683 56 805 6074 Fragments arranged by size: 6074 805 683 56 BtsI GCAGTG_nn' Cuts at: 4910 5134 5928 5948 4910 Size: 224 794 20 6580 Fragments arranged by size: 6580 794 224 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7618 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7583 DraI TTT'AAA Cuts at: 2049 2476 3411 4922 2049 Size: 427 935 1511 4745 Fragments arranged by size: 4745 1511 935 427 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6625 809 Size: 507 5309 1802 Fragments arranged by size: 5309 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2274 5065 5954 Size: 54 1425 50 275 18 444 2791 889 Cuts at: 5954 7583 8 Size: 1629 43 Fragments arranged by size: 2791 1629 1425 889 444 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5065 1537 Size: 275 3253 4090 Fragments arranged by size: 4090 3253 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2772 3706 5548 1353 Size: 1419 934 1842 3423 Fragments arranged by size: 3423 1842 1419 934 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6205 6579 6725 387 Size: 1064 138 4616 374 146 1280 Fragments arranged by size: 4616 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2800 2800 Size: 7618 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2175 2245 2955 3687 4058 4322 Size: 393 542 1 70 710 732 371 264 Cuts at: 4322 4856 4877 5681 6278 7065 7333 1239 Size: 534 21 804 597 787 268 1524 Fragments arranged by size: 1524 804 787 732 710 597 542 534 393 371 268 264 70 21 1 EcoNI CCTnn'n_nnAGG Cuts at: 3207 3207 Size: 7618 EcoO109I rG'GnC_Cy Cuts at: 3200 3605 3200 Size: 405 7213 EcoRI G'AATT_C Cuts at: 2858 4342 4927 5305 2858 Size: 1484 585 378 5171 Fragments arranged by size: 5171 1484 585 378 EcoRV GAT'ATC Cuts at: 4700 4700 Size: 7618 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3518 3906 4907 3518 Size: 388 1001 6229 Fragments arranged by size: 6229 1001 388 FspI TGC'GCA Cuts at: 6124 6124 Size: 7618 HaeII r_GCGC'y Cuts at: 1678 4871 6765 1678 Size: 3193 1894 2531 Fragments arranged by size: 3193 2531 1894 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3398 3430 3517 3549 4025 Size: 32 135 32 1826 32 87 32 476 Cuts at: 4025 4057 6259 6291 6333 6365 1373 Size: 32 2202 32 42 32 2626 Fragments arranged by size: 2626 2202 1826 476 135 87 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3271 4294 4962 5208 669 Size: 988 281 1333 1023 668 246 3079 Fragments arranged by size: 3079 1333 1023 988 668 281 246 HindIII A'AGCT_T Cuts at: 748 2525 2756 748 Size: 1777 231 5610 Fragments arranged by size: 5610 1777 231 HpaI GTT'AAC Cuts at: 5208 5208 Size: 7618 KpnI G_GTAC'C Cuts at: 4891 4943 4891 Size: 52 7566 MfeI C'AATT_G Cuts at: 5217 5217 Size: 7618 MluI A'CGCG_T Cuts at: 7494 7494 Size: 7618 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2104 6732 6916 1305 Size: 799 4628 184 2007 Fragments arranged by size: 4628 2007 799 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2276 10 Size: 54 1425 343 444 5352 Fragments arranged by size: 5352 1425 444 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2380 2601 3285 4029 5576 5935 6094 Size: 648 1214 221 684 744 1547 359 159 Cuts at: 6094 518 Size: 2042 Fragments arranged by size: 2042 1547 1214 744 684 648 359 221 159 MspA1I CmG'CkG Cuts at: 1606 2090 3632 4175 5034 5711 6859 7104 1606 Size: 484 1542 543 859 677 1148 245 2120 Fragments arranged by size: 2120 1542 1148 859 677 543 484 245 NaeI GCC'GGC Cuts at: 2788 2788 Size: 7618 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6127 Fragments arranged by size: 6127 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7618 NgoMIV G'CCGG_C Cuts at: 2786 2786 Size: 7618 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7618 NotI GC'GGCC_GC Cuts at: 5065 5065 Size: 7618 NspI r_CATG'y Cuts at: 1132 1229 3859 4279 4976 6521 1132 Size: 97 2630 420 697 1545 2229 Fragments arranged by size: 2630 2229 1545 697 420 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7618 PciI A'CATG_T Cuts at: 3855 6517 3855 Size: 2662 4956 PflMI CCAn_nnn'nTGG Cuts at: 1108 4596 1108 Size: 3488 4130 PfoI T'CCnGG_A Cuts at: 4822 4822 Size: 7618 PmeI GTTT'AAAC Cuts at: 4922 4922 Size: 7618 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5687 5719 7200 7232 5687 Size: 32 1481 32 6073 Fragments arranged by size: 6073 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3200 3200 Size: 7618 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7618 PsiI TTA'TAA Cuts at: 2994 5188 2994 Size: 2194 5424 PstI C_TGCA'G Cuts at: 830 2591 3246 3637 4174 4970 830 Size: 1761 655 391 537 796 3478 Fragments arranged by size: 3478 1761 796 655 537 391 PvuI CG_AT'CG Cuts at: 2001 2269 2001 Size: 268 7350 PvuII CAG'CTG Cuts at: 2090 3632 4175 2090 Size: 1542 543 5533 Fragments arranged by size: 5533 1542 543 SacI G_AGCT'C Cuts at: 2802 2802 Size: 7618 SalI G'TCGA_C Cuts at: 1936 4960 1936 Size: 3024 4594 SapI GCTCTTCn'nnn_ Cuts at: 3706 3706 Size: 7618 SbfI CC_TGCA'GG Cuts at: 4970 4970 Size: 7618 ScaI AGT'ACT Cuts at: 5866 7578 5866 Size: 1712 5906 SfcI C'TryA_G Cuts at: 826 1045 2587 2662 3242 3633 4170 4752 4966 Size: 219 1542 75 580 391 537 582 214 Cuts at: 4966 6101 6782 6973 826 Size: 1135 681 191 1471 Fragments arranged by size: 1542 1471 1135 681 582 580 537 391 219 214 191 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7618 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7618 SmaI CCC'GGG Cuts at: 1872 4945 1872 Size: 3073 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5722 6623 6885 820 Size: 197 926 3779 901 262 1553 Fragments arranged by size: 3779 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5409 493 Size: 4916 2702 SpeI A'CTAG_T Cuts at: 152 2905 152 Size: 2753 4865 SphI G_CATG'C Cuts at: 1132 4976 1132 Size: 3844 3774 SspI AAT'ATT Cuts at: 5 52 2045 4105 4431 5542 5 Size: 47 1993 2060 326 1111 2081 Fragments arranged by size: 2081 2060 1993 1111 326 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7618 StyI C'CwwG_G Cuts at: 513 1065 2004 4240 4882 5057 513 Size: 552 939 2236 642 175 3074 Fragments arranged by size: 3074 2236 939 642 552 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5645 5662 5815 6000 6445 325 Size: 1255 4065 17 153 185 445 1498 Fragments arranged by size: 4065 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3108 5864 7576 Size: 274 80 33 51 1443 1131 2756 1712 Cuts at: 7576 96 Size: 138 Fragments arranged by size: 2756 1712 1443 1131 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7578 7610 7578 Size: 32 7586 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7618 XbaI T'CTAG_A Cuts at: 4954 4954 Size: 7618 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2441 3270 4783 2441 Size: 829 1513 5276 Fragments arranged by size: 5276 1513 829 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7618 XmaI C'CCGG_G Cuts at: 1870 4943 1870 Size: 3073 4545 XmnI GAAnn'nnTTC Cuts at: 2370 3742 3981 5747 2370 Size: 1372 239 1766 4241 Fragments arranged by size: 4241 1766 1372 239 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2084 5426 276 Size: 53 83 186 936 550 3342 2468 Fragments arranged by size: 3342 2468 936 550 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI