(Circular) (Six-base) MAPSORT of: pFN21AB4489.seq Check: 1540 from: 1 to: 6106 pFN21AB4489 6106 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2233 2233 Size: 6106 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3916 278 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3449 1937 Size: 1512 4594 Acc65I G'GTAC_C Cuts at: 3427 3427 Size: 6106 AclI AA'CG_TT Cuts at: 1563 4233 4606 1563 Size: 2670 373 3063 Fragments arranged by size: 3063 2670 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 3381 4169 5553 5821 1632 Size: 713 1036 788 1384 268 1917 Fragments arranged by size: 1917 1384 1036 788 713 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5909 AflIII A'CryG_T Cuts at: 1203 2589 2758 5005 5982 1203 Size: 1386 169 2247 977 1327 Fragments arranged by size: 2247 1386 1327 977 169 AgeI A'CCGG_T Cuts at: 4937 4937 Size: 6106 AhdI GACnn_n'nnGTC Cuts at: 4835 4835 Size: 6106 AleI CACnn'nnGTG Cuts at: 1166 3343 1166 Size: 2177 3929 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2279 3089 1184 Size: 1095 810 4201 Fragments arranged by size: 4201 1095 810 AlwNI CAG_nnn'CTG Cuts at: 2937 3141 3184 3349 3404 3849 5421 2937 Size: 204 43 165 55 445 1572 3622 Fragments arranged by size: 3622 1572 445 204 165 55 43 ApaLI G'TGCA_C Cuts at: 2608 3011 4163 5319 2608 Size: 403 1152 1156 3395 Fragments arranged by size: 3395 1156 1152 403 ApoI r'AATT_y Cuts at: 1493 3415 3641 3793 5902 1493 Size: 1922 226 152 2109 1697 Fragments arranged by size: 2109 1922 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4660 160 Size: 872 3628 1606 Fragments arranged by size: 3628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 2188 3431 1870 Size: 73 245 1243 4545 Fragments arranged by size: 4545 1243 245 73 BamHI G'GATC_C Cuts at: 3436 3803 3436 Size: 367 5739 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3427 4882 618 Size: 325 217 627 1640 1455 1842 Fragments arranged by size: 1842 1640 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2179 2814 3377 3425 1391 Size: 788 635 563 48 4072 Fragments arranged by size: 4072 788 635 563 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3148 928 Size: 971 1249 3886 Fragments arranged by size: 3886 1249 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4297 4331 1903 Size: 34 50 34 2276 34 3678 Fragments arranged by size: 3678 2276 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3998 5208 3998 Size: 1210 4896 BclI T'GATC_A Cuts at: 1548 1548 Size: 6106 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4717 136 Size: 107 122 71 631 605 3045 1525 Fragments arranged by size: 3045 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6101 6101 Size: 6106 BlpI GC'TnA_GC Cuts at: 2465 3177 3523 2465 Size: 712 346 5048 Fragments arranged by size: 5048 712 346 Bme1580I G_kGCm'C Cuts at: 1790 2612 3015 4167 5323 1790 Size: 822 403 1152 1156 2573 Fragments arranged by size: 2573 1156 1152 822 403 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2593 3184 3837 Size: 416 53 83 399 24 1176 591 653 Cuts at: 3837 4795 442 Size: 958 1753 Fragments arranged by size: 1753 1176 958 653 591 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2354 2397 4766 1239 Size: 950 165 43 2369 2579 Fragments arranged by size: 2579 2369 950 165 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3134 4231 5096 5394 3134 Size: 1097 865 298 3846 Fragments arranged by size: 3846 1097 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2711 4769 882 Size: 617 1212 2058 2219 Fragments arranged by size: 2219 2058 1212 617 BsaAI yAC'GTr Cuts at: 493 1204 3897 493 Size: 711 2693 2702 Fragments arranged by size: 2702 2693 711 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3913 4295 4930 275 Size: 53 83 186 936 2380 382 635 1451 Fragments arranged by size: 2380 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3246 4539 4937 5211 5358 5769 3246 Size: 1293 398 274 147 411 3583 Fragments arranged by size: 3583 1293 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2579 2609 2612 2642 1178 Size: 30 165 30 1176 30 3 30 4642 Fragments arranged by size: 4642 1176 165 30 30 30 30 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2822 1186 Size: 1636 4470 BseYI C'CCAG_C Cuts at: 2198 2460 2582 2814 3112 3134 5309 5805 2198 Size: 262 122 232 298 22 2175 496 2499 Fragments arranged by size: 2499 2175 496 298 262 232 122 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2483 2550 3095 1163 Size: 573 747 67 545 4174 Fragments arranged by size: 4174 747 573 545 67 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2832 3556 4317 5345 1540 Size: 275 186 831 724 761 1028 2301 Fragments arranged by size: 2301 1028 831 761 724 275 186 BsiHKAI G_wGCw'C Cuts at: 2433 2564 2612 2828 2895 3015 4167 4252 5323 Size: 131 48 216 67 120 1152 85 1071 Cuts at: 5323 2433 Size: 3216 Fragments arranged by size: 3216 1152 1071 216 131 120 85 67 48 BsmI GAATG_Cn' Cuts at: 3617 3710 3617 Size: 93 6013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2179 2433 2564 2612 2814 2828 2895 Size: 399 389 254 131 48 202 14 67 Cuts at: 2895 3015 3377 3425 4167 4252 5323 1391 Size: 120 362 48 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 389 362 254 202 131 120 85 67 48 48 14 BspEI T'CCGG_A Cuts at: 3246 5769 3246 Size: 2523 3583 BspHI T'CATG_A Cuts at: 3995 5725 3995 Size: 1730 4376 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2233 3461 844 Size: 1389 1228 3489 Fragments arranged by size: 3489 1389 1228 BsrBI CCG'CTC Cuts at: 1394 1823 2223 3993 1394 Size: 429 400 1770 3507 Fragments arranged by size: 3507 1770 429 400 BsrDI GCAATG_nn' Cuts at: 65 1248 4601 4775 65 Size: 1183 3353 174 1396 Fragments arranged by size: 3353 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4750 4937 1700 Size: 110 2940 187 2869 Fragments arranged by size: 2940 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6106 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6106 BssSI C'ACGA_G Cuts at: 2318 2432 4166 5178 2318 Size: 114 1734 1012 3246 Fragments arranged by size: 3246 1734 1012 114 BstAPI GCAn_nnn'nTGC Cuts at: 794 3309 794 Size: 2515 3591 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6106 BstXI CCAn_nnnn'nTGG Cuts at: 3345 3345 Size: 6106 BstYI r'GATC_y Cuts at: 1972 2302 3129 3436 3803 4188 4205 5657 5743 Size: 330 827 307 367 385 17 1452 86 Cuts at: 5743 5755 6101 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 827 385 367 346 330 307 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2157 3202 513 Size: 552 1092 1045 3417 Fragments arranged by size: 3417 1092 1045 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3269 487 Size: 683 56 2043 3324 Fragments arranged by size: 3324 2043 683 56 BtsI GCAGTG_nn' Cuts at: 2061 2121 3338 3350 3622 4416 4436 2061 Size: 60 1217 12 272 794 20 3731 Fragments arranged by size: 3731 1217 794 272 60 20 12 ClaI AT'CG_AT Cuts at: 1554 2771 1554 Size: 1217 4889 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 3410 3410 Size: 6106 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2297 2475 2613 Size: 54 1425 50 275 18 467 178 138 Cuts at: 2613 2728 3553 4442 6071 8 Size: 115 825 889 1629 43 Fragments arranged by size: 1629 1425 889 825 467 275 178 138 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3553 1537 Size: 275 1741 4090 Fragments arranged by size: 4090 1741 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2039 2054 2085 3176 4036 1353 Size: 686 15 31 1091 860 3423 Fragments arranged by size: 3423 1091 860 686 31 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4693 5067 5213 387 Size: 1064 138 3104 374 146 1280 Fragments arranged by size: 3104 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2345 2354 2397 3381 4169 4766 Size: 393 557 156 9 43 984 788 597 Cuts at: 4766 5553 5821 1239 Size: 787 268 1524 Fragments arranged by size: 1524 984 788 787 597 557 393 268 156 43 9 EcoO109I rG'GnC_Cy Cuts at: 2260 2260 Size: 6106 EcoRI G'AATT_C Cuts at: 3415 3793 3415 Size: 378 5728 FspI TGC'GCA Cuts at: 4612 4612 Size: 6106 HaeII r_GCGC'y Cuts at: 1678 2296 2493 5253 1678 Size: 618 197 2760 2531 Fragments arranged by size: 2760 2531 618 197 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2266 2294 2298 2326 2647 Size: 32 135 32 694 28 4 28 321 Cuts at: 2647 2679 3361 3393 4747 4779 4821 4853 1373 Size: 32 682 32 1354 32 42 32 2626 Fragments arranged by size: 2626 1354 694 682 321 135 42 32 32 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 2677 3450 3696 669 Size: 988 281 739 773 246 3079 Fragments arranged by size: 3079 988 773 739 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6106 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KpnI G_GTAC'C Cuts at: 3431 3431 Size: 6106 MfeI C'AATT_G Cuts at: 3705 3705 Size: 6106 MluI A'CGCG_T Cuts at: 2589 5982 2589 Size: 3393 2713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5220 5404 1305 Size: 3915 184 2007 Fragments arranged by size: 3915 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2477 2730 10 Size: 54 1425 343 645 253 3386 Fragments arranged by size: 3386 1425 645 343 253 54 MslI CAynn'nnrTG Cuts at: 518 1166 3071 3330 3343 4064 4423 4582 518 Size: 648 1905 259 13 721 359 159 2042 Fragments arranged by size: 2042 1905 721 648 359 259 159 13 MspA1I CmG'CkG Cuts at: 1606 2184 2332 2750 3138 3522 4199 5347 5592 Size: 578 148 418 388 384 677 1148 245 Cuts at: 5592 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 578 418 388 384 245 148 NcoI C'CATG_G Cuts at: 513 1065 2157 513 Size: 552 1092 4462 Fragments arranged by size: 4462 1092 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NspI r_CATG'y Cuts at: 1132 1229 2762 3464 5009 1132 Size: 97 1533 702 1545 2229 Fragments arranged by size: 2229 1545 1533 702 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6106 PciI A'CATG_T Cuts at: 2758 5005 2758 Size: 2247 3859 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6106 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4175 4207 5688 5720 4175 Size: 32 1481 32 4561 Fragments arranged by size: 4561 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6106 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PstI C_TGCA'G Cuts at: 830 2244 2310 3146 3345 3458 830 Size: 1414 66 836 199 113 3478 Fragments arranged by size: 3478 1414 836 199 113 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 PvuII CAG'CTG Cuts at: 2184 2750 2184 Size: 566 5540 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SapI GCTCTTCn'nnn_ Cuts at: 3176 3176 Size: 6106 SbfI CC_TGCA'GG Cuts at: 3458 3458 Size: 6106 ScaI AGT'ACT Cuts at: 2418 4354 6066 2418 Size: 1936 1712 2458 Fragments arranged by size: 2458 1936 1712 SfcI C'TryA_G Cuts at: 826 1045 2240 2306 2736 3142 3341 3454 4589 Size: 219 1195 66 430 406 199 113 1135 Cuts at: 4589 5270 5461 826 Size: 681 191 1471 Fragments arranged by size: 1471 1195 1135 681 430 406 219 199 191 113 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 2190 3433 1872 Size: 318 1243 4545 Fragments arranged by size: 4545 1243 318 SmlI C'TyrA_G Cuts at: 820 1017 1943 3149 4210 5111 5373 820 Size: 197 926 1206 1061 901 262 1553 Fragments arranged by size: 1553 1206 1061 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3897 493 Size: 3404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 3464 1132 Size: 2332 3774 SspI AAT'ATT Cuts at: 5 52 4030 5 Size: 47 3978 2081 Fragments arranged by size: 3978 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6106 StyI C'CwwG_G Cuts at: 513 1065 2157 3545 513 Size: 552 1092 1388 3074 Fragments arranged by size: 3074 1388 1092 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2596 2818 3126 4133 4150 4303 4488 Size: 1255 1016 222 308 1007 17 153 185 Cuts at: 4488 4933 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1016 1007 445 308 222 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2416 3018 3239 Size: 274 80 33 51 1443 439 602 221 Cuts at: 3239 4352 6064 96 Size: 1113 1712 138 Fragments arranged by size: 1712 1443 1113 602 439 274 221 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2579 2611 2994 3026 6066 6098 2579 Size: 32 383 32 3040 32 2587 Fragments arranged by size: 3040 2587 383 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6106 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2164 2164 Size: 6106 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6106 XmaI C'CCGG_G Cuts at: 1870 2188 3431 1870 Size: 318 1243 4545 Fragments arranged by size: 4545 1243 318 XmnI GAAnn'nnTTC Cuts at: 2641 2933 4235 2641 Size: 292 1302 4512 Fragments arranged by size: 4512 1302 292 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3914 276 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI