Restriction map for pFN21AB4489
 (Circular) (Six-base) MAPSORT of: pFN21AB4489.seq  Check: 1540  from: 1  to: 6106



pFN21AB4489  6106 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:34  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2233    2233 
   Size:       6106
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3916     278 
   Size:         53      83     186     936    2380    2468

  Fragments arranged by size: 

                2468    2380     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3449    1937 
   Size:       1512    4594
 
Acc65I G'GTAC_C

Cuts at:   3427    3427 
   Size:       6106
 
AclI AA'CG_TT

Cuts at:   1563    4233    4606    1563 
   Size:       2670     373    3063

  Fragments arranged by size: 

                3063    2670     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2345    3381    4169    5553    5821    1632 
   Size:        713    1036     788    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1036     788     713     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5909
 
AflIII A'CryG_T

Cuts at:   1203    2589    2758    5005    5982    1203 
   Size:       1386     169    2247     977    1327

  Fragments arranged by size: 

                2247    1386    1327     977     169
 
AgeI A'CCGG_T

Cuts at:   4937    4937 
   Size:       6106
 
AhdI GACnn_n'nnGTC

Cuts at:   4835    4835 
   Size:       6106
 
AleI CACnn'nnGTG

Cuts at:   1166    3343    1166 
   Size:       2177    3929
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2279    3089    1184 
   Size:       1095     810    4201

  Fragments arranged by size: 

                4201    1095     810
 
AlwNI CAG_nnn'CTG

Cuts at:   2937    3141    3184    3349    3404    3849    5421    2937 
   Size:        204      43     165      55     445    1572    3622

  Fragments arranged by size: 

                3622    1572     445     204     165      55      43
 
ApaLI G'TGCA_C

Cuts at:   2608    3011    4163    5319    2608 
   Size:        403    1152    1156    3395

  Fragments arranged by size: 

                3395    1156    1152     403
 
ApoI r'AATT_y

Cuts at:   1493    3415    3641    3793    5902    1493 
   Size:       1922     226     152    2109    1697

  Fragments arranged by size: 

                2109    1922    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4660     160 
   Size:        872    3628    1606

  Fragments arranged by size: 

                3628    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6106
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2188    3431    1870 
   Size:         73     245    1243    4545

  Fragments arranged by size: 

                4545    1243     245      73
 
BamHI G'GATC_C

Cuts at:   3436    3803    3436 
   Size:        367    5739
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3427    4882     618 
   Size:        325     217     627    1640    1455    1842

  Fragments arranged by size: 

                1842    1640    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2179    2814    3377    3425    1391 
   Size:        788     635     563      48    4072

  Fragments arranged by size: 

                4072     788     635     563      48
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3148     928 
   Size:        971    1249    3886

  Fragments arranged by size: 

                3886    1249     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    1987    2021    4297    4331    1903 
   Size:         34      50      34    2276      34    3678

  Fragments arranged by size: 

                3678    2276      50      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3998    5208    3998 
   Size:       1210    4896
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6106
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4717     136 
   Size:        107     122      71     631     605    3045    1525

  Fragments arranged by size: 

                3045    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6101    6101 
   Size:       6106
 
BlpI GC'TnA_GC

Cuts at:   2465    3177    3523    2465 
   Size:        712     346    5048

  Fragments arranged by size: 

                5048     712     346
 
Bme1580I G_kGCm'C

Cuts at:   1790    2612    3015    4167    5323    1790 
   Size:        822     403    1152    1156    2573

  Fragments arranged by size: 

                2573    1156    1152     822     403
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6106
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2593    3184    3837 
   Size:        416      53      83     399      24    1176     591     653

Cuts at:   3837    4795     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1176     958     653     591     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6106
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2189    2354    2397    4766    1239 
   Size:        950     165      43    2369    2579

  Fragments arranged by size: 

                2579    2369     950     165      43
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3134    4231    5096    5394    3134 
   Size:       1097     865     298    3846

  Fragments arranged by size: 

                3846    1097     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2711    4769     882 
   Size:        617    1212    2058    2219

  Fragments arranged by size: 

                2219    2058    1212     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3897     493 
   Size:        711    2693    2702

  Fragments arranged by size: 

                2702    2693     711
 
BsaBI GATnn'nnATC

Cuts at:   6100    6100 
   Size:       6106
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3913    4295    4930     275 
   Size:         53      83     186     936    2380     382     635    1451

  Fragments arranged by size: 

                2380    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3246    4539    4937    5211    5358    5769    3246 
   Size:       1293     398     274     147     411    3583

  Fragments arranged by size: 

                3583    1293     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2579    2609    2612    2642    1178 
   Size:         30     165      30    1176      30       3      30    4642

  Fragments arranged by size: 

                4642    1176     165      30      30      30      30       3
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2822    1186 
   Size:       1636    4470
 
BseYI C'CCAG_C

Cuts at:   2198    2460    2582    2814    3112    3134    5309    5805    2198 
   Size:        262     122     232     298      22    2175     496    2499

  Fragments arranged by size: 

                2499    2175     496     298     262     232     122      22
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2483    2550    3095    1163 
   Size:        573     747      67     545    4174

  Fragments arranged by size: 

                4174     747     573     545      67
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2832    3556    4317    5345    1540 
   Size:        275     186     831     724     761    1028    2301

  Fragments arranged by size: 

                2301    1028     831     761     724     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2433    2564    2612    2828    2895    3015    4167    4252    5323 
   Size:        131      48     216      67     120    1152      85    1071

Cuts at:   5323    2433 
   Size:       3216

  Fragments arranged by size: 

                3216    1152    1071     216     131     120      85      67
                  48
 
BsmI GAATG_Cn'

Cuts at:   3617    3710    3617 
   Size:         93    6013
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2179    2433    2564    2612    2814    2828    2895 
   Size:        399     389     254     131      48     202      14      67

Cuts at:   2895    3015    3377    3425    4167    4252    5323    1391 
   Size:        120     362      48     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     389     362     254     202
                 131     120      85      67      48      48      14
 
BspEI T'CCGG_A

Cuts at:   3246    5769    3246 
   Size:       2523    3583
 
BspHI T'CATG_A

Cuts at:   3995    5725    3995 
   Size:       1730    4376
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2233    3461     844 
   Size:       1389    1228    3489

  Fragments arranged by size: 

                3489    1389    1228
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2223    3993    1394 
   Size:        429     400    1770    3507

  Fragments arranged by size: 

                3507    1770     429     400
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4601    4775      65 
   Size:       1183    3353     174    1396

  Fragments arranged by size: 

                3353    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4750    4937    1700 
   Size:        110    2940     187    2869

  Fragments arranged by size: 

                2940    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6106
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6106
 
BssSI C'ACGA_G

Cuts at:   2318    2432    4166    5178    2318 
   Size:        114    1734    1012    3246

  Fragments arranged by size: 

                3246    1734    1012     114
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3309     794 
   Size:       2515    3591
 
BstBI TT'CG_AA

Cuts at:   4910    4910 
   Size:       6106
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6106
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3345    3345 
   Size:       6106
 
BstYI r'GATC_y

Cuts at:   1972    2302    3129    3436    3803    4188    4205    5657    5743 
   Size:        330     827     307     367     385      17    1452      86

Cuts at:   5743    5755    6101    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     827     385     367     346     330     307
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2157    3202     513 
   Size:        552    1092    1045    3417

  Fragments arranged by size: 

                3417    1092    1045     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    3269     487 
   Size:        683      56    2043    3324

  Fragments arranged by size: 

                3324    2043     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2061    2121    3338    3350    3622    4416    4436    2061 
   Size:         60    1217      12     272     794      20    3731

  Fragments arranged by size: 

                3731    1217     794     272      60      20      12
 
ClaI AT'CG_AT

Cuts at:   1554    2771    1554 
   Size:       1217    4889
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6071
 
DraI TTT'AAA

Cuts at:   3410    3410 
   Size:       6106
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5113     809 
   Size:        507    3797    1802

  Fragments arranged by size: 

                3797    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2297    2475    2613 
   Size:         54    1425      50     275      18     467     178     138

Cuts at:   2613    2728    3553    4442    6071       8 
   Size:        115     825     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     825     467     275     178     138
                 115      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3553    1537 
   Size:        275    1741    4090

  Fragments arranged by size: 

                4090    1741     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2039    2054    2085    3176    4036    1353 
   Size:        686      15      31    1091     860    3423

  Fragments arranged by size: 

                3423    1091     860     686      31      15
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4693    5067    5213     387 
   Size:       1064     138    3104     374     146    1280

  Fragments arranged by size: 

                3104    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2189    2345    2354    2397    3381    4169    4766 
   Size:        393     557     156       9      43     984     788     597

Cuts at:   4766    5553    5821    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     984     788     787     597     557     393     268
                 156      43       9
 
EcoO109I rG'GnC_Cy

Cuts at:   2260    2260 
   Size:       6106
 
EcoRI G'AATT_C

Cuts at:   3415    3793    3415 
   Size:        378    5728
 
FspI TGC'GCA

Cuts at:   4612    4612 
   Size:       6106
 
HaeII r_GCGC'y

Cuts at:   1678    2296    2493    5253    1678 
   Size:        618     197    2760    2531

  Fragments arranged by size: 

                2760    2531     618     197
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2266    2294    2298    2326    2647 
   Size:         32     135      32     694      28       4      28     321

Cuts at:   2647    2679    3361    3393    4747    4779    4821    4853    1373 
   Size:         32     682      32    1354      32      42      32    2626

  Fragments arranged by size: 

                2626    1354     694     682     321     135      42      32
                  32      32      32      32      32      28      28       4
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2677    3450    3696     669 
   Size:        988     281     739     773     246    3079

  Fragments arranged by size: 

                3079     988     773     739     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6106
 
HpaI GTT'AAC

Cuts at:   3696    3696 
   Size:       6106
 
KpnI G_GTAC'C

Cuts at:   3431    3431 
   Size:       6106
 
MfeI C'AATT_G

Cuts at:   3705    3705 
   Size:       6106
 
MluI A'CGCG_T

Cuts at:   2589    5982    2589 
   Size:       3393    2713
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    5220    5404    1305 
   Size:       3915     184    2007

  Fragments arranged by size: 

                3915    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2477    2730      10 
   Size:         54    1425     343     645     253    3386

  Fragments arranged by size: 

                3386    1425     645     343     253      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3071    3330    3343    4064    4423    4582     518 
   Size:        648    1905     259      13     721     359     159    2042

  Fragments arranged by size: 

                2042    1905     721     648     359     259     159      13
            
 
MspA1I CmG'CkG

Cuts at:   1606    2184    2332    2750    3138    3522    4199    5347    5592 
   Size:        578     148     418     388     384     677    1148     245

Cuts at:   5592    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     677     578     418     388     384     245
                 148
 
NcoI C'CATG_G

Cuts at:    513    1065    2157     513 
   Size:        552    1092    4462

  Fragments arranged by size: 

                4462    1092     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       6106
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6106
 
NotI GC'GGCC_GC

Cuts at:   3553    3553 
   Size:       6106
 
NspI r_CATG'y

Cuts at:   1132    1229    2762    3464    5009    1132 
   Size:         97    1533     702    1545    2229

  Fragments arranged by size: 

                2229    1545    1533     702      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6106
 
PciI A'CATG_T

Cuts at:   2758    5005    2758 
   Size:       2247    3859
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6106
 
PmeI GTTT'AAAC

Cuts at:   3410    3410 
   Size:       6106
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4175    4207    5688    5720    4175 
   Size:         32    1481      32    4561

  Fragments arranged by size: 

                4561    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6106
 
PsiI TTA'TAA

Cuts at:   3676    3676 
   Size:       6106
 
PstI C_TGCA'G

Cuts at:    830    2244    2310    3146    3345    3458     830 
   Size:       1414      66     836     199     113    3478

  Fragments arranged by size: 

                3478    1414     836     199     113      66
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6106
 
PvuII CAG'CTG

Cuts at:   2184    2750    2184 
   Size:        566    5540
 
SalI G'TCGA_C

Cuts at:   1936    3448    1936 
   Size:       1512    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   3176    3176 
   Size:       6106
 
SbfI CC_TGCA'GG

Cuts at:   3458    3458 
   Size:       6106
 
ScaI AGT'ACT

Cuts at:   2418    4354    6066    2418 
   Size:       1936    1712    2458

  Fragments arranged by size: 

                2458    1936    1712
 
SfcI C'TryA_G

Cuts at:    826    1045    2240    2306    2736    3142    3341    3454    4589 
   Size:        219    1195      66     430     406     199     113    1135

Cuts at:   4589    5270    5461     826 
   Size:        681     191    1471

  Fragments arranged by size: 

                1471    1195    1135     681     430     406     219     199
                 191     113      66
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6106
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6106
 
SmaI CCC'GGG

Cuts at:   1872    2190    3433    1872 
   Size:        318    1243    4545

  Fragments arranged by size: 

                4545    1243     318
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3149    4210    5111    5373     820 
   Size:        197     926    1206    1061     901     262    1553

  Fragments arranged by size: 

                1553    1206    1061     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3897     493 
   Size:       3404    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6106
 
SphI G_CATG'C

Cuts at:   1132    3464    1132 
   Size:       2332    3774
 
SspI AAT'ATT

Cuts at:      5      52    4030       5 
   Size:         47    3978    2081

  Fragments arranged by size: 

                3978    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6106
 
StyI C'CwwG_G

Cuts at:    513    1065    2157    3545     513 
   Size:        552    1092    1388    3074

  Fragments arranged by size: 

                3074    1388    1092     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2596    2818    3126    4133    4150    4303    4488 
   Size:       1255    1016     222     308    1007      17     153     185

Cuts at:   4488    4933     325 
   Size:        445    1498

  Fragments arranged by size: 

                1498    1255    1016    1007     445     308     222     185
                 153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2416    3018    3239 
   Size:        274      80      33      51    1443     439     602     221

Cuts at:   3239    4352    6064      96 
   Size:       1113    1712     138

  Fragments arranged by size: 

                1712    1443    1113     602     439     274     221     138
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2579    2611    2994    3026    6066    6098    2579 
   Size:         32     383      32    3040      32    2587

  Fragments arranged by size: 

                3040    2587     383      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6106
 
XbaI T'CTAG_A

Cuts at:   3442    3442 
   Size:       6106
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2164    2164 
   Size:       6106
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6106
 
XmaI C'CCGG_G

Cuts at:   1870    2188    3431    1870 
   Size:        318    1243    4545

  Fragments arranged by size: 

                4545    1243     318
 
XmnI GAAnn'nnTTC

Cuts at:   2641    2933    4235    2641 
   Size:        292    1302    4512

  Fragments arranged by size: 

                4512    1302     292
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3914     276 
   Size:         53      83     186     936    2380    2468

  Fragments arranged by size: 

                2468    2380     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    PvuII     SalI
     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    RsrII     SacI    SacII    SanDI    SexAI
     SfiI     SfoI     SrfI     SwaI
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