Restriction map for
pFN21AB4677
(Linear) (Six-base) MAPSORT of: KIBB4677.seq Check: 6264 from: 1 to: 2913
KIBB4677.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AatII G_ACGT'C
Cuts at: 0 81 2913
Size: 81 2832
AccI GT'mk_AC
Cuts at: 0 2436 2581 2806 2913
Size: 2436 145 225 107
Fragments arranged by size:
2436 225 145 107
Acc65I G'GTAC_C
Cuts at: 0 2882 2913
Size: 2882 31
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 950 1682 2053 2317 2872 2913
Size: 170 780 732 371 264 555 41
Fragments arranged by size:
780 732 555 371 264 170 41
AfeI AGC'GCT
Cuts at: 0 2864 2913
Size: 2864 49
AflIII A'CryG_T
Cuts at: 0 1850 2913
Size: 1850 1063
AhdI GACnn_n'nnGTC
Cuts at: 0 2034 2913
Size: 2034 879
AlwNI CAG_nnn'CTG
Cuts at: 0 2208 2913
Size: 2208 705
ApaLI G'TGCA_C
Cuts at: 0 289 2913
Size: 289 2624
ApoI r'AATT_y
Cuts at: 0 91 248 317 487 685 853 894 1057
Size: 91 157 69 170 198 168 41 163
Cuts at: 1057 1426 1914 1972 2177 2337 2486 2774 2913
Size: 369 488 58 205 160 149 288 139
Fragments arranged by size:
488 369 288 205 198 170 168 163
160 157 149 139 91 69 58 41
AseI AT'TA_AT
Cuts at: 0 437 1523 2381 2913
Size: 437 1086 858 532
Fragments arranged by size:
1086 858 532 437
BanI G'GyrC_C
Cuts at: 0 2882 2913
Size: 2882 31
BanII G_rGCy'C
Cuts at: 0 797 2913
Size: 797 2116
BbsI GAAGACnn'nnnn_
Cuts at: 0 2030 2913
Size: 2030 883
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1160 1194 2913
Size: 1160 34 1719
Fragments arranged by size:
1719 1160 34
BglII A'GATC_T
Cuts at: 0 2519 2913
Size: 2519 394
BlpI GC'TnA_GC
Cuts at: 0 301 2913
Size: 301 2612
Bme1580I G_kGCm'C
Cuts at: 0 293 2409 2913
Size: 293 2116 504
Fragments arranged by size:
2116 504 293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 240 2851 2913
Size: 169 71 2611 62
Fragments arranged by size:
2611 169 71 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 972 2913
Size: 972 1941
BsaBI GATnn'nnATC
Cuts at: 0 17 62 2913
Size: 17 45 2851
Fragments arranged by size:
2851 45 17
BsaHI Gr'CG_yC
Cuts at: 0 78 2913
Size: 78 2835
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1915 1945 2913
Size: 1915 30 968
Fragments arranged by size:
1915 968 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1114 1936 2913
Size: 1114 822 977
Fragments arranged by size:
1114 977 822
BseYI C'CCAG_C
Cuts at: 0 2725 2913
Size: 2725 188
BsiEI CG_ry'CG
Cuts at: 0 264 2913
Size: 264 2649
BsiHKAI G_wGCw'C
Cuts at: 0 293 797 2270 2845 2913
Size: 293 504 1473 575 68
Fragments arranged by size:
1473 575 504 293 68
BsmI GAATG_Cn'
Cuts at: 0 2128 2859 2913
Size: 2128 731 54
Fragments arranged by size:
2128 731 54
Bsp1286I G_dGCh'C
Cuts at: 0 293 797 2270 2409 2845 2913
Size: 293 504 1473 139 436 68
Fragments arranged by size:
1473 504 436 293 139 68
BspHI T'CATG_A
Cuts at: 0 483 1727 2913
Size: 483 1244 1186
Fragments arranged by size:
1244 1186 483
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 875 2913
Size: 875 2038
BsrFI r'CCGG_y
Cuts at: 0 781 2913
Size: 781 2132
BstAPI GCAn_nnn'nTGC
Cuts at: 0 297 601 832 1570 2164 2913
Size: 297 304 231 738 594 749
Fragments arranged by size:
749 738 594 304 297 231
BstXI CCAn_nnnn'nTGG
Cuts at: 0 377 2081 2913
Size: 377 1704 832
Fragments arranged by size:
1704 832 377
BstYI r'GATC_y
Cuts at: 0 675 1126 2519 2913
Size: 675 451 1393 394
Fragments arranged by size:
1393 675 451 394
BstZ17I GTA'TAC
Cuts at: 0 2437 2582 2913
Size: 2437 145 331
Fragments arranged by size:
2437 331 145
Bsu36I CC'TnA_GG
Cuts at: 0 613 2913
Size: 613 2300
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 26 2913
Size: 26 2887
BtsI GCAGTG_nn'
Cuts at: 0 2905 2913
Size: 2905 8
DraI TTT'AAA
Cuts at: 0 44 471 1406 2913
Size: 44 427 935 1507
Fragments arranged by size:
1507 935 427 44
EaeI y'GGCC_r
Cuts at: 0 269 2913
Size: 269 2644
EarI CTCTTCn'nnn_
Cuts at: 0 767 1701 2913
Size: 767 934 1212
Fragments arranged by size:
1212 934 767
EcoICRI GAG'CTC
Cuts at: 0 795 2913
Size: 795 2118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 170 240 950 1682 2053 2317 2851
Size: 169 1 70 710 732 371 264 534
Cuts at: 2851 2872 2913
Size: 21 41
Fragments arranged by size:
732 710 534 371 264 169 70 41
21 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1202 2913
Size: 1202 1711
EcoO109I rG'GnC_Cy
Cuts at: 0 1195 1600 2913
Size: 1195 405 1313
Fragments arranged by size:
1313 1195 405
EcoRI G'AATT_C
Cuts at: 0 853 2337 2913
Size: 853 1484 576
Fragments arranged by size:
1484 853 576
EcoRV GAT'ATC
Cuts at: 0 2695 2913
Size: 2695 218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1513 1901 2902 2913
Size: 1513 388 1001 11
Fragments arranged by size:
1513 1001 388 11
HaeII r_GCGC'y
Cuts at: 0 2866 2913
Size: 2866 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1393 1425 1512 1544 2020 2052 2913
Size: 1393 32 87 32 476 32 861
Fragments arranged by size:
1393 861 476 87 32 32 32
HincII GTy'rAC
Cuts at: 0 1266 2289 2913
Size: 1266 1023 624
Fragments arranged by size:
1266 1023 624
HindIII A'AGCT_T
Cuts at: 0 520 751 2913
Size: 520 231 2162
Fragments arranged by size:
2162 520 231
KpnI G_GTAC'C
Cuts at: 0 2886 2913
Size: 2886 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 2913
Size: 99 2814
MscI TGG'CCA
Cuts at: 0 271 2913
Size: 271 2642
MslI CAynn'nnrTG
Cuts at: 0 375 596 1280 2024 2913
Size: 375 221 684 744 889
Fragments arranged by size:
889 744 684 375 221
MspA1I CmG'CkG
Cuts at: 0 85 1627 2170 2913
Size: 85 1542 543 743
Fragments arranged by size:
1542 743 543 85
NaeI GCC'GGC
Cuts at: 0 783 2913
Size: 783 2130
NgoMIV G'CCGG_C
Cuts at: 0 781 2913
Size: 781 2132
NspI r_CATG'y
Cuts at: 0 1854 2274 2913
Size: 1854 420 639
Fragments arranged by size:
1854 639 420
PciI A'CATG_T
Cuts at: 0 1850 2913
Size: 1850 1063
PflMI CCAn_nnn'nTGG
Cuts at: 0 2591 2913
Size: 2591 322
PfoI T'CCnGG_A
Cuts at: 0 2817 2913
Size: 2817 96
PpuMI rG'GwC_Cy
Cuts at: 0 1195 2913
Size: 1195 1718
PsiI TTA'TAA
Cuts at: 0 989 2913
Size: 989 1924
PstI C_TGCA'G
Cuts at: 0 586 1241 1632 2169 2913
Size: 586 655 391 537 744
Fragments arranged by size:
744 655 586 537 391
PvuI CG_AT'CG
Cuts at: 0 264 2913
Size: 264 2649
PvuII CAG'CTG
Cuts at: 0 85 1627 2170 2913
Size: 85 1542 543 743
Fragments arranged by size:
1542 743 543 85
SacI G_AGCT'C
Cuts at: 0 797 2913
Size: 797 2116
SapI GCTCTTCn'nnn_
Cuts at: 0 1701 2913
Size: 1701 1212
SfcI C'TryA_G
Cuts at: 0 582 657 1237 1628 2165 2747 2913
Size: 582 75 580 391 537 582 166
Fragments arranged by size:
582 582 580 537 391 166 75
SpeI A'CTAG_T
Cuts at: 0 900 2913
Size: 900 2013
SspI AAT'ATT
Cuts at: 0 40 2100 2426 2913
Size: 40 2060 326 487
Fragments arranged by size:
2060 487 326 40
StyI C'CwwG_G
Cuts at: 0 2235 2877 2913
Size: 2235 642 36
Fragments arranged by size:
2235 642 36
TatI w'GTAC_w
Cuts at: 0 1103 2913
Size: 1103 1810
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 436 1265 2778 2913
Size: 436 829 1513 135
Fragments arranged by size:
1513 829 436 135
XmnI GAAnn'nnTTC
Cuts at: 0 365 1737 1976 2913
Size: 365 1372 239 937
Fragments arranged by size:
1372 937 365 239
ZraI GAC'GTC
Cuts at: 0 79 2913
Size: 79 2834
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflIII AhdI AlwNI
ApaLI ApoI AseI BanI BanII BbsI BcgI BglII
BlpI Bme1580I BpmI BsaI BsaBI BsaHI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI
BstAPI BstXI BstYI BstZ17I Bsu36I BtgZI BtsI DraI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII KpnI MmeI MscI
MslI MspA1I NaeI NgoMIV NspI PciI PflMI PfoI
PpuMI PsiI PstI PvuI PvuII SacI SapI SfcI
SpeI SspI StyI TatI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AgeI AleI AlfI AloI ApaI
AscI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BciVI BclI BfrBI BglI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaAI BsaWI BsgI BsiWI BsmBI BspEI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BtgI
ClaI CspCI DraIII DrdI EagI EciI FseI FspI
FspAI HpaI KasI MfeI MluI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SphI SrfI StuI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI