(Circular) (Six-base) MAPSORT of: pFN21AB4046.seq Check: 4247 from: 1 to: 6040 pFN21AB4046 6040 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3100 3100 Size: 6040 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3090 3167 3383 1937 Size: 1153 77 216 4594 Fragments arranged by size: 4594 1153 216 77 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2030 2711 4103 5487 5755 1632 Size: 398 681 1392 1384 268 1917 Fragments arranged by size: 1917 1392 1384 681 398 268 AfeI AGC'GCT Cuts at: 2128 2128 Size: 6040 AflII C'TTAA_G Cuts at: 820 1017 3224 820 Size: 197 2207 3636 Fragments arranged by size: 3636 2207 197 AflIII A'CryG_T Cuts at: 1203 4939 5916 1203 Size: 3736 977 1327 Fragments arranged by size: 3736 1327 977 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 3226 4769 3226 Size: 1543 4497 AleI CACnn'nnGTG Cuts at: 1166 3310 1166 Size: 2144 3896 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2534 1184 Size: 1350 4690 AlwNI CAG_nnn'CTG Cuts at: 2105 3783 5355 2105 Size: 1678 1572 2790 Fragments arranged by size: 2790 1678 1572 ApaLI G'TGCA_C Cuts at: 3020 4097 5253 3020 Size: 1077 1156 3807 Fragments arranged by size: 3807 1156 1077 ApoI r'AATT_y Cuts at: 1493 3349 3575 3727 5836 1493 Size: 1856 226 152 2109 1697 Fragments arranged by size: 2109 1856 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 2157 3365 1870 Size: 73 214 1208 4545 Fragments arranged by size: 4545 1208 214 73 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2260 2518 3298 3361 4816 Size: 325 217 627 473 258 780 63 1455 Cuts at: 4816 618 Size: 1842 Fragments arranged by size: 1842 1455 780 627 473 325 258 217 63 BanII G_rGCy'C Cuts at: 1391 2406 2829 2847 3359 1391 Size: 1015 423 18 512 4072 Fragments arranged by size: 4072 1015 512 423 18 BbeI G_GCGC'C Cuts at: 3302 3302 Size: 6040 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2584 928 Size: 971 685 4384 Fragments arranged by size: 4384 971 685 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4231 4265 1903 Size: 34 50 34 2210 34 3678 Fragments arranged by size: 3678 2210 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 1548 Size: 6040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2851 4651 136 Size: 107 122 71 631 605 1179 1800 1525 Fragments arranged by size: 1800 1525 1179 631 605 122 107 71 BglII A'GATC_T Cuts at: 2925 6035 2925 Size: 3110 2930 BlpI GC'TnA_GC Cuts at: 2400 2835 3457 2400 Size: 435 622 4983 Fragments arranged by size: 4983 622 435 Bme1580I G_kGCm'C Cuts at: 1790 2265 2439 3024 4101 5257 1790 Size: 475 174 585 1077 1156 2573 Fragments arranged by size: 2573 1156 1077 585 475 174 BmgBI CAC'GTC Cuts at: 1318 3312 1318 Size: 1994 4046 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3771 4729 442 Size: 416 53 83 399 24 2354 958 1753 Fragments arranged by size: 2354 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3095 3095 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2660 2861 4700 1239 Size: 980 441 201 1839 2579 Fragments arranged by size: 2579 1839 980 441 201 Bpu10I CC'TnA_GC Cuts at: 2504 2769 2504 Size: 265 5775 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2914 2975 4165 5030 5328 2914 Size: 61 1190 865 298 3626 Fragments arranged by size: 3626 1190 865 298 61 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4703 882 Size: 617 3204 2219 Fragments arranged by size: 3204 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3831 493 Size: 711 2627 2702 Fragments arranged by size: 2702 2627 711 BsaBI GATnn'nnATC Cuts at: 2324 6034 2324 Size: 3710 2330 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2184 2757 3299 3847 Size: 53 83 186 936 651 573 542 548 Cuts at: 3847 4229 4864 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 651 635 573 548 542 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3135 4473 4871 5145 5292 5703 3135 Size: 1338 398 274 147 411 3472 Fragments arranged by size: 3472 1338 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2834 2864 1178 Size: 30 165 30 1431 30 4354 Fragments arranged by size: 4354 1431 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2456 1186 Size: 883 387 4770 Fragments arranged by size: 4770 883 387 BseYI C'CCAG_C Cuts at: 2098 2283 2574 2581 3030 3102 3145 5243 5739 Size: 185 291 7 449 72 43 2098 496 Cuts at: 5739 2098 Size: 2399 Fragments arranged by size: 2399 2098 496 449 291 185 72 43 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3182 1163 Size: 573 1446 4021 Fragments arranged by size: 4021 1446 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2619 2888 3490 4251 5279 1540 Size: 275 186 618 269 602 761 1028 2301 Fragments arranged by size: 2301 1028 761 618 602 275 269 186 BsiHKAI G_wGCw'C Cuts at: 2406 2829 3024 3100 4101 4186 5257 2406 Size: 423 195 76 1001 85 1071 3189 Fragments arranged by size: 3189 1071 1001 423 195 85 76 BsmI GAATG_Cn' Cuts at: 2071 3551 3644 2071 Size: 1480 93 4467 Fragments arranged by size: 4467 1480 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2438 2722 2438 Size: 284 5756 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2265 2406 2439 2829 2847 3024 3100 Size: 399 475 141 33 390 18 177 76 Cuts at: 3100 3359 4101 4186 5257 1391 Size: 259 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 475 399 390 259 177 141 85 76 33 18 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 3929 5659 3929 Size: 1730 4310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3057 3100 3395 844 Size: 2213 43 295 3489 Fragments arranged by size: 3489 2213 295 43 BsrBI CCG'CTC Cuts at: 1394 1823 2203 3927 1394 Size: 429 380 1724 3507 Fragments arranged by size: 3507 1724 429 380 BsrDI GCAATG_nn' Cuts at: 65 1248 2511 4535 4709 65 Size: 1183 1263 2024 174 1396 Fragments arranged by size: 2024 1396 1263 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2311 4684 4871 1700 Size: 110 501 2373 187 2869 Fragments arranged by size: 2869 2373 501 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6040 BssHII G'CGCG_C Cuts at: 1925 2561 1925 Size: 636 5404 BssSI C'ACGA_G Cuts at: 2438 4100 5112 2438 Size: 1662 1012 3366 Fragments arranged by size: 3366 1662 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3019 794 Size: 2225 3815 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6040 BstXI CCAn_nnnn'nTGG Cuts at: 3205 3312 3205 Size: 107 5933 BstYI r'GATC_y Cuts at: 1972 2925 3370 3737 4122 4139 5591 5677 5689 Size: 953 445 367 385 17 1452 86 12 Cuts at: 5689 6035 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 953 445 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3168 3168 Size: 6040 BtgI C'CryG_G Cuts at: 513 1065 2268 2613 2619 2965 513 Size: 552 1203 345 6 346 3588 Fragments arranged by size: 3588 1203 552 346 345 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2320 2443 487 Size: 683 56 1094 123 4084 Fragments arranged by size: 4084 1094 683 123 56 BtsI GCAGTG_nn' Cuts at: 3556 4350 4370 3556 Size: 794 20 5226 Fragments arranged by size: 5226 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6005 DraI TTT'AAA Cuts at: 3344 3344 Size: 6040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5047 809 Size: 507 3731 1802 Fragments arranged by size: 3731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2616 2820 2852 Size: 54 1425 50 275 18 786 204 32 Cuts at: 2852 2885 2947 3487 4376 6005 8 Size: 33 62 540 889 1629 43 Fragments arranged by size: 1629 1425 889 786 540 275 204 62 54 50 43 33 32 18 EagI C'GGCC_G Cuts at: 1537 1812 2616 2885 3487 1537 Size: 275 804 269 602 4090 Fragments arranged by size: 4090 804 602 275 269 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2411 3970 1353 Size: 734 324 1559 3423 Fragments arranged by size: 3423 1559 734 324 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4627 5001 5147 387 Size: 1064 138 3038 374 146 1280 Fragments arranged by size: 3038 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2404 2827 2404 Size: 423 5617 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2219 2660 2711 2861 4103 4700 Size: 393 398 189 441 51 150 1242 597 Cuts at: 4700 5487 5755 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1242 787 597 441 398 393 268 189 150 51 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 FspI TGC'GCA Cuts at: 4546 4546 Size: 6040 HaeII r_GCGC'y Cuts at: 1678 2046 2130 2365 2582 3302 5187 1678 Size: 368 84 235 217 720 1885 2531 Fragments arranged by size: 2531 1885 720 368 235 217 84 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2225 2257 3212 3244 4681 Size: 32 135 32 653 32 955 32 1437 Cuts at: 4681 4713 4755 4787 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1437 955 653 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3384 3630 669 Size: 988 281 1446 246 3079 Fragments arranged by size: 3079 1446 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KasI G'GCGC_C Cuts at: 3298 3298 Size: 6040 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 3639 3639 Size: 6040 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5154 5338 1305 Size: 3849 184 2007 Fragments arranged by size: 3849 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2854 10 Size: 54 1425 343 1022 3196 Fragments arranged by size: 3196 1425 1022 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2516 3310 3998 4357 4516 518 Size: 648 1350 794 688 359 159 2042 Fragments arranged by size: 2042 1350 794 688 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2251 2270 2574 2615 2686 2896 3034 3055 Size: 645 19 304 41 71 210 138 21 Cuts at: 3055 3106 3456 4133 5281 5526 1606 Size: 51 350 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 645 350 304 245 210 138 71 51 41 21 19 NaeI GCC'GGC Cuts at: 2313 2313 Size: 6040 NarI GG'CG_CC Cuts at: 3299 3299 Size: 6040 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5488 NdeI CA'TA_TG Cuts at: 387 387 Size: 6040 NgoMIV G'CCGG_C Cuts at: 2311 2311 Size: 6040 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 3398 4943 1132 Size: 97 2169 1545 2229 Fragments arranged by size: 2229 2169 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6040 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 2105 1108 Size: 997 5043 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 5622 5654 4109 Size: 32 1481 32 4495 Fragments arranged by size: 4495 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PstI C_TGCA'G Cuts at: 830 2026 2223 3054 3111 3392 830 Size: 1196 197 831 57 281 3478 Fragments arranged by size: 3478 1196 831 281 197 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 PvuII CAG'CTG Cuts at: 3034 3106 3034 Size: 72 5968 SacI G_AGCT'C Cuts at: 2406 2829 2406 Size: 423 5617 SacII CC_GC'GG Cuts at: 2271 2616 2271 Size: 345 5695 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SapI GCTCTTCn'nnn_ Cuts at: 2411 2411 Size: 6040 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 2666 4288 6000 2666 Size: 1622 1712 2706 Fragments arranged by size: 2706 1712 1622 SfcI C'TryA_G Cuts at: 826 1045 2022 2219 3050 3107 3388 4523 5204 Size: 219 977 197 831 57 281 1135 681 Cuts at: 5204 5395 826 Size: 191 1471 Fragments arranged by size: 1471 1135 977 831 681 281 219 197 191 57 SfoI GGC'GCC Cuts at: 3300 3300 Size: 6040 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 2311 1700 Size: 611 5429 SmaI CCC'GGG Cuts at: 1872 2159 3367 1872 Size: 287 1208 4545 Fragments arranged by size: 4545 1208 287 SmlI C'TyrA_G Cuts at: 820 1017 1943 2929 2990 3224 4144 5045 5307 Size: 197 926 986 61 234 920 901 262 Cuts at: 5307 820 Size: 1553 Fragments arranged by size: 1553 986 926 920 901 262 234 197 61 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 2767 2861 3062 1517 Size: 1250 94 201 4495 Fragments arranged by size: 4495 1250 201 94 StyI C'CwwG_G Cuts at: 513 1065 3198 3479 513 Size: 552 2133 281 3074 Fragments arranged by size: 3074 2133 552 281 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3044 3084 3197 4067 4084 4237 4422 Size: 1255 1464 40 113 870 17 153 185 Cuts at: 4422 4867 325 Size: 445 1498 Fragments arranged by size: 1498 1464 1255 870 445 185 153 113 40 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2664 4286 5998 Size: 274 80 33 51 1443 687 1622 1712 Cuts at: 5998 96 Size: 138 Fragments arranged by size: 1712 1622 1443 687 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2834 2866 6000 6032 2834 Size: 32 3134 32 2842 Fragments arranged by size: 3134 2842 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6040 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2862 2862 Size: 6040 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 2157 3365 1870 Size: 287 1208 4545 Fragments arranged by size: 4545 1208 287 XmnI GAAnn'nnTTC Cuts at: 2166 4169 2166 Size: 2003 4037 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI