(Circular) (Six-base) MAPSORT of: pFN21AB4506.seq Check: 9089 from: 1 to: 6670 pFN21AB4506 6670 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4480 278 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4013 1937 Size: 2076 4594 Acc65I G'GTAC_C Cuts at: 2527 3991 2527 Size: 1464 5206 AclI AA'CG_TT Cuts at: 1563 4797 5170 1563 Size: 3234 373 3063 Fragments arranged by size: 3234 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3666 4733 6117 6385 1632 Size: 2034 1067 1384 268 1917 Fragments arranged by size: 2034 1917 1384 1067 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6473 AflIII A'CryG_T Cuts at: 1203 5569 6546 1203 Size: 4366 977 1327 Fragments arranged by size: 4366 1327 977 AgeI A'CCGG_T Cuts at: 5501 5501 Size: 6670 AhdI GACnn_n'nnGTC Cuts at: 5399 5399 Size: 6670 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6670 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3413 1184 Size: 2229 4441 AlwNI CAG_nnn'CTG Cuts at: 2405 2558 4413 5985 2405 Size: 153 1855 1572 3090 Fragments arranged by size: 3090 1855 1572 153 ApaI G_GGCC'C Cuts at: 3864 3864 Size: 6670 ApaLI G'TGCA_C Cuts at: 4727 5883 4727 Size: 1156 5514 ApoI r'AATT_y Cuts at: 1493 3979 4205 4357 6466 1493 Size: 2486 226 152 2109 1697 Fragments arranged by size: 2486 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5224 160 Size: 872 4192 1606 Fragments arranged by size: 4192 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 AvaI C'yCGr_G Cuts at: 1870 1943 3507 3516 3676 3995 1870 Size: 73 1564 9 160 319 4545 Fragments arranged by size: 4545 1564 319 160 73 9 AvrII C'CTAG_G Cuts at: 2491 2491 Size: 6670 BamHI G'GATC_C Cuts at: 2738 4000 4367 2738 Size: 1262 367 5041 Fragments arranged by size: 5041 1262 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2527 3991 5446 618 Size: 325 217 627 740 1464 1455 1842 Fragments arranged by size: 1842 1464 1455 740 627 325 217 BanII G_rGCy'C Cuts at: 1391 3864 3989 1391 Size: 2473 125 4072 Fragments arranged by size: 4072 2473 125 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3075 3261 3847 928 Size: 971 1176 186 586 3751 Fragments arranged by size: 3751 1176 971 586 186 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4861 4895 1903 Size: 34 2924 34 3678 Fragments arranged by size: 3678 2924 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4562 5772 4562 Size: 1210 5460 BclI T'GATC_A Cuts at: 1548 2614 1548 Size: 1066 5604 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2255 3708 5281 Size: 107 122 71 631 605 583 1453 1573 Cuts at: 5281 136 Size: 1525 Fragments arranged by size: 1573 1525 1453 631 605 583 122 107 71 BglII A'GATC_T Cuts at: 3034 6665 3034 Size: 3631 3039 BlpI GC'TnA_GC Cuts at: 4087 4087 Size: 6670 Bme1580I G_kGCm'C Cuts at: 1790 3864 4731 5887 1790 Size: 2074 867 1156 2573 Fragments arranged by size: 2573 2074 1156 867 BmgBI CAC'GTC Cuts at: 1318 3908 1318 Size: 2590 4080 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2814 3189 4401 Size: 416 53 83 399 24 1397 375 1212 Cuts at: 4401 5359 442 Size: 958 1753 Fragments arranged by size: 1753 1397 1212 958 416 399 375 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6670 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3749 Size: 6670 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2871 3003 3552 3579 3628 5330 1239 Size: 1632 132 549 27 49 1702 2579 Fragments arranged by size: 2579 1702 1632 549 132 49 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2067 4795 5660 5958 2067 Size: 2728 865 298 2779 Fragments arranged by size: 2779 2728 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5333 882 Size: 617 3834 2219 Fragments arranged by size: 3834 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4461 493 Size: 711 3257 2702 Fragments arranged by size: 3257 2702 711 BsaBI GATnn'nnATC Cuts at: 3039 3197 6664 3039 Size: 158 3467 3045 Fragments arranged by size: 3467 3045 158 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 4477 4859 5494 Size: 53 83 186 936 476 2468 382 635 Cuts at: 5494 275 Size: 1451 Fragments arranged by size: 2468 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2861 2877 5103 5501 5775 5922 6333 2861 Size: 16 2226 398 274 147 411 3198 Fragments arranged by size: 3198 2226 411 398 274 147 16 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2548 2578 2872 2902 3393 Size: 30 165 30 1145 30 294 30 491 Cuts at: 3393 3423 3528 3558 1178 Size: 30 105 30 4290 Fragments arranged by size: 4290 1145 491 294 165 105 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2973 3489 3498 3549 3915 1186 Size: 1787 516 9 51 366 3941 Fragments arranged by size: 3941 1787 516 366 51 9 BseYI C'CCAG_C Cuts at: 2073 2486 5873 6369 2073 Size: 413 3387 496 2374 Fragments arranged by size: 3387 2374 496 413 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2391 2544 1163 Size: 573 655 153 5289 Fragments arranged by size: 5289 655 573 153 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4120 4881 5909 1540 Size: 275 186 2119 761 1028 2301 Fragments arranged by size: 2301 2119 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3942 4731 4816 5887 3942 Size: 789 85 1071 4725 Fragments arranged by size: 4725 1071 789 85 BsmI GAATG_Cn' Cuts at: 4181 4274 4181 Size: 93 6577 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3864 3942 3989 4731 4816 5887 1391 Size: 399 2074 78 47 742 85 1071 2174 Fragments arranged by size: 2174 2074 1071 742 399 85 78 47 BspEI T'CCGG_A Cuts at: 2861 6333 2861 Size: 3472 3198 BspHI T'CATG_A Cuts at: 4559 6289 4559 Size: 1730 4940 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4025 844 Size: 3181 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4557 1394 Size: 429 2734 3507 Fragments arranged by size: 3507 2734 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5165 5339 65 Size: 1183 3917 174 1396 Fragments arranged by size: 3917 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5314 5501 1700 Size: 110 3504 187 2869 Fragments arranged by size: 3504 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3094 96 Size: 2998 3672 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6670 BssSI C'ACGA_G Cuts at: 4730 5742 4730 Size: 1012 5658 BstAPI GCAn_nnn'nTGC Cuts at: 794 2405 794 Size: 1611 5059 BstBI TT'CG_AA Cuts at: 5474 5474 Size: 6670 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6670 BstXI CCAn_nnnn'nTGG Cuts at: 2111 2282 2531 2827 2111 Size: 171 249 296 5954 Fragments arranged by size: 5954 296 249 171 BstYI r'GATC_y Cuts at: 1972 2738 3034 3058 4000 4367 4752 4769 6221 Size: 766 296 24 942 367 385 17 1452 Cuts at: 6221 6307 6319 6665 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 942 766 385 367 346 296 86 24 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 3018 3279 3412 3450 3469 Size: 552 939 9 1005 261 133 38 19 Cuts at: 3469 3775 3799 513 Size: 306 24 3384 Fragments arranged by size: 3384 1005 939 552 306 261 133 38 24 19 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3249 487 Size: 683 56 2023 3908 Fragments arranged by size: 3908 2023 683 56 BtsI GCAGTG_nn' Cuts at: 2175 2307 2403 2556 3155 3264 3575 4186 4980 Size: 132 96 153 599 109 311 611 794 Cuts at: 4980 5000 2175 Size: 20 3845 Fragments arranged by size: 3845 794 611 599 311 153 132 109 96 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6670 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6635 DraI TTT'AAA Cuts at: 3974 3974 Size: 6670 DraIII CAC_nnn'GTG Cuts at: 3383 3413 3383 Size: 30 6640 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3453 5677 809 Size: 507 2137 2224 1802 Fragments arranged by size: 2224 2137 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3772 3805 4117 Size: 54 1425 50 275 18 1942 33 312 Cuts at: 4117 5006 6635 8 Size: 889 1629 43 Fragments arranged by size: 1942 1629 1425 889 312 275 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 4117 1537 Size: 275 2305 4090 Fragments arranged by size: 4090 2305 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2908 3718 3892 4600 1353 Size: 1555 810 174 708 3423 Fragments arranged by size: 3423 1555 810 708 174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3660 5257 5631 5777 387 Size: 1064 138 2071 1597 374 146 1280 Fragments arranged by size: 2071 1597 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2871 3003 3552 3579 3628 3666 4733 Size: 393 1239 132 549 27 49 38 1067 Cuts at: 4733 5330 6117 6385 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1239 1067 787 597 549 393 268 132 49 38 27 EcoNI CCTnn'n_nnAGG Cuts at: 3209 3498 3209 Size: 289 6381 EcoO109I rG'GnC_Cy Cuts at: 3437 3446 3503 3634 3860 3861 3914 3437 Size: 9 57 131 226 1 53 6193 Fragments arranged by size: 6193 226 131 57 53 9 1 EcoRI G'AATT_C Cuts at: 3979 4357 3979 Size: 378 6292 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3347 3347 Size: 6670 FspI TGC'GCA Cuts at: 5176 5176 Size: 6670 HaeII r_GCGC'y Cuts at: 1678 2982 5817 1678 Size: 1304 2835 2531 Fragments arranged by size: 2835 2531 1304 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3056 3088 3528 3560 5311 Size: 32 135 32 1484 32 440 32 1751 Cuts at: 5311 5343 5385 5417 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1751 1484 440 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2453 2687 4014 4260 669 Size: 988 281 515 234 1327 246 3079 Fragments arranged by size: 3079 1327 988 515 281 246 234 HindIII A'AGCT_T Cuts at: 748 748 Size: 6670 HpaI GTT'AAC Cuts at: 4260 4260 Size: 6670 KpnI G_GTAC'C Cuts at: 2531 3995 2531 Size: 1464 5206 MfeI C'AATT_G Cuts at: 4269 4269 Size: 6670 MluI A'CGCG_T Cuts at: 6546 6546 Size: 6670 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2267 2830 5784 5968 1305 Size: 962 563 2954 184 2007 Fragments arranged by size: 2954 2007 962 563 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4848 Fragments arranged by size: 4848 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4628 4987 5146 518 Size: 648 3462 359 159 2042 Fragments arranged by size: 3462 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4086 4763 5911 6156 1606 Size: 2480 677 1148 245 2120 Fragments arranged by size: 2480 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3018 3450 513 Size: 552 939 1014 432 3733 Fragments arranged by size: 3733 1014 939 552 432 NdeI CA'TA_TG Cuts at: 387 2110 2356 387 Size: 1723 246 4701 Fragments arranged by size: 4701 1723 246 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6670 NotI GC'GGCC_GC Cuts at: 4117 4117 Size: 6670 NspI r_CATG'y Cuts at: 1132 1229 3400 3715 4028 5573 1132 Size: 97 2171 315 313 1545 2229 Fragments arranged by size: 2229 2171 1545 315 313 97 PasI CC'CwG_GG Cuts at: 1348 3854 1348 Size: 2506 4164 PciI A'CATG_T Cuts at: 5569 5569 Size: 6670 PflMI CCAn_nnn'nTGG Cuts at: 1108 2384 3413 1108 Size: 1276 1029 4365 Fragments arranged by size: 4365 1276 1029 PfoI T'CCnGG_A Cuts at: 3421 3421 Size: 6670 PmeI GTTT'AAAC Cuts at: 3974 3974 Size: 6670 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4739 4771 6252 6284 4739 Size: 32 1481 32 5125 Fragments arranged by size: 5125 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3437 3446 3503 3914 3437 Size: 9 57 411 6193 Fragments arranged by size: 6193 411 57 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6670 PsiI TTA'TAA Cuts at: 4240 4240 Size: 6670 PspOMI G'GGCC_C Cuts at: 3860 3860 Size: 6670 PstI C_TGCA'G Cuts at: 830 2044 4022 830 Size: 1214 1978 3478 Fragments arranged by size: 3478 1978 1214 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6670 SalI G'TCGA_C Cuts at: 1936 4012 1936 Size: 2076 4594 SanDI GG'GwC_CC Cuts at: 3446 3446 Size: 6670 SbfI CC_TGCA'GG Cuts at: 4022 4022 Size: 6670 ScaI AGT'ACT Cuts at: 4918 6630 4918 Size: 1712 4958 SfcI C'TryA_G Cuts at: 826 1045 2029 2040 2056 2313 2512 2743 3054 Size: 219 984 11 16 257 199 231 311 Cuts at: 3054 4018 5153 5834 6025 826 Size: 964 1135 681 191 1471 Fragments arranged by size: 1471 1135 984 964 681 311 257 231 219 199 191 16 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6670 SmaI CCC'GGG Cuts at: 1872 3509 3678 3997 1872 Size: 1637 169 319 4545 Fragments arranged by size: 4545 1637 319 169 SmlI C'TyrA_G Cuts at: 820 1017 1943 2082 4774 5675 5937 820 Size: 197 926 139 2692 901 262 1553 Fragments arranged by size: 2692 1553 926 901 262 197 139 SnaBI TAC'GTA Cuts at: 493 4461 493 Size: 3968 2702 SpeI A'CTAG_T Cuts at: 152 2447 2606 2717 152 Size: 2295 159 111 4105 Fragments arranged by size: 4105 2295 159 111 SphI G_CATG'C Cuts at: 1132 3400 3715 4028 1132 Size: 2268 315 313 3774 Fragments arranged by size: 3774 2268 315 313 SspI AAT'ATT Cuts at: 5 52 4594 5 Size: 47 4542 2081 Fragments arranged by size: 4542 2081 47 StuI AGG'CCT Cuts at: 1517 2333 1517 Size: 816 5854 StyI C'CwwG_G Cuts at: 513 1065 2004 2491 3018 3270 3450 4109 513 Size: 552 939 487 527 252 180 659 3074 Fragments arranged by size: 3074 939 659 552 527 487 252 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2117 2342 2819 3277 3834 4697 4714 Size: 1255 537 225 477 458 557 863 17 Cuts at: 4714 4867 5052 5497 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 863 557 537 477 458 445 225 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3094 4916 6628 Size: 274 80 33 51 1443 1117 1822 1712 Cuts at: 6628 96 Size: 138 Fragments arranged by size: 1822 1712 1443 1117 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2548 2580 2872 2904 3393 3425 6630 6662 2548 Size: 32 292 32 489 32 3205 32 2556 Fragments arranged by size: 3205 2556 489 292 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6670 XbaI T'CTAG_A Cuts at: 3121 4006 3121 Size: 885 5785 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3277 3457 3554 3277 Size: 180 97 6393 Fragments arranged by size: 6393 180 97 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6670 XmaI C'CCGG_G Cuts at: 1870 3507 3676 3995 1870 Size: 1637 169 319 4545 Fragments arranged by size: 4545 1637 319 169 XmnI GAAnn'nnTTC Cuts at: 4799 4799 Size: 6670 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4478 276 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI